• Nutrition Research and Practice
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• v.12 no.6
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• pp.494-502
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• 2018

BACKGROUND/OBJECTIVES: Reducing the number of adipocytes by inducing apoptosis of mature adipocytes as well as suppressing differentiation of preadipocytes plays an important role in preventing obesity. This study examines the anti-adipogenic and pro-apoptotic effect of red pepper seed water extract (RPS) prepared at $4^{\circ}C$ (RPS4) in 3T3-L1 cells. MATERIALS/METHODS: Effect of RPS4 or its fractions on lipid accumulation was determined in 3T3-L1 cells using oil red O (ORO) staining. The expressions of AMP-activated protein kinase (AMPK) and adipogenic associated proteins [peroxisome proliferator-activated receptor-${\gamma}$ ($PPAR-{\gamma}$), CCAAT/enhancer-binding proteins ${\alpha}$ (C/EBP ${\alpha}$), sterol regulatory element binding protein-1c (SREBP-1c), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC)] were measured in 3T3-L1 cells treated with RPS4. Apoptosis and the expression of Akt and Bcl-2 family proteins [B-cell lymphoma 2 (Bcl-2), Bcl-2-associated death promoter (Bad), Bcl-2 like protein 4 (Bax), Bal-2 homologous antagonist/killer (Bak)] were measured in mature 3T3-L1 cells treated with RPS4. RESULTS: Treatment of RPS4 ($0-75{\mu}g/mL$) or its fractions ($0-50{\mu}g/mL$) for 24 h did not have an apparent cytotoxicity on pre and mature 3T3-L1 cells. RPS4 significantly suppressed differentiation and cellular lipid accumulation by increasing the phosphorylation of AMPK and reducing the expression of $PPAR-{\gamma}$, C/EBP ${\alpha}$, SREBP-1c, FAS, and ACC. In addition, all fractions except ethyl acetate fraction significantly suppressed cellular lipid accumulation. RPS4 induced the apoptosis of mature adipocytes by hypophosphorylating Akt, increasing the expression of the pro-apoptotic proteins, Bak, Bax, and Bad, and reducing the expression of the anti-apoptotic proteins, Bcl-2 and p-Bad. CONCLUSIONS: These finding suggest that RPS4 can reduce the numbers as well as the size of adipocytes and might useful for preventing and treating obesity.

• Korean Journal of Plant Resources
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• v.30 no.5
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• pp.522-534
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• 2017

This study was investigated genetic variation in 24 Taraxacum accessions from various regions in South Korea based on the sequences of two chloroplast DNA (cpDNA) regions (trnL-trnF and rps16-trnK). T. mongolicum, T. officinale, and T. laevigatum were triploid, and T. coreanum and T. coreanum var. flavescens were tetraploid. The trnL-trnF region in native Korean dandelions (T. mongolicum, T. coreanum, and T. coreanum var. flavescens) were ranged from 931 to 935 bp in length, and that of naturalized dandelions were ranged from 910 bp (T. officinale) to 975 bp (T. laevigatum) in length. The rps16-trnK region in T. mongolicum, T. coreanum, T. coreanum var. flavescens, T. officinale, and T. laevigatum was 882-883 bp, 875-881 bp, 878-883 bp, 874-876 bp, and 847-876 bp, respectively, in length. The sequence similarity matrix of the trnL-trnF region ranged from 0.860 to 1.00 with an average of 0.949, and that of the rps16-trnK region ranged from 0.919 to 1.000 with an average of 0.967. According to the phylogenetic analysis, the Korean native taxa and naturalized taxa were divided independent clade in two cpDNA region. T. coreanum var. flavescens clustered only with T. coreanum, and there were no significant differences in their nucleotide sequences. The finding that two accessions (T. coreanum; Jogesan, T. mongolicum; Gangyang) had a high level of genetic variation suggests their utility for breeding materials.

• 한국신재생에너지학회:학술대회논문집
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• 2008.10a
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• pp.372-374
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• 2008

Korea classified into a development country when UNFCCC was concluded in 1995. So Korea doesn't have a GHG reduction duty until 2012. As the UNFCCC is strengthened, recently there is a growing interest in renewable energy and energy usage efficiency improvement for reducing GHG emission. It is associated with CES and renewable energy. CES is a total energy (heat, cooling and power)supplier in aggregated demand zone like a hotel, building, hospital and redevelopment district using CHP and it improves energy usage efficiency. At present, renewable energy is needed for GHG reduction duty but renewable energy doesn't have economic feasibility. So renewable energy is needed various support system to popularize which is a FIT and RPS. Especially RPS is carrying out instead of FIT in many advanced country and it will be inroduced in Korea. RPS is a duty which electricity service provider must guarantee renewable energy as much as specific ratio of total capacity. Therefore this study conducts an economic feasibility estimation of CES considering renewable energy when RPS will introduced in the future.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.53 no.5
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• pp.275-284
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• 2004

This paper analyzes the environmental impact and economic effect of introducing the Renewable Portfolio Standard (RPS) into Korean electricity market using the DECADES (Database and Methodologies for Comparative Assessment of Different Energy Source for Electricity Generation) model, a comparative assessment tool developed by IAEA. A bottom up approach is adopted for the evaluation of air pollutant emission and its impact of several RPS scenarios. The environmental damage costs of RPS scenarios are evaluated based on the Extern-E results and Thailand externality study carried by EGA T. The results of this study can be applied in determining or analyzing the national electricity policy and energy policy.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2001.05a
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• pp.30-30
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• 2001

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.9
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• pp.752-759
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• 2018

This paper presents methods and results of nonlinear simulations for a guided munition for verifying stability at slow spin. The munition is launched by an artillery and it deploys the rear fins to reduce its spin. While the spin speed command is set to 1 rps and 3 rps, wind gusts of 3m/s, 7m/s, 10m/s, and 15m/s in amplitude, and 26 different directions were generated as disturbance for each simulation run. Whereas the munition with the spin speed of 3 rps didn't flip, that with 1-rps spin flipped under some gusts. However, the gusts which increase airspeed in the flight direction didn't introduce harmful effect. Most importantly, all the flips of the munition was observed near the end of the simulation where the munition is going down. No problem was observed near the summit of trajectory.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2001.05a
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• pp.32-32
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• 2001

• Tuberculosis and Respiratory Diseases
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• v.80 no.2
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• pp.159-168
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• 2017

Background: Streptomycin (SM) is recommended by the World Health Organization (WHO) as a part of standard regimens for retreating multidrug-resistant tuberculosis (MDR-TB) cases. The incidence of MDR-TB in retreatment cases was 19% in Thailand. To date, information on SM resistance (SMR) gene mutations correlated to the SMR of Mycobacterium tuberculosis Thai isolates is limited. In this study, the mutations in rpsL, rrs, gidB, and whiB7 were investigated and their association to SMR and the lineage of M. tuberculosis were explored. Methods: The lineages of 287 M. tuberculosis collected from 2007 to 2011 were identified by spoligotyping. Drug susceptibility profiles were evaluated by the absolute concentration method. Mutations in SMR genes of 46 SM-resistant and 55 SM-susceptible isolates were examined by DNA sequencing. Results: Three rpsL (Lys43Arg, Lys88Arg, and Lys88Thr) and two gidB (Trp45Ter and Gly69Asp) mutations were present exclusively in the SM resistant M. tuberculosis. Lys43Arg rpsL was the most predominant SMR mutations (69.6%) and prevailed among Beijing isolates (p<0.001). No SMR-related mutation in was found rrs. The combination of rpsL and gidB mutations provided 76.1% sensitivity for detecting SMR in M. tuberculosis Thai isolates. whiB7 was not responsible for SMR in SM resistant isolates lacking rpsL and rrs mutations. The significance of the three gidB mutations, 276A>C, 615A>G, and 330G>T, as lineage signatures for Beijing and EAI were underscored. This study identified 423G>A gidB as a novel sub-lineage marker for EAI6-BGD1. Conclusion: Our study suggested that the majority of SMR in M. tuberculosis Thai isolates were responsible by rpsL and gidB polymorphisms constantly providing the novel lineage specific makers.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.11a
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• pp.191-196
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• 1996

The potential value of the Application Specific Integrated Circuits(ASIC's) in safety systems of Nuclear Power Plants(NPP's) is being increasingly recognized because they are essentially hardwired circuitry on a chip, the reliability of the system can be proved more easily than that of software based systems which is difficult in point of software V&V(Verification and Validation). There are two types of ASIC, one is a full customized type, the other is a half customized type. PLD(Programmable Logic Device) used in this paper is a half customized ASIC which is a device consisting of blocks of logic connected with programmable interconnections that are customized in the package by end users. This paper describes the RPS(Reactor Protection System) composed of ASICs which provides emergency shutdown of the reactor to protect the core and the pressure boundary of RCS(Reactor Coolant System) in NPP's. The RPS is largely composed of five logic blocks, each of them was implemented in one PLD, as the followings. A). Bistable Logic B). Matrix Logic C).Initiation Logic D). MMI(Man Machine Interface) Logic E). Test Logic.

• Fisheries and Aquatic Sciences
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• v.26 no.10
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• pp.605-616
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• 2023

Scuticociliatosis, caused by the parasitic pathogen Miamiensis avidus, poses a significant threat to olive flounder farms in South Korea. Infected fish suffer from severe systemic infections affecting various organs, with potential secondary bacterial diseases. This study investigated the emergence of different M. avidus serotypes in 20 olive flounder farms on Jeju island, South Korea, from 2015 to 2020. Additionally, we identified Tenacibaculum maritimum as a co-infecting bacteria. Based on serotyping and monitoring data, we developed a trivalent vaccine targeting two serotypes of M. avidus and one strain of T. maritimum. The efficacy of the vaccine was evaluated under laboratory conditions and demonstrated a relative percentage of survival (RPS) of 75%, 80%, and 93% for M. avidus serotype I, M. avidus serotype II and T. maritimum, respectively. Furthermore, successful field trials conducted on four different olive flounder farms resulted in significantly higher survival rates (52%-76% RPS) and weight gains in vaccinated fish. Overall, this study presents an effective vaccine against M. avidus and T. maritimum infections in farmed olive flounder, making a valuable contribution to sustainable aquaculture in South Korea.