• Title/Summary/Keyword: RCL

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Evaluation of Antioxidant and Antimicrobial Activities of Ethanol Extracts of Three Kinds of Strawberries

  • Seleshe, Semeneh;Lee, Jong Seok;Lee, Sarah;Lee, Hye Jin;Kim, Ga Ryun;Yeo, Joohong;Kim, Jong Yea;Kang, Suk Nam
    • Preventive Nutrition and Food Science
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    • v.22 no.3
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    • pp.203-210
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    • 2017
  • The antioxidant and antimicrobial activities of three kinds of strawberry ethanol extracts from Robus corchorifolius L. f. (RCL), Rubus parvifolius L. var. parvifolius (RPL), and Duchesnea chrysantha Miq. (DCM) were investigated. The RPL was highest (P<0.05) in phenolic, flavonoid, and anthocyanin contents. 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities of RPL and DCM extracts were higher than that of RCL (P<0.05). Hydrogen peroxide scavenging activity of RPL was high compared to DCM and RCL (P<0.05). RCL exhibited a significant (P<0.05) potent antioxidant activity in nitric oxide radical inhibition. Inhibition diameter zone (nearest mm) of extracts against the test bacteria ranged from 11.5 in RCL to 12.5 in DCM against Staphylococcus aureus, from 10.5 in RCL to 13.5 in DCM against Streptococcus pneumoniae, from 8.5 in DCM to 10.5 in RCL against Escherichia coli, and the same inhibition of 10 mm in three of the extracts against Klebsiella pneumoniae. However, there was no inhibition against fungi Aspergillus niger and Candida albicans. Three of the extracts had the same minimum inhibitory concentration values of 12.50, 12.50, and $6.25{\mu}g/mL$ against S. aureus, K. pneumoniae, and S. pneumoniae, respectively. On the other hand, MIC values of 12.50, 12.50, and $6.50{\mu}g/mL$ were recorded for RPL, DCM, and RCL against E. coli, respectively. The result of present study revealed that extracts from three kinds of strawberries could be potential candidates as antioxidant and antimicrobial sources for functional food industries.

Effects of Root of Curcumin longa on LPS-induced Lung Injury (강황이 LPS로 유도된 폐손상에 미치는 영향)

  • Oh, Ji-Seok;Yang, Su-Young;Kim, Min-Hee;Namgung, Uk;Park, Yang-Chun
    • The Journal of Korean Medicine
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    • v.34 no.1
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    • pp.89-102
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    • 2013
  • Objectives: This study aimed to evaluate the effects of root of Curcumin longa (RCL) on LPS-induced COPD (chronic obstructive pulmonary disease) model. Materials and Methods: Extract of RCL was treated to RAW 264.7 cells and LPS-induced COPD mouse model. Then, various parameters such as cell-based protective activity, airflow limitation, accumulation of immune cells and histopathological finding were analyzed. Results: RCL showed a protective effect on LPS-induced cytotoxicity in RAW 264.7 cells. RCL treatment also revealed a protective effect on LPS-induced lung injury in a COPD mouse model. This effect was demonstrated via the reduction of accumulation of immune cells and pathophysiological regulation of caspase 3, elastin and collagen in lung tissue. Conclusions: These data suggest that RCL has a pharmaceutical property on lung injury. This study provides scientific evidence for the efficacy of RCL for clinical application to COPD patients.

Rubus croceacanthus Leveille inhibits mast cell-mediated immediate-type allergic reactions and tumor necrosis factor-$\alpha$ secretion

  • Moon, Phil-Dong;Park, In-Young;Na, Ho-Jeong;Hong, Seung-Heon;Kim, Hyung-Min
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2003.11a
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    • pp.99-99
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    • 2003
  • We studied the effect of Rubus croceacanthus Leveille (RCL) on mast cell-mediated anaphylactic reactions. RCL inhibited compound 48/80-induced systemic anaphylactic shock. When RCL was given at concentrations ranging from 0.01 to 1 mg/$m\ell$, the histamine release from rat peritoneal mast cells induced by compound 48/80 was reduced in a dose-dependent manner. RCL also inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl IgE. In addition, RCL inhibited phorbol 12-myristate 13-acetate and A23187-induced tumor necrosis factor-${\alpha}$ secretion from human mast cell line HMC-1 cells. These results indicate that RCL may possess a strong anti-anaphylactic activity.

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Comparison of Analysis Methods for Detection of Replication Competent Virus and Functional Titers of HIV-l Based Lentivirus Vector (HIV-l 유래 렌티바이러스 벡터의 복제가능 바이러스 검출과 역가측정 분석방법 비교)

  • Chang Seok Kee;Oh Il Ung;Jeong Jayoung;Ahn Kwang Soo;Sohn Yeowon
    • YAKHAK HOEJI
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    • v.49 no.3
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    • pp.217-224
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    • 2005
  • Human Immunodeficiency Virus type 1 (HIV-l) based lentivirus vector has demonstrated great potential as gene therapy vectors mediating efficient gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be confirmed that vector preparations are safe and not contaminated by replication competent lentivirus (RCL) related to the parental pathogenic virus, HIV-l. In this study, we would like to establish the method for titration and RCL detection of lentivirus vector. The titration was determined by vector expression containing the green fluorescent protein, GFP in transduced cells. The titer was $1{\times}10^7$ Transducing Unit/ml in the GFP expression assay and $8.9{\times}10^7$ molecules/ml in the real-time PCR. Also, for the detection of RCL, we have used a combination method of PCR and p24 antigen detection. First, PBS/psi and VSV-G region in the genomic DNA of transduced cells was detected by PCR assay. Second, transfer and expression of the HIV-1 gag gene was detected by p24 ELISA. In an attempt to amplify any RCL, the transduced cells were cultured for 3 weeks (amplification phase) and the supernatant of amplified transduced cell was used for the second transduction to determine whether a true RCL was present (indicator phase). Analysis of cells and supernatant at day 6 in indicator phase were negative for PBS/psi, VSV-G, and p24 antigen. These results suggest that they are not mobilized and therefore there are no RCL in amplification phase. Thus, real-time PCR is a reliable and sensitive method for titration and RCL detection of lentivirus vector.

Optimal energy saving model for RCL(Re-Coiling Ling) hydraulic system (ICCAS 2003)

  • Yi, Seoung-Ung;Won, Sang-Chul
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.1736-1740
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    • 2003
  • POSCO Kwang-Yang Works #2 Cold Rolling Mill RCL has the main hydraulic system that controlled on/off control method. In the aspect of the consumption of electrical energy this system has some problem. So, we present a continuous control method instead of on/off control method that controls the pressure of main hydraulic system with saving the electric energy.

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Comparison Study of Experimental Neutron Room Scattering Corrections with Theoretical Corrections in RCL's Calibration Facility at KAERI (한국원자력연구소 중성자교정실에 대한 중성자산란보정인자 결정연구)

  • Yoon, Suk-Chul;Chang, Si-Young;Kim, Jong-Soo;Kim, Jang-Lyul;Kim, Bong-Hwan
    • Journal of Radiation Protection and Research
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    • v.22 no.1
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    • pp.29-33
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    • 1997
  • Neutron room scattering corrections that should be made when neutron detectors are calibrated with a $D_2O$ moderated $^{252}Cf$ neutron source in the center of a calibration room are considered. Such room scattering corrections are dependent on specific neutron source type, detector type, calibration distance, and calibration room configuration. Room scattering corrections for the responses of a thermoluminescence dosimeter and two different types of spherical detectors to neutron source in the Radiation Calibration Laboratory(RCL) neutron calibration facility at the Korea Atomic Energy Research Institute(KAERI) were experimentally determined and are presented. The measured room scattering results are then compared with theoretical results calculated by predicting room scattering effects in terms of parameters related to the specific configuration. Agreement between measured and calculated scattering correction is generally about 10% for three kinds of detectors in the calibration facility.

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Separation of Rare Earth Elements Using Ln-EDTA Eluent in an Anion Exchanger (음이온 교환수지에서의 희토류-EDTA 용리액에 의한 희토류 원소의 분리)

  • Cha, Ki-Won;Park, Chan-Il;Chang, Byung-Doo;Hong, Sung-Wook
    • Analytical Science and Technology
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    • v.10 no.1
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    • pp.53-59
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    • 1997
  • Chloride form anion exchange resin was used to separate one of the elements from the rare earth mixture using respectable Ln-EDTA eluent. $Sm^{3+}$, $La^{3+}$ or $Ce^{4+}$ complexed with EDTA was passed through the resin column and eluted with a Sm-EDTA solution as an eluent. Here all the rare earth element ions except $Sm^{3+}$ were passed. Adsorbed $Sm^{3+}$ in resin was eluted with 1.0 M HCl solution. If La-EDTA solution as an eluent was used to separate lanthanum ions, lanthanum ions were eluted together with other rare earth elements. When Ce-EDTA solution was also used for separation of $Ce^{4+}$, it was eluted in the region of other rare earth elements. In the case of Sm-EDTA elution, the elution mechanism was as follows : Absorption : $RCl+Ln-Y^-{\leftrightarrows}RLnY+Cl^-$, Sm-EDTA elution : $RLnY+Sm-Y^-{\leftrightarrows}RSmY+Ln-Y^-$, HCl elution : $RSmY+HCl{\leftrightarrows}RCl+Sm-Y^-$.

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Performance improvement of repetitive learning controller using AMN (AMN을 이용한 반복학습 제어기의 성능개선)

  • 정재욱;국태용;이택종
    • 제어로봇시스템학회:학술대회논문집
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    • 1997.10a
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    • pp.1573-1576
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    • 1997
  • In this paper we present an associative menory network(AMN) controller for learning of robot trajectories. We use AMN controller in order to improve the performance of conventional learning control, e.g. RCL, which had studied by Sadegh et al. Computer simulations show the feasibility and effectiveness of the proposed AMN controller.

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A Localization Algorithm for Underwater Wireless Sensor Networks Based on Ranging Correction and Inertial Coordination

  • Guo, Ying;Kang, Xiaoyue;Han, Qinghe;Wang, Jingjing
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.13 no.10
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    • pp.4971-4987
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    • 2019
  • Node localization is the basic task of underwater wireless sensor networks (UWSNs). Most of the existing underwater localization methods rely on ranging accuracy. Due to the special environment conditions in the ocean, beacon nodes are difficult to deploy accurately. The narrow bandwidth and high delay of the underwater acoustic communication channel lead to large errors. In order to reduce the ranging error and improve the positioning accuracy, we propose a localization algorithm based on ranging correction and inertial coordination. The algorithm can be divided into two parts, Range Correction based Localization algorithm (RCL) and Inertial Coordination based Localization algorithm (ICL). RCL uses the geometric relationship between the node positions to correct the ranging error and obtain the exact node position. However, when the unknown node deviates from the deployment area with the movement of the water flow, it cannot communicate with enough beacon nodes in a certain period of time. In this case, the node uses ICL algorithm to combine position data with motion information of neighbor nodes to update its position. The simulation results show that the proposed algorithm greatly improves the positioning accuracy of unknown nodes compared with the existing localization methods.

Dynamic analysis of charged human body electrostatic discharge - comparison with quasi-static discharge (대전인체의 정전기 방전에 대한 동적해석 - 준정적해석과의 비교)

  • 이종호;김두현;김상철;김상렬
    • Proceedings of the Korean Institute of Industrial Safety Conference
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    • 1998.05a
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    • pp.257-262
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    • 1998
  • In order to understand the characteristics of electrostatic discharge (ESD) by a charged human body, a dynamic analysis method based on RCL circuit and a quasi-static analysis method based on two-body model are introduced. In this paper, these methods calculate waveforms, discharge energy and potential difference to analyze the ESD phenomena from given initial conditions and geometry. Results are compared and discussed.

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