• Applied Biological Chemistry
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• v.45 no.4
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• pp.190-194
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• 2002

Expression of Bacillus subtilis glutamyl-tRNA synthetase (GluRS) in Escherichia coli is lethal for the host, probably because this enzyme misaminoacylates ${tRNA_l}^{Gln}$ with glutamate in vivo. In order to overexpress B. subtilis GluRS, encoded by the gltX gene, in E. coli, this gene was amplified from B. subtilis 168 chromosomal DNA using PCR method and the entire coding region was cloned into a pET11a expression vector so that it was expressed under the control or the T7 Promoter. The resulting recombinant pEBER plasmid was transformed into E. coli Novablue (DE3) bearing the T7 RNA polymerase gene for expression. After IPTG treatment, the overproduced enzyme was purified using ammonium sulfate fractionation, Source Q anion exchange chromatography, Superdex-200 gel filtration, and Mono Q anion exchange chromatography. The purified enzyme yielded 18-fold increase in specific activity over the crude cell extract and its molecular weight was approximately 55 kDa on SDS-PAGE.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.19
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• pp.8319-8324
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• 2014

Although genetic markers identifying women at an increased risk of developing breast cancer exist, the majority of inherited risk factors remain elusive. Mutations in the BRCA1/BRCA2 gene confer a substantial increase in breast cancer risk, yet routine clinical genetic screening is limited to the coding regions and intronexon boundaries, precluding the identification of mutations in noncoding and untranslated regions. Because 3' untranslated region (3'UTR) polymorphisms disrupting microRNA (miRNA) binding can be functional and can act as genetic markers of cancer risk, we aimed to determine genetic variation in the 3'UTR of BRCA1/BRCA2 in familial and early-onset breast cancer patients with and without mutations in the coding regions of BRCA1/BRCA2 and to identify specific 3'UTR variants that may be risk factors for cancer development. The 3'UTRs of the BRCA1 and BRCA2 genes were screened by heteroduplex analysis and DNA sequencing in 100 patients from 46 BRCA1/2 families, 54 non-BRCA1/2 families, and 47 geographically matched controls. Two polymorphisms were identified. SNPs $c.^*1287C$ >T (rs12516) (BRCA1) and $c.^*105A$ >C (rs15869) (BRCA2) were identified in 27% and 24% of patients, respectively. These 2 variants were also identified in controls with no family history of cancer (23.4% and 23.4%, respectively). In comparison to variations in the 3'UTR region of the BRCA1/2 genes and the BRCA1/2 mutational status in patients, there was a statistically significant relationship between the BRCA1 gene polymorphism $c.^*1287C$ >T (rs12516) and BRCA1 mutations (p=0.035) by Fisher's Exact Test. SNP $c.^*1287C$ >T (rs12516) of the BRCA1 gene may have potential use as a genetic marker of an increased risk of developing breast cancer and likely represents a non-coding sequence variation in BRCA1 that impacts BRCA1 function and leads to increased early-onset and/or familial breast cancer risk in the Turkish population.

• Molecules and Cells
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• v.43 no.8
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• pp.718-727
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• 2020

The imbalance between the proliferation and apoptosis of B-cell precursors is an important contributor to the pathogenesis of B-cell precursor acute lymphoblastic leukemia (BCP-ALL), while its specific regulatory mechanism remains perplexing. This study aimed to expound the underlying mechanism of the proliferation and apoptosis of BCP-ALL cells from the perspective of non-coding RNA. In this study, long non-coding RNA colorectal neoplasia differentially expressed (LncRNA CRNDE) was upregulated in the bone marrow of BCP-ALL patients and BCP-ALL cell lines (NALM-6 and RS4;11). Functionally, LncRNA CRNDE knockdown restrained cell proliferation and boosted cell apoptosis in NALM-6 and RS4;11 cells. The subsequent investigation confirmed that LncRNA CRNDE bound to miR-345-5p and negatively regulated miR-345-5p expression. The overexpression of miR-345-5p suppressed cell proliferation and boosted cell apoptosis in NALM-6 and RS4;11 cells. Further experiments revealed that miR-345-5p downregulated cyclic AMP response element-binding protein (CREB) expression by targeting its mRNA directly. CREB overexpression reversed the effect of miR-345-5p mimic on cell proliferation and apoptosis in NALM-6 and RS4;11 cells. Finally, in vivo experiments showed that LncRNA CRNDE knockdown prolonged the survival of mice xenotransplanted with NALM-6 cells. In conclusion, LncRNA CRNDE upregulated CREB expression by suppressing miR-345-5p, thus promoting cell proliferation and reducing cell apoptosis in BCP-ALL.

• Korean Journal of Microbiology
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• v.55 no.2
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• pp.174-176
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• 2019

A novel bacterium, designated strain RR4-38 (= KCTC 52651 = DSM 108068), belonging to the family Flavobacteriaceae was isolated from a biofilter in the seawater recirculating aquaculture system in South Korea. A single complete genome contig which is 3,182,272 bp with 41.9% G+C content was generated using PacBio RS II platform. The genome includes 2,829 protein-coding genes, 6 rRNA genes, 38 tRNA genes, 4 non-coding RNA genes, and 9 pseudogenes. The results will provide insights for understanding microbial activity in the seawater recirculating aquaculture system.

• Journal of the Korea Institute of Military Science and Technology
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• v.9 no.4
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• pp.39-49
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• 2006

LYNX ESM has not only ESM function but Datalink function. The LYNX boarded equipment Interface and Channel coding is needed for LYNX Datalink to support the mother ship with tactical data included threat radar analysis data of LYNX ESM, navigation data, Radar and Sonar of LYNX. The interface of loaded equipments are various protocol like as ARINC419/429, RS232, Synchro, Sync Serial. The Channel coding of Convolution-Scrambler-Interleaver-Bit Stuffing is applied for recovering the transmission data mixed with propagation errors in sea environment.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.11 no.6
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• pp.70-80
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• 2012

In this paper, we consider the amplify-and-forward relaying systems with the relay selection (AF-RS) using full channel information (FCI) and using partial channel information (PCI), respectively, in which the direct communication link between a source and a destination is available. And, the outage probabilities for the AF-RS using FCI and using PCI are investigated in Rayleigh fading channels, respectively. We provide exact outage probabilities, tight outage probability lower bounds, and asymptotic outage probabilities, respectively. And also, we present coding gains achievable by the AF-RS using FCI and using PCI. Through numerical investigation, all the analytic expressions are verified, and the coding gains are respectively evaluated with different average channel powers for the first and the second hop as well as different numbers of relays.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.8 no.4
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• pp.342-353
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• 1997

This paper presents an analytical evaluation of a hybrid direct-sequence/slow frequencyhopped code division multiple-access (DS/SFH-CDMA) system employing noncoherent M-ary frequency shift keying(MFSK) modulation in a multiple Nakagami fading (m) environment. Multipath interference (MPI) and multi-access interference (MAI) is taken into account and the spectral efficiency is calculated for uncoded as well as channel coding systems. Predetection multipath maximal ratio combining (MRC) diversity in conjunction with interleaved channel coding (Hamming(7,4) code, BCH(15, 7) code and RS (7, 4), (15, 9)) code ) is employed for improving the bit error rate (BER) performance. The BER of noncoherent hybrid system is obtained using a Gaussian interference approximation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.6
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• pp.1115-1125
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• 1994

In this paper, an efficient concatenated coding scheme under the satellite channel is presented. The performance of this scheme in terms of bit error rate versus energy per information bit over white gaussian noise power density E/N has been evaluated via computer simulation as a function of various system parameters. To achieve accuracy in simulation results, the distortions caused from the satellite channel, such as the nonlinearity of the TWTA(traveling wave tube amplifier), signal distortions of the input and output filters, has been considered. The simulation results show that, through using the 2/3 punctured convolutional code as the inner code of the concatenated code system, the coding rate can be improved more over 16%, while maintaining the same system complexity and bit error performance.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.8
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• pp.1234-1240
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• 2002

In this paper, we analyze the block error performance of the CDMA system, whose information data are spread spectrum with PN sequences, and the chip signals are modulated with BPSK signal, combining MRC(Maximum Ratio Combine) diversity techniques with repetition transmission, FEC(Forward Error Correction) code in mobile communication channel which is characterized by Nakagami fading. As a results of study, the coding techniques provide more efficient improvement than a diversity techniques, but coding techniques are required the adding bandwidth as many coding rate. Also, when the system is combined MRC diversity techniques with coding techniques, the amount of improvement is dramatically increased.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.11
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• pp.1229-1234
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• 2010

High quality and high speed multimedia services will be required in the next generation mobile communications. To satisfy these services, powerful error correction techniques are used. ARQ(Auto Repeat Request) techniques, aspects of the throughput, and FEC(Forward Error Correct) techniques have disadvantages in terms of reliability. H-ARQ(Hybrid Auto Repeat Request) systems have been studied extensively as a solution of these disadvantages. In this paper introduce double layer coding and H-ARQ type, proposed H-ARQ system using double layer coding methods. Simulate BER performance and throughput. And we suggest that according to channel condition suitable H-ARQ type.