• Herbal Formula Science
• /
• v.28 no.1
• /
• pp.29-39
• /
• 2020

Objectives : Glucoraphanin is one of the well-known natural glucosinolates found in cruciferous plants. In the present study, we investigated the effects and molecular mechanism of glucoraphanin in dexamethasone-induced skeletal muscle atrophy in vitro model. Methods : The cytotoxic effects of glucoraphanin on C2C12 myoblasts or myotubes were evaluated by MTT assay. The glucoraphanin was evaluated effects in dexamethasone-induced skeletal muscle atrophy in C2C12 myotubes using a real-time PCR, western blots analysis, and immunofluorescence staining of myosin heavy chain. Result : Glucoraphanin had no cytotoxicity on both C2C12 myoblasts or myotubes. Dexamethasone markedly induced muscle atrophy by up-regulating muscle-specific ubiquitin E3 ligase markers, atrogin-1 and MuRF1, and down-regulating MyoD, a myogenic regulatory factor whereas co-treatment of glucoraphanin and dexamethasone dose-dependently inhibited it. Furthermore, decreased expressions of p-Akt, p-FOXO1, and p-FOXO3a induced by dexamethasone were reversed by co-treatment with glucoraphanin and dexamethasone. In addition, dexamethasone obviously reduced myotube diameters, while co-treatment of glucoraphanin and dexamethasone increased those to a similar level as control. Conclusions : These results show that glucoraphanin suppresses dexamethasone-induced muscle atrophy in C2C12 myotubes through activation of Akt/FOXO signaling pathway.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 1999.10a
• /
• pp.56-56
• /
• 1999

For advanced TFT-LCD manufacturing processes, dry etching of thin-film layers(a-Si, $SiN_x$, SID & gate electrodes, ITO etc.) is increasingly preferred instead of conventional wet etching processes. To dry etch Al gate electrode which is advantageous for reducing propagation delay time of scan signals, high etch rate, slope angle control, and etch uniformity are required. For the Al gate electrode, some metals such as Ti and Nd are added in Al to prevent hillocks during post-annealing processes in addition to gaining low-resistivity($<10u{\Omega}{\cdot}cm$), high performance to heat tolerance and corrosion tolerance of Al thin films. In the case of AI-Nd alloy films, however, low etch rate and poor selectivity over photoresist are remained as a problem. In this study, to enhance the etch rates together with etch uniformity of AI-Nd alloys, magnetized inductively coupled plasma(MICP) have been used instead of conventional ICP and the effects of various magnets and processes conditions have been studied. MICP was consisted of fourteen pairs of permanent magnets arranged along the inside of chamber wall and also a Helmholtz type axial electromagnets was located outside the chamber. Gas combinations of $Cl_2,{\;}BCl_3$, and HBr were used with pressures between 5mTorr and 30mTorr, rf-bias voltages from -50Vto -200V, and inductive powers from 400W to 800W. In the case of $Cl_2/BCl_3$ plasma chemistry, the etch rate of AI-Nd films and etch selectivity over photoresist increased with $BCl_3$ rich etch chemistries for both with and without the magnets. The highest etch rate of $1,000{\AA}/min$, however, could be obtained with the magnets(both the multi-dipole magnets and the electromagnets). Under an optimized electromagnetic strength, etch uniformity of less than 5% also could be obtained under the above conditions.

• The Korean Journal of Physiology and Pharmacology
• /
• v.22 no.4
• /
• pp.427-436
• /
• 2018

The objective of this study was to analyze the concurrent treatment effects of ursolic acid (UA) and low-intensity treadmill exercise and to confirm the effectiveness of UA as an exercise mimetic to safely improve muscle atrophy-related diseases using Sprague-Dawley (SD) rats with skeletal muscle atrophy. Significant muscle atrophy was induced in male SD rats through hind limb immobilization using casting for 10 days. The muscle atrophy-induced SD rats were group into four: SED, sedentary; UA, daily intraperitoneal UA injection, 5 mg/kg; EX, low-intensity (10-12 m/min, $0^{\circ}$ grade) treadmill exercise; and UEX, daily intraperitoneal UA injection, 5 mg/kg, and low-intensity (10-12 m/min, $0^{\circ}$ grade) treadmill exercise. After 8 weeks of treatment, endurance capacity was analyzed using a treadmill, and tissues were extracted for analysis of visceral fat mass, body weight, muscle mass, expression of muscle atrophy- and hypertrophy-related genes, and endurance capacity. Although the effects of body weight gain control, muscle mass increase, and endurance capacity improvement were inadequate in the UA group, significant results were confirmed in the UEX group. The UEX group had significantly reduced body weight and visceral fat, significantly improved mass of tibialis anterior and gastrocnemius muscles, and significantly decreased atrophy-related gene expression of MuRF1 and atrogin-1, but did not have significant change in hypertrophy-related gene expression of Akt and mTOR. The endurance capacity was significantly improved in the EX and UEX groups. These data suggest that concurrent treatment with low-intensity exercise and UA is effective for atrophy-related physical dysfunctions.

• The Journal of Korean Physical Therapy
• /
• v.27 no.4
• /
• pp.207-213
• /
• 2015

Purpose: The aim of the current study was to investigate EMG activity on dynamic balance of subjects with functional lumbar instability following fatigue of low back. Methods: The subjects (24 university students) were divided into 2 groups; functional lumbar instability group (6 males and 6 females) and lumbar stable group (7 males and 5 females) who could complete a questionnaire and undergo a prone instability test. All participants were evaluated for distribution of muscle activity using the TeleMyo DTSTM system. Dynamic balance was tested by Y balance test. This study was conducted for measurement of EMG activity on dynamic balance with the difference between FLIG and control group following muscle fatigue. Results: The functional lumbar instability group (FLIG) showed a significantly lower YBT score (%) of anterior, posterolateral direction on Y-balance test (YBT) in dynamic balance than the lumbar stable group (LSG) (p<0.05). The FLIG was significantly lower than the LSG in anterior direction in EMG activity(%) of MF, RA, ES, GMX, GME, RF, and posteromedial direction in EMG activity(%) of IO, ES and then posterolateral direction in EMG activity(%) of IO, ES in dynamic balance (p<0.05). There was significant correlation of MF, RA, and GMX in anterior reach direction (p<0.05) and ES, GME (p<0.01) and IO, ES in posteromedial reach direction (p<0.05) and EO, ES, GMX in posterolateral reach direction (p<0.05) there was positive correlation. Conclusion: This study showed that FLIG effected EMG activity by dynamic balance following muscle fatigue. Further study is needed for measurement of various ages and work with lumbar instability for clinical application.

• Korean Journal of Applied Biomechanics
• /
• v.17 no.4
• /
• pp.141-148
• /
• 2007

Falls associated with tripping over an obstacle can be dangerous, yet little is known about the strategies used for stepping over obstacles in older Parkinson disease. The purpose of this study was to investigate the lower extremity muscle activity on the obstacle gait according to obstacle height in older Parkinson diseases. The obstacle gait of 7 older Parkinson disease was examined during a 5.0 m approach to, and while stepping over, obstacles of 0, 25, 52, and 152mm. Seven pairs of surface electrodes(Noraxon MyoResearch, USA) were attached to the right-hand side of the body to monitor the adductor longus(AL), gluteus medius(GME), gluteus maximus(GMA), biceps femoris(BF), rectus femoris(RF), gastrocnemius(GA), tibialis anterior(TA). Electromyography data were filtered using a 10Hz to 350 Hz Butterworth band-pass digital filter and normalized to the maximum value in the analyzed phases. A one-way ANOVA for repeated measures was employed for selected electromyography variables to analyze the differences of the height of four obstacles. The results showed significant differences between 0.0mm and 25, 52, and 152mm obstacle height in TA and GA activities during the second phase(swing phase). But the more increase obstacle height, the more not increase the muscle activities. This means that the Parkinson disease stepping over obstacle inefficiency. To prevent and reduce the frequency of falls, elderly Parkinson disease maintained and improved their balance, muscular strength, neuromuscular control and mobility.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2012.02a
• /
• pp.523-523
• /
• 2012

As the wafer geometric requirements continuously complicated and minutes in tens of nanometers, the expectation of real-time add-on sensors for in-situ plasma process monitoring is rapidly increasing. Various industry applications, utilizing plasma impedance monitor (PIM) and optical emission spectroscopy (OES), on etch end point detection, etch chemistry investigation, health monitoring, fault detection and classification, and advanced process control are good examples. However, process monitoring in semiconductor manufacturing industry requires non-invasiveness. The hypothesis behind the optical monitoring of plasma induced ion current is for the monitoring of plasma induced charging damage in non-invasive optical way. In plasma dielectric via etching, the bombardment of reactive ions on exposed conductor patterns may induce electrical current. Induced electrical charge can further flow down to device level, and accumulated charges in the consecutive plasma processes during back-end metallization can create plasma induced charging damage to shift the threshold voltage of device. As a preliminary research for the hypothesis, we performed two phases experiment to measure the plasma induced current in etch environmental condition. We fabricated electrical test circuits to convert induced current to flickering frequency of LED output, and the flickering frequency was measured by high speed optical plasma monitoring system (OPMS) in 10 kHz. Current-frequency calibration was done in offline by applying stepwise current increase while LED flickering was measured. Once the performance of the test circuits was evaluated, a metal pad for collecting ion bombardment during plasma etch condition was placed inside etch chamber, and the LED output frequency was measured in real-time. It was successful to acquire high speed optical emission data acquisition in 10 kHz. Offline measurement with the test circuitry was satisfactory, and we are continuously investigating the potential of real-time in-situ plasma induce current measurement via OPMS.

• Current Photovoltaic Research
• /
• v.3 no.3
• /
• pp.80-84
• /
• 2015

We suggest new emitter formation method using solid-phase epitaxy (SPE); solid-phase epitaxy emitter (SEE). This method expect simplification and cost reduction of process compared with furnace process (POCl3 or BBr3). The solid-phase epitaxy emitter (SEE) deposited a-Si:H layer by radio-frequency plasma-enhanced chemical vapor deposition (RF-PECVD) on substrate (c-Si), then thin layer growth solid-phase epitaxy (SPE) using rapid thermal process (RTP). This is possible in various emitter profile formation through dopant gas ($PH_3$) control at deposited a-Si:H layer. We fabricated solar cell to apply solid-phase epitaxy emitter (SEE). Its performance have an effect on crystallinity of phase transition layer (a-Si to c-Si). We confirmed crystallinity of this with a-Si:H layer thickness and annealing temperature by using raman spectroscopy, spectroscopic ellipsometry and transmission electron microscope. The crystallinity is excellent as the thickness of a-Si layer is thin (~50 nm) and annealing temperature is high (<$900^{\circ}C$). We fabricated a 16.7% solid-phase epitaxy emitter (SEE) cell. We anticipate its performance improvement applying thin tunnel oxide (<2nm).

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.23 no.9A
• /
• pp.2153-2164
• /
• 1998

This paper has presented the parameters for the coexistence between two systems in macro/microcell ovelaid W-CDMA WLL (wideband CDMA wireless local loop) and has calculated the capacity of forward/reverse link in microcell. To produce the capacity for analyzing system interference effects, we have shown tables and graphs with the parameters sucyh as RF channel bandwidth of WLL(W), the transmission rate of service message(R), the required signal power to noise power ratio( $E_{b/}$ $N_{0}$) for achieving accepatable error rate, te user number ( $N_{W1}$, $N_{W2}$) of the neighboring system, the signal power to interference power ratio(.GAMMA.$_{C1B}$, .GAMMA.$_{C2B}$) of the neighboring system, the normalized distance(d) between microcell and macrocell base-station, and microcell to macrocell radius ratio ( $R_{d}$). From the results, we have convinced that the capacity of microcell diminishes as increasing the user number ( $N_{W2}$) in macrocell, increasing the microcell radius, and decreasing the normalized distance(d) between microcell and macrocell base-station. Especially, we have known that when $R_{d}$=0.1, $N_{W2}$ must be below 24 at .GAMMA.$_{C2B}$ = 0 dB and below 8 at .GAMMA.$_{C2B}$ = 4 dB for the acceptable capacity raito to be over 80%. Therfore, this paper is usefult to design microcell W-CDMA WLL for accommodating more user number under the interference effects of macrocell W-CDMA WLL and is expected to be reference in power control if base-station.ation.ion.ation.ation.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.28 no.6
• /
• pp.478-486
• /
• 2017

In this paper, we propose an algorithm for the wideband beamforming in a conformal phased array antenna by compensating the errors. For the wideband beamforming, we used the True Time Delay(TTD), which was fabricated on the RF circuit board to obtain long delay lines. Beamforming errors in the conformal array antenna are the mutual coupling between the array elements, the dispersive error in the TTD circuit, and the quantization error by the digital control. We apply the compensation algorithm to the conformal phased array antenna of wideband 2~4 GHz, and verify the usefulness by comparing the results with the experiment results.

• The Korean Journal of Zoology
• /
• v.39 no.1
• /
• pp.75-88
• /
• 1996

OTF9-63 (OTF9) and P19S1O1A1 (P19) embryonal carcinoma (EC) cells were examined for their ability to produce the readivation of inactive X chromosomes from somatic cells. They were hybridized with various somatic cells and resulting HATr EC-somatic cell clones were analysed for their morphology, chromosomal replication pafterns and expression proffies of X-linked and distantiy located genes, Hprt and Pgk-1. The results demonstrated that 0RF9 cells could reactivate the inactive X chromosome whereas P19 cells could not. In adition, EC-somatic cell hybrids tended to reduce the number of sex chromosomes in long-term culture, resulting m 1:2 ratio of sex chromosomes to autosomes The use of EC cell hybrids provides an experimental system for studying the mechanism(s) of the X-reactivatio that is initiated and maintained from meiotic prophase of oogenesis to early embryogenesis.