• Title/Summary/Keyword: RFB

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Acinetobacter calcoaceticus Glucose-1-phosphate Thymidylyltransferase: Cloning, Sequencing, and Expression in E.coli

  • Eun, Suk-Ho;Kim, Dae-Jin;Kim, Yu-Sam
    • BMB Reports
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    • v.34 no.3
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    • pp.230-236
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    • 2001
  • dTDP-rhamnose is synthesized from dTTP and glucose-1-phosphate by four enzymatic steps in the gram-negative bacteria. By using a homologous PCR product, a gene cluster encoding four genes (rfbA, rfbB, rfbC, rfbD) involved in L-rhamnose biosynthesis by Acinetobacter calcoaceticus was isolated and sequenced. The four genes were clustered on the biosynthetic operon in the order of rfbB, D, A, C. A gene, rfbA, encoding glucose-l-phosphate thymidylyltransferase (RfbA), was cloned from A. calcoaceticus pathogenic and encapsulated in the gram-negative bacterium. This enzyme catalyzes the formation of dTDP-D-glucose From $\alpha$-D-glucose-1-phosphate and dTTP.RfbA was amplified by PCR and inserted into the $T_7$ expression system. The activity of RfbA was determined by the capillary electrophoresis. The $K_m$ values for dTTP and $\alpha$-D-glucose-1-phosphate were calculated to be 1.27 mM and 0.80 mM, respectively by using the Line-Weaver Burk plot. RfbA is inactivated by diethylpyrocarbonate.

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Electrochemical Properties of Current Collector in the All-vanadium Redox Flow Battery (바나듐 레독스-흐름 전지에서 집전체의 전기화학적 특성)

  • Hwang, Gan-Jin;Oh, Yong-Hwan;Ryu, Cheol-Hwi;Choi, Ho-Sang
    • Korean Chemical Engineering Research
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    • v.52 no.2
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    • pp.182-186
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    • 2014
  • Two commercial carbon plates were evaluated as a current collector (bipolar plate) in the all vanadium redox-flow battery (V-RFB). The performance properties of V-RFB were test in the current density of $60mA/cm^2$. The electromotive forces (OCV at SOC 100%) of V-RFB using A and B current collector were 1.47 V and 1.54 V. The cell resistance of V-RFB using A current collector was $4.44{\sim}5.00{\Omega}{\cdot}cm^2$ and $3.28{\sim}3.75{\Omega}{\cdot}cm^2$ for charge and discharge, respectively. The cell resistance of V-RFB using B current collector was $4.19{\sim}4.42{\Omega}{\cdot}cm^2$ and $4.71{\sim}5.49{\Omega}{\cdot}cm^2$ for charge and discharge, respectively. The performance of V-RFB using each current collector was evaluated. The performance of V-RFB using A current collector was 93.1%, 76.8% and 71.4% for average current efficiency, average voltage efficiency and average energy efficiency, respectively. The performance of V-RFB using B current collector was 96.4%, 73.6% and 71.0% for average current efficiency, average voltage efficiency and average energy efficiency, respectively.

Research Review of the All Vanadium Redox-flow Battery for Large Scale Power Storage (대용량 전력저장용 바나듐 레독스-흐름 전지 연구동향)

  • Choi, Ho-Sang;Kim, Jae-Chul;Ryu, Cheol-Hwi;Hwang, Gab-Jin
    • Membrane Journal
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    • v.21 no.2
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    • pp.107-117
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    • 2011
  • The all vanadium redox-flow battery (V-RFB) is investigating as one of large-scale power storage systems. Particularly, V-RFB is being investigated as one of the power storage systems for the load leveling and output power equalization of the power systems using renewable energy such as solar and wind. In this paper, it was explained for the principle and construction, recent research review, economy, element technology in V-RFB.

Design and Test of ESS DC-DC Converter using Zinc-Bromine Redox Flow Battery for Stand-alone Microgrid (Zinc-Bromine 레독스 플로우 배터리를 이용한 독립형 마이크로그리드 ESS DC-DC 컨버터 설계 및 실증)

  • Choe, Jung-Muk;Ra, Sun-Gil;Han, Dong-Hwa;Lee, Yong-Jin;Choe, Gyu-Ha
    • The Transactions of the Korean Institute of Power Electronics
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    • v.19 no.2
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    • pp.106-115
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    • 2014
  • This paper proposes ESS DC-DC Converter using Redox Flow Battery (RFB) for stand-alone microgrid. Price, safety, expandability and dynamics are crucial in ESS. Reports show that Zinc-bromine (ZnBr) RFB is the best choice in ESS. Simple electrical ZnBr RFB model is obtained from charging test. DC-DC converter Inductor current-DClink Voltage model is proposed for the DC microgrid. For the controller design in z-domain, the K-factor method is by considering nature of the digital controller. The control performance has been verified with simulation and hardware experiments. Lastly 10kW DC microgrid using RFB test result is shown.

Durability of Cation Exchange Membrane Containing Psf (polysulfone) in the All-vanadium Redox Flow Battery (Psf (polysulfone) 함유 양이온교환막의 바나듐 레독스-흐름 전지에서의 내구성)

  • Kim, Joeng-Geun;Kim, Jae-Chul;Ryu, Cheol-Hwi;Hwang, Gab-Jin
    • Membrane Journal
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    • v.21 no.2
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    • pp.141-147
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    • 2011
  • The cation exchange membrane using TPA (tungstophosphoric acid) and the block co-polymer of polysulfone and polyphenylenesulfidesulfone was prepared for a separator of all-vanadium redox flow battery. The membrane resistance of the prepared cation exchange membrane in 1mol/L $H_2SO_4$ aqueous solution was measured. The membrane resistance of the prepared Psf-PPSS and Psf-TPA-PPSS cation exchange membrane was about $0.94{\Omega}{\cdot}cm^2$. Electrochemical property of all-vanadium redox flow battery using the prepared cation exchange membrane was measured. The measured charge-discharge cell resistance of V-RFB at 4 A decreased in the order; Nafion117 < Psf-TPA-PPSS < Psf-PPSS. The durability of membrane was earried out by soaking it in $VO_2{^+}$ solution and evaluated by measuring the charge-discharge cell resistance of V-RFB with an increase of soaking time. The prepared Psf-PPSS cation exchange membrane had high durability and Psf-TPA-PPSS cation exchange membrane had almost same durability compared with Nafion117.

Characteristics of Poly(arylene ether sulfone) Membrane for Vanadium Redox Flow Battery (바나듐 레독스 흐름전지용 Poly(arylene ether sulfone) 막의 특성)

  • Oh, Sung-June;Jeong, Jae-Hyeon;Shin, Yong-Cheol;Lee, Moo-Seok;Lee, Dong-Hoon;Chu, Cheun-Ho;Kim, Young-Sook;Park, Kwon-Pil
    • Korean Chemical Engineering Research
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    • v.51 no.6
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    • pp.671-676
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    • 2013
  • Recently, there are many efforts focused on development of Redox Flow Battery (RFB) for large energy storage system. Economical hydrocarbon membranes alternative to fluorinated membranes for RFB membrane are receiving attention. In this study, characteristics of poly(arylene ether sulfone) (PAES) were compared with expensive fluorinated membrane at VRB (Vanadium Redox Flow Battery) operation condition. Permeability of vanadium ion through membrane, ion exchange capacity (IEC), change of OCV, swelling, charge-discharge curves and energy efficiency were measured. PAES membrane showed lower permeability of vanadium ion, higher IEC and then higher energy efficiency compared with Nafion 117 membranes.

Extending VNC Server and Client for Sharing Clipboard Contents Composed of Text and Images (텍스트와 이미지로 구성된 클립보드 콘텐츠 공유를 위한 VNC 서버와 클라이언트의 확장)

  • Lee, Tae-Ho;Lee, Hong-Chang;Park, Yang-Su;Lee, Myung-Joon
    • Journal of the Korea Society of Computer and Information
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    • v.13 no.4
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    • pp.115-126
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    • 2008
  • VNC(Virtual Network Computing) is a desktop sharing system based on the RFB(Remote Framebuffer) protocol which allows you to control a remote computer running a VNC server through a VNC client(or viewer) on a local computer. To exchange information between the two computers, VNC provides the functionality of sharing the clipboard contents. Unfortunately, the current VNC softwares support only the clipboard text contents, not providing methods for sharing the clipboard multimedia contents such as images. In this paper, we extend the RFB protocol to share the clipboard contents composed of text and images. Also, to support the developed protocol. we extend both the UltraVNC server and the JavaViewer VNC client which are free open-source softwares. Through the developed VNC softwares, users can exchange the clipboard contents including texts and images between the remote computer and the local computer.

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Whitening and Antioxidative Effect of Rice Bran Fermented by Bacillus subtilis (발효된 미강의 항산화 효과 및 미백 효과)

  • Chae, Ga-Yeon;Kwon, Ryun-Hee;Jang, Min-Woo;Kim, Min-Jung;Ha, Bae-Jin
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.37 no.2
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    • pp.153-159
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    • 2011
  • In this study, we investigated rice bran fermented by Bacillus subtilis (RFB) at $40^{\circ}C$ for 36 h to develop a new natural antioxidant and whitening agent for new natural cosmetics. RFB showed reactive oxygen species (ROS) scavenging activities in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical scavenging activities and hydroxyl radical scavenging activities at a concentration of $100 \;{\mu}L$/mL. DPPH, superoxide radical scavenging activities and hydroxyl radical scavenging activities were higher in the RFB than in the RB. Inhibitory activity on auto-oxidation of linoleic acid is also highest at a concentration of $100 \;{\mu}L$/mL. RFB showed the higher inhibitory activity than RB in auto-oxidation. RFB reduced intracellular tyrosinase activity about 80 % at a concentration of $100 \;{\mu}L$/mL. Therefore, we suggest that RFB could be used as a useful antioxidant and whitening agent.

A Study on the Electrochemical Performance of Fe-V Chloric/Sulfuric Mixed Acid Redox Flow Battery Depending on Electrode Activation Temperature (Fe-V Chloric/Sulfuric Mixed Acid 레독스흐름전지 전극의 활성화 온도에 따른 전기화학적 성능 고찰)

  • Lee, Han Eol;Kim, Dae Eop;Kim, Cheol Joong;Kim, Taekeun
    • Applied Chemistry for Engineering
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    • v.31 no.6
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    • pp.639-645
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    • 2020
  • Among the components of redox flow battery (RFB), the electrode serves as a diffusion layer of an electrolyte and a path for electrons and also is a major component that directly affects the RFB performance. In this paper, chloric/sulfuric mixed acidwas used as a supporting electrolyte in RFB system with Fe2+/Fe3+ and V2+/V3+ as redox couple. The optimum electrode and activation temperature were suggested by comparing the capacity, coulombic efficiency and energy efficiency according to the electrode type and activation temperature. In the RFB single cell evaluation using 5 types of carbon electrodes used in the experiments, all of them showed close to the theoretical capacity to retain the reliability of the evaluation results. GFD4EA showed relatively excellent energy efficiency and charge/discharge capacity. In order to investigate the electrochemical performance according to the activation temperature, GFD4EA electrode was activated by heat treatment at different temperatures of 400, 450, 500, 600 and 700 ℃ under an air atmosphere. Changes in physical properties before and after the activation were observed using electrode mass retention, scanning electron microscope (SEM), XPS analysis, and electrochemical performance was compared by conducting RFB single evaluation using electrodes activated at each temperature given above.

Apple Detection Algorithm based on an Improved SSD (개선 된 SSD 기반 사과 감지 알고리즘)

  • Ding, Xilong;Li, Qiutan;Wang, Xufei;Chen, Le;Son, Jinku;Song, Jeong-Young
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.3
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    • pp.81-89
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    • 2021
  • Under natural conditions, Apple detection has the problems of occlusion and small object detection difficulties. This paper proposes an improved model based on SSD. The SSD backbone network VGG16 is replaced with the ResNet50 network model, and the receptive field structure RFB structure is introduced. The RFB model amplifies the feature information of small objects and improves the detection accuracy of small objects. Combined with the attention mechanism (SE) to filter out the information that needs to be retained, the semantic information of the detection objectis enhanced. An improved SSD algorithm is trained on the VOC2007 data set. Compared with SSD, the improved algorithm has increased the accuracy of occlusion and small object detection by 3.4% and 3.9%. The algorithm has improved the false detection rate and missed detection rate. The improved algorithm proposed in this paper has higher efficiency.