• Molecules and Cells
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• v.42 no.12
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• pp.850-857
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• 2019

The Gram-negative opportunistic pathogen, Pseudomonas aeruginosa, has multiple multidrug efflux pumps. MexT, a LysR-type transcriptional regulator, functions as a transcriptional activator of the MexEF-OprN efflux system. MexT consists of an N-terminal DNA-binding domain and a C-terminal regulatory domain (RD). Little is known regarding MexT ligands and its mechanism of activation. We elucidated the crystal structure of the MexT RD at 2.0 Å resolution. The structure comprised two protomer chains in a dimeric arrangement. MexT possessed an arginine-rich region and a hydrophobic patch lined by a variable loop, both of which are putative ligand-binding sites. The three-dimensional structure of MexT provided clues to the interacting ligand structure. A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the mexEF-oprN promoter. Our findings enhance the understanding of the regulation of MexT-dependent activation of efflux pumps.

• Journal of Sensor Science and Technology
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• v.8 no.2
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• pp.139-147
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• 1999

In this paper, we present the new angular velocity pick-off method for DTG (dynamically tuned gyroscope) which is widely used in various inertial navigation systems and motion control systems. In case of the external angular velocity input, the proposed scheme can make a smaller tilt-angle rather than that of conventional PI method in the transient and steady state because it has an additional inner rebalance loop with a mathematical model of the real gyroscope. So, without any mechanical redesign of the DTG, its dynamic range can be enlarged by the proposed method. The theoretical analysis and simulation model of DTG with the proposed scheme are given. Finally, the proposed scheme is verified.

• Molecules and Cells
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• v.41 no.6
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• pp.545-552
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• 2018

Spleen tyrosine kinase (SYK) is a cytosolic non-receptor protein tyrosine kinase. Because SYK mediates key receptor signaling pathways involving the B cell receptor and Fc receptors, SYK is an attractive target for autoimmune disease and cancer treatments. To date, representative oral SYK inhibitors, including fostamatinib (R406 or R788), entospletinib (GS-9973), cerdulatinib (PRT062070), and TAK-659, have been assessed in clinical trials. Here, we report the crystal structures of SYK in complex with two newly developed inhibitors possessing 4-aminopyrido[4,3-D]pyrimidine moieties (SKI-G-618 and SKI-O-85). One SYK inhibitor (SKI-G-618) exhibited moderate inhibitory activity against SYK, whereas the other inhibitor (SKI-O-85) exhibited a low inhibitory profile against SYK. Binding mode analysis indicates that a highly potent SYK inhibitor might be developed by modifying and optimizing the functional groups that interact with Leu377, Gly378, and Val385 in the G-loop and the nearby region in SYK. In agreement with our structural analysis, one of our SYK inhibitor (SKI-G-618) shows strong inhibitory activities on the ${\beta}$-hexosaminidase release and phosphorylation of SYK/Vav in RBL-2H3 cells. Taken together, our findings have important implications for the design of high affinity SYK inhibitors.

• Journal of Advanced Marine Engineering and Technology
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• v.31 no.6
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• pp.699-706
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• 2007

The cooling heat transfer coefficient and pressure drop of $CO_2$(R-744) in helical coil copper tubes were investigated experimentally The main components of the refrigerant loop are a receiver, a variable-speed pump, a mass flow meter. a pre-heater and a inclined helical coil type gas cooler (test section). The test section consists of a smooth copper tube of 2.45 and 4.55mm inner diameter The refrigerant mass fluxes were varied from 200 to $600 [kg/m^2s]$ and the inlet pressures of 9as cooler were 7.5 to 10.0 [MPa]. The heat transfer coefficients of $CO_2$ in helical coil tubes increase with the increase of mass flux and gas cooling pressure of $CO_2$. The pressure drop of $CO_2$ in the gas cooler shows a relatively food agreement with those Predicted by Ito's correlation developed for single-phase in helical coil tubes. Though a few correlation available with the data. the local heat transfer coefficient of $CO_2$ agrees well with those presented by Pitla et al. among the predictions. However at the region near pseudo-critical temperature. the experiment data indicate higher values than the Pitla et al. correlation.

• Journal of Magnetics
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• v.4 no.1
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• pp.33-37
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• 1999

Unidirectional magnetic anisotropy field ($H_an$) was investigated for thin films of $CdCr{2-2x}In_{2X}Se_4 (0$\leq$x$\leq$0.2). This anisotropy originates from the microscopic anisotropic Dzyaloshinskii-Moriya (DM) interaction which arise from the spin-orbit scattering of the conduction electrons by the nonmagnetic impurities. This interaction maintains the remanent magnetization in the direction of the initial applied field. Then the single easy direction of the magnetization is parallel to the direction of the magnetic field. The anisotropy produced by field cooling is unidirectional I.e. the spins system deeps some memory of the cooling field direction. The chalcogenide spinel of$ CdCr_{2-2x}In){2X}Se_4$belongs to the class of the magnetic semiconductors. The magnetic disordered state is obtained when ferromagnetic structure is diluted by In. Then we have the mixed phase characterised by coexistence the magnetic long range ordering (IFN-infinite ferromagnetic network) and the spin glass order (Fc-finite clusters). The total magnetic anisotropy energy depends on the state of magnetic ordering. In our study we concentrated on the magnetic state with reentrant transition and spin glass state. The polycrystalline $ CdCr_{2-2x}In){2X}Se_4$ thin films were obtained by rf sputtering technique. We applied the ferromagnetic resonance (FMR) and M-H loop techniques for determining the temperature composition dependencies of Han. From the experimental data, we have found that Han decreases almost linearly when temperature is increased and in the low temperature is about three times bigger at SG state with comparison to the state with REE.

• Journal of Advanced Marine Engineering and Technology
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• v.31 no.6
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• pp.707-714
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• 2007

The heat transfer coefficient and Pressure drop during gas cooling process of $CO_2$ (R-744) in inclined helical coil copper tubes were investigated experimentally. The main components of the refrigerant loop are a receiver. a variable-speed pump. a mass flow meter, a pre-heater and a inclined helical coil type gas cooler (test section). The test section consists of a smooth copper tube of 2.45mm inner diameter. The refrigerant mass fluxes were varied from 200 to $600[kg/m^2s]$ and the inlet Pressures of gas cooler were 7.5 to 10.0 [MPa]. The heat transfer coefficients of $CO_2$ in the inclined helical coil tubes increases with the increase of mass flux and gas cooling pressure of $CO_2$. The pressure drop of $CO_2$ in the gas cooler shows a relatively good agreement with those Predicted by Ito's correlation developed for single-phase in a helical coil tube. The local heat transfer coefficient of $CO_2$ agrees well with the correlation by Pitla et al. However, at the region near pseudo-critical temperature. the experiments indicate higher values than the Pitla et al. correlation. Therefore. various experiments in the inclined helical coil tubes have to be conducted and it is necessary to develop the reliable and accurate prediction determining the heat transfer and pressure drop of $CO_2$ in the inclined helical coil tubes.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.19 no.9
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• pp.640-646
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• 2007

The cooling heat transfer coefficient of $CO_2$ (R-744) for tube and coil diameter (CD), inclined angle of tube and coil pitch of inclined helical coil type copper tubes were investigated experimentally. The main components of the refrigerant loop are a receiver, a variable-speed pump, a mass flow meter, a pre-heater and a inclined helical coil type gas cooler (test section). The test section consists of a smooth copper tube of 2.45 and 4.55 mm inner diameter (ID). The refrigerant mass flukes were varied from 200 to 800 [$kg/m^2s$] and the inlet pressures of gas cooler were 7.5 to 10.0 [MPa]. The heat transfer coefficients of $CO_2$ in inclined helical coil tube with 2.45 mm ID are $5{\sim}10.3%$ higher than those of 4.55 mm. The heat transfer coefficients of 41.35 mm CD are $8{\sim}32.4%$ higher than those of 26.75 mm CD. Comparison between $45^{\circ}\;and\;90^{\circ}$ of coil angle, the heat transfer coefficients of $45^{\circ}$ are higher than those of $90^{\circ}$. For coil pitch of gas cooler, the heat transfer coefficients of inclined helical coil gas cooler with coil pitch of 5 mm are similar to those of 10 and 15 mm.

• Maritime Security
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• v.1 no.1
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• pp.215-240
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• 2020

In 2017, the U.S. DARPA coined 'mosaic warfare' as a new way of warfighting. According to the Timothy Grayson, director of DARPA's Strategic Technologies Office, mosaic warfare is a "system of system" approach to warfghting designed around compatible "tiles" of capabilities, rather than uniquely shaped "puzzle pieces" that must be fitted into a specific slot in a battle plan in order for it to work. Prior to cover mosaic warfare theory and recent development, it deals analyze its background and several premises for better understanding. The U.S. DoD officials might acknowledge the current its forces vulnerability to the China's A2/AD assets. Furthermore, the U.S. seeks to complete military superiority even in other nation's territorial domains including sea and air. Given its rapid combat restoration capability and less manpower casualty, the U.S. would be able to ready to endure war of attrition that requires massive resources. The core concept of mosaic warfare is a "decision centric warfare". To embody this idea, it create adaptability for U.S. forces and complexity or uncertainty for the enemy through the rapid composition and recomposition of a more disag g reg ated U.S. military force using human command and machine control. This allows providing more options to friendly forces and collapse adversary's OODA loop eventually. Adaptable kill web, composable force packages, A.I., and context-centric C3 architecture are crucial elements to implement and carry out mosaic warfare. Recently, CSBA showed an compelling assessment of mosaic warfare simulation. In this wargame, there was a significant differences between traditional and mosaic teams. Mosaic team was able to mount more simultaneous actions, creating additional complexity to adversaries and overwhelming their decision-making with less friendly force's human casualty. It increase the speed of the U.S. force's decision-making, enabling commanders to better employ tempo. Consequently, this article finds out and suggests implications for Korea armed forces. First of all, it needs to examine and develop 'mosaic warfare' in terms of our security circumstance. In response to future warfare, reviewing overall force structure and architecture is required which is able to compose force element regardless domain. In regards to insufficient defense resources and budget, "choice" and "concentration" are also essential. It needs to have eyes on the neighboring countries' development of future war concept carefully.

• Journal of Embryo Transfer
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• v.20 no.2
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• pp.163-168
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• 2005

The objective of this study was to produce superior cows by sexual distinction of embryos collected from the donor with pedigree information. Collected embryos distinguished by biopsy using punching or bisection methods and Loop-mediated isothermal amplification of sex-determined DNA for sexing. Six embryos predicted as female were transplanted to recipients and then 2 pregnant cows were normally delivered of calves at 278 and 285 days after embryo transfer. Birth weights of calves named Barani and Borani were 18kg and 25kg, respectively. Adjusted body weights for 90 days were 61.1kg and 88.8kg, respectively. Average daily gains were 0.48kg and 0.71kg, respectively.

• Journal of the Korean Institute of Gas
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• v.11 no.3
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• pp.1-6
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• 2007

The heat transfer coefficient and pressure drop during gas cooling process of $CO_2$ (R-744) in inclined helical coil copper tubes were investigated experimentally. The main components of the refrigerant loop are a receiver, a variable-speed pump, a mass flow meter, a pre-heater and a inclined helical coil type gas cooler (test section). The test section consists of a smooth copper tube, which is specified as the inner diameter of 4.55 mm. The refrigerant mass fluxes were varied from 200 to $600kg/m^2s$ and the inlet pressures of gas cooler were done 7.5 to 10.0 (MPa). The heat transfer coefficients of $CO_2$ in the inclined helical coil tubes increase with the increase of mass flux and gas cooling pressure of $CO_2$. The pressure drop of $CO_2$ in the gas cooler shows relatively good coincidence with those predicted by Ito's correlation developed for single-phase in a helical coil tube. The local heat transfer coefficient of $CO_2$ is well coincident with the correlation by Pitla et al. However, at the region near pseudo-critical temperature, the experiments indicate higher values than the Pitla et al. correlation.