• 미생물학회지
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• 제25권4호
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• pp.282-289
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• 1987

Pseudomonas putida KU816에서 분리한 플라스미드 pKU10의 여러가지 특성을 조사하고 그 제한 효소 지도를 작성하였다. pKU 10은 암펴설런, 테트라사이클린, 클로람페니콜에 대한 내성 유전자를 갖는 작은 R factor로서 마이토마이신 C에 의하여 큐어링 된다. 플라스미드의 크기는 9.4Kb로 측정되었다. pKU 10은 Pseudomonas와 E.coli블 숙주로 하였을 때 안정하게 형질 발현이 되다. 또한 pKU 10의 불화합성균은 IncP-I으로 조사되었다. Eco RI, Xho I. SaiI, BglII, SmaI은 pKU 10 DNA를 한 부위에서 자르고, Pst I은 두 부위, Hind Ill는 여섯 부위에서 자른다. 제한 효소 지도는 제한 효소를 이중, 삼중으로 완전 소화시키거나, 부분 소화시켜서 얻었다. pKU 10은 Pseudomonas속에서 유용한 클로닝 벡터호 이용된 것으로 기대된다.

• Journal of Biosystems Engineering
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• 제43권4호
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• pp.426-439
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• 2018

Purpose: To continuously monitor soil and climatic properties, a data acquisition system (DAQ) was developed and tested in plum farms (Gyewol-ri and Haechang-ri, Suncheon, Korea). Methods: The DAQ consisted of a Raspberry-Pi processor, a modem, and an ADC board with multiple sensors (soil moisture content (SEN0193), soil temperature (DS18B20), climatic temperature and humidity (DHT22), and rainfall gauge (TR-525M)). In the laboratory, various tests were conducted to calibrate SEN0193 at different soil moistures, soil temperatures, depths, and bulk densities. For performance comparison of the SEN0193 sensor, two commercial moisture sensors (SMS-BTA and WT-1000B) were tested in the field. The collected field data in Raspberry-Pi were transmitted and stored on a web server database through a commercial communications wireless network. Results: In laboratory tests, it was found that the SEN0193 sensor voltage reading increased significantly with an increase in soil bulk density. A linear calibration equation was developed between voltage and soil moisture content depending on the farm soil bulk density. In field tests, the SEN0193 sensor showed linearity (R = 0.76 and 0.73) between output voltage and moisture content; however, the other two sensors showed no linearity, indicating that site-specific calibration is important for accurate sensing. In the long-term monitoring results, it was observed that the measured climate temperature was almost the same as website information. Soil temperature information was higher than the values measured by DS18B20 during spring and summer. However, the local rainfall measured using TR 525M was significantly different from the values on the website. Conclusion: Based on the test results obtained using the developed monitoring system, it is thought that the measurement of various parameters using one device would be helpful in monitoring plum growth. Field data from the local farm monitoring system can be coupled with website information from the weather station and used more efficiently.

• 한국항행학회논문지
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• 제21권6호
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• pp.544-548
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• 2017

국제민간항공기구에서는 전 세계적으로 PBN 이행 촉구를 위해 2025년까지 모든 항공기의 SBAS 사용을 권고하고 있는 상황이다. 이에 국내에서도 한국형 SBAS인 KASS 개발이 진행 중에 있다. ICAO에서는 SBAS에 대한 인증 및 운용에 있어서 해당 항공 당국에 그 권한을 부여하고 있으며, KASS 또한 시스템 상세 설계, 제작, 설치 가 수행된 후 시스템에 대한 검증이 이루어 질 예정이다. 이에 본 논문에서는 국제 표준의 비행검사 규정을 통해 비행시험 파라미터를 도출 하였으며 이를 측정하기 위한 탑재측정장비의 구성방안에 대해서 제안 하였다.

• KSTLE International Journal
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• 제3권1호
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• pp.43-48
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• 2002

Dry sliding wear and friction test of CrN coaling on two types of aluminum alloy substrates,6061 Al and 7075 Al deposited by arc ion plating, was peformed with a ball-on-disk tribometer. The effects of normal Bead and the mechanical properties of substrate on the friction coefficient and wear-resistance of CrN coating were investigated. The worn surfaces were observed by SEM. The results show that surface micro-hardness of CrN- coated 7075 Al is higher than that of CrN-coated 6061 Al. With an increase in normal lead, wear volume increases, while the friction coefficient decreases. The friction coefficient of CrN-coated 6061 Al is higher than that of CrN-coated 7075 Al, while the wear-resistance of CrN-coated 6061 Al is lower than the CrN-coated 7075 Al's, which indicates that the substrate mechanical properties have strong inf1uences on the friction coefficient and wear of CrN coating. The main wear mechanism was fragments of CrN coating, which were caused by apparent plastic deformation of substrate during wear test.

• 한국항행학회논문지
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• 제19권1호
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• pp.1-6
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• 2015

전 세계적으로 급속하게 증가하는 항공교통량으로 인해 ICAO는 기존의 항행장비를 위성항법 기반으로 대체해 나가고 있으며, 이의 일환으로 기존의 ILS를 이용한 이착륙 서비스를 GBAS 로 대체할 계획을 수립 하였다. GBAS는 활주로까지 항공기를 유도하는 정밀 접근 서비스와 공항 주위의 정밀위치정보 서비스를 제공하는 위성항법 보강시스템을 이용하여 ILS와 달리 한 개의 시스템으로 활주로의 수에 관계없이 요구 성능을 제공할 수 있으며 curved approach가 가능하다는 장점을 보유하고 있다. 본 논문에서는 태안비행장 비행시험을 통한 ILS접근 절차와 GBAS curved approach절차를 비교하여 비행시간 단축 및 연료 감소효과를 분석하였으며 실제 비행시험을 통하여 이를 측정 확인 하였다.

• 한국치위생학회지
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• 제17권4호
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• pp.613-620
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• 2017

Objectives: The purpose of this study is to investigate the antibacterial effect of Streptococcus mutans of tea tree ingredient. Methods: The experimental groups were each given with different concentrations (30 or 50 vol%) of tea tree prepared in saline solution. The control group applied only saline solution. The tea tree coating of the specimen were examined under a scanning electron microscope. For the antibacterial activity test of the tea tree, the contact angle of the tea tree- coated specimen's surface was analyzed. The antibacterial effect against Streptococcus mutans was determined by counting the colony forming units (CFU). The statical statics were evaluated by using one-way ANOVA and paired t-test. Results: The tea tree treated group of hydrophilic more than non treated group. Antibacterial experiments demonstrated that tee tree solution was effective against Streptococcus mutans. However there was no significant difference in depending solution concentration groups. Conclusions: The antimicrobial activity of the tea tree containing solution showed its potential for use as coating for denture and medical materials.

• 복식문화연구
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• 제17권4호
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• pp.548-560
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• 2009

This study was conducted to investigate purchasing behavior of jeans consumers and to find differences in jeans evaluative criteria according to consumers' characteristics. Male and female university students participated in the study. Quota sampling method was used to collect the data. Data from 492 questionnaires were used for the statistical analysis. Descriptive analysis, factor analysis, Cronbach's alpha coefficient, ANOVA, Duncan test, and t-test were conducted. Three factors of jeans evaluative criteria classified (i.e., external criteria, internal/aesthetic criteria, internal/quality criteria). Results indicated that most of respondents put on jeans wear more than three times per a week and 38.1% of respondents reported that they owned three to four jeans products on average. Department store was the most used place to buy jeans product with 44.2%. 27.5% of respondents spent from 50,000 won to 100,000 won on purchasing jeans. Style was the highest important jeans evaluative criterion with 36.2%, followed by fitness, price, color, condition of washing treatment. Women respondents considered aesthetic criteria and quality criteria more importantly than men did. Respondents who were on a high income and spent more than 200,000 won per a month on apparel more concerned aesthetic criteria and external criteria than the other respondents did. Results of this study provide a basis for understanding jeans consumers' purchase behavior and evaluative criteria.

• 한국동물위생학회지
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• 제37권2호
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• pp.131-136
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• 2014

Thirteen diary cows were diagnosed as bovine tuberculosis (BTB) based on pathological findings and PCR method from 74 diary cows slaughtered in Incheon city. BTB was outbreaked 9 times in the farm from March 2010 to June 2013. The BTB confirmed cows were all negatives by intradermal test with PPD at 3 times. Recently, disagreement between BTB outbreak and intradermal test has been raised as a big problem. This phenomenon suggest that a new control programs are necessary at farm level as well as diagnostic level.

• 한국간호교육학회지
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• 제23권4호
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• pp.389-397
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• 2017

Purpose: This study was conducted to examine awareness of biomedical ethics, and to identify affecting factors of the awareness of biomedical ethics in nursing students. Methods: The subjects consisted of 266 nursing students their third and fourth years of study. The data were collected from October to December, 2015 by self-report using questionnaires. Data analysis was performed using SPSS/WIN 18.0, descriptive statistics, t-test, ANOVA, $Scheff{\acute{e}}$ test, Pearson correlation coefficient, and multiple regression analysis. Results: The mean score of the awareness of biomedical ethics was $2.81{\pm}0.22$, perception of death was $3.15{\pm}0.36$, and knowledge of brain death, organ donation, and organ transplant was $12.12{\pm}3.02$. The prediction factors of awareness of biomedical ethics were gender (${\beta}=.29$, p<.001), participation in religious activity (${\beta}=.23$, p=.015), and perception of death (${\beta}=.20$, p=.016). The explanation power was 17.1%. Conclusion: These results showed that education about biomedical ethics is necessary for nursing students, and the development of biomedical ethics educational programs should reflect affecting factors.

• 한국추진공학회지
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• 제14권2호
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• pp.63-70
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• 2010

본 연구에서는 액체로켓엔진 산화제로 과산화수소를 사용하기 위한 공급 설비 구축 및 세척 방법을 제시하고자 한다. 과산화수소를 추진제로 사용하기 위해서는 90% 이상의 고농도 과산화수소가 필요하며, 고농도 과산화수소의 공급 설비는 약간의 오염에도 민감하게 반응하는 과산화수소의 특성상 오염을 최소화해야 한다. 과산화수소 공급 설비의 오염을 최소화하기 위하여 공급 설비를 세척하고 세척된 공급 설비에 보호막을 입히는 표면안정화 절차를 확립하였다. 또한, 구축된 공급 설비는 누설 시험과 연소 시험 및 저장성 테스트를 통하여 안정성을 검증하였다.