• Title/Summary/Keyword: Radiation Effect

Search Result 3,580, Processing Time 0.029 seconds

Antioxidative and Cellular Protective Effects of Lysimachia christinae Hance Extract and Fractions (금전초 추출물 및 분획물의 항산화 활성 및 세포 보호 효과)

  • Kim, A Rang;Jung, Min Chul;Jeong, Hye In;Song, Dong Gi;Seo, Young Bin;Jeon, Young Hee;Park, So Hyun;Shin, Hyuk Soo;Lee, Sang Lae;Park, Soo Nam
    • Applied Chemistry for Engineering
    • /
    • v.29 no.2
    • /
    • pp.176-184
    • /
    • 2018
  • In the present study, we investigated the antioxidative properties, cellular protective effects and component analyses of 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Lysimachia christinae Hance (L. christinae Hance). In the evaluation of antioxidative properties, the free radical scavenging activities ($FSC_{50}$) of 50% ethanol extract, ethyl acetate fraction and aglycone fraction were 146.8, 22.2 and $27.2{\mu}g/mL$, respectively and total antioxidant capacities ($OSC_{50}$) were 29.3, 2.9 and $4.5{\mu}g/mL$, respectively. The ethyl acetate fraction showed the highest free radical scavenging activity and total antioxidant capacity. Also, the cellular protective effects (${\tau}_{50}$) of 50% ethanol extract, ethyl acetate fraction and aglycone fraction on $^1O_2$ induced photohemolysis of human erythrocytes were 26.9, 57.5 and 103.9 min at $5{\mu}g/mL$, respectively. In particular, ${\tau}_{50}$ of the aglycone fraction exhibited a higher cellular protective effect than that of (+)-${\alpha}$-tocopherol (37.7 min). The cell viability of the ethyl acetate fraction on the UVB-induced cell damage increased up to 90.1%. In addition, the ethyl acetate fraction ($5-25{\mu}g/mL$) showed cellular protective effects on the $H_2O_2-induced$ cell damages in a dose-dependent manner. TLC, HPLC, UV-vis spectroscopy and LC-MS were used to analyse components of the ethyl acetate fraction and the main components were quercetin, kaempferol and their glycosides. In conclusion, L. christinae Hance extract/fraction can function as antioxidants to protect the skin exposed to UV radiation and may also be used as a novel functional cosmetic material, for example, an antioxidant against skin photoaging.

Antioxidative Activity and Componential Analysis of Chamaecyparis obtusa Leaf Extract (편백나무 잎 추출물의 항산화 활성과 성분분석에 관한 연구)

  • Lee, Dong Sook;Lim, Myoung Sun;Kwan, Soon Sik;Kim, Sun Young;Park, Soo Nam
    • Applied Chemistry for Engineering
    • /
    • v.23 no.1
    • /
    • pp.93-99
    • /
    • 2012
  • In this study, the evaluation of antioxidative activity and componential analysis of C. obtusa leaf extracts was carried out. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of C. obtusa leaf extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction ($OSC_{50}$; 0.22 ${\mu}g/mL$) and aglycone fraction of C. obtusa leaf extracts (0.20 ${\mu}g/mL$) showed about 7 times more prominent ROS scavenging activity than L-ascorbic acid (1.50 ${\mu}g/mL$). The cellular protective effects of fractions obtained from C. obtusa leaf extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction and aglycone fraction of C. obtusa leaf extracts showed the cellular protective effects in a concentration dependent manner (5~25 ${\mu}g/mL$). The inhibitory effect ($IC_{50}$) of ethyl acetate fraction and aglycone fraction on tyrosinase exhibited 74.43 and 53.80 ${\mu}g/mL$, repectively. The aglycone fraction showed four times higher tyrosinase inhibitory effect than arbutin (226.88 ${\mu}g/mL$), known as a whitening agent. The aglycone fraction of C. obtusa leaf extracts showed three bands in TLC chromatogram and three peaks in HPLC chromatogram (360 nm). Three compounds were identified as taxifolin, quercetin and kaempferol. These results indicate that the fractions of C. obtusa leaf extracts can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against reactive oxygen species. The fractions of C. obtusa leaf extracts can be applicable to new functional cosmetics for antioxidan and whitening effects.

Genotypic Variation of Early Growth Vigor and Indicator Traits for its Indirect Selection in Rice (벼 유모활력의 품종 변이와 간접 선발을 위한 초기생육 지표형질 탐색)

  • Fu, Jin-Dong;Lee, Byun-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.52 no.4
    • /
    • pp.429-438
    • /
    • 2007
  • Early growth vigor(EGV) is one of the physiological characteristics that may contribute to the increase of genetic yield potential and radiation use efficiency by closing the canopy earlier. To estimate the genotypic variation of EGV, determine the relationships among the related traits, and identify the rapidly growing genotypes and indirect indicator for selection in breeding program, the evaluation of EGV and EGV-related traits was conducted for a total of 140 rice varieties consisting of 101 Korean, 25 Northern China and 14 IRRI-bred rice varieties in a serial sowing experiment in plastic rain shelter and plastic-covered nursery bed in 2003. EGV defined as the amount of leaf area and/or dry weight produced early in the season and the EGV-related traits such as length and breadth of the $2^{nd}\;and\;3^{rd}$ leaves showed highly significant positive correlation with the embryo and seed weight. Especially, the genotypic variation in the length of the third leaf was explained over 90% of genotypic variation in the seed weight. Owing to a large effect of seed size on EGV and its related traits, vigor measurements were adjusted based on their linear or exponential relationships with seed weight for excluding the seed weight effect. EGV and its related-traits adjusted for seed weight also showed big variation among genotypes. Increased EGV was genetically correlated with increases in breadth and length of early leaves. The broad-sense heritability for EGV was significantly high(81%), but lower than those of leaf breadth(90% for the $2^{nd}$ leaf and 93% for the $3^{rd}$ leaf) and length(87% for the $2^{nd}$ leaf and 89% for the $3^{rd}$ leaf). Significantly positive genetic correlations were found between EGV and the breadth and length of early leaves. The high heritability of early leaf breadth and length coupled with their strong genetic correlation with EGV indicated that the breadth and length of the $2^{nd}\;and\;3^{rd}$ leaf would be used as good indirect indicators for EGV selection in rice breeding program.

Growth Inhibition against Contaminants in Aseptic Chocolate Milk Using Physicochemical Methods (물리.화학적 처리에 의한 멸균 초콜릿 우유 오염균의 생육억제 효과)

  • Choi, Moon-Kyoung;Yoon, So-Young;Lee, So-Young;Kim, Koth-Bong-Woo-Ri;Lee, Chung-Jo;Jung, Ji-Yeon;Kwak, Ji-Hee;Kim, Min-Ji;Kim, Dong-Hyun;SunWoo, Chan;Lee, Ju-Woon;Byun, Myung-Woo;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.40 no.8
    • /
    • pp.1157-1163
    • /
    • 2011
  • This study was conducted to investigate the cause of microbiological contaminants in aseptic chocolate milk and evaluate the effect of a physicochemical treatment on the growth inhibition of isolated bacterial strains. The bacterium isolated from aseptic chocolate milk was identified as Bacillus lentus and was named B. lentus M1. In the heat and pH treatment, the growth of B. lentus was inhibited at 110$^{\circ}C$ for >15 min and at pH's <5 and >10. An electrolyzed water treatment against B. lentus M1, revealed 5 mm growth past the inhibition zone. The effect of ozone gas on B. lentus M1 growth was evaluated using viable cell counts. When the initial number of B. lentus M1 was $10^2$ and $10^3$ CFU, the bacteria were completely suppressed by ozone gas treatment for 10 and 30 min, respectively. In a microwave treatment, B. lentus M1 was sterilized following microwave treatment for 1 min. As the result of ${\gamma}$-irradiation against B. lentus M1, numbers decreased as the ${\gamma}$-irradiation dosage increased. These results show the growth inhibition effects against contaminants in aseptic chocolate milk using physicochemical treatments.

Evaluation of Image Quality in Micro-CT System Using Constrained Total Variation (TV) Minimization (Micro-CT 시스템에서 제한된 조건의 Total Variation (TV) Minimization을 이용한 영상화질 평가)

  • Jo, Byung-Du;Choi, Jong-Hwa;Kim, Yun-Hwan;Lee, Kyung-Ho;Kim, Dae-Hong;Kim, Hee-Joung
    • Progress in Medical Physics
    • /
    • v.23 no.4
    • /
    • pp.252-260
    • /
    • 2012
  • The reduction of radiation dose from x-ray is a main concern in computed tomography (CT) imaging due to the side-effect of the dose on human body. Recently, the various methods for dose reduction have been studied in CT and one of the method is a iterative reconstruction based on total variation (TV) minimization at few-views data. In this paper, we evaluated the image quality between total variation (TV) minimization algorithm and Feldkam-Davis-kress (FDK) algorithm in micro computed tomography (CT). To evaluate the effect of TV minimization algorithm, we produced a cylindrical phantom including contrast media, water, air inserts. We can acquire maximum 400 projection views per rotation of the x-ray tube and detector. 20, 50, 90, 180 projection data were chosen for evaluating the level of image restoration by TV minimization. The phantom and mouse image reconstructed with FDK algorithm at 400 projection data used as a reference image for comparing with TV minimization and FDK algorithm at few-views. Contrast-to-noise ratio (CNR), Universal quality index (UQI) were used as a image evaluation metric. When projection data are not insufficient, our results show that the image quality of reconstructed with TV minimization is similar to reconstructed image with FDK at 400 view. In the cylindrical phantom study, the CNR of TV image was 5.86, FDK image was 5.65 and FDK-reference was 5.98 at 90-views. The CNR of TV image 0.21 higher than FDK image CNR at 90-views. UQI of TV image was 0.99 and FDK image was 0.81 at 90-views. where, the number of projection is 90, the UQI of TV image 0.18 higher than FDK image at 90-views. In the mouse study UQI of TV image was 0.91, FDK was 0.83 at 90-views. the UQI of TV image 0.08 higher than FDK image at 90-views. In cylindrical phantom image and mouse image study, TV minimization algorithm shows the best performance in artifact reduction and preserving edges at few view data. Therefore, TV minimization can potentially be expected to reduce patient dose in clinics.

Antioxidative Activity and Component Analysis of Cayratia japonica Extract (오렴매 추출물의 항산화 활성, 성분 분석)

  • Yang, Hee-Jung;Kim, Eun-Hee;Park, Soo-Nam
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.34 no.2
    • /
    • pp.117-127
    • /
    • 2008
  • In this study, the antioxidative effects, inhibitory effects on elastase, and components of Cayratia japonica extracts were investigated. The free radical(1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities($FSC_{50}$) of extract/fractions of Cayratia japonica were in the order: 50% ethanol extract(114.3 ${\mu}g/mL$)${\mu}g/mL$)${\mu}g/mL$). Reactive oxygen species(ROS) scavenging activities($OSC_{50}$) of some Cayratia japonica extracts in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activities were deglycosylated flavonoid aglycone fraction($OSC_{50},\;3.30{\mu}g/mL$)<50% ethanol extract(1.21 ${\mu}g/mL$)${\mu}g/mL$). Ethyl acetate fraction showed the most prominent scavenging activity. The protective effects of extract/fractions of Cayratia japonica on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Cayratia japonica extracts suppressed photohemolysis in a concentration dependent manner($1{\sim}25{\mu}g/mL$), particularly deglycosylated flavonoid aglycone fraction exhibited the most prominent celluar protective effect(${\tau}_{50}$, 175.05min at 25 ${\mu}g/mL$). Aglycone fractions obtained from the deglycosylation reaction of ethyl acetate fraction among the Cayratia japonica extracts, showed 2 bands in TLC and 2 peaks in HPLC experiments(360 nm). Two components were identified as luteolin(composition ratio, 47.50%), apigenin(52.50). TLC chromatogram of ethyl acetate fraction of Cayratia japonica extract revealed 3 bands and HPLC chromatogram showed 4 peaks, which were identified as luteolin-7-O-${\beta}$-D-glucopyranoside(composition ratio, 11.14%), apigenin-7-O-${\beta}$-D-glucuronopyranoside(15.38%), luteolin(23.55%) and apigenin(49.92%) in the order of elution time. The inhibitory effect of aglycone fraction on elastase($IC_{50},\;70.5{\mu}g/mL$) was very high. These results indicate that extract/fractions of Cayratia japonica can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. And component analysis of Cayratia japonica extract and antioxidative effects could be applicable to new cosmetics.

Antioxidative Effects of Inula britannica var. chinensis Flower Extracts According to the flowering period and species of Inula britannica var. chinensis (금불초 종(種) 및 개화시기에 따른 금불초 꽃 추출물의 항산화 효능)

  • Kwon, Soon Sik;Jeon, So Ha;Jeon, Ji Min;Cheon, Jong Woo;Park, Soo Nam
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.39 no.3
    • /
    • pp.195-203
    • /
    • 2013
  • In this study, antioxidative effects of the extracts of different species and flowering periods of Inula britannica were investigated. According to the free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity of the extracts, The I. britannica var. chinensis flower extract (500 ${\mu}g/mL$) was measured in a 79.89% free radical scavenging activity, but the flower extracts of similar species (I. britannica var. linariaefolia Regel, I. britannica var. ramosa, I. salicina var. asiatica) did not show any effect on the free radical scavenging activity. The effects of the free radical scavenging activity of I. britannica var. chinensis flower extracts were exhibited in the order of full bloom (93.68%), bud (43.28%), and fallen blossom (14.11%). Next, we established optimum condition of extract solvent, temperature, extraction time. The extract from ethanol at $60^{\circ}C$ showed the most free radical scavenging activity among other conditions and extraction time not relevant in free radical scavenging activity. The protective effects of the extract of I. britannica var. chinensis flower on the photohemolysis of human erythrocytes by using rose bengal were increased in a concentration-dependent manner (5 ~ 50 ${\mu}g/mL$). In particular, the extract in 50 ${\mu}g/mL$ concentration exhibited better protective activity (${\tau}_{50}$ = 116.1 min) than (+)-${\alpha}$-tocopherol (${\tau}_{50}$ = 73.44 min), which is a known lipophilic antioxidant. Principle component of I. britannica var. chinensis flower was identified as quercetin of flavonoids by high-performance liquid chromatography (HPLC). These results indicate that the extract of I. britannica var. chinensis flower can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging free radical and $^1O_2$, and protect cellular membranes against ROS. It is concluded that the antioxidative effects of the extract of I. britannica var. chinensis flower could be applicable to functional cosmetics.

Antioxidative Activities and Antiaging Effects of Geum aleppicum Jacq. Extracts (큰뱀무 추출물의 항산화 활성 및 항노화에 관한 연구)

  • Kim, Min-Ji;Yang, Hyun-Gab;Park, Soo-Nam
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.37 no.2
    • /
    • pp.191-198
    • /
    • 2011
  • In this study, antioxidative effects and inhibitory effects of Geum aleppicum Jacq. extracts on tyrosinase and elastase were investigated. The ethyl acetate fraction of G. aleppicum Jacq. extract ($4.70\;{\mu}g$/mL) showed the most prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of some G. aleppicum Jacq. extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction showed the most prominent ROS scavenging activity ($0.22 \;{\mu}g$/mL). The protective effects of extract/fraction of G. aleppicum Jacq. against the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The G. aleppicum Jacq. extracts suppressed photohemolysis in a concentration dependent manner ($1{\sim}25{\mu}g$/mL), particularly the ethyl acetate fraction exhibited the most prominent celluar protective effect (${\tau}_{50}$, 416.20 min at $10 \;{\mu}g$/mL). The inhibitory effect of G. aleppicum Jacq. extracts on tyrosinase and elastase were investigated to assess their whitening and anti-winkle efficacy. The half maximal inhibitory concentration ($IC_{50}$) of the ethyl acetate fraction on tyrosinase was $95.23\;{\mu}g$/mL. The $IC_{50}$ of 50 % ethanol extract and the ethyl acetate fraction on elastase were $6.27 \;{\mu}g$/mL and $4.31 \;{\mu}g$/mL, respectively. These results indicate that extract/fraction of G. aleppicum Jacq. can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Especially the ethyl acetate fraction of G. aleppicum Jacq. extracts could be applicable to new functional cosmetics for antioxidant, antiaging.

Antioxidative Activities and Antiaging Effects of Geranium nepalense Extracts (현초 추출물의 항산화 활성 및 항노화에 관한 연구)

  • Lee, Keun-Ha;Park, Soo-Nam
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.34 no.1
    • /
    • pp.25-35
    • /
    • 2008
  • In this study, the antioxidative effects and inhibitory effects on elastase and tyrosinase of Geranium nepalense extracts were investigated. The free radical(1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities ($FSC_{50}$) of extract/fractions of Geranium nepalense were in the order: 50% ethanol extract(15.0 ${\mu}g/mL$)${\mu}g/mL$). ${\mu}g/mL$). Reactive oxygen species (ROS) scavenging activities($OSC_{50}$) of some Geranium nepalense extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol-dependent chemiluminescense assay. The order of ROS scavenging activities were 50% ethanol extract($OSC_{50},\;0.23{\mu}g/mL$)${\mu}g/mL$)${\mu}g/mL$). Deglycosylated flavonoid fraction showed the most prominent scavenging activity. The protective effects of extract/fractions of Geranium nepalense on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Geranium nepalense extracts suppressed photohemolysis in a concentration dependent manner, particularly deglycosylated flavonoid fraction exhibited the most prominent celluar protective effect (${\tau}_{50}$, 676.7 min at 50 ${\mu}g/mL$). The inhibitory effect of aglycone fraction on tyrosinase($IC_{50}$, 70.0 ${\mu}g/mL$) and elastase ($IC_{50}$, 19.9 ${\mu}g/mL$) was very high. Aglycone fractions obtained from the deglycosylation reaction of ethyl-acetate fraction among the Geranium nepalense extracts, showed 2 bands in TLC and 2 peaks in HPLC experiments (370 nm). Two components were identified as quercetin(composition ratio, 15.3%), kaempferol(82.8%). These results indicate that extract/fractions of Geranium nepalense can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. And component analysis of Geranium nepalense extract and inhibitory activity on elastase of the aglycone fraction could be applicable to new functional cosmetics for smoothing wrinkles.

Effect of Shading Methods on Growth and Fruit Quality of Paprika in Summer Season (파프리카 여름재배시 차광방법이 생육과 과실특성에 미치는 영향)

  • Ha, Jun Bong;Lim, Chae Shin;Kang, Hyo Yong;Kang, Yang Su;Hwang, Seung Jae;Mun, Hyung Su;An, Chul Geon
    • Journal of Bio-Environment Control
    • /
    • v.21 no.4
    • /
    • pp.419-427
    • /
    • 2012
  • This study was carried out to investigate the effect of two shading methods, shading agent spray on the glasshouse and internal shading screen treatment, on the growth and fruit quality of paprika (Capsicum annuum L. 'Cupra' and 'Coletti') in summer season cultivation. In the shading agent treatment, a commercial shading agent diluted with water at a ratio of 1 : 4 was sprayed on the roof of a glasshouse. In the internal shading screen treatment, a 10~20% shaded screen was used during the day time when the sun radiation was greater than $700W{\cdot}m^{-2}$. Compared to the unshaded control, photosynthetic photon flux density (PPFD) decreased in the greenhouse in the shading agent (SA) and shading screen (SS) treatments by 20% and 30%, respectively. Lower air temperatures and higher relative humidities were observed in the SA than in both the control and the SS treatment. Time to reach the break point of humidity deficit $8g{\cdot}m^{-3}$ was 2 hours late in the SA than in both the control and the SS treatment. Compared to control, both the SA and the SS treatments showed lower instantaneous temperatures of leaf, fruit, and flower by $2^{\circ}C$, $5^{\circ}C$ and $3^{\circ}C$, respectively. There were no differences in number of branches, stem diameter, and leaf size among treatments although both shading treatments promoted plant height in both cultivars. Botrytis infection ratio declined with the SA treatment by 14.7% in 'Cupra' and 22.1% in 'Coletti' as compared to that in the control. Shading increased fruit size in both cultivars, whereas no differences were observed in the number of locules and thickness of fruit tissue among treatments. Shading treatment increased mean fruit weight by a range of 10 to 15 g per fruit, while it decreased soluble solids contents as compared to that in the control. Similar Hunter values were observed among treatments, while fruit firmness increased slightly in shading treatments. Compared to the control, shading treatments improved marketable fruits by 11.7~22.6% and increased the number of fruits per plant by 4~9.2 in both 'Cupra' and 'Coletti'. The results of this study indicate that shading agent application on the roof of glasshouse would be one of the most effective options to reduce heat stress imposed on the paprika crop in summer cultivation, resulting in improved crop growth and fruit yield.