• Molecules and Cells
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• 제42권5호
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• pp.406-417
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• 2019

RICE FLOWERING LOCUS T 1 (RFT1) is a major florigen that functions to induce reproductive development in the shoot apical meristem (SAM). To further our study of RFT1, we overexpressed the gene and examined the expression patterns of major regulatory genes during floral transition and inflorescence development. Overexpression induced extremely early flowering in the transgenics, and a majority of those calli directly formed spikelets with a few spikelets, thus bypassing normal vegetative development. FRUITFULL (FUL)-clade genes OsMADS14, OsMADS15, and OsMADS18 were highly induced in the RFT1-expressing meristems. OsMADS34 was also induced in the meristems. This indicated that RFT1 promotes the expression of major regulatory genes that are important for inflorescence development. RFT1 overexpression also induced SEPALLATA (SEP)-clade genes OsMADS1, OsMADS5, and OsMADS7 in the greening calli before floral transition occurred. This suggested their possible roles at the early reproductive stages. We found it interesting that expression of OsFD1 as well as OsFD2 and OsFD3 was strongly increased in the RFT1-expressing calli and spikelets. At a low frequency, those calli produced plants with a few leaves that generated a panicle with a small number of spikelets. In the transgenic leaves, the FUL-clade genes and OsMADS34 were induced, but SEP-clade gene expression was not increased. This indicated that OsMADS14, OsMADS15, OsMADS18, and OsMADS34 act immediately downstream of RFT1.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2485-2489
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• 2014

In the present study, we studied the hypermethylation of the human riboflavin transporter 2 (hRFT2) gene and regulation of protein expression in biopsies from resected tissues from Uighur cervical squamous cell carcinoma (CSCC) patients and their neighboring normal tissues. hRFT2 gene promoter region methylation sequences were mapped in cervical cancer cell line SiHa by bisulfite-sequencing PCR and quantitative detection of methylated DNA from 30 pairs of Uighur's CSCCs and adjacent normal tissues by MassARRAY (Sequenom, San Diego, CA, USA) and hRFT2 protein expression was analyzed by immunohistochemistry. In SiHa, we identified 2 CG sites methylated from all of 12CpG sites of the hRFT2 gene. Analysis of the data from quantitative analysis of single CpG site methylation by Sequenom MassARRAY platform showed that the methylation level between two CpG sites (CpG 2 and CpG 3) from CpG 1~12 showed significant differences between CSCC and neighboring normal tissues. However, the methylation level of whole target CpG fragments demonstrated no significant variation between CSCC ($0.476{\pm}0.020$) and neighboring normal tissues ($0.401{\pm}0.019$, p>0.05). There was a tendency for translocation the hRFT2 proteins from cytoplasm/membrane to nucleus in CSCC with increase in methylation of CpG 2 and CpG 3 in hRFT2gene promoter regions, which may relate to the genesis of CSCC. Our results suggested that epigenetic modifications are responsible for aberrant expression of the hRFT2 gene, and may help to understand mechanisms of cervical carcinogenesis.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제15권8호
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• pp.525-533
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• 2009

그리드 환경에서 작업에 필요한 파일은 스테이징 기법에 의해서 전송된다. 이때 요구되는 파일이 대용량일 경우 전송에 걸리는 시간이 늘어나 작업의 시작 시간을 지연시키게 되다. 또한, 이는 작업의 전체 실행 시간을 증가시키는 요인이 되어서 작업처리량을 감소시키는 결과를 가져오게 된다. 따라서 이러한 파일 전송 시간 때문에 생기는 오버헤드를 줄인다면 그리드에서 실행되는 작업의 효율을 상당히 향상 시킬 수 있다. 이러한 사실에 근거하여 본 논문에서는 그리드에서 효율적인 작업 처리를 위한 파일 프로비저닝 기법으로서 다음과 같은 두 가지 방법을 제안한다. 첫째, RA-RFT라는 방법을 제안한다. RA-RFT는 Globus Toolkit에서 파일 전송을 담당하는 RFT가 리플리카를 관리하는 RLS의 정보를 이용할 수 있도록 RFT를 확장한 것이다. RA-RFT는 파일 전송 시 가용한 리플리카들로부터 파일을 분할 전송함으로써 대용량 파일 전송 시간을 단축시킬 수 있다. 둘째 리모트 링크라는 방법을 제안한다. 리모트 링크는 파일을 계산 노드로 직접 전송하지 않고 계산 노드에서 파일에 원격 접근할 수 있는 방법을 제공한다. 원격 접근 방법을 이용함으로써 계산 노드의 저장 공간을 절약할 수 있고 선반입을 통해서 효율적인 파일 프로비저닝을 가능하게 한다. 우리는 이러한 두 가지 방법이 기존 그리드 환경에서 사용하고 있는 스테이징 방식보다 우월한 성능을 보여준다는 것을 다양한 실험을 통해서 증명한다.

• 방사선산업학회지
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• 제9권3호
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• pp.119-126
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• 2015

In Shinjeong-dong of Jeongeup, there are three government-supported research institutes and an RFT industrial complex which is currently being established. Increased human activities can affect flora and fauna as a man-made pressure onto the region. As a baseline study, status of exotic plants was investigated prior to a full operation of the RFT industrial complex. A total of 54 species and 1 variety of naturalized or introduced plants were found in the study area. Among them, three species (Ambrosia artemisifolia var. elatior, Rumex acetocella and Aster pilosus) belong to 'nuisance species', and four species(Phytolacca americana, Iopomoea hederacea, Ereechtites hieracifolia and Rudbeckia laciniata) to 'monitor species' designated by the ministry of Environment. Some of naturalized trees and plants were intentionally introduced in this area, while others naturally immigrated. Physalis angulata seemes to immigrate in the study area in the form of mixture with animal feeds as its distribution coincided with the transportation route of the animal feeds. Liquidambar styraciflua is amenable to the ecological investigation on the possible expansion of the species to the nearby Naejang National Park as its leave shape and autumn color are very similar to those of maple trees. The number of naturalized plants around the RFT industrial complex will increase with an increase in floating population, in human activities in association with constructions of factories and operations of the complex. The result of this study provides baseline data for assessing the ecological change of the region according to the operation of the RFT industrial complex.

• Journal of Acupuncture Research
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• 제17권2호
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• pp.11-18
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• 2000

Bee-venom Acupucture has good effect on pain control but We may be anxious about the problem of Liver and Kidneys on many times treated patients. Therefore, In order to analysis the clinical form, We have observed the LFT, RFT of 37 patients who visited Won-Kwang University Kunpo Oriental Medical Hospital and treated Bee Venom therapy over 20 times from March 1999 to February 2000. The results were summarized as follows 1. The distribution of sex was 25 females, 12 males, and the average of patients age was 42.3 years 2. The distribution of disease was degenerative arthritis, HIVD of L-spine, HIVD of C-spine, spinal stenosis, RA, etc. 3. The total treated times is 1464 and the average of treated times is 39.6 times 4. The results of the LFT(AST, ALT) of 37 patients showed that normal impression is occupied 91.9% and abnormal impression is occupied 8.1%. 5. The results of the RFT(BLTN, Creatinine) of 37 patients showed that normal impression is occupied 100%. 6. The results of treatment using BV-acupunture showed that 81.1% achieved above excelent recovery 13.5% achieved above good recovery, 5.4% achieved fair recovery.

• 원자력산업
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• 제34권2호
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• pp.52-55
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• 2014

• 섬유기술과 산업
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• 제5권1_2호
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• pp.35-52
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• 2001

• Journal of Korean Neurosurgical Society
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• 제50권5호
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• pp.446-452
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• 2011

Objective : We retrospectively investigated the long-term results of percutaneous radiofrequency thermocoagulation (RFT) using fluoroscopic image-guidance for treatment of trigeminal neuralgia. Methods : A total of 38 patients diagnosed and treated with RFT as an idiopathic trigeminal neuralgia were investigated. To minimize the risks related to conventional technique based on cutaneous landmarks, and to eliminate the need to frequent reposition of cannula, we adopted a technique of image-guided fluoroscopic cannulation of the foramen ovale. To minimize sensory complication following thermal lesion, our target response was a generation of a lesion with mild to moderate hypalgesia rather than dense hypalgesia. Results : The immediate pain-relief was achieved in all patients underwent RFT. With mean duration of follow-up of 38.2 months (range,12-72), 11 (28.9%) experienced recurrence of pain. The mean timing of recurrence was 26.1 months (range,12-46). A 42.7% recurrence rate was estimated by Kaplan-Meier analysis for the 38 patients at 46 months; 20.2% within 2 years, 29.1% within 3 years. In the long-term, 27 patients (71%) and 6 patients (15.8%) showed Barrow Neurological Institute (BNI) score I and BNI score II responses. Three (7.9%) patients was assessed as BNI score III, 2 patients (5.3%) showed BNI score IV response. As a complication, troublesome dysesthesia occurred in 3 of 38 patients (7.9%), however, there was no permanent cranial nerve palsy or morbidity. Conclusion : These results indicates that RFT under fluoroscopic image-guided cannulation of foramen ovale is a safe, effective, and reliable means of treating trigeminal neuralgia.

• 동의생리병리학회지
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• 제22권6호
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• pp.1585-1588
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• 2008

The purpose of this study was to investigate the clinical application of oriental medical therapy(OMT) to Speech retardation complained problems of Articulation & Reading fluency. We treated the patient with OMT & others. The recovery of Speech retardation was evaluated by Articulation correction test(ACT) & Reading fluency test(RFT). The applicability of OMT & other therapy has positive effects on the patient with Speech retardation complained problems of Articulation & Reading fluency. The scores of ACT & RFT were increased.

• 대한약침학회지
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• 제13권2호
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• pp.67-73
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• 2010

Objective : The purpose of this study is to determine the efficacy and safely of Hwangryunhaedok-tang Pharmacopuncture (HHT-PA) therapy on post-stroke depression (PSD) in stroke patients. Methods : Fifteen PSD patients has been recruited and evaluated with liver function test (LFT) and renal function lest (RFT) and depression scales, Beck Depress ion Inventory (BDI) and Hamilton Depression Rating Scale (HAM-D) before and after HHT-PA treatment. Results : HAM-D score significantly decreased after HHT-PA treatment regard less of improving of motor function. But BDI score did not decrease significantly. All results of LFT and RFT were in normal range before and after HHT-PA treatment Conclusion : These results showed that HHT-PA decrease PSD symptoms and has safety on stroke patients, although has no effect on improving of motor impairment.