• 생명과학회지
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• 제17권11호
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• pp.1492-1496
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• 2007

CD40L를 발현하는 KU812세포와 CD40를 발현하는 항원 특이적 B 세포의 배양을 통해 IgE 클라스 스위치가 유도된다. 본 연구자는 체외면역법에 의해 건강인의 말초혈액을 이용하여 쌀 알레르겐 특이적 IgM 항체를 생성하는 B 세포주를 수립하였다. 본 연구에서는 KU812세포에 의한 쌀 알레르겐 특이적 IgE 항체의 유도를 시도하였다. 배양 전, 쌀 알레르겐 특이적 B 세포주, RA9G11과 PMA와 ionomycin을 6시간 처리하여 활성화된 KU812세포에서 CD40와 CD40L 발현량을 flow cytomerty를 통화여 각각 확인하였다. RA9G11세포와 활성화된 KU812세포는 높은 수준의 CD40와 CD40L를 각각 발현하였다. 쌀 알레르겐 특이적 IgE항체 합성 유도를 위해, 여러 가지 농도의 IL-4 존재하에, RA9G11세포와 활성화된 KU812세포를 12일 동안 배양하였다. IgE 정상영역의 mRNA 발현량과, 쌀 알레르겐 특이적 IgE 항체 생성 세포가 모든 조건에서 확인되었으며, 특히 50U의 L-4 농도 하에서 같은 비율의 RA9G11과 활성화된 KU812세포를 배양했을 때, IgE 합성이 가장 높았다. 따라서 활성화된 KU812세포에 의한 쌀 알레르겐 특이적 IgE 합성은 알레르기 질환의 치료 및 예방에 관한 연구에 기여할 것으로 사료된다.

• 한국수산과학회지
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• 제43권6호
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• pp.623-628
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• 2010

The distribution of fluoroquinolones was investigated in the carp (Cyprinus carpio) and eel (Anguilla japonica) after an oral dose of 20 mg/kg body weight/day for 35 days. Blood samples were collected at 5 and 10 hours and 1, 2, 3, 5, 7, 9, 13, 20, 30, and 35 days after treatment. The fluoroquinolone concentrations were determined high- performance liquid chromatography with an ultraviolet detector. The recovery rates of fluoroquinolones in the fish samples ranged from 91.4-96.6, 91.2-96.5, and 90.4-98.6% for concentrations of 0.1, 0.5, and $1.0\;{\mu}g/g$, respectively. In the blood of carp, ciprofloxacin, enrofloxacin, norfloxacin, and sarafloxacin reached maximums level of 11.03, 9.37, 9.10, and $9.81\;{\mu}g/g$ 10 hours, 1 day, 10 hours, and 10 hours after treatment, respectively. In the eel blood, these reached maximum levels of 12.65, 11.18, 11.91, and $8.74\;{\mu}g/g$ all at 10 hours. Carp sample concentrations of ciprofloxacin, enrofloxacin, norfloxacin, and sarafloxacin were not measurable 20, 30, 20, and 20 days after treatment, respectively, in all experiments (

• Journal of Microbiology and Biotechnology
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• 제24권2호
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• pp.264-269
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• 2014

For the production of ethanol from seaweed as the source material, thermal acid hydrolysis and enzymatic saccharification were carried out for monosugars production of 25.5 g/l galactose and 7.6 g/l glucose using Gelidium amansii. The fermentation was performed with Pichia stipitis KCTC 7228 or Saccharomyces cerevisiae KCCM 1129. When wild P. stipitis and S. cerevisiae were used, the ethanol productions of 11.2 g/l and 6.9 g/l were produced, respectively. The ethanol productions of 16.6 g/l and 14.6 g/l were produced using P. stipitis and S. cerevisiae acclimated to high concentration of galactose, respectively. The yields of ethanol fermentation increased to 0.5 and 0.44 from 0.34 and 0.21 using acclimated P. stipitis and S. cerevisiae, respectively. Therefore, acclimation of yeasts to a specific sugar such as galactose reduced the glucose-induced repression on the transport of galactose.

• 동력기계공학회지
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• 제9권4호
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• pp.110-117
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• 2005

Underwater wet arc welds were experimentally performed on 11mm thick KR-RA steel plate using six different types of flux coated electrodes of 4.0mm diameter, KSKR, KSKT, USBL, JPUW, UWEA and UWEB. From analysis of bead appearance, detachability of weld slag, spatter occurrence and arc stability, JPUW gives the best result, and UWEB is superior to KSKR and KSKT. By experimental result of hardness distribution on the weld bonds, UWEB weld has the narrowest bond structure which is probable condition to get the best mechanical properties of weld. UWEB and JPUW welds have more even hardness distribution across weld deposit and base metal. Upon polarization test to measure the respondency of corrosion, the electrode of UWEB shows the most excellent degree due to the low open circuit potential difference.

• 한국지역사회생활과학회지
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• 제20권2호
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• pp.181-191
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• 2009

This study was conducted to examine the distribution status, the physicochemical composition, and the physiological activity of Spergularia marina grown in the western region in Jeon-Ra-Nam-Do, looking for its potential as a local specialty goods. The amount sold of Spergularia marina to different areas from a rural market place was examined during April, 2008. The proportions of Spergularia marina are as follows; Yeongam(30.5%), Muan(24.3%), Naju(20.3%), Hampyeong(18.0%), and Haenam(7.0%), which were shown higher than those of Pimpinella brachycarpa. Total vitamin C and total chlorophyll contents in Spergularia marina were 68.61mg/100g DW and 58.11mg/g DW, respectively. Total amino acid contents were 331.94mg/100g DW and 20 kinds of amino acids were separated and classified. The contents of inorganic matters in Spergularia marina were as follows; Na(64.23mg/L), Mg(15.88mg/L) K(9.99mg/L), Ca(3.83mg/L). The total phenol and the total flavonoid contents of methanol extracts from Spergularia marina were 41.7mg/L and 18.9mg/L, respectively, with an extracting concentration of 2,000mg/L. The nitrite scavenging activity was highest (83.4%) in hot water extract. These results may be useful as basic data for using Spergularia marina as a local specialty goods.

• 한국수산과학회지
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• 제34권3호
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• pp.199-206
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• 2001

1999년 3월, 7월, 9월 및 11월에 섬진강 하구역에서 염분경사에 따른 영양염의 거동을 조사하였다. 조사 정점은 지리적 위치보다는 각 조사시마다 표층 염분을 현장에서 측정하여 결정하였다. 질산 질소와 규산 규소의 주요 공급원은 섬진강이었으며 아질산 질소와 인산 인은 섬진강 하구입구와 인접한 광양만에서 배출되는 도시하수와 공장폐수에 영향을 받고 있는 것으로 나타났다. 암모니아 질소는 난초도로부터 약 $6\~8km$ 지점에서 공급양상을 보였다. 암모니아 질소, 인산 인,규산 규소의 강한 제거 거동은 엽록소 $\alpha$ 농도가 $>8{\mu}g/L$ 이상으로 매우 높았던 11월 $5\~15psu$ 염분역에서 나타났다. 엽록소 a 최고치 지점에서 N : P 비가 높고 인산 인이 거의 제거된 것은 인산 인이 식물플랑크톤 성장의 제한 요인으로 작용하고 있다는 것을 시사해 주고 있다. 11월에 $5\~15psu$ 염분 역에서의 상대적으로 높은 Rb/Ra (Rb: Fluorescence before acidifcation, Ra: Fluorescence after acidification) 비는 식물플랑크톤이 이 지역에서 생리적으로 성장하기에 좋은 환경이었음을 의미한다.

• 한국식품조리과학회지
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• 제1권1호
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• pp.82-93
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• 1985

An investigation was conducted on Seoulites' meals outside home by age group and sex, and the resultant findings are as follows: 1. To effectively conduct this survey were taken 201 teen males, 242 teen females, 202 young males, 232 young females, 219 men and 195 women. 2. For the kinds of meals they have outside home, breakfast forms 0.7%, lunch 35.7%, supper 22.2%, and snacks 14.4%. 3. Men subjects "lunch out everyday" by 39.9%, young males by 30.7%, and young females by 29%. 4. The reason why they lunch out indicates "convenient" by 37.4%, "carrying a lunch box is inconvenient" by 21.2%, "hard to prepare the lunch box" by 12.9%. 5. Regarding the favorite food taken for lunch, almost all the men prefer Korean food by 73.9% 6. Ra Myun and noodle occupy the highest Percentage of young females by 79.3% ; Chinese food the highest percentage of women by 27.7%, but the lowest percentage come from Japanese food by 1.1%. 7. The preference of kinds of food when having a meal outside home is ascribed to "prefer"by 50~75.7%, the highest percentage of all. 9. The price they pay for lunch outside home amounts to less than 900 wons in teens and young males 1, 000~2, 000 wons in men and women. The price of a meal outside home per capita a day, when taken by family unit, averages to 3, 694 wons. For the number of meals outside home, "more than once a month" forms 41.7%. 9. On the mooted points raised when having a meal outside home, "unsanitary' forms 36.7%, "expensive in prices" 26.1%, "lack of nutritution" 10.6%. 10. Teen males, when viewed from food preference for lunch outside home, take Ra Myun, Ddokbokki, Chinese noodle, while teen females, Ddokbokki, bread, Chinese noodle: young males, Sundubu Baekban, Bibim Bab, Ra Myun, Yokge Jang; young females, Sundubu Baekban, pork cutlet, Bibin Bab: men, Solnone Tang, Daenjang Chikae, Baekban, Kalbi Jongsik Bibim Bab : women, Bulkogi Jongsik, Neang Moyn, and Chinese noodle. 11. When seen from the nutrition of meals taken outside home, the prices of noodle and boiled rice range from 600 to 1, 000 wons, while their energy ranges from 407 to 745 kcal, Protein from 14.2 to 30.3g. On the other hand, the Price of western food averages to 1, 900~2, 500 wons, while its energy ranges from 1, 061 to 1, 129 kcal, proteio from 45.9 to 72.9g. It is revealed that noodle or boiled rice is not nutritious enough to be taken as a meal.s revealed that noodle or boiled rice is not nutritious enough to be taken as a meal.

• 한국수산과학회지
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• 제42권4호
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• pp.329-334
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• 2009

The residues of fluoroquinolones in Olive flounder (Paralichthys olivaceus) and Black rockfish (Sebastes schlegeli) were investigated after oral doses of 20 mg/kg for 30 days. Blood samples were taken at 5 hours, 10 hours, 1 day, 2 days, 3 days, 5 days, 7 days, 9 days, 13 days, 20 days and 30 days after treatment. The concentrations of fluoroquinolones were detected by high performance liquid chromatography using a UV detector. The recovery rates of fluoroquinolones in fish samples ranged from 92.0-99.7%, 93.4-97.5% and 93.0-97.9% for treatment of 0.1, 0.5, and $1.0{\mu}g/g$, respectively. Ciprofloxacin, enrofloxacin, norfloxacin and sarafloxacin were detected in the blood of the olive flounder at 10 hours, 10 hours, 1 day and 10 hours after treatment with $6.43{\mu}g/g$, $6.07{\mu}g/g$, $11.83{\mu}g/g$ and $11.02{\mu}g/g$ as maximum level, respectively. Ciprofloxacin, enrofloxacin, norfloxacin and sarafloxacin were detected in the blood of black rockfish 1 day, 1 day, 1 day and 10 hours after treatment with $8.26{\mu}g/g$, $8.03{\mu}g/g$, $7.29{\mu}g/g$ and $9.38{\mu}g/g$ as maximum level, respectively. However, ciprofloxacin, enrofloxacin, norfloxacin and sarafloxacin were not detected in olive flounder samples at 20, 30, 20 and 20 days after treatment in any experiments (

• Childhood Kidney Diseases
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• 제9권2호
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• pp.175-182
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• 2005

목 적 : IL-1ra는 항염증반응을 가지고 있는 인자로서 IL-1 수용체와 결합하여, IL-l$\alpha$와 IL-1$\beta$의 결합을 경쟁적으로 억제시킴으로써, IL-1에 의해 매개되는 다양한 질환에서 중요한 내인성 조절인자로 작용한다. 이 유전자의 intron 2 부위에 86 bp 크기를 가지는 tandem repeat 에 의한 유전자 다형성이 존재하는데, 다양한 자가면역질환에서는 allele 2형의 빈도가 정상 인구군에 비해 유의하게 높다는 사실이 밝혀져 있다. 이에 저자들은 Henoch-$Sch{\ddot{o}}nlein$ Purpura 환자들을 대상으로 IL-1ra 유전자의 variable number tandem repeats(VNTR) 다형성을 검사하여 정상 대조군과 비교하였으며, 신장 침범 여부 및 중증의 경과에 $IL1RN^{*}2$가 어떤 영향을 미치는 지를 조사하였다. 방 법.: 1998년 1월부터 2002년 12월까지 부산 백병원 .소아과를 방문하여 Henoch-$IL1RN^{*}2$ purpura로 진단된 74명의 환자와 정상 대조군 43명을 대상으로 하였다. EDTA 처리된 전혈에서 상품화된 DNA 추출키트($QIAamp^{\circledR}$ DNA Blood Mini kit, Quiagen, USA)를 사용하여 DNA를 추출하였다. IL-1ra 유전자 다형성(polymorphysm)은 86 bp의 2, 3, 4, 5번의 반복횟수에 따라 각각 240 bp, 325 bp, 410 bp 또는 500 bp 크기의 밴드를 확인하여 결정하였다. 결 과 : HSP 환자군과 정상 대조군 모두에서 $IL1RN^{*}1$의 allele 빈도가 각각 $93.9\%,\;93.2\%$로 가장 높았으며, carriage rate도 각각 $98.6\%,\;97.9\%$로 가장 높았다. $IL1RN^{*}2$의 allele 빈도는 HSP 군에서 4.7$\%$로 대조군의 2.5$\%$에 비해 높았으나, 통계적으로 유의하지 않았다(P=0.794). Carriage rate도 HSP군에서 8.1$\%$로 대조군의 6.8$\%$에 비해 높았으나, 통계적으로 유의하지 않았다(P=0.915). $IL1RN^{*}2$의 allele 빈도는 신장 침범군에서 6.3$\%$로 비침범군의 2.9$\%$에 비해 높게 나타났으나 통계적으로 유의하지 않았다(P=0.356). Carriage rate는 신장 침범군에서 10.0$\%$, 비침범군에서 5.9$\%$였으며, 양군 사이에는 유의한 차이가 관찰되지 않았다(P=0.523). 24시간 채집뇨에서 측정한 총단백량이 1,000 mg 이상이었던 경우가 13명이었는데, 이들의 allele형은 $IL1RN^{*}1$이 11명이었으며, $IL1RN^{*}2와\;IL1RN^{*}4$형이 각각 1명씩 있었다. 마지막 추적관찰 시점까지 단백뇨가 지속되었던 환자는 4명이었으며 이들은 모두 $IL1RN^{*}1$형 이었다. 결 론 : HSP 환자군과 정상 대조군 모두에서 $IL1RN^{*}1$의 allele 빈도와 carriage rate가 가장 높았다. $IL1RN^{*}2$ allele 빈도와 carriage rate는 HSP 환자군에서 대조군과 비교하여 유의한 차이가 관찰되지 않았다. 또한 HSP 환자군에서도 $IL1RN^{*}2$ allele 빈도와 carriage rate는 신장 침범의 정도와도 유의한 관련성이 발견되지 않았다.

• 대한약침학회지
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• 제5권1호
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• pp.113-133
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• 2002

Objective: To investigate effects of Cervi Cornu on Adjuvant Athritis in rats, the edema inhibit rate, the anaJgesic effects, the number of WBC, RA facter, Platelet, the quantity of CRP, total protein, albumin and globuline in the blood serum were measured in the arthritis part. Results: The results obtained as fonows ; 1. After arthritis of Sprague dawley(SD) rats was induced by injecting Freund's complete adjuvant for 2 weeks, any treatment was not for Control group, acupunctured for Treat Ⅰ group. normal saJine was ora] administrated for the Treat Ⅱ group, Cervi Cornu Ex. was oral administrated for Treat Ⅲ, and Cervi Cornu Herbal-acupullcture was injected for Trea Ⅳ group during 2 weeks every other day. Selected point was on pressure pain point in both groups. And then the edema inhibit rate were checked. The edema inhibit rate was $46.03\%$ in Treat I group, $43.24\%$ Treat IV group, $37.44\%$ in Treat III. there was significance in the edema inhibit rate between Control group and Treat group, in order of Treat Ⅰ, Ⅳ, Ⅲ.(p<0.05) 2. The analgesic effects was $7.58{\pm}1.80$(${\times}10$gm) in Control group. $11.00{\pm}1.10$(${\times}10$gm) in Treat Ⅰ group. $99.92{\pm}1.28$(${\times}10$gm) in Treat Ⅲ group and $14.67{\pm}1.03$(${\times}10$gm) in Treat Ⅳ group. There was significance in the analgesic effects between Control group and Treat group(p<0.05) 3. The number of WBC was $14.72{\pm}1.48$(${\times}10^3$㎕) in control Group, $10.26{\pm}1.13$(${\times}10^3$㎕) in Treat Ⅰ group, $11.00{\pm}1.13$(${\times}103$㎕) in Treat Ⅱ Group and $9.63{\pm}1.75$(${\times}10^3$㎕) in Treat Ⅳ group. There was significance in the number of WBC between Control group and Treat group(p<0.05) 4. The content of total protein in the blood serum were $6.13{\pm}0.05$g/dl in control group, $5.73{\pm}0.14$g/dl in Treat I group, $5.88{\pm}0.13$g/dl in Treat Ⅲ group and $5.90{\pm}0.13$g/dl in Treat IV group. There was significance in The content of total protein in the blood serum between Control group and Treat group(p<0.05) 5. The contests of albumin in the blood serum were $2.32{\pm}0.12$g/dl in the Control group, $2.35{\pm}0.05$g/dl in Treat Ⅰ group, $2.35{\pm}0.05$g/dl in Treat Ⅱ group, $2.30{\pm}0.06$g/dl in Treat Ⅲ group, $2.42{\pm}0.08$g/dl in Treat IV group. There was no significance in The content of albumin in the blood serum between Control group and Treat group(p<0.05) 6. The contests of globulin in the blood semm were $3.68{\pm}0.08$g/dl in the Control group, $3.43{\pm}0.12$g/dl in Treat Ⅰ group, $3.55{\pm}0.10$g/dl in Treat IV group. There was significance in The content of globulin in the blood serum between Control group and Treat group(p<0.05) 7. The numbers of RA factor were $3.47{\pm}0.54$IU/ml in Control group and $2.38{\pm}0.50$IU/ml in Treat Ⅱ group. There was significance inThe numbers of RA factor between Control group and Treat group(p<0.05) 8. The numbers of platelet were $1126.33{\pm}1126.33{\pm}85.93{times}10^3$/㎕ in Control group, $1043.33{\pm}80.80{times}10^3$/㎕ in Treat Ⅰ group, $1116.82{\pm}77.93{times}10^3$/㎕ in Treat Ⅱ group, $1164.17{\pm}94.02{times}10^3$/㎕l in Treat Ⅲ group, $1076.67{\pm}54.84{times}10^3$/㎕ in Treat Ⅳ group. There was no significance in The numbers of platelet between Control group and Treat group(p<0.05) 9. The quantity of CRP were $0.05{\pm}0.01$mg/ml in Control group, $0.05{\pm}0.01$mg/ml in Treat Ⅰ group, $0.06{\pm}0.01$mg/ml in Treat Ⅱ group, $0.05{\pm}0.00$mg/ml in Treat Ⅲ group, $0.05{\pm}0.00$mg/ml in Treat Ⅳ group. There was no significance in The quantity of CRP between Control group and Treat group(p<0.05) Concluslon : From these results, it is shown Cervi Comu Herbal-acupuncture more efffective thaJJ Cervi Cornu Ex. on Adjuvant Arthritis in rats.