• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.453-457
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• 1994

Six local strains collected in Korea and one cultivar (Samdo) introduced from Japan were applied for comparative cultivation to investigate growth characteristics, root yield potentials and saikosaponin contents of root in Bupleurum falcatum L. The one and two year old plants of the native local strains flowered in August 2~3 and July 16~18, respectively, whereas Samdo was late flowering type, being delayed by 18~32days and sustained the growth of above ground parts to the end of November. All the native local strains have similiar characteristics but Samdo was completely different in plant and root type. Local strain collected from Chunchon showed better in growth and root characteristics, consequently resulting high yield of dry roots and saikosaponin contents in both one and two year old plants. The average yield of two year cultivation system was 2.6 times high as 105Kg /10a, compared with that of one year.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.231-235
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• 2010

'Jopung' (Avena sativa L.), a winter oat for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2009. It was derived from an original cross between the $F_1$ hybrid of 'Early80' and 'PA202-210-S' and 'Walken' in 1999. Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Suwon and Yeoncheon, respectively. A line, 'SO99006-B-YB-29-Y1', was selected for earliness and good agronomic characteristics and designated as a line name of 'Gwiri65'. The line 'Gwiri65' was subsequently evaluated for earliness and forage yield in four different locations, Yesan, Iksan, Kimjae, and Jeju, from 2007 to 2009 and finally named as 'Jopung'. Over 3 years, the average forage dry matter yield of cultivar 'Jopung' harvested at milk-ripe stage was $17.3MT\;ha^{-1}$, compared with $13.6MT\;ha^{-1}$ of check cultivar 'Swan'. The silage quality of 'Jopung' was superior to the check cultivar 'Swan' in crude protein content, and was similar to the check cultivar in percent total digestible nutrients (TDN). Cultivar 'Jopung' is suitable primarily for winter planting in the southern regions of Korean peninsula.

• Journal of Korean Library and Information Science Society
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• v.44 no.3
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• pp.379-403
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• 2013

This study aims to 1) analyse the current RDA application and training and 2) develop an on-the-job training model for Korean library and information professionals. For this purpose, this study collected and analysed comprehensively RDA on-the-job training programs in the major foreign national libraries, consortium, college libraries, etc. Then, this study presented application methodology of the devised training model to the National library of Korea depending on the RDA application process. Also, the significant consideration for the RDA implementation by type of libraries was suggested.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.1
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• pp.9-14
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• 2012

This experiment was carried out to know adaptability, forage production and quality of corn hybrid for silage at three regions during 2007 to 2008. Among agronomic characteristics, 'CAP444NG' hybrid was somewhat strong for good stay green and higher stem height as 294cm. The fresh and dry matter yield of 'CAP444NG' hybrid were the highest 61,111 kg/ha and 20,635 kg/ha, respectively. The crude protein of 'CAP444NG' hybrid was somewhat higher than other hybrids as 7.9%. The result of this study indicated that 'CAP444NG' hybrid could be recommended as having good characters according to forage production and stay green and for silage in Korea.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.764-771
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• 2013

Recently, Cucumber mosaic virus (CMV)-P1 infection in pepper cultivation is very serious problem, which causes low marketability and yield. In this research, 56 domestic pepper cultivars including 20 PR (Phytophthora resistance) cultivars, 31 foreign pepper lines collected from USA and 112 genetic pepper resources form RDA Genebank were used for CMV-P1 resistance evaluation. Resistance evaluation was performed at 24 days and 51 days after artificial inoculation of peppers by enzyme-linked immunosorbent assay (ELISA). Among the 36 domestic cultivars 'Muhanjilju' were resistant to CMV-P1. All 20 PR cultivars and 29 foreign peppers except 'NuMex Twilight' and 'Chainese Giant' were susceptible. Among 112 pepper lines from RDA Genebank, nine pepper lines were resistant, and 17 pepper lines were moderately resistant, and 86 pepper lines were susceptible. Almost all domestic peppers on the market were highly susceptible to CMV-P1, whereas 17.2% of foreign pepper cultivars and genetic pepper lines from RDA Genebank were resistant or moderately resistant. Resistant pepper lines selected in this study can be used as genetic sources for breeding CMV-P1 resistant pepper.

• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.7-11
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• 2004

This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, $F_1$ plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 $A_2$ lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(Ba $r^{R}$)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of $F_1$ plants crossed between transformant and cultivar or transform ant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety.y.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.261-270
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• 2009

Oxidative stress is a major damaging factor for plants exposed to environmental stresses. In order to develop transgenic rice plants with enhanced tolerance to various environmental stresses, PsAPX1, the gene of ascorbate peroxidase isolated from Pisum sativum was expressed in chloroplast under the control of an oxidative stress inducible sweet potato peroxidase2 (SWPA2) promoter (referred to as PsAPX1 plants). PsAPX1 transgenic plants showed enhanced tolerance to various environmental stresses, such as 170 mM NaCl, UV-B, ozone, 20% PEG, and drought in compared with non-transgenic (NT) plants. These results suggest that chloroplast-targeted over-expression of PsAPX1 gene could be very useful strategy for developing transgenic rice plants with increased tolerance to environmental stresses.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.123-130
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• 2013

This study was conducted to investigate the effects of climate change on the growth characteristics and dry matter yields of silage corn hybrids in fields of forage crops of Hanwoo Experiment Station, National Institute of Animal Science, RDA, from Apr. 2009 to Sep. 2011. Corn hybrids were cultivated in Daegwallyeong of Gangwon Province, at an altitude of 760 m. Corn varieties used in this study consisted of 5 domestic varieties and 5 foreign varieties. Differences of silk days according to years occurred at an average of 5.5 days. The silk periods of domestic varieties occurred from Aug. 8 to 12, while that of overseas varieties was from Aug. 5 to 11. Silk days of domestic varieties occurred approximately 3 to 4 days earlier than those of oversea varieties. Silk days of Kwangpyeongok and P3156 belonging to the early varieties were Aug. 8 and 5, respectively. Kwangpyeongok and P3156 were Aug. 8 and 5, respectively. Pyeonganok and DK729 belonged to late varieties. The mean plant height of corn was approximately 231 cm, while those of Kwangpyeongok and Pyeonganok were 236 cm and 237 cm, respectively. The mean stem diameter and ear height of corn were approximately 23.2 mm and 94 cm, respectively. In the case of stem diameters, those of Cheonganok and 33J56 were 86 cm and 80 cm, respectively, while Gangdaok grew to a greater height (enter height) than other varieties. Dry matter yields of Kwangpyeongok and Pyeonganok were higher than those of other varieties. The mean leaf ratio of corn was 39.3%, while that of domestic varieties increased as compared to foreign varieties. The average DM yield of corn was 16,653 kg/ha, while those of 32P75, P3156, Pyeonganok, P3394 and Kwangpyeongok were 18,901, 17,997, 17,675, 17,194, 17,188 kg/ha, respectively. Total digestible nutrient (TDN) yields of 32P75, P3156, P3394, Pyeonganok and Kwangpyeongok were 13,381, 12,590, 12,532, 12,140 and 12,036 kg/ha, respectively. Corn crude protein (CP), in vitro dry matter digestibility (IVDMD), neutral detergent fiber (NDF), acid detergent fiber (ADF) and TDN were 7.8%, 74.2%, 42.4%, 23.5% and 70.3%, respectively. In the case of nutritive values of corn, there was no significant difference between of corn varieties of domestic and foreign origin.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.249-256
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• 2007

This experiment was conducted to compare the agronomic characteristics and productivity in introduced hairy vetch cultivars and developed new Korean Hairy vetch cultivars in two areas of the experimental field of Grassland and Forage Crops Division, National Institute of Animal Science from 2005 to 2006. The experiment was arranged in randomized complete block design with three replications. A total of 11 hairy vetch cultivars used in this study including 9 introduced cultivars (Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712, Minnie, Barlosa and Capello) and 2 Korean cultivars (Cold green and Cheong pa). The early flowering varieties of Minnie, Barlosa, Capello and Cold green were grown as early maturity cultivars, while the late flowering varieties of Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712 and Cheong pa were grown as medium and late maturity. The winter hardiness of hairy vetch was good except for Minnie, Barlosa and Capello of early maturity cultivars. In this study, dry matter yield of VV4712 was the highest of 11 hairy vetch cultivars. And dry matter yield of medium and late maturity vetch increased in the middle region while that of early maturity increased in the south. The new Korean cultivar, Cheong pa, as a medium and late maturity hairy vetch was not bad compared to introduced cultivars and Cold green as an early maturity hairy vetch was an excellent cultivar compared to introduced cultivars in both regions. Latigo in NDF (neutral detergent fiber) and Welta in ADF (acid detergent fiber) were lower than other cultivars, while Sander in IVDMD (in vitro dry matter digestibility) and CP (crude protein) content were higher than other cultivars. The results of this experiment indicated that the productivity of hairy vetch cultivars was highest in VV4712, and Cheong pa and Cold green of new Korean cultivar were the promising cultivars of hairy vetch in Korea.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.210-216
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• 2013

Efficient regeneration methods and transformation system are a priority for successful application of genetic engineering to vegetative propagated plants such as grape (Vitis labrusca L.). This research is to establish shoot regeneration system from plant explants for 'Campbell Early', 'Tamnara', 'Heukgoosul', 'Heukbosek' using two types of plant growth regulators supplemented to medium. The highest adventitious shoot regeneration rate of 5% was achieved on a medium containing of Murashige and Skoog (MS) inorganic salts and Linsmaier and Skoog (LS) vitamins, 2 mg/L of TDZ and 0.1 mg/L of IBA. Leaf tissue of 'Campbell Early', was co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, gus gene as reporter gene and resistance to kanamycin as selective agent, the other Agrobacterium strains, GV3101 containing the vector pB7 WG2D carrying with mPAP1-D gene. mPAP1-D is a regulatory genes of the anthocyanin biosynthetic pathway. 'Campbell Early' harboring mPAP1-D gene was readily able to be selected by red color due to anthocyanin accumulation in the transformed shoot. These results might be helpful for further studies to enhance the transformation efficiency in grape.