• Environmental Analysis Health and Toxicology
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• v.19 no.1
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• pp.59-64
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• 2004

Metallothionein gene expression of diesel exhaust particles (DEP) was investigated in human lung epithelial cell line. DEP was collected from diesel motor bus and soluble fraction in water was obtained. Cells, grown to near confluence, were exposed to 5-50 ppm DEP for 6 hours. Regarding the metallothionein gene expressions, MT-1 and MT-2 were induced in the DEP-treated cell by using RT-PCR and real-time PCR. However, MT-3 which is known to be brain specific, and another isoform MT-4 were not expressed in cadmium-treated groups as well as control group. Heavy metal of DEP was also analyzed and Zn was found as the major component of heavy metals in DEP used in this study.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.21 no.12
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• pp.695-702
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• 2009

Dynamic behavior of Libr-water absorption system using low-temperature hot water was investigated numerically. Thermal-hydraulic model of single-effect/double-lift 100 RT chiller was developed by applying transient conservation equations of total mass, Libr mass, energy and momentum to each component. Transient variations of system properties and transport variables were analysed during start-up operation. Numerical analysis were performed to quantify the effects of bulk concentration and part-load operation on the system performance in terms of cooling capacity, coefficient of performance, and time constant of system. For an absorption chiller considered in the present study, optimum bulk concentration was found to exist, which resulted in the minimum time constant with stable cooling capacity. COP and time constant increased as the load decreased down to 40%, below which the time constant increased abruptly and COP decreased as the load decreased further.

• Bulletin of the Korean Space Science Society
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• 2010.04a
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• pp.32.3-32.3
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• 2010

We have analyzed times of minima for six eclipsing binary systems which show asymmetric light curves. We found that five binary systems show period decrease and one system shows cyclic period variation. Three asymmetric light curves (SV Cen, RT Scl and VW Boo) are due to hot spot caused by mass transfer. Other three asymmetric light curves (AD Phe,, EZ Hya and TY Boo) are due to cool spot on the cooler component caused by magnetic activities. We also obtain absolute dimensions from photometric solution and spectroscopic solution by analyzing their light curves and radial velocity curves, collected from literatures, using 2007 version Wilson and Devinney computer code.

• Journal of Welding and Joining
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• v.5 no.3
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• pp.28-37
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• 1987

To clarify the debonding phenomena of clad steel, the effect of thermal treatment (temperature, holding time) on the interfacial strength of clad steel was preliminarily investigated. From this study, it was confirmed that the interfacial strength of clad steel was deteriorated by thermal treatment and the amount of strength deteriorated, depending on the condition of thermal treatment, could be evaluated by the following equation. ${\sigma}_{ HT}/{\sigma}_{i}/=A_{0}-A\;exp(-Q/RT)log(t/t_{0})$ This equation implies that temperature has a far strong effect on strength deterioration than tiem. The deterioration of interfacial strength of clad steel after thermal treatment may be derived from the thermal stress caused by the difference in thermal expansion coefficient between component materials and microstructural change along the interface.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.6
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• pp.1252-1257
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• 1997

In order to investigate the immunomodulatory mechanism of white ginseng, the effects of total saponin of Ginsenoside Rb$_2$component on the phagocytosis and reactive oxygen intermediate(ROI) production of mouse peritoneal macrophages were studied. Both phagocytosis assay nitrobluetetrazolium reduction test showed 20$\mu\textrm{g}$/ml concentration of total saponin significantly increased the activity of phagocytosis and production of ROI. Also cytokine gene expression of the macrophages was analyzed using reverse transcription polymerase chain reaction. In the RT-PCR assay, 20$\mu\textrm{g}$/ml concentration of either total saponin or Ginsenoside Rb$_2$increased IL-1 and TNF expression of the macrophages.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.16 no.10
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• pp.990-998
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• 2004

Dynamic behavior of an ammonia-water absorption system was investigated numerically. Thermal-hydraulic model for a single-effect 3 RT chiller was developed by applying transient conservation equations of total mass, $NH_3$ mass, energy and momentum to each component. Transient variations of system properties and transport variables were analysed during start-up operation. Numerical analyses were performed to quantify the effects of bulk concentration and charging ratio on the system performance in terms of cooling capacity, coefficient of performance, and time constant of system. For an absorption chiller considered in the present study, optimum charging ratio and bulk concentration were to found to exist, which resulted in the maximum cooling capacity and COP. The time constant increased as the charging ratio increased, but decreased with the increase of bulk concentration.

• Food Science and Industry
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• v.51 no.1
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• pp.16-25
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• 2018

Recently, the importance of analytical techniques related to food safety is emerging in the food industry due to changes in diet patterns, environmental changes, climate change and consumer's interest in food safety. In particular, food safety accidents in the food industry may cause economic losses such as media reports, product recalls, consumer distrust, and so on. Therefore, a systematic, proactive and comprehensive food safety management system is increasingly required to prevent food safety issues. Efforts to ensure the reliability of food safety are essential by introducing various analysis instruments such as LC, GC, ICP, LC/MS/MS, GC/MS/MS, ICP/MS, PCR, and RT-PCR. In addition, recent food safety analytical techniques used in food industry should be shifted paradigm by developing multi-component simultaneous analytical method, low cost with high efficient analytical method, and eco-friendly method.

• Journal of Microbiology and Biotechnology
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• v.32 no.8
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• pp.1047-1053
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• 2022

When ptsG, a glucose-specific phosphotransferase system (PTS) component, is deleted in Escherichia coli, growth can be severely poor because of the lack of efficient glucose transport. We discovered a new PTS transport system that could transport glucose through the growth-coupled experimental evolution of ptsG-deficient E. coli C strain under anaerobic conditions. Genome sequencing revealed mutations in agaR, which encodes a repressor of N-acetylgalactosamine (Aga) PTS expression in evolved progeny strains. RT-qPCR analysis showed that the expression of Aga PTS gene increased because of the loss-of-function of agaR. We confirmed the efficient Aga PTS-mediated glucose uptake by genetic complementation and anaerobic fermentation. We discussed the discovery of new glucose transporter in terms of different genetic backgrounds of E. coli strains, and the relationship between the pattern of mixed-acids fermentation and glucose transport rate.

• YAKHAK HOEJI
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• v.52 no.1
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• pp.73-78
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• 2008

Immune system can protect host attacking from a variety of microorganism and virus through innate and adaptive immunities. The innate immune system can be activated by recognition of conserved carbohydrates on the cell surface of pathogen resulting in protection, immunity regulation and inflammation. Immunostimulating and anti-tumor ${\beta}$-glucan, major cell wall component of many fungi, could be recognized as pathogen associated molecular pattern (PAMP) by C-type lectin such as pathogen recognition receptor (PRR) of host innate immunity cells. In spite of many studies of basidiomycetes ${\beta}$-glucan on immunostimulation, little is known about the precise mechanism as molecular-level. Among C-type lectins, dectin-1 was cloned and reported as a ${\beta}$-glucan receptor. In this report, we demonstrated induction of cytokine gene transcription by Ganoderma lucidum ${\beta}$-glucan in the absence or presence of lipopolysaccharide (LPS) by RT-PCR analysis. The expression of murine dectin-1 (MD-1) on RAW264.7 macrophage by RT-PCR showing both the full length, 757 bp $(MD-1{\alpha})$ and alternative spliced form, 620 bp $(MD-1{\beta})$. Both $MD-1{\alpha}$ and $MD-1{\beta}$ mRNAs were induced by ${\beta $-glucan both in the absence and presence of LPS. To explore expression of inflammatory cytokines by ${\beta}$-glucan, RAW264.7 cells were treated with ${\beta}$-glucan for 12 hours. As a result, the expressions of IL-1 IL-6, IL-l0 and $TNF-{\alpha}$ were increased by ${\beta}$-glucan treatment in a dose-dependent fashion. From these results, ${\beta}$-glucan induced transcriptions of dectin-1 and immune activating cytokine genes, indicating induction of immune allertness by expressing dectin-1 and secreting inflammatory cytokines.

• Journal of muscle and joint health
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• v.10 no.2
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• pp.188-202
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• 2003

The aim of study was to investigate the effects of 12 forms of Sun-style Tai-Chi for arthritis(TCA) on physical symptom(pain, tenderness, swelling fatigue, and blood pressure), physical function(balance, flexibility, grip strength) and social-psychological health status in osteoarthritis(OA) patients and rheumatoid arthritis(RA) patients. This study was done with one group pretest-posttest design. A total 21 arthritis patients(6 OA patient and 15 RA patient) participated in 60 minute session consisting of warm-up exercise 12-main movement including qigong, and cool-down exercise twice a week for 6weeks. Physiological and social-psychological variables were measured before and after TCA 12 form. The researcher who completed a Tai Chi workshop for exercise leaders in Australia and Seoul taught the Tai Chi movement step by step until the patient felt comfort enough to perform them correctly by themselves during the first 4 weeks. At the last 2 weeks, the subject perform 10-15set of these at a session with traditional music to help patients move in a slow tempo. TCA 12 forms program showed significant improvement in fatigue(p=0.039) of rheumatoid arthritis patient and pain(p=0.006), fatigue(p=0.013), tenderness(p=0.032), flexibility(rt arm up, p=0.014, If arm up, P=0.003), grip strength(rt hand, p=0.002, If hand, P=0.003) of osteoarthritis patients. With assessment by Arthritis Impact Measurement Scale 2 questionnaire(AIMS2), physical component(0.002) and social interaction(0.025) on osteoarthritis patients were significantly improved. Evaluation of the participants about TCA exercise noted that the exercise was fairly easy(23.6%), moderately difficult(57.1%), and fairly difficult(14.3%). Considering the result of this study, TCA exercise program was more effective in pain, tenderness, grip strength, flexibility, and physical and social interaction on osteoarthritis patients than rheumatoid arthritis patients. Tai-Chi for arthritis 12 form was an appropriate intensity exercise for osteoarthritis patients.