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1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) Modulates Th2 Immunity through Attenuation of IL-4 Expression

  • Yoon, Sun Young (ENZYCHEM Lifesciences) ;
  • Kang, Ho Bum (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ko, Young-Eun (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Shin, Su-Hyun (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Jun (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Sohn, Ki-Young (ENZYCHEM Lifesciences) ;
  • Han, Yong-Hae (ENZYCHEM Lifesciences) ;
  • Chong, Saeho (ENZYCHEM Lifesciences) ;
  • Kim, Jae Wha (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2015.02.03
  • Accepted : 2015.03.30
  • Published : 2015.04.30
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Abstract

Controlling balance between T-helper type 1 (Th1) and T-helper type 2 (Th2) plays a pivotal role in maintaining the biological rhythm of Th1/Th2 and circumventing diseases caused by Th1/Th2 imbalance. Interleukin 4 (IL-4) is a Th2-type cytokine and often associated with hypersensitivity-related diseases such as atopic dermatitis and allergies when overexpressed. In this study, we have tried to elucidate the function of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) as an essential modulator of Th1/Th2 balance. EC-18 has showed an inhibitory effect on the production of IL-4 in a dose-dependent manner. RT-PCR analysis has proved EC-18 affect the transcription of IL-4. By analyzing the phosphorylation status of Signal transducer and activator of transcription 6 (STAT6), which is a transcriptional activator of IL-4 expression, we discovered that EC-18 induced the decrease of STAT6 activity in several stimulated cell lines, which was also showed in STAT6 reporter analysis. Co-treatment of EC-18 significantly weakened atopy-like phenotypes in mice treated with an allergen. Collectively, our results suggest that EC-18 is a potent Th2 modulating factor by regulating the transcription of IL-4 via STAT6 modulation, and could be developed for immune-modulatory therapeutics.

Keywords

References

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