• 해양환경안전학회지
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• 제20권6호
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• pp.685-691
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• 2014

해상에서 발생하는 위기의 확률적인 평가결과는 확률(P=0.0~1.0)로 나타난다. 이러한 확률적인 위기평가결과는 위험한 정도를 쉽게 알기 위하여 일반적으로 평가의 기준이 되는 위기허용수준(Risk Acceptance Criteria, RAC)을 이용하여 지수(index)로 나타낸다. 현재 RAC은 3단계, 5단계, 7단계 등으로 구분하는데, 구분한 단계 수가 위기평가에 적합한지를 평가할 필요가 있음에도 불구하고 관련 연구가 전무한 실정이다. 본 연구에서는 RAC의 단계 구분 수에 따른 민감도(Sensitivity)와 민감도의 분포특성을 이용하여 최적의 RAC 구분 단계 수를 정할 수 있는 평가기법을 제안하였다. 사전 연구결과로부터 획득한 확률적인 위기평가 데이터에 대해서 제안한 기법을 적용한 결과, 민감도가 10배 이내로 형성되는 최적의 RAC 구분 단계 수를 결정할 수 있었고, 이를 통해서 제안한 방법의 유효성을 확인하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제27권3호
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• pp.257-265
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• 2023

Kidney ischemia/reperfusion (I/R) injury, a common cause of acute kidney injury (AKI), is associated with the migration of inflammatory cells into the kidney. Ras-related C3 botulinum toxin substrate 1 (Rac1), a member of the Rho family of small GTPase, plays an important role in inflammatory cell migration by cytoskeleton rearrangement. Here, we investigated the role of Rac1 on kidney I/R injury and macrophage migration. Male mice were subjected to either 25 min of bilateral ischemia followed by reperfusion (I/R) or a sham operation. Some mice were administrated with either NSC23766, an inhibitor of Rac1, or 0.9% NaCl (vehicle). Kidney damage and Rac1 activity and expression were measured. The migration and lamellipodia formation of RAW264.7 cells, mouse monocyte/macrophage, induced by monocyte chemoattractant protein-1 (MCP-1, a chemokine) were determined using transwell migration assay and phalloidin staining, respectively. In sham-operated kidneys, Rac1 was expressed in tubular cells and interstitial cells. In I/R-injured kidneys, Rac1 expression was decreased in tubule cells in correlation with the damage of tubular cells, whereas Rac1 expression increased in the interstitium in correlation with an increased population of F4/80 cells, monocytes/macrophages. I/R increased Rac1 activity without changing total Rac1 expression in the whole kidney lysates. NSC23766 administration blocked Rac1 activation and protected the kidney against I/R-induced kidney damage and interstitial F4/80 cell increase. NSC23766 suppressed monocyte MCP-1-induced lamellipodia and filopodia formation and migration of RAW 264.7 cells. These results indicate Rac1 inhibition protects the kidney against I/R via inhibition of monocytes/macrophages migration into the kidney.

• Structural Engineering and Mechanics
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• 제64권4호
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• pp.461-472
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• 2017

The dynamic tests of recycled aggregate concrete (RAC) are carried out, the rate-dependent mechanical models of RAC are proposed. The dynamic mechanical behaviors of RAC frame structure are investigated by adopting the numerical simulation method of the finite element. It is indicated that the lateral stiffness and the hysteresis loops of RAC frame structure obtained from the numerical simulation agree well with the test results, more so for the numerical simulation which is considered the strain rate effect than for the numerical simulation with strain rate excluded. The natural vibration frequency and the lateral stiffness increase with the increase of the strain rate. The dynamic model of the lateral stiffness is proposed, which is reasonably applied to describe the effect of the strain rate on the lateral stiffness of RAC frame structure. The effect of the strain rate on the structural deformation and capacity of RAC is analyzed. The analyses show that the inter-story drift decreases with the increase of the strain rate. However, with the increasing strain rate, the structural capacity increases. The dynamic models of the base shear coefficient and the overturning moment of RAC frame structure are developed. The dynamic models are important and can be used to evaluate the strength deterioration of RAC structure under dynamic loading.

• BMB Reports
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• 제46권12호
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• pp.617-622
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• 2013

Rac1 plays a key role in neurite outgrowth via reorganization of the actin cytoskeleton. The molecular mechanisms underlying Rac1-mediated actin dynamics in the cytosol and plasma membrane have been intensively studied, but the nuclear function of Rac1 in neurite outgrowth has not yet been addressed. Using subcellular fractionation and immunocytochemistry, we sought to explore the role of nuclear Rac1 in neurite outgrowth. bFGF, a strong agonist for neurite outgrowth in PC12 cells, stimulated the nuclear accumulation of an active form of Rac1. Rac1-PBR (Q) mutant, in which six basic residues in the polybasic region at the C-terminus were replaced by glutamine, didn't accumulate in the nucleus. In comparison with control cells, cells expressing this mutant form of Rac1 displayed a marked defect in extending neurites that was concomitant with reduced expression of MAP2 and MEK-1. These results suggest that Rac1 translocation to the nucleus functionally correlates with bFGF-induced neurite outgrowth.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.3061-3065
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• 2015

Background: Rac3, a member of the Rac family of small guanosine triphosphatases (GTPases), regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. Overexpression of Rac3 has been reported in several human cancers. However, the role of Rac3 in lung cancer (LC) has not been determined in detail. The purpose of this study was to investigate the effect of silencing of Rac3 expression in human LC cells and the consequences for cell survival. Materials and Methods: Lentivirus small hairpin RNA (shRNA) interference techniques were utilized to knock down the Rac3 gene. Gene and protein expression was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. LC cell apoptosis was examined by annexin V-APC /propidium iodide staining. Results: Efficient silencing of Rac3 strongly inhibited A549 cell proliferation and colony formation ability, and significantly decreased tumor growth. Moreover, flow cytometry analysis showed that knockdown of Rac3 led to G2/M phase cell cycle arrest as well as an excess accumulation of cells in the G1 and S phase. Conclusions: Thus, functional analysis using shRNAs revealed a critical role for Rac3 in the tumor growth of LC cells. shRNA silencing of Rac3 could provide an effective strategy to treat LC.

• Molecules and Cells
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• 제25권1호
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• pp.131-137
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• 2008

Crk-associated substrate (CAS) is a focal adhesion protein that is involved in integrin signaling and cell migration. CAS deficiency reduces the migration and spreading of cells, both of which are processes mediated by Rac activation. We examined the functions of v-Crk, the oncogene product of the CT10 virus p47gag-crk, which affects cell migration and spreading, membrane ruffling, and Rac activation in CAS-deficient mouse embryonic fibroblasts (CAS-/- MEFs). CAS-/- MEFs showed less spreading than did CAS+/+ MEFs, but spreading was recovered in mutant cells that expressed v-Crk (CAS-/-v-Crk MEF). We observed that the reduction in spreading was linked to the formation of membrane ruffles, which were accompanied by Rac activation. In CAS-/- MEFs, Rac activity was significantly reduced, and Rac was not localized to the membrane. In contrast, Rac was active and localized to the membrane in CAS-/-v-Crk MEFs. Lamellipodia protrusion and ruffle retraction velocities were both reduced in CAS-/- MEFs, but not in CAS-/-v-Crk MEFs. We also found that microinjection of anti-gag antibodies inhibited the migration of CAS-/-v-Crk MEFs. These findings indicate that v-Crk controls cell migration and membrane dynamics by activating Rac in CAS-deficient MEFs.

• 한국해양생명과학회지
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• 제2권2호
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• pp.70-74
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• 2017

IL-1RAcP는 일명 interleukin-1 receptor accessory protein이라 칭하며 interleukin-1 염증성 사이토카인과 interleukin-1 receptor I (IL-1RI) 결합체와 복합체를 형성하여 작용한다. IL-1RAcP는 면역반응, 스트레스 및 세포사멸과 관련이 있다. 본 연구의 목적은 참돔(Pagrus major)을 저수온(8℃, 33 psu) 및 저염분(20℃, 10 psu) 상태에 노출시킨 후, IL-1RAcP 유전자의 발현을 관찰하는 것이다. 연구결과, IL-1RAcP 유전자의 발현은 저수온(8℃, 33 psu) 및 저염분(20℃, 10 psu) 상태에서 유의적으로 증가하는 것으로 나타났다. 이 연구결과로서 IL-1RAcP 유전자는 저수온 및 저염분 등의 환경 스트레스에 대한 생체지표유전자로서 역할을 한다고 제의한다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.3092-3097
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• 2007

Although noise of a RAC can be reduced effectively by decreasing RPM, condensation problems can occur to reduce reliability of the RAC for low RPM. Thus, this research has been performed to propose a design guideline of the RAC for low-noise RPM with high reliability. The internal and external flows of the RAC have been visualized and analyzed by a PIV technique to solve the condensation problem at an outlet and impeller. Then, the design guideline has been proposed by the analyzed results and confirmed by wind-tunnel and noise tests to reduce the condensation problem. Finally the shapes of the outlet with reduced condensation problem and the impeller with low noise have been obtained in this study.

• 한국정보과학회논문지:시스템및이론
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• 제30권9호
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• pp.461-476
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• 2003

메모리 병목현상의 완화와 구현상의 용이함으로 인해 NUMA 시스템이 지난 수년 동안 전형적인 다중 프로세서 시스템으로 자리를 잡아 왔다. 하지만 NUMA 시스템은 그 구조의 특성상 원격 메모리로의 접근 비율이 커질수록 응답 속도의 지연이 심화되므로, NUMA 시스템의 구현에 있어서 원격 캐쉬의 효율적인 설계를 요구한다. 본 논문에서는 보다 효율적인 원격 캐쉬의 설계를 목표로 하여, 원격 캐쉬 상에서 실제 응용 프로그램의 공유 단위(Granularity of Sharing)의 패턴을 분석하여 원격 캐쉬의 라인 사이즈를 실행 시간에 가상적으로 변화시킬 수 있는“다중 그레인 원격 캐쉬”방식을 제안한다. 그리고 이를 MINT를 통해 모델링한 후 시뮬레이션을 수행하고 그 결과를 분석한다. 시뮬레이션에서는 먼저 Profile-Based 방식을 이용하여 각 응용 프로그램별 최적의 원격 캐쉬 라인 사이즈를 찾아내고, 이를 이용하여 기존의 일반적인 NUMA 시스템에서의 원격 캐쉬와 본 논문에서 제안한 다중 그레인 원격 캐쉬와의 상호 비교를 통해 성능상의 차이점을 비교, 분석한다. 그 후 다중 그레인 원격 캐쉬가 시스템과 응용 프로그램간의 다양한 관계 속에서도 항상 최악의 경우를 피하면서 최적의 경우와 유사한 결과를 가짐을 보인다.

• International Journal of Concrete Structures and Materials
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• 제10권3호
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• pp.307-323
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• 2016

The nonlinear behaviors of recycled aggregate concrete (RAC) frame structure are investigated by numerical simulation method with 3-D finite fiber elements. The dynamic characteristics and the seismic performance of the RAC frame structure are analyzed and validated with the shaking table test results. Specifically, the natural frequency and the typical responses (e.g., storey deformation, capacity curve, etc.) from Model 1 (exclusion of strain rate effect) and Model 2 (inclusion of strain rate effect) are analyzed and compared. It is revealed that Model 2 is more likely to provide a better match between the numerical simulation and the shaking table test as key attributes of seismic behaviors of the frame structure are captured by this model. For the purpose to examine how seismic behaviors of the RAC frame structure vary under different strain rates in a real seismic situation, a numerical simulation is performed by varying the strain rate. The storey displacement response and the base shear for the RAC frame structure under different strain rates are investigated and analyzed. It is implied that the structural behavior of the RAC frame structure is significantly influenced by the strain rate effect. On one hand, the storey displacements vary slightly in the trend of decreasing with the increasing strain rate. On the other hand, the base shear of the RAC frame structure under dynamic loading conditions increases with gradually increasing amplitude of the strain rate.