• 한국어병학회지
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• 제19권2호
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• pp.183-188
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• 2006

In fish vaccinology, the secondary antibodies against fish immunoglobulins (Igs) are necessary to measure specific humoral immune responses in immunized fish. In the present study, polyclonal antiserum against olive flounder (Paralichthys olivaceus) IgM heavy chain was generated by intramuscular immunization of rabbit with Escherichia coli/eukaryotic shuttle vector containing open reading frame (ORF) of olive flounder IgM heavy chain. Western blot analysis demonstrated the specific activity of the rabbit antiserum with reduced olive flounder serum H chain at dilutions up to 1:1000. Titer of immunized rabbit serum against olive flounder serum was significantly higher than that of pre-immunized rabbit serum when determined by ELISA.

• 수산해양기술연구
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• 제37권3호
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• pp.223-231
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• 2001

제주도 연안 정치망의 어획성능을 향상시키기 위한 어구구조개량과 조업방식의 생력화에 대한 기초자료를 제공할 목적으로 현재 제주도 연안 정치망 어장에 부설하여 조업을 행하고 있는 각망어구와 낙망어구의 어획성능을 비교하기 위해서 이들 실물어구를 1/30로 축소하고 제작한 모형어구를 이용하여 수조실험을 실시하고 고등어어군, 전갱이 어군, 독가시치 어군의 입·출망 행동을 관찰하고 해석하였는데, 그 결과는 다음과 같다. 1. 어군의 입망율은 경과시간 60초일 때 각망어구에서는 고등어 50%, 전갱이 18%, 독가시치 28%였고, 낙망 어구에서는 고등어 70%, 전갱이 60%, 독가시치 30%였다. 2. 어군의 출망율은 경과시간 60초일 때 각망어구에서는 고등어 70%, 전갱이 40%, 독가시치 24%였고, 낙망어구에서는 고등어, 전갱이가 각각 0%, 독가시치가 3%였다. 3. 어군의 잔존율은 경과시간 60초일 때 각망어구 에서는 고등어 30%, 전갱이 60%, 독가시치 76%였고, 낙망어구에서는 고등어, 전갱이가 각각 100%, 독가시치가 97%였다.

• Environmental Analysis Health and Toxicology
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• 제12권3_4호
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• pp.15-22
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• 1997

This experiment was performed to examine the immuno-reactive characteristics of fish metal-binding protein, metallothionein (MT), and gain the practical understandings for the proposed use of fish MT as a biomarker. Liver MT induced by Cd in the silver carp was seperated and purified by gel filtration chromatography and ion exchange chromatography. The immuno-reactivity of fish MT was examined with 3 rabbit antisera. Fish MT showed little reactivity with rabbit anti-rat MT antiserum and a weak reactivity with anti-MT peptide antiserum while showed a strong reactivity with rabbit anti-fish MT antiserum. The time-course change of liver MT in the silver carp, after waterborne exposure to 1 ppm of Cd, was checked by Cd-hem method and established competitive ELISA. In both cases, the induction of liver MT showed a good increasing relationship with the exposure days. The results indicate that the fish MT can be developed as a useful biomonitoring means in the toxicological study and for the evaluation of water pollution.

• Environmental Analysis Health and Toxicology
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• 제11권1_2호
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• pp.11-17
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• 1996

The purpose of this experiment is to estabilish the sensitive and specific ELISA (enzyme linked immunosorbent assay) system for the detection of fish metallothionein (MT). Silver carp were injected with CA of 1-8mg/kg body wt. 4 times during 10 days. Silver carp was very tolerant species to CA. Cd induced MT in liver was seperated and purified by gel filtration chromatography and ion exchange chromatography and identified by spectrophotometry, native gel electrophoresis and western blot analysis. The rabbit antiserum was produced by immunizing rabbit with lyophilized MT, and the competitive ELISA system was estabilished for the detection of fish MT. In the present ELISA system, the detection limit was about 33 ng/ml. When this ELISA system was employed to determine the MT level in the supernatant sample of fish liver homogenate, the reaction curve showed a good parallel corelationship with the calibration curve over a certain dilution range. The results indicate that the competitive ELISA can be a useful tool for the detection of fish MT in the toxicological study and the evaluation of water pollution.

• 수산해양기술연구
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• 제55권4호
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• pp.349-355
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• 2019

Reproductive ecology of rabbit fish, Siganus fuscescens were investigated from samples collected monthly in the coastal waters off Jeju Island of Korea from February to December 2014. In male and female of S. fuscescens, the values of GSI were reached high values between July to August in which the water temperature was high, and then decreased until December. The occurrence of individuals which have mature eggs in ovary were higher from July to August. Therefore, a main spawning season was estimated from July to August, with the highest peak in July. Throughout histological observation of the ovary in the spawning period, S. fuscescens belong to an group-synchronous and has a one spawning season a year. Fecundity of ovary ranged from 380,000 to 2,600,000 during the spawning period. Size at 50% sexual maturity, determined from logistic equation, were 25.1 cm TL which is over two years.

• 수산해양기술연구
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• 제50권2호
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• pp.169-175
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• 2014

The age and growth of rabbit fish, Siganus fuscescens were investigated from samples monthly collected in the coast of Jeju island, Korea from February to December 2012. Ages were determined from annuli in otoliths, and annuli were formed in one or two months immediately after spawning once a year. Also, main spawning period was estimated between July and August, thus rings were considered as annual marks. The von Bertalanffy growth equations estimated from a non-linear regression method were $L_t=37.24(1-e^{-0.33(t+1.24)})$ ($R^2=0.92$) for female and $L_t=31.44(1-e^{-0.45(t+1.23)})$ ($R^2=0.90$) for male, and the growth between female and male was different.

• 수산해양기술연구
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• 제37권3호
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• pp.232-239
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• 2001

제주도 연안 정치망의 어획성능을 향상시키기 위한 어구구조개량과 조업방식의 생력화에 대한 기초자료를 제공할 목적으로 현재 제주도 연안정치망 어장에 부설하여 조업을 행하고 있는 각망어구와 낙망어구의 어획성능을 비교하기 위해서 두 어장의 3년(1997~1999년)간 어획량을 분석하였는데, 그 결과는 다음과 같다. 1. 1997~1999년까지의 3년간의 총어획량은 각망어구가 18.7톤 낙망어구가 36.5톤으로 낙망구의 어획량이 2배 정도 많았다. 2. 1997~1999년까지의 오징어, 전갱이, 독가시와 방어의 어획량은 전체적으로 각망어구보다 낙망어구의 어척량이 1.8배 정도 많았다. 3. CPUE는 각망어구에서는 전갱이가 10.01k 오징어가 20.5kg, 독가시치가 18.0kg 방어가 2.2kg이며 낙망어구에서는 전갱이가 57.5kg, 오징어가 30.0kg, 독가시치가 25.0kg, 방어가 4.7kg였는데 전반적으로 낙망어구의 CPUE가 높게 나타났다. 4. 유어율은 각망어구에서는 전갱이가 64%, 오징어가 79%, 독가시치가 39% 방어가 14%이고 낙망어구에서는 전갱이가 18%, 오징어가 85%, 독가시치가 40%, 방어가 14%로 전체적으로는 각망어구의 유어율이 높게 나타났으나 여기서의 어획량은 낙망어구가 높게 나타났다.

• 한국수산과학회지
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• 제29권5호
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• pp.620-628
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• 1996

여러 다른 S. aureus strains및 토끼항체를 사용하여 항체감작을 시킬 때 나타나는 S. aureus의 자체응집을 방지키 위한 분석과 함께 cogglutination의 최적 조건을 확립하였다. 적정화된 coagglutination기법을 실험실과 현장에서의 edwardsiellosis의 진단에 응용하였을 때 약 $10\;{\mu}g/m1$의 E. tarda까지 검출 할 수 있었다. 더구나 이 방법은 E. tarda의 FKC, EDTA또는 열탕추출 항원에 대해서 까지 좋은 진단결과를 보여주었다. 현장에서 edwardsiellosis에 감염된 어류로부터 직접 분리된 E. tarda 균주들은 토끼 항체생성을 위해 사용된 E. tarda 219와 응집항체법및 cogglutination법에서 모두 교차반응을 보여 주었다. 이러한 교차반응의 정도는 현장에서 나타나는 여러다른 E. tarda 균주에 감염 될 수 있는 어류의 질병진단에 사용하기에 충분한 정도로 나타났으며 감염어의 조직마쇄물을 1000배 이상 희석하여도 토끼 항 E. tarde 항체로 감작시킨 S. aureus와 coagglutination 될수 있는 양의 E. tarda를 함유하고 있었다. 자연감염 또는 인위감염된 넙치, 틸라피아의 조직마쇄물, 열탕추출항원에 대한 이 방법의 적용 결과는 본방법이 특별한 장비없이 현장에서 질병진단 기법으로 사용할 수 있다는 것을 보여 준다.

• 한국식품위생안전성학회지
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• 제10권2호
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• pp.73-79
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• 1995

Incidents of seafood and massive fish kills have been rapidly increasing in both frequency and geographical distribution and the socioeconomic impacts brought by those incidents. However, the biological origins of those marine toxins have not been well clarified. Most of the marine organisms investigated are filter-feeder, which accumulate toxins from their food and/or their symbiotic microalgae. We have examined the action on rabbit platelets of marine toxins isolated from cultured dinoflagellates and sponge collected at Okinawa. Maitotoxin (MTX) is a water-soluble toxin isolated from the cultured dinoflagellate Gambierdiscus toxicus which causes a seafood poisoning in tropical regions. Zooxanthellatoxin A (ZT-A) was isolated from exteracts of cultured symbiotic dinoflagellate Symbiodinium sp. (socalled zooxanthella) from flatworms of the genus Amphiscolops collected at Okinawan marine sponge Theonella sp. MTX caused a disaggregation and a dissolution of large aggregates. ZT-A caused a dissolution of small aggregates followed by a increment of light tranmission. TZ-A caused an initial and transient shape change followed by a sustained aggregation and a increment of large aggregates. In conclusion, marine toxins exert unique patterns on the light trasmission and the size of aggregates in rabbit platelets by their concentrations and kinds

• 수산해양기술연구
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• 제57권1호
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• pp.10-24
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• 2021

A seasonal characteristic of fish assemblages in the coastal waters of Gapa-do was determined using samples collected by a trammel net from 2015 to 2019. Annual mean temperature and salinity were 18.9℃, 33.6 psu (2015), 19.5℃, 33.6 psu (2016), 18.9℃, 33.8 psu (2017), 18.8℃, 34.5 psu (2018) and 19.5℃, 33.7 psu (2019), which show a stable water mass. During the survey period, a total of 50 species (30 families and 8 orders) of fishes were identified, including Scorpaeniformes (3 families and 9 species) and Perciformes (18 families and 29 species) that accounted for 68% of the total number. The number of species was higher in summer than in winter, showing the peak in the summer by 35 species and the low in the winter by 20 species. The number of individuals and the biomass peaked in the summer by 690 individuals and 151.4 kg, and were the lowest in the autumn by 336 individuals and 76.6 kg. The annual diversity index, evenness index and richness index were appeared to be 2.54-2.75, 0.68-0.76 and 5.32-6.65 at each stations. The dominance index was appeared to be the highest in 2018 and the lowest in 2015. Marbled rockfish (Sebastiscus marmoratus), rock fish (Sebastes inermis), filefish (Stephanolepis cirrhifer), rabbit fish (Siganus fuscescens), porcupine fish (Diodon holocanthus) and smallscale blackfish (Girella melanichthys) were dominant species, which occurred abundantly during the entire survey period. Subtropical fishes were identified as a total of 24 species (48.0%) and the number of species was higher in 2019 by 20 (60.6%) species and lower in 2015 by 13 (44.8%) species. The dominant species of the subtropical fishes were appeared to be rabbit fish, porcupine fish, smallscale blackfish, flag fish (Goniistius zonatus), stripey (Microcanthus strigatus), grunter (Parapristipoma trilineatum), and bluestriped angelfish (Chaetodontoplus septentrionalis) at the study sites.