• 통합자연과학논문집
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• 제9권3호
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• pp.171-176
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• 2016

Rap1 is a key regulator of cell adhesion and migration. Although increasing evidence indicates that the Rap1 signaling pathway is involved in the process of bone remodeling, the mechanism by which Rap1 regulates osteoblastic differentiation and cell adhesion remains unknown. Here, we investigated the morphological characteristics and osteoblastic differentiation of cells expressing constitutively activated form of Rap1A (Rap1ACA) or Rap1 GTPase activating protein Rap1GAP and found that activated Rap1 induces osteoblastic differentiation and cell adhesion as well as cell spreading. When osteoblastic differentiation was induced, Rap1ACA cells showed considerably higher levels of calcium deposits than the wild-type and Rap1GAP-overexpressing cells did. Rap1ACA cells showed increased spreading and size, as well as strong cell adhesion and significantly decreased growth rates. F-actin staining using phalloidin revealed several thin thread-like filopodia around the protrusions in Rap1ACA cells, which possibly contribute to the increased cell adhesion.

• 한국지반공학회논문집
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• 제20권5호
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• pp.127-134
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• 2004

본 연구는 RAP공법의 구조물 기초로서의 안정성 및 사용성 확보를 위한 기초연구로써, RAP공법이 적용된 지반의 지지력 및 파괴거동을 실내모형토조시험을 통하여 검토한 것이다. 본 연구에서는 RAP 공법을 구조물 기초로서의 파괴거동을 파악하기 위한 실내모형실험을 수행하였다. 사질토 지반에서 상대밀도별(60%, 70%, 90%), 직경 별(45mm, 60mm, 70mm)로 RAP를 설치한 후 깊이별(5cm, 10cm, 15cm, 20cm, 25cm, 30cm)로 토압계를 각각 RAP에 인접한 곳과 RAP 중심에서 1.0D 떨어진 곳에 설치하여 하중재하 실험을 수행하여 RAP의 지지력 및 파괴거동을 검토하였다. 실험 결과 RAP의 파괴거동은 깊이 5∼10cm(1.0D∼2.0D)에서 최대 횡토압을 받아 일반적인 RAP의 파괴 형태인 Bulging 파괴가 발생하는 것으로 판단되었다. 그리고 RAP에 하중을 가하였을 경우 상대밀도가 낮을 경우에는 RAP의 직경변화가 컸으며 상대밀도가 높을 경우에는 RAP의 직경변화가 작음을 알 수 있었다. 깊이별 횡방향 응력 분포도는 상부에서 횡토압의 영향이 크게 나타났으며 하부로 내려갈수록 작아지는 것을 알 수 있었다.

• Structural Engineering and Mechanics
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• 제67권3호
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• pp.283-290
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• 2018

This paper presents the results of a study that investigated the influence of using recycled coarse aggregate (RCA) and recycled asphalt pavement (RAP) on the properties of pervious concrete (PC). The natural aggregate (NA) was replaced by RCA and RAP in the PC with replacement levels of 0%, 20%, 40%, 60% and 80% by the total weight of NA, respectively. In addition to incorporating RAP and RCA in the same mixes with replacement levels of: (1) 20% RAP and 80% RCA; (2) 60% RAP and 40% RCA; and (3) 80% RAP and 20% RCA. Water permeability, thermal conductivity, density, porosity, void content, and compressive, splitting tensile and flexural strengths were studied in this paper. The results showed that using RCA, RAP, and (RAP-RCA) enhanced the properties of PC in general and improved the mechanical properties significantly in particular. The optimum mix was reported to be the 60% RAP and 40% RCA. Accordingly, the RAP has the potential to be used in PC in order to reduce the negative impact of RAP on the human health and environment.

• Structural Engineering and Mechanics
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• 제62권3호
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• pp.357-364
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• 2017

This paper presents the results of a study that investigated the improvement of the mechanical properties of coarse and fine recycled asphalt pavement (RAP) produced by adding silica fume (SF) with contents of 5%, 10%, and 15% by total weight of the cement. The coarse and fine natural aggregate (NA) were replaced by RAP with replacement ratio of 20%, 40% and 60% by the total weight of NA. In addition, SF was added to NA concrete mixes as a control for comparison. Twenty eight mixes were produced and tested for compressive, splitting tensile and flexural strength at the age of 28 days. The results show that the mechanical properties decrease with as the content of RAP increases. And the decrease in the compressive strength was more in the fine RAP mixes compared to the coarse RAP mixes, while the decrease in the splitting tensile and flexural strength was almost the same in both mixes. Furthermore, using SF enhances the mechanical properties of RAP mixes where the optimum content of SF was found to be 10%, and the mechanical properties enhancement of coarse RAP were better than fine RAP mixes. Accordingly, the RAP has the potential to be used in the concrete pavements or in other low strength construction applications in order to reduce the negative impact of RAP on the environment and human health.

• Advances in concrete construction
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• 제10권6호
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• pp.489-498
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• 2020

This paper investigates mechanical properties of roller compacted concrete (RCC) involving reclaimed asphalt pavement (RAP). In this way, a set of 276 cylindrical RCC specimens were prepared with different RAP sizes (i.e., fine, coarse & total) at various ratios (i.e., 10%, 20%, and 40%). Results reveal that incorporation of RAP decreases unconfined compressive strength (UCS), modulus of elasticity (E), and indirect tensile (IDT) strength of RCC. For each RAP size, a regression model was used to maximize RAP content while satisfying the UCS lower limit (27.6 Mpa) mentioned by ACI as a minimum requirement for RCC used in pavement construction. Moreover, UCS of RAP incorporated mixes, dissimilar to that of control mixes, was found to be sensitive and insensitive to the testing temperature and curing time after 7 days, respectively. The results also demonstrate that the higher amounts of RAP, the more flexibility in RCC is. This issue was also proved by the results of modulus of elasticity test. In addition, the toughness index (TI) shows that increase in RAP content leads to up to 43% increase in energy absorbance capacity of RCC.

• Parasites, Hosts and Diseases
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• 제60권6호
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• pp.393-400
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• 2022

Human infection with simian malaria Plasmodium knowlesi is a cause for concern in Southeast Asian countries, especially in Malaysia. A previous study on Peninsular Malaysia P. knowlesi rhoptry associated protein-1 (PkRAP1) gene has discovered the existence of dimorphism. In this study, genetic analysis of PkRAP1 in a larger number of P. knowlesi samples from Malaysian Borneo was conducted. The PkRAP1 of these P. knowlesi isolates was PCR-amplified and sequenced. The newly obtained PkRAP1 gene sequences (n=34) were combined with those from the previous study (n=26) and analysed for polymorphism and natural selection. Sequence analysis revealed a higher genetic diversity of PkRAP1 compared to the previous study. Exon II of the gene had higher diversity (π=0.0172) than exon I (π=0.0128). The diversity of the total coding region (π=0.0167) was much higher than those of RAP1 orthologues such as PfRAP-1 (π=0.0041) and PvRAP1 (π=0.00088). Z-test results indicated that the gene was under purifying selection. Phylogenetic tree and haplotype network showed distinct clustering of Peninsular Malaysia and Malaysian Borneo PkRAP1 haplotypes. This geographical-based clustering of PkRAP1 haplotypes provides further evidence of the dimorphism of the gene and possible existence of 2 distinct P. knowlesi lineages in Malaysia.

• 한국구조물진단유지관리공학회 논문집
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• 제23권2호
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• pp.116-121
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• 2019

본 연구에서는 분쇄한 순환골재 분말(RAP)을 건설소재로 활용하기 위하여 순환골재 및 RAP의 재료적 특성을 파악하고, 시멘트 대체재로 RAP를 적용한 모르타르의 물리 역학적 특성을 분석하였다. RAP 입도분석결과, 볼밀 시간이 증가함에 따라 0.6mm 입도의 분포량이 증가하고, 조립률은 감소하는 것으로 나타났다. RAP를 치환한 모르타르의 유동성은 Plain 보다 향상되었으며, 이는 RAP를 결합재 대체재로 적용함으로서 잉여수의 증가하고 이로 인해 유동성이 증가된 것으로 판단된다. RAP를 적용한 모르타르의 압축강도 평가 결과, 치환율이 증가함에 따라 압축강도가 낮아지는 경향을 나타내었지만, 약 10%까지는 치환하여 사용가능한 것으로 판단된다. 이상의 연구로부터 분말화한 순환골재는 잔골재 대체재로서도 품질특성을 만족할 수 있는 것으로 나타났으며, 본 연구 범위에서는 결합재 대체재로 약 10% 적용시 유동성 개선 및 강도 확보가 가능할 것으로 판단된다.

• BMB Reports
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• 제42권11호
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• pp.764-768
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• 2009

The tandem ubiquitin-interacting motif (UIM) domain located at the N-terminus of Receptor Associated Protein 80 (RAP80) plays a crucial role in ionizing radiation (IR)-induced DNA damage response. RAP80 translocates to sites of IR-induced DNA damage through interaction of its UIM domain with ubiquitinated H2A and Lys63-linked polyubiquitin chains. The exact mechanism, however, through which RAP80 associates with Lys63-linked polyubiquitin chains is not clear. Here, we show by in vitro GST-pull down assays that modifying the linker region between the tandem ubiquitin binding domains of RAP80 changes the binding affinity for Lys63-linked polyubiquitin chains and affects translocation to sites of DNA breaks. Based on these findings, we suggest that the length of the linker region between the tandem ubiquitin binding domains of RAP80 may be a key factor in the binding of RAP80 with Lys63-linked polyubiquitin chains as well as in the translocation of RAP80 to DNA break sites.

• IMMUNE NETWORK
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• 제9권2호
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• pp.35-40
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• 2009

Although Rap GTPases of the Ras family remained enigmatic for years, extensive studies in this decade have revealed diverse functions of Rap signaling in the control of cell proliferation, differentiation, survival, adhesion, and movement. With the use of gene-engineered mice, we have uncovered essential roles of endogenous Rap signaling in normal lymphocyte development of both T- and B-lineage cells. Deregulation of Rap signaling, on the other hand, results in the development of characteristic leukemia in manners highly dependent on the contexts of cell lineages. These results highlight crucial roles of Rap signaling in the physiology and pathology of lymphocyte development.

• 대한토목학회논문집
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• 제36권6호
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• pp.1101-1107
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• 2016

폐아스팔트 콘크리트(RAP)의 발생이 증가함에 따라 이를 자원으로 재활용 할 수 있는 재생 아스팔트 콘크리트에 대한 관심이 높아지고 있다. 폐아스팔트 콘크리트를 아스팔트 콘크리트용 순환골재로 사용할 경우 품질저하로 인해 사용량을 제한하거나 재생첨가제를 사용하도록 하고 있다. 본 연구에서는 공정상의 편리성을 위해 재생첨가제가 포함된 중온 재생 아스팔트 바인더(WMRA)를 사용하여 RAP의 사용비율을 50% 까지 증가시킬 수 있는 재생 아스팔트 콘크리트의 실내 공용성능 평가를 수행하였다. 마샬안정도 실험 결과 RAP 30% 사용시에는 WMRA 바인더와 스트레이트 아스팔트 바인더 사용시 큰 차이가 없었으나 RAP 혼합비율이 50%로 늘어날 경우 WMRA 바인더 사용시 흐름값 34.7 (1/10 mm)로 기준을 만족시킬 수 있는 것으로 나타났다. 간접인장강도 실험의 터프니스 계산 결과 RAP 사용비율 50% 일 때 WMRA 바인더 사용시 스트레이트 아스팔트 바인더 사용시에 비해 55% 높은 값을 나타내어 균열저항성이 향상된 것을 알 수 있었다. 또한 반복직접인장실험을 통해 직접적인 균열저항성 비교 결과 WMRA 재생 아스팔트 콘크리트의 피로균열 저항성이 263% 증가하는 것으로 나타나 WMRA 바인더가 RAP 사용비율을 50%까지 높일 수 있어 RAP의 재활용에 효과적인 것으로 나타났다.