• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1651-1658
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• 2020

Since Zika virus (ZIKV) was first detected in Uganda in 1947, serious outbreaks have occurred globally in Yap Island, French Polynesia and Brazil. Even though the number of infections and spread of ZIKV have risen sharply, the pathogenesis and replication mechanisms of ZIKV have not been well studied. ZIKV, a recently highlighted Flavivirus, is a mosquito-borne emerging virus causing microcephaly and the Guillain-Barre syndrome in fetuses and adults, respectively. ZIKV polyprotein consists of three structural proteins named C, prM and E and seven nonstructural proteins named NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5 in an 11-kb single-stranded positive sense RNA genome. The function of individual ZIKV genes on the host innate immune response has barely been studied. In this study, we investigated the modulations of the NF-κB promoter activity induced by the MDA5/RIG-I signaling pathway. According to our results, two nonstructural proteins, NS2A and NS4A, dramatically suppressed the NF-κB promoter activity by inhibiting signaling factors involved in the MDA5/RIG-I signaling pathway. Interestingly, NS2A suppressed all components of MDA5/RIG-I signaling pathway, but NS4A inhibited most signaling molecules, except IKKε and IRF3-5D. In addition, both NS2A and NS4A downregulated MDA5-induced NF-κB promoter activity in a dosedependent manner. Taken together, our results suggest that NS2A and NS4A signifcantly antagonize MDA5/RIG-I-mediated NF-κB production, and these proteins seem to be controlled by different mechanisms. This study could help understand the mechanisms of how ZIKV controls innate immune responses and may also assist in the development of ZIKV-specific therapeutics.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2010년도 춘계학술대회 논문집
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• pp.1345-1346
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• 2010

• Tribology and Lubricants
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• 제34권1호
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• pp.33-41
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• 2018

This paper describes a new test rig for identification of rotordynamic force coefficients of squeeze film dampers (SFDs) in automotive turbochargers (TCs). Prior studies have mainly concentrated on relatively large-sized SFDs used in aircraft engines, turbocompressors, and turbopumps. The main objective of the current study is to propose a test rig for identification of dynamic force coefficients of small-sized SFDs (a journal diameter of ~11 mm). The current test rig consists of a journal, a SFD cartridge, four support rods, an upper structure, a data acquisition (DAQ) system, and an oil circulation unit. The annular gaps between the journal outer surface and SFD cartridge inner surface create SFD film lands. The damper has two parallel film lands separated by a central groove, having an axial length and depth of 3 mm. Each film land has a length of 4 mm with a $40{\mu}m$ radial clearance. The static load and dynamic impact tests identify the structural characteristics (i.e., stiffness and natural frequency) of the journal and assembled test rig. The measurements show good agreement with predictions. The SFD performance data from this test rig will be used to develop innovative TC rotor systems with improved NVH and reliability characteristics incorporating advanced SFD technology.

• 대한기계학회논문집B
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• 제30권11호
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• pp.1057-1065
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• 2006

In order to figure out the physical meaning of 3-D angular flow index for in-cylinder bulk motion, CFD analysis for the swirl and tumble steady flow test rig were made using commercial package STAR-CD. Computer simulations and rig tests on some kinds of induced flow conditions were carried out. Finally, based on the comparison between the simulated results and measured results, the physical meaning of 3-D angular flow index $|\longrightarrow_{N_B}|$, $\beta$ composed of swirl and tumble coefficients measured by steady flow test rig was described.

• 한국철도학회논문집
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• 제16권1호
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• pp.7-13
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• 2013

철도차량의 실차 규모 동특성시험은 설비 구축, 대차의 제작 및 시험 조건의 설정등과 관련하여 비용, 시간 등의 증대로 많은 어려움이 따른다. 본 연구에서는 실험실 환경에서 차량 진동문제와 안전성을 평가하기 위한 목적으로 축소모델을 제작하고, 개발된 축소모델이 해당 철도차량의 동특성을 정밀하게 모사하는지를 검증하는 평가방법에 대한 연구를 수행하였다. 축소이론은 Jaschinski 상사기법의 이론을 적용하였으며, 1/10 축소모델을 구축하였다. 본 연구에서는 축소 대차의 상하 진동에 초점을 맞혀 시스템을 제작하였다. 시스템은 구동부, 보기, 대차 등 3개의 하부구조로 구성되었으며, 실제 차량으로 환산 시 400km/hr까지 실험가능 하도록 목표로 하였다. 축소 대차의 설계 및 제작과 함께, 동역학해석 프로그램인 ADAMS/View를 이용하여 고유치 해석을 수행하였다. 전산해석으로 도출된 고유진동수는 실험결과와 비교 분석되었다. 시스템이 가지고 있는 초기 5개의 자유도에 상응한 고유진동수를 비교한 결과, 개발된 상사 이론에 따른 축소모형은 충분한 신뢰성을 확보한 것으로 검증되었다. 본 연구에서 개발된 축소모델은 대학실험실에서 차량진동모드를 모사하는 용도로 기획되었으나, 추후 자유도의 추가보완을 통해 고속철도 차량의 대차와 보기구조 동특성 해석에 활용될 수 있을 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.226-232
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• 2021

Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging phlebovirus of the Phenuiviridae family that has been circulating in the following Asian countries: Vietnam, Myanmar, Taiwan, China, Japan, and South Korea. Despite the increasing infection rates and relatively high mortality rate, there is limited information available regarding SFTSV pathogenesis. In addition, there are currently no vaccines or effective antiviral treatments available. Previous reports have shown that SFTSV suppresses the host immune response and its nonstructural proteins (NSs) function as an antagonist of type I interferon (IFN), whose induction is an essential part of the host defense system against viral infections. Given that SFTSV NSs suppress the innate immune response by inhibiting type I IFN, we investigated the mechanism utilized by SFTSV NSs to evade IFNmediated response. Our co-immunoprecipitation data suggest the interactions between NSs and retinoic acid inducible gene-I (RIG-I) or TANK binding kinase 1 (TBK1). Furthermore, confocal analysis indicates the ability of NSs to sequester RIG-I and related downstream molecules in the cytoplasmic structures called inclusion bodies (IBs). NSs are also capable of inhibiting TBK1-interferon regulatory factor 3 (IRF3) interaction, and therefore prevent the phosphorylation and nuclear translocation of IRF3 for the induction of type I IFN. The ability of SFTSV NSs to interact with and sequester TBK1 and IRF3 in IBs demonstrate an effective yet unique method utilized by SFTSV to evade and suppress host immunity.

• 한국초전도ㆍ저온공학회논문지
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• 제11권4호
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• pp.12-15
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• 2009

The $I_c$ degradation behavior of critical current in differently processed YBCO and SmBCO CC tapes with IBAD template has been investigated. It has been known that the residual strain in the CC tape will influence the shape of the $I_c$-strain window; $I_c$ may show a peak value if there exist a residual strain induced in the tape during manufacturing. The difference of residual strain may be resulted from the adopted different deposition techniques. In this study, bending test of CC tapes has been done using the Goldacker bending test rig which can produce both compressive and tensile bending strain continuously or alternately to the sample. For SmBCO CC tapes, in continuous compressive bending test, $I_c$ showed a minimal increase and did not degrade up to the largest strain that can be applied using the bending rig equivalent to 1.15% based on the sample thickness. However, in the case of alternate application of compressive and tensile bending strain, $I_c$ showed a larger degradation and a lower reversible limit when compared with the case of continuous application of the bending strain. When $I_c$ started to degrade significantly at the tension side, the reversibility ended, also at the compression side which is resulted from the permanent deformation like delamination or cracks that was induced due to tensile bending strain.

• 한국결정학회:학술대회논문집
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• 한국결정학회 2007년도 추계학술연구발표회 및 정기총회
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• pp.23-23
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• 2007

• BMB Reports
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• 제47권4호
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• pp.184-191
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• 2014

Respiratory syncytial virus (RSV) is the leading cause of respiratory infection in infants and young children. Severe clinical manifestation of RSV infection is a bronchiolitis, which is common in infants under six months of age. Recently, RSV has been recognized as an important cause of respiratory infection in older populations with cardiovascular morbidity or immunocompromised patients. However, neither a vaccine nor an effective antiviral therapy is currently available. Moreover, the interaction between the host immune system and the RSV pathogen during an infection is not well understood. The innate immune system recognizes RSV through multiple mechanisms. The first innate immune RSV detectors are the pattern recognition receptors (PRRs), including toll-like receptors (TLRs), retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), and nucleotide-biding oligomerization domain (NOD)-like receptors (NLRs). The following is a review of studies associated with various PRRs that are responsible for RSV virion recognition and subsequent induction of the antiviral immune response during RSV infection.

• Molecules and Cells
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• 제42권10호
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• pp.721-728
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• 2019

Non-structural protein 1 (NS1) of influenza virus has been shown to inhibit the innate immune response by blocking the induction of interferon (IFN). In this study, we isolated two single-stranded RNA aptamers specific to NS1 with $K_d$ values of $1.62{\pm}0.30nM$ and $1.97{\pm}0.27nM$, respectively, using a systematic evolution of ligand by exponential enrichment (SELEX) procedure. The selected aptamers were able to inhibit the interaction of NS1 with tripartite motif-containing protein 25 (TRIM25), and suppression of NS1 enabled retinoic acid inducible gene I (RIG-I) to be ubiquitinated regularly by TRIM25. Additional luciferase reporter assay and quantitative real-time PCR (RT-PCR) experiments demonstrated that suppression of NS1 by the selected aptamers induced IFN production. It is noted that viral replication was also inhibited through IFN induction in the presence of the selected aptamers. These results suggest that the isolated aptamers are strongly expected to be new therapeutic agents against influenza infection.