• The Plant Pathology Journal
• /
• 제25권4호
• /
• pp.422-428
• /
• 2009

Studies were conducted to identify the sources of resistance in mungbean recombinant inbred lines (RILs) in Thailand against mungbean yellow mosaic disease (MYMD). 146 mungbean RILs in $F_8$ series were evaluated in a field including resistant parent NM-10-12-1 and susceptible parent KPS 2 during summer 2008 under high inoculum pressure. The RILs were subsequently scored for disease symptom severity ratings (DSSR) using a new scale. Observations regarding DSSR and % disease index (%DI) showed that the tested RILs responded differently to the disease. A large number of RILs (132) were found highly susceptible, 12 were susceptible, 3 were tolerant and one was resistant. Overall screening results showed that three RILs, viz. line no. 30, 100 and 101 had minimum DSSR and % disease index thus they are good source of resistance to MYMD in spite of high disease pressure and can therefore be used directly as varieties to manage the disease in Thailand.

• The Plant Pathology Journal
• /
• 제31권4호
• /
• pp.402-413
• /
• 2015

Yellow rust (stripe rust), caused by Puccinia striiformis f. sp. tritici, is one of the most destructive foliar diseases of wheat in Egypt and worldwide. In order to identify wheat genotypes resistant to yellow rust and develop molecular markers associated with the resistance, fifty F8 recombinant inbred lines (RILs) derived from a cross between resistant and susceptible bread wheat landraces were obtained. Artificial infection of Puccinia striiformis was performed under greenhouse conditions during two growing seasons and relative resistance index (RRI) was calculated. Two Egyptian bread wheat cultivars i.e. Giza-168 (resistant) and Sakha-69 (susceptible) were also evaluated. RRI values of two-year trial showed that 10 RILs responded with RRI value >6 <9 with an average of 7.29, which exceeded the Egyptian bread wheat cultivar Giza-168 (5.58). Thirty three RILs were included among the acceptable range having RRI value >2 <6. However, only 7 RILs showed RRI value <2. Five RILs expressed hypersensitive type of resistance (R) against the pathogen and showed the lowest Average Coefficient of Infection (ACI). Bulked segregant analysis (BSA) with eight simple sequence repeat (SSR), eight sequence-related amplified polymorphism (SRAP) and sixteen random amplified polymorphic DNA (RAPD) markers revealed that three SSR, three SRAP and six RAPD markers were found to be associated with the resistance to yellow rust. However, further molecular analyses would be performed to confirm markers associated with the resistance and suitable for marker-assisted selection. Resistant RILs identified in the study could be efficiently used to improve the resistance to yellow rust in wheat.

• 생물환경조절학회지
• /
• 제15권4호
• /
• pp.421-427
• /
• 2006

밀양 23호/기호벼 유래 164 RILs($F_{17}$)을 이용하여, 종자의 휴면과 관련이 있는 수발아 저항성, 건열 저항성 및 종자수명을 검정하고, 이들 상호간 상관관계를 조사하였다. 출수 40일째에 밀양23호와 기호벼의 수발아율은 0%, 7%이었고, M/G RILs은 0$\sim$50.9%의 범위를 보였으며, 평균 3.2%였다. $90^{\circ}C$, 24시간 건열처리후 밀양23호와 기호벼는 99.2%, 37.6%의 발아율을 보였고, $T_{50}$은 2.7일, 12.9일로 유의한 차이를 보였으며, M/G RILs의 평균 발아율은 72.4%였다. 상온저장 한 종자수명은 밀양23호는 수확후 3.5년까지도 80% 이상의 종자 발아율을 유지하였으나, 기호벼는 18개월 후부터 급격히 발아력이 감소하여(30%이하), 42개월 후 발아력을 완전히 상실하였다. M/G RILs의 종자활력은 수확후 42개월까지 71계통이 90%이상의 발아율을 보였다. 수발아 저항성, 건열 저항성 및 종자수명은 밀양 23호와 기호벼 간에 뚜렷한 품종간 차이를 보였으며, M/G RILs은 양친의 중간값에 가까운 평균값을 보였다. 건열저항성과 종자수명 간에는 고도로 유의한 정의 상관관계를 보였고, 수발아와 종자수명, 수발아와 건열저항성 간에는 부의 상관관계가 성립하였다.

• Plant Breeding and Biotechnology
• /
• 제5권4호
• /
• pp.371-389
• /
• 2017

Supplying sufficient rice to growing populations is a global challenge. Hybrid indica rice varieties exploiting heterosis have increased yields, but inter-subspecific crosses between indica and japonica varieties are hampered by sterility. Examination and genetic understanding of yield heterosis in indica/japonica crosses addressing yield barriers are basic requirements. In this study, QTLs for heterosis of yield traits were identified in indica-japonica recombinant inbred lines (RILs) using a total of 178 RILs originating from Dasanbyeo (indica) ${\times}$ TR22183 (japonica) (DT-RILs) and their backcrossed populations. Nine of sixty-six major quantitative trait loci (QTLs) identified in DT-RILs exhibited heterosis. Heterosis QTLs clustered with other traits on chromosomes 1, 4, and 8, and clusters were conserved between different RILs. The clusters contained several known yield enhancement genes/QTLs. Specific heterotic allele combinations contributed to four major heterosis QTLs, particularly for panicle and spikelet number traits. Heterosis for yield and yield-related traits was explained by the harmonized effects of overdominance, dominance, and epistatic interactions in inter-subspecific breeding populations.

• 한국작물학회지
• /
• 제44권1호
• /
• pp.86-94
• /
• 1999

A set of rice recombinant inbred lines was developed from a cross between a Tongil type variety, Nonganbyeo, and an indica variety, BG276, by the single seed descent method. The number of the lines in the population was 272. All the agronomic characters studied except ADV (alkali-digestion value) showed continuous variation among the RILs, implying that their inheritance mode should be quantitative. The patterns of the variation in the RILs were either normal or skewed distribution. ADVs of RILs were segregated into two groups with 1:1 ratio, indicating that ADVs in this KIL population might be controlled by one major gene. Transgressive variations were also observed in all characters. Heritability values of the characters varied from 0.488 in brown/rough rice ratio to 0.895 in alkali-digestion value. In the analysis of genotypic and phenotypic correlations, the character of yield was positively correlated with 8 different agronomic characters. The number of panicles per hill was negatively correlated with culm length, panicle length, and number of spikelets per panicle. Grain length was positively correlated with grain width, grain thickness, grain length/width ratio, white belly, ADV, and amylose. However, grain length/width ratio was negatively correlated with grain width. White core was also negatively correlated with white belly and ADV.

• Journal of Crop Science and Biotechnology
• /
• 제10권4호
• /
• pp.249-256
• /
• 2007

A study was conducted to observe the variation and inheritance of agronomic traits and their interrelationship in mungbean. The objective of the study was to compare agronomic traits and hardseed percentage of 268 recombinant inbred lines(RILs) developed from the cross between wild Vigna subspecies sublobata "ACC 41" with the mungbean cultivar "Berken". The RIL population and their parents were evaluated under controlled conditions in a glass house at the University of Queensland, Brisbane, Australia. The results showed significant differences among the RILs and among the parents in all traits under study. Berken had a longer flowering date and a higher seed weight per plant, but less total leaf number and pod number per plant than ACC 41. A germination test between papers revealed that ACC 41 was 100% hard-seeded and did not germinate at all, while Berken germinated up to 100%. Their RILs distributed well between 0 to 100% hardseed. Upon scarification, all hardseed germinated within seven days. Narrowsense heritability estimates of total leave number, hardseedness, pod length, and pod width were highly heritable at 89.9, 98.9, 93.7, and 93.2%, respectively. The heritability of seed weight per plant and number of seeds per plant were lower at 63.1 and 58.4%, respectively. Seed weight per plant showed positive transgressive segregation when compared with ACC 41 and a positive correlation with 100 seed weight. While the number of seeds per pod showed a negative transgressive segregation when compared with Berken and a negative correlation with pod length and pod width. The RILs gave a 1:1 segregation ratio in leaflet shape, growth habit, and growth pattern, indicating that these traits were controlled by a single dominant gene.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.102-102
• /
• 2017

In this study, QTL analysis of allelopathy was conducted. A total of 171 of F8 RILs developed from the cross between Nongan(low allelopathic cultivar) and Sathi(high allelopathic cultivar) were used . the performance of allelopathy were evaluated using 'ECAM(Equal Compartment Agar Method)', where the root length of lettuce cultivated with the RILs were measured. The distribution of the performance was followed as normal distribution. In order to identify the location of QTLs related to allelopathy, QTL-seq with BSA(Bulked-segregant analysis) was performed with 20 highest and 10 lowest RILs. As a result, Two Sliding window coordinate region of candidate QTLs were detected on Chr4 (5,050,001 - 14,800,000, 18,650,001 - 22,500,000), Chr8 (2,550,001 - 8,250,000, 21,150,001 - 26,800,000) and One region on Chr7 (1 - 3,300,000), Chr9 (1 - 13,300,000) respectively.

• Plant Breeding and Biotechnology
• /
• 제6권4호
• /
• pp.444-453
• /
• 2018

The rice bran oil contained in brown rice is composed of highly valued ingredient. Improving the content of unsaturated fatty acids in rice seed, such as oleic acid, linoleic acid, and ${\alpha}$-linolenic acid, would provide more benefit to human health. Fatty acid content is quantitative trait controlled by multiple genes. We have utilized high-density SNP data from highly advanced breeding populations to identify QTLs for fatty acid contents in brown rice. Here, we identified 51 major QTLs (M-QTLs) and 25 epistatic QTLs (EpQTLs) related to eleven fatty acid contents. Eight and four M-QTLs were pleiotropically associated with the content of different fatty acids in MT-RILs and DT-RILs, respectively. Total effect of M-QTLs for palmitic acid (16:0), oleic acid (18:1), and linoleic acid (18:2), could explain phenotypic variations of 36.7%, 63.7%, and 41% in MT-RILs, respectively. Alpha-linolenic acid which is important for a human's health could be explained phenotypic variation of 15.7% by six M-QTLs. These QTLs identified in this study can be used to improve nutritious content in rice breeding programs.

• 한국작물학회지
• /
• 제50권3호
• /
• pp.205-211
• /
• 2005

본 연구는 저온에 민감한 밀양 23호를 모본으로 내냉성이 강한 Stejaree45호를 부본으로 교잡하여 육성한 175 RILs를 이용하여 내냉성 관련 형질들의 변이분포, 유전 통계량 및 상관 관계를 분석하였다. 조사된 형질 중 수당영화수, 적고, 출수지연일수, 간장단축율 및 영화수감소율은 정규분포에 가까운 연속변이 분포곡선을 보였으며, 모든 형질에서 양친의 범위를 벗어나는 초월분리현상을 보였다. 임실감소율과 수량감소율은 모본인 밀양 23호 쪽으로 일정하게 치우친 경향으로 저온에 민감한 쪽으로, 출수지연일수와 간장단축율은 부본인 Stejaree45 쪽으로 치우친 경향으로 내냉성이 증가되는 쪽으로 작용가가 큰 유전자가 관여하는 것으로 추정되었다. 내냉성 관련 형질들의 상관관계 중 수량감소율과 상관이 인정되었던 형질은 출수지연일수, 간장단축율, 이삭추출도 및 임실감소율이었고, 조사된 형질중 유전력이 $60\%$이상으로 나타난 형질은 분얼기 적고, 임실감소율, 이삭추출도 였으며, 유전력이 $40\%$ 이하로 낮았던 형질은 간장단축율, 영화수감소율 및 수량감소율이었다. 175 RILs 중에서 내냉성 관련 형질의 내냉성 정도가 부본인 Stejaree45수준이거나 그 이상의 내냉성 형질을 가진 것은 86계통으로, 1개의 내냉성 형질은 56계통 $32.0\%$였고, 2개의 내냉성 형질은 25계통 $14.3\%$, 3개의 내냉성 형질은 4계통 $2.3\%$, 4개의 내냉성 형질은 1계통 밖에 없었으며, 그 이상의 내냉성 관련 형질을 가진 계통은 없었다.

• 제어로봇시스템학회논문지
• /
• 제19권3호
• /
• pp.248-255
• /
• 2013

This paper proposes the architecture of the RILS (Robot-in-the-Loop-Simulation) consisting of the robot, the virtual robot, and the avatar robot which is the type of virtual robots operating according to the robot status and behavior. And the synchronization algorithm for mobilization part of the avatar robot is suggested, which reduces the difference between behaviors of the robot and those of the avatar robot. This difference occurs due to the environmental and mechanical mismatches between the robot and avatar robot. In order to reduce this difference in robots behaviors, the synchronization algorithm controls the avatar robot based on the data observed from the robot's behavior. The proposed architecture and the synchronization algorithm are validated from some simulation results.