• Elastomers and Composites
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• 제48권4호
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• pp.263-268
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• 2013

본 연구에서는 다리 구조의 메탈로센 촉매인 rac-$Et[Ind]_2ZrCl_2$를 이용하여 새로운 구조의 poly(ethylene-ter-1-hexene-ter-divinylbenzene) 삼원공중합체를 제조하였다. 공단량체인 1-hexene이 중합에 미치는 효과에 관한 연구를 수행하였다. 여러 가지 중합 조건의 영향을 살펴보았는데 공촉매/촉매 몰비가 3,000일 때, 촉매활성도는 8,000이 넘는 매우 높은 수준의 활성도를 보여주었다. 또한, 중합 시간에 따라 중량 평균 분자량이 일정 수준까지 증가하는 경향을 나타내었고, 중합시간이 50분일 때는 무정형 상태에 가까워지는 것을 확인하였다. 삼원공중합체의 중량 평균 분자량은 110,000-200,000, 밀도는 $0.85-0.89g/cm^3$ 수준이었다. 또한, 삼원 공중합체의 열적 성질과 구조를 확인하였다.

• Korean Chemical Engineering Research
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• 제58권1호
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• pp.122-126
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• 2020

본 연구에서는 폴리디메틸실록산(PDMS)과 모세관-미세몰딩(MIMIC) 기술을 활용하여 마이크로패턴 채널 시스템을 제작하고, 단일 세포 수준에서 극성화 패턴으로 형성되는 분자 신호를 고해상도 세포 이미징을 통해 분석하였다. 이 과정에서 혈소판유래성장인자(PDGF)가 처리된 세포에서는 세포 이동에 중요한 세 종류의 신호인 포스포이노시티드 3-인산화효소(PI3K), Rac 및 액틴(Actin) 신호가 선두(front)영역에서 후미(rear)영역에 비해 강하게 활성화 하는 데 반해, 마이오신 경쇄(MLC) 신호는 비특이적 경향성을 보여주었다. 본 연구 결과는 향후 마이크로패턴의 미세환경에서 세포 극성화 신호와 세포 이동과의 상관 관계를 연구하는 데 중요한 도움이 될 것으로 사료된다.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2002년도 동계연구발표회 논문개요집
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• pp.130-131
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• 2002

Terfenol-D는 상온에서 변형이 가장 큰 재료로 알려져 있고, 자기역학적 결합계수 k$_{33}$=0.75로 매우 높기 때문에 전기에너지를 역학적 에너지로 효율적으로 변환시킬 수 있다[1]. 단점으로는 압축강도는 700 ㎫로 높으나, 인장강도 30 ㎫로 매우 낮아서 재료가 brittle하여 장치 설계 시 세심한 주의가 필요하며, 재료의 상대투자율이 3~10정도로 낮기 때문에 자기폐회로를 구성하는데 어려움이 있다[2-4]. (중략)략)

• BMB Reports
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• 제51권7호
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• pp.356-361
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• 2018

Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, mediates interactions between actin filaments and cytoplasmic targets. However, the role of ABLIM1 in osteoclast and bone metabolism has not been reported. In the present study, we investigated the role of ABLIM1 in the receptor activator of $NF-{\kappa}B$ ligand (RANKL)-mediated osteoclastogenesis. ABLIM1 expression was induced by RANKL treatment and knockdown of ABLIM1 by retrovirus infection containing Ablim1-specific short hairpin RNA (shAblim1) decreased mature osteoclast formation and bone resorption activity in a RANKL-dose dependent manner. Coincident with the downregulated expression of osteoclast differentiation marker genes, the expression levels of c-Fos and the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), critical transcription factors of osteoclastogenesis, were also decreased in shAblim1-infected osteoclasts during RANKL-mediated osteoclast differentiation. In addition, the motility of preosteoclast was reduced by ABLIM1 knockdown via modulation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt/Rac1 signaling pathway, suggesting another regulatory mechanism of ABLIM1 in osteoclast formation. These data demonstrated that ABLIM1 is a positive regulator of RANKL-mediated osteoclast formation via the modulation of the differentiation and PI3K/Akt/Rac1-dependent motility.

• IMMUNE NETWORK
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• 제15권2호
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• pp.100-109
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• 2015

Controlling balance between T-helper type 1 (Th1) and T-helper type 2 (Th2) plays a pivotal role in maintaining the biological rhythm of Th1/Th2 and circumventing diseases caused by Th1/Th2 imbalance. Interleukin 4 (IL-4) is a Th2-type cytokine and often associated with hypersensitivity-related diseases such as atopic dermatitis and allergies when overexpressed. In this study, we have tried to elucidate the function of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) as an essential modulator of Th1/Th2 balance. EC-18 has showed an inhibitory effect on the production of IL-4 in a dose-dependent manner. RT-PCR analysis has proved EC-18 affect the transcription of IL-4. By analyzing the phosphorylation status of Signal transducer and activator of transcription 6 (STAT6), which is a transcriptional activator of IL-4 expression, we discovered that EC-18 induced the decrease of STAT6 activity in several stimulated cell lines, which was also showed in STAT6 reporter analysis. Co-treatment of EC-18 significantly weakened atopy-like phenotypes in mice treated with an allergen. Collectively, our results suggest that EC-18 is a potent Th2 modulating factor by regulating the transcription of IL-4 via STAT6 modulation, and could be developed for immune-modulatory therapeutics.

• Nutrition Research and Practice
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• 제14권2호
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• pp.127-133
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• 2020

BACKGROUND/OBJECTIVES: Non-small cell lung cancer is mostly recognized among other types of lung cancer with a poor prognosis by cause of chemotherapeutic resistance and increased metastasis. Luteolin has been found to decrease cell metastasis. However, its underlying mechanisms remain unresolved. The objective of this study was to examine the effect (and its mechanism) of luteolin on the migration and invasion of human non-small cell lung cancer A549 cells. MATERIALS/METHODS: Cell viability was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Wound healing and transwell assays were evaluated to assess migration and invasion, respectively. Western blot analysis and immunofluorescence were further performed to investigate the role of luteolin and its mechanisms of action. RESULTS: Administration with up to 40 μM luteolin showed no cytotoxic activity on lung cancer A549 cells or non-cancer MRC-5 cells. Additionally, luteolin at 20-40 μM significantly suppressed A549 cells' migration, invasion, and the formation of filopodia in a concentration-dependent manner at 24 h. This is similar with western blot analysis, which revealed diminished the phosphorylated focal adhesion kinase (pFAK), phosphorylated non-receptor tyrosine kinase (pSrc), Ras-related C3 botulinum toxin substrate 1 (Rac1), cell division control protein 42 (Cdc42), and Ras homolog gene family member A (RhoA) expression levels. CONCLUSIONS: Overall, our data indicate that luteolin plays a role in controlling lung cancer cells' migration and invasion via Src/FAK and its downstream Rac1, Cdc42, and RhoA pathways. Luteolin might be considered a promising candidate for suppressing invasion and metastasis of lung cancer cells.

• Steel and Composite Structures
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• 제44권3호
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• pp.309-324
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• 2022

The post-fire elastic stiffness and performance of concrete-filled steel tube (CFST) columns containing recycled aggregate concrete (RAC) has rarely been addressed, particularly in terms of material properties. This study was conducted with the aim of assessing the modulus of elasticity of recycled aggregate concrete-filled steel tube (RACFST) stub columns following thermal loading. The test data were employed to model and assess the elastic modulus of circular RACFST stub columns subjected to axial loading after exposure to elevated temperatures. The length/diameter ratio of the specimens was less than three to prevent the sensitivity of overall buckling for the stub columns. The gene expression programming (GEP) method was employed for the model development. The GEP model was derived based on a comprehensive experimental database of heated and non-heated RACFST stub columns that have been properly gathered from the open literature. In this study, by using specifications of 149 specimens, the variables were the steel section ratio, applied temperature, yielding strength of steel, compressive strength of plain concrete, and elastic modulus of steel tube and concrete core (RAC). Moreover, parametric and sensitivity analyses were also performed to determine the contribution of different effective parameters to the post-fire elastic modulus. Additionally, comparisons and verification of the effectiveness of the proposed model were made between the values obtained from the GEP model and the formulas proposed by different researchers. Through the analyses and comparisons of the developed model against formulas available in the literature, the acceptable accuracy of the model for predicting the post-fire modulus of elasticity of circular RACFST stub columns was seen.

• Journal of Periodontal and Implant Science
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• 제36권4호
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• pp.863-878
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• 2006

Porphyromonas gingivalis는 치주질환을 야기하는 독성세균으로서, 구강상피세포에 p. gingivalis가 감염되었을 때, 세포형태에 변화를 초래함으로 인해 방어기작이 작동하게 된다. 치주질환과 관련되어 생성된 활성 산소종의 소거에 관여하는 항산화성분은 p. gingivalis 이 감염된 구강상피세포에서 그 분포와 발현수준이 달라지리라 예상된다. 따라서 이번 연구에서는 구강상피세포(KB 세포)에 p. gingivalis가 감염되었을 때 야기되는 활성산소종과 이를 소거하는 역할을 하는 항산화단백들의 역할들을 규명하고자 하였다. 활성산소종 형성을 조절하는 NADPH oxidase 중 NOX4와 Rac1 전사체는 구강상피세포에서 p. gingivalis세균에 의해 증가하였으며 $gp91^{phox}$, Rac2, $p47^{phox}$와 $p67^{phox}$는 세균에 의한 변화가 관찰되지 않았다. 반면에 $p40^{phox}$ 전사체는 감소하는 경향을 보였다. NOX1 전사체는 p. gingivalis 처리 30분 후 감소하였다가 60분 후에는 다시 증가하는 양상을 보였다. 같은 시간에 NOX 활성화 단백인 NOXA1은 감소하고, NOX 구성단백질인 NOXO1은 증가하는 경향을 보였다. p. gingivalis가 감염된 구강상피세포를 방어하는 항산화단백 발현수준을 평가한 결과, SOD1, 2, 3 모두 p. gingivalis 처리시간에 따라 증가하는 양상을 보였다. GPx 발현 양상도 SOD와 유사하게 나타났다. $H_2O_2$를 소거하는 Prx는 감염된 KB 세포에서 Prx4와 Prx5가 4-6배 증가하는 것을 알 수 있었다. 반면 endocytosis 과정 중 $H_2O_2$ 생산은 변화되지 않았다. 이번 연구의 결과, p. gingivalis의 감염은 KB 세포의 NOX4와 Rac1의 NADPH oxidase 발현을 증가시켰으며, NOX1은 NOXA1과 NOXO1의 조절에 의해 영향을 받음을 알 수 있었다. 또한 항산화기작으로는 SOD, GPx, Prx가 증가하였는데, 이것은 Prx4와 Prx5가 중요한 역할을 할 것을 시사하였다.

• 지질공학
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• 제13권3호
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• pp.345-358
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• 2003

근래에 와서 천연골재자원의 보호와 건설 폐기물의 재활용의 측면에서 폐 콘크리트에서 분리한 재생골재의 재활용이 모색되어 왔으나, 재생골재는 주로 콘크리트 도로기층용과 구조물 됫채움재와 같은 저급재료로 사용되고 있다. 본 연구는 재생골재의 콘크리트 용 골재로의 효율적인 재활용 가능성을 위하여, 재생골재의 구성광물, 화학조성 및 공학적 물성 특성 파악하고 재생콘크리트(RCA)의 시공성과 역학적 특성에 대한 연구를 수행하였다. 재생골재에 잔존하는 폐 콘크리트의 모르타르 및 시멘트 페이스트는 골재의 기초 물성 특성, 재생콘크리트의 시공성 및 굳은 콘크리트의 공학적 특성에 주요한 영항을 미치는 것으로 나타났다. 그러나, 적절한 양의 재생굵은골재를 대체한 재생콘크리트는 압축강도와 동결융해 내구성에서 보통 콘크리트의 기준에 근접하는 것으로 나타났다 따라서, 재생골재의 제조과정에서 폐 콘크리트의 모르타르 및 시멘트 페이스트의 제거가 효율적으로 이루어지고 적절한 대체율이 적용되면, 재생골재는 콘크리트용 골재로서 효율적인 사용이 확대 될 수 있을 것으로 사료된다.

• Advances in concrete construction
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• 제9권2호
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• pp.195-205
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• 2020

In this study, effect of recycled aggregates and polypropylene fibers on the response of conventionally reinforced concrete element subjected to tensile loading in terms of tension stiffening and strain development was experimentally investigated. For this purpose, concrete prisms of 100 × 100 mm cross section and 500 mm length having one central deformed steel re-bar were cast using fibrous and non-fibrous Recycled Aggregate Concrete (RAC) with varying percentages of recycled aggregates (0%, 25%, 50%, 75% and 100%) and tested under uniaxial tensile load. For all fibrous RAC mixes, polypropylene fibers were used at constant dosage of 3.15 kg/㎥. Effect of recycled aggregates and fibers on the compressive strength of concrete was also explored in this study. Through studying tensile load versus global axial deformation of composite and strain development in concrete and steel, it was found that replacement of natural aggregates with recycled aggregates in concrete negatively affected the cracking load, tension stiffening and strain development, and this negative effect was observed to be increased with increasing contents of recycled aggregates in concrete. The results of this study showed that it was possible to minimize the negative effect of recycled aggregates in concrete by the addition of polypropylene fibers. Reinforced concrete element constructed using concrete containing 50% recycled aggregates and polypropylene fibers exhibited cracking behavior, tension stiffening and strain development response almost similar to that of concrete element constructed using natural aggregate concrete without fiber.