• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.2
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• pp.203-211
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• 2013

Selected 11 rice transgenic lines, through preliminary yield trials on 32 lines, were evaluated on important agronomic traits as well as grain quality by conducting replicated yield trials having three replication plots. Japonica recipients, Nipponbare, Nagdongbyeo, and Dongjinbyeo, were the recipients of 7 transformed genes, which were putatively related with high yield, abiotic stress tolerance, and disease resistance. To estimate the degrees of deviations from the wild types, 11 traits of transgenic lines, relating with grain quality were evaluated and subjected to multivariate analyses. Principal coordinate and clustering analyses did not support collection manners of transgenic lines in terms of the genes transformed as well as the genetic background. Meanwhile, some transgenic lines would be acceptable due to their over-all performances were similar to their wild types, it was hardly possible to declare any transgenic line, which adhered closely to the commercial profits of wild type. Thereby, with considerations on the demanding resources in establishing rice transgenic lines having market competitiveness, it was speculated that proper application of breeding strategies would be crucial factor for the efficiency of developing prospective rice transgenic lines.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.6
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• pp.419-424
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• 2008

Objective of this study was to evaluate the adaptation and the soil conservation effect of improved wild vine in sloped upland of highland about 600m from sea level in Korea. A mainly producing district of the improved wild vine in Korea was distributed 230m to 540m from sea level, and its rainfall ranged 1,200 to 1,700 mm. The lowest temperature was from -19.6 to $-25.4^{\circ}C$. Their soil texture was from sandy loam to clay loam, where drainage class was well drained and the contents of soil organic matter and total nitrogen in the improved wild vine's cultivation area were higher than those of Chinese cabbage's cultivation area in the highland. According to improved wild vine's cultivation methods, the amounts of soil erosion were 25.5,4.1, and $1.8MT\;ha^{-1}$ in clean culture, part sod + mulching, and nature sod, respectively. Those were below 30 to $80MT\;ha^{-1}$ of sloped upland at highland in Korea. The result suggests that it is possible to cultivate the improved wild vine over wintering at 600m of highland above the sea level. We can substitute the improved wild vine for Chinese cabbage in the highland for soil conservation.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.1-8
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• 2008

Four cover plants such as Phlox subulata, Glechoma hederacea var. longituba, Sedum middendorffianum and Saxifraga laciniata were evaluated to investigate the effect of companion planting on reducing soil erosion in Chinese cabbage cultivated highland. The experiment was conducted using lysimeters of $5\;m{\times}2\;m$ (length$\times$width) with 5, 15 and 30% slopes. Companion plants except Sedum middendorffianum did not interface with growth of Chinese cabbage Glechoma hederacea var. longituba and Sedum middendorffianum grew faster than Phlox subulata and Saxifraga laciniata in the early growth stage suer transplanting, resulting in fast ground covering, but the ground covering by Phlox subulata and Saxifraga laciniata was delayed because growth suppression by high air temperature during summer season. Soil erosion became severe as increasing degree of slope. Assessments of the four cover plants were conducted in relation to soil conservation characteristic of scenery, endurance to the environment stress, plant growth and weed suppression. From the assessments, Phlox subulata was superior to other intercropping crops tested for reducing soil erosion in highland cultivation of Chinese cabbage.

• Korean Journal of Poultry Science
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• v.32 no.3
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• pp.179-186
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• 2005

This study was conducted to establish a comprehensive guideline for grading system of poultry meat cuts. Slaughter and further processed houses were divided into large(>70,000 heads/day), middle($50,000\~25,000$ heads/day), and small scale(<25,000 heads/day), and the production managers were asked by the Questionnaire. Most poultry meat cuts produced by larger- and middle-slaughter house were consumed by dealers, franchisee and department store in increasing order, whereas further processed poultry meat cuts(small scale) provided meals for the students in the high amount. The packaging step was best f3r the determination of grading in the large slaughter house. This survey indicated that two or more grades were proper to determine the grading in the middle slaughter house, however only two grades were proper for the others. Freshness, bruise, wound, trimming and the inclusion of foreign materials affected to the grading. Poultry meat cuts were accepted for the grading in large-slaughter house, but whole poultry carcass was accepted for the grading in the others. Most managers agreed with that the grading system was only applied for the amounts requested. They also suggested that the grading system of poultry meat cuts were necessary in near future, even though it was not urgent to apply.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.329-336
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• 2009

This experiment was carried out to know adaptability and forage production and quality of corn hybrid for silage at paddy field from 2007 to 2008 at Chungnam province. Growth, forage production and quality of silage corn showed more well drained condition than poorly drained condition at paddy field. Among growth characteristics, 'Kwangpyongok' and 'DK697' hybrids were somewhat strong for waterlogging, then and good at stay green, lodging, disease and insect resistance. Fresh yield of 'DK697' hybrid at poorly drained paddy field was the highest as 32,610 kg per ha among corn hybrids. The dry yield of 'P32P75' hybrid at poorly drained paddy field was the highest as 14,910 kg per ha The result of this study showed that 'P32P75', 'DK697', 'Kangdaok' and 'Kwangpyongok' hybrids had good growth characters and forage productivity at poorly drained paddy field and dry matter yield at poorly drained paddy field was 65.6% level compared with well drained paddy field.

• Journal of Sericultural and Entomological Science
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• v.49 no.2
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• pp.37-42
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• 2007

Lactoferrin, an ion-binding 80-kDa glycoprotein, has been suggested to have many biologic activities, such as facilitating ion absorption and having antimicrobial and anti-inflammatory effects. Several of these activities are likely to only be facilitated by human lactoferrin because they depend on the binding of human lactoferrin to specific receptor. To produce recombinant human lactoferrin to animal foods using transgenic silkworm, Bombyx mori L, we have cloned and sequenced the cDNA encoding for a human lactoferrin (HLf) from the mRNA in mammary tumor line (GI-101). As a result, the 2.5-kb fragment of HLf gene was cloned with pGEM-T vector and then this fragment was sequenced. In the nucleotide sequence analysis, single open reading frame of the 2,136-bp encoding for a polypeptide of 712 amino acid residues was detected. On the other hand, we constructed a recombinant plasmid(pPT-HLf), containing human lactoferrin gene for germ line transformation of the silkworm using a piggyBac transposon-derived vector. A nonautonomous helper plasmid encodes the piggyBac transposase. Approximately 6.7% of individuals in the G0 silkworms expressed green fluorescent protein (GFP). PCR analyses of GFP-positive silkworms (G0 and G1) revealed that independent insertions occurred frequently. Furthermore, Western blot analysis showed that the recombinant HLf expressed in hemolymph has the same molecular weight (80 kDa) as a native protein. On the basis of these experiments, expression of HLf in next generation of transgenic silkworm is now in process.

• Journal of the Economic Geographical Society of Korea
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• v.14 no.4
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• pp.553-567
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• 2011

Since their establishment in 1999, Regional Development Agencies in England have been a critical catalyst for sustaining regional development, by linking the central government and the region. Nevertheless, the new Coalition Government formed in May 2010 is to abolish RDAs and is to be replaced by Local Enterprise Partnerships(LEP). This article looks at the performance and the present reform process of the English Regional Development Agencies(RDA). It argues that RDAs have been a sort of laboratory to expose the possibility of realizing the new regionalist approach away from the government-centered approach. Nevertheless, the case of RDAs in England shows that the RDA model is less likely to be effective without substantial devolution. It shows that the extent to which RDAs contribute to the regional economy tends to be much higher in soft infra-centered projects, such as business supports and skills development, than physical infrastructure projects. It argues that the LEP model, which is to replace RDAs, is controversial, especially in terms of spatial boundary and financial structure.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.3
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• pp.211-216
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• 2011

The present study was conducted to determine the optimum in vitro conditions for callus induction and plant regeneration from mature seed derived callus of four cultivars of Timothy. In order to investigate the effects of genetic variations of timothy in tissue culture, calli were induced from mature seeds of four varieties, 'Colt', 'Chair', 'Richmond' and 'Hokuo' and plant regeneration frequency was compared. Significant differences were observed among the cultivars in both callus induction and plant regeneration. Genotype 'Colt' consistently performed best in the callus subculture and plant regeneration. The complete plantlets were thereafter transplanted to grow under greenhouse condition. Regenerated timothy plants were morphologically uniform with normal leaf and growth pattern.

• Korean Journal of Plant Resources
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• v.32 no.1
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• pp.45-52
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• 2019

In the process of adapting climate change, the government needs to provide policy and technical support necessary for growing promising crops imported from abroad. Therefore, this study was conducted to survey and analyze the conditions of growth of imported foreign crops and to derive response tasks. As a result, tropical and subtropical vegetables were cultivated 18 crops in 920 farms in 321 ha area (in 2015 year). The cultivation scale decreased in the order of Curcuma aromatica, Momordica charantia, Asparagus aethiopicus, Allium hookeri and Herbs. Tropical and subtropical fruits were cultivated 9 crops in 264 farms in 106.5 ha area (in 2015 year). Special and medicinal crops introduced abroad cultivated 10 crops in 753 farms in 276.3 ha area (in 2015 year). The cultivation scale decreased in the order of Curcuma longa, Glycyrrhiza uralensis, Lepidium meyenii and Moringa oleifera. For the stable settlement of domestic growth of tropical and subtropical crops introduced abroad, there should be safety and economic feasibility in terms of the cultivation environment according to the domestic culture adaptation test. Consideration needs to be given to the use of locally grown products in Korea, the securing of distribution and sales markets, and the competitiveness of imported products.

• Journal of Applied Biological Chemistry
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• v.52 no.1
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• pp.1-7
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• 2009

For the development of qualitative PCR detection method of genetically modified (CM) rice, rice species-specific gene, OsCc-1 (rice cytochrome c gene), was selected as suitable far use as an endogenous gene in rice. The primer pair OsCytC-5'/3'with 111 bp amplicon was used for PCR amplification of the rice endogenous gene, OsCc-1 and no amplified product was observed from 8 different crops as templates. Qualitative PCR method was carried out with stack traits of L528$\times$CryIAc1 GM rice developed in Korea. For the qualitative PCRs, some primer pairs were designed with a construct-specific and event-specific type based on T-DNA and junction sequences of T-DNA in GM rice. Actck-5'/3' amplifying between actin promoter and OsCK1 gene introduced in LS28 gave rise to an amplicon 306 bp; also, CrLB-5'/3' from CryIAcl and CKRB-5'/3'amplifying the junction region of T-DNA and genome sequence from LS28 as event-specific primers gave rise to an amplicon 142 bp and 91 bp, respectively. These primer pairs for the detection of event-specific targets not produced PCR amplicons on non-CM rice and various crops in contrast to event lines. Therefore, in this study we verified that event-specific primers were effective to specifically detect stack trait lines and demonstrated that this method presented could be provided with the detection-method data for risk assessment analysis of GM rice to be commercialized.