• Title/Summary/Keyword: R.P.R. Method

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Cloning of 17S-Ribosomal RNA Gene from the Hygromycin Resistant Tetrahymena thermophila (Hygromycin내성 Tetrahymena thermophila의 17S-Ribosomal RNA유전자의 Cloning)

  • 홍용기
    • Microbiology and Biotechnology Letters
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    • v.14 no.2
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    • pp.133-137
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    • 1986
  • 17S-ribosomal RNA gene from the hygromycin resistant protozoan Tetrahymena thermophila hmr 3 was cloned on E. coli vector pBR 322 as part of study to work the 17S-rRNA structure and the mechanism of hygromycin resistance. The 17S-rDNA was inserted into the Hind 111 site of pBR 322. The clones having recombinant plasmid were selected by the method of colony hybridization with a 17S-rDNA probe of wild type B1868. The orientation of 17S-rDNA insert was located near the tetracycline resistant gene of pBR 322 in a clone 5-19 with the recombinant plasmid.

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Usefulness of Registration in the Evaluation of Parkinson′s Disease (영상 융합하여 분석한 파킨슨씨병 환자영상의 유용성)

  • 주라형;김재승;문대혁;최보영;서태석
    • Progress in Medical Physics
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    • v.14 no.4
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    • pp.268-278
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    • 2003
  • Purpose:The aim of this study was to evaluate the striatal binding ratio, the anterior/posterior ratio and reproducibility using a template based registration method using the standard MR template as a replacement for each patients MR image. Materials and Methods:This study analyzed the 123I IPT SPECT images of 30 patients with IPD, who were subdivided into 17 patients (56.6$\pm$10.8 yr, M/F : 8/9.) with mild IPD, and 13 patients (56.4$\pm$11.1 yr, M/F : 8/5) with severe IPD. In addition, 11 normal controls (57.8$\pm$14.4 yr, M/F : 4/7) were also analyzed. The ROIs were positioned manually in the same slice showing the highest striatal activity using the traditional manual method, whereas those were positioned automatically in a mid striatal slice of the SPECT image coregistered to the standard T1 weighted MR template. Results : The specific binding ratio (SBR) obtained using the template based registration method strongly correlated with those using the manual method in all groups : normal controls (r=0.85, P<0.001), mild IPD (r=0.84, P<0.001) and severe IPD (r=0.7, P=0.01). The SBRs obtained using both methods were significantly different among the three groups (P=0.05) and the SBRs obtained by the template based registration method were higher than those by the manual method (P=0.05) in all three groups. The APRs obtained by the template based registration correlated with those using manual method in only mild IPD (r=0.72, P=0.0). The APRs obtained by the template based registration method were significantly different from the normal controls and those with mild or severe IPD (P<0.05), whereas those obtained using the manual method were not significantly different among the three groups (P>0.1). The reproducibility (rmsCV) of the template based registration method was 7.2% (normal controls:5.2%, mild IPD:4.2%, severe IPD:10.8%), whereas the reproducibility of the manual method was 31% (normal controls:19.7%, mild IPD:21.7%, severe IPD:46.2%). Conclusion:These results show that the use of $^{123}$ I-IPT SPECT for assessing IPD is affected by the methods used to position the striatal ROI. The template based registration method using the standard MR template can be useful in diagnosing IPD and assessing the disease severity with a high reproducibility. Therefore, the template based registration method appears to be a good replacement for the manual method.

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A Study of Property F.R.P Structure Strength According to the Direction of Lay-up in the Small Ship (적층 방향에 따른 F.R.P 구조강도특성에 관한 연구)

  • 고재용;배동균;윤순동
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2002.11a
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    • pp.101-105
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    • 2002
  • FRP(Fiber glass reinforced plastics) is compound with materials, which are created to combine each other materials, of which nature of mechanical and chemical are different. Even though the weight and the thickness are identic, its physical figure of characteristic changes with consisting of lay-up and work situation. It is also a method of creating after manufacturing of mould. It has feature that manufacturing of FRP runs parallel design of material with design of structure simultaneously. The rule of FRP structure is distinguished from the length of a ship and it is hard to catch the feature of structure mechanics due to identical formula and figure used for it regardless of the shape of a ship or the speed. This studying, basing on a small FRP ship, will show te fundamental data needed to design of structure analysing the feature of intensity with direction, the method of Lay-up, and the characteristic of materials of FRP.

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Factors Affecting Spirituality in Nursing Students (간호대학생의 영성에 미치는 영향 요인)

  • Yun, So Hyeon;Jun, Won Hee
    • Journal of Korean Academic Society of Home Health Care Nursing
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    • v.26 no.1
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    • pp.64-71
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    • 2019
  • Purpose: This study investigated the factors affecting spirituality in nursing students. Method: A total of 152 nursing students participated in the study. Data collection was conducted from October to November 2018. The data were analyzed using descriptive statistics, t-tests, analysis of variance, Pearson's correlation coefficient, and multiple stepwise regression via SPSS WIN 25.0. Results: The mean scores for empathy, gratitude, positive affect, negative affect, and spirituality were all average. However, there were significant differences in mean scores for spirituality according to satisfaction with major (F=5.88, p=.003), study grade (F=10.17, p<.001). Spirituality was found to be correlated with fantasy (r=.17, p<.001), empathic concern (r=.41, p<.001), perspective taking (r=.42, p<.001), personal distress (r=-.31, p<.001), gratitude (r=.65, p<.001), positive affect (r=.52, p<.001), and negative affect (r=-.30, p<.001). Significant predictors of spirituality included gratitude (${\beta}=.38$, p<.001), study grade (${\beta}=.34$, p=.009), positive affect (${\beta}=.29$, p<.001), and perspective taking (${\beta}=.16$, p=0.37), one of the sub-dimensions of empathy. Conclusion: To improve spirituality among nursing students, nursing educators should develop educational intervention programs to promote gratitude, study grades, positive affect, and perspective taking.

Economical Values of Gage R&R Parameters (경제적인 Gage R&R 계수)

  • Park, Sung-Hun;Kang, Chang-Wook
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.35 no.3
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    • pp.129-135
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    • 2012
  • Companies strive for quality improvement and use process data obtained through measurement process to monitor and control the process. Measurement data contain variation due to error of operator and instrument. The total variation is sum of product variation and measurement variation. Gage R&R is for repeatability and reproducibility of measurement system. Gage R&R study is usually conducted to analyze the measurement process. In performing the gage R&R study, several parameters such as the appropriate number of operators (o), sample size of parts (p), and replicate (r) are used. In this paper we propose how to determine the optimal combination of number of operators (o), sample size of parts (p), and replicates (r) considering measurement time and cost by statistical method.

The relationships between lead exposure indicies and urinary δ-ALA by HPLC and colorimetric method in lead exposure workers (연노출근로자에 있어서 흡광광도법과 HPLC법에 의한 요중 δ-ALA 배설량과 연노출지표들 간의 관련성)

  • Ahn, Kyu-Dong;Lee, Sung-Soo;Hwangbo, Young;Lee, Gab-Soo;Yeon, You-Yong;Kim, Yong-Bae;Lee, Byung-Kook
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.6 no.1
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    • pp.77-87
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    • 1996
  • In order to compare the difference of the measurement of delta aminolevulinic acid(${\delta}$-ALA) in urine between HPLC method(HALA) and colorimetric method(CALA), and also to provide useful information for the new diagnostic criteria of ${\delta}$-ALA in urine in lead poisoning, if at all possible in the future, authors studied 234 male lead workers who were selected from 7 storage battery factories, 3 secondary smelting industries, and 2 litharge making industries. Study subjects were selected on the basis of blood Zinc protoporphyrin(ZPP) level from low to high concentration to cover wide range of lead exposure. Study variables for this study were ${\delta}$-ALA measured by two different methods, blood lead(PbB), and blood ZPP. The results were as follows: 1. There was very high correlation between ${\delta}$-ALA measured by two method(r = 0.989 : HALA = -0.8194 + 0.8110 ${\times}$ CALA), but the value of CALA was measured about 2mg/L greater than HALA. 2. While the correlations of ${\delta}$-ALA by two method with blood lead and blood ZPP were 0.46 and 0.37 respectively, they were increased to 0.63 and 0.57 if ${\delta}$-ALA values were log-transformed. 3. Simple linear regression of ${\delta}$-ALA measured by two method on ZPP were as follows: CALA = 2.0421 + 0.0341 ${\times}$ ZPP ($R^2=0.1385$ p = 0.0001) HALA = 0.8006 + 0.0280 ${\times}$ ZPP ($R^2=0.1389$ p = 0.0001) 4. Simple linear regression of ${\delta}$-ALA measured by two method on PbB were as follows: CALA = - 0.4134 + 0.1545 ${\times}$ PbB ($R^2=0.2085$ p = 0.0001) HALA = -1.2893 + 0.1287 PbB ($R^2=0.2154$ p = 0.0001), 5. Simple linear regression of log-transformed ${\delta}$-ALA by two method on ZPP and PbB were as follows: logHALA = 0.3078 + 0.0060 ZPP ($R^2=0.3329$ p = 0.0001) logCALA = 1.0189 + 0.0044 ZPP ($R^2=0.3290$ p = 0.0001) logHALA = -0.0221 + 0.0246 PbB ($R^2=0.4046$ p = 0.0001) logCALA = 0.7662 + 0.0184 PbB ($R^2=0.4108$ p = 0.0001) 6. The cumulative percent of colorimetric method to detect lead workers whose value of PbS and ZPP were over screening level such as $40{\mu}/dl$ and $100{\mu}/dl$ respectively was higher than HPLC method if cut-off level of ${\delta}$-ALA for screening of lead poisoning was 5 mg/L. But if cut-off level of ${\delta}$-ALA measured by HPLC was reduced to 3 mg/L which is compatible to 5 mg/L of ${\delta}$-ALA measured by colorimetric method, there were good agreement between two methods and showed dose-response relationship with other lead exposure indices such as PbB and ZPP.

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Design and Construction of Analysis System using R (R 을 이용한 분석 시스템 구축 방안 및 설계)

  • Kwon, Youngoh;Jin, Byoungsam;Bae, Youngchul
    • Journal of Platform Technology
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    • v.6 no.1
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    • pp.9-16
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    • 2018
  • In recent years, many analysts have been using Python and R to analyze data using efficient analysis and statistics. However, it is difficult to solve the requirement to express various analyzes in real time through the web system using only two languages. In addition, it is possible to confirm that the extracted P-Value is different from the value in R because there is no corresponding algorithm in Java or the corresponding source is not verified. Therefore, in this paper, we propose a method to apply analyst 's analysis algorithm contents directly to his system using R in other languages.

Spatiotemporal Gait Parameters That Predict the Tinetti Performance-Oriented Mobility Assessment in People With Stroke

  • Jeong, Yeon-gyu;Kim, Jeong-soo
    • Physical Therapy Korea
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    • v.22 no.4
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    • pp.27-33
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    • 2015
  • The purpose of this study was to find which spatiotemporal gait parameters gained from stroke patients could be predictive factors for the gait part of Tinetti Performance-Oriented Mobility Assessment (POMA-G). Two hundred forty-six stroke patients were recruited for this study. They participated in two assessments, the POMA-G and computerized spatiotemporal gait analysis. To analyze the relationship between the POMA-G and spatiotemporal parameters, we used Pearson's correlation coefficients. In addition, multiple linear regression analyses (stepwise method) were used to predict the spatiotemporal gait parameters that correlated most with the POMA-G. The results show that the gait velocity (r=.67, p<.01), cadence (r=.66, p<.01), step length of the affected side (r=.49, p<.01), step length of the non-affected side (r=.53, p<.01), swing percentage of the non-affected side (r=.47, p<.01), and single support percentage of the affected side (r=.53, p<.01) as well as the double support percentage of the non-affected side (r=-.42, p<.01) and the step-length asymmetry (r=-.64, p<.01) correlated with POMA-G. The gait velocity, step-length asymmetry, cadence, and single support percentage of the affected side explained 67%, 2%, 2%, and 1% of the variance in the POMA-G, respectively. In conclusion, gait velocity would be the most predictive factor for the POMA-G.

Syntheses and Characteristics of R-P Phase $Ln_{1-x}Sr_{2-x}Mn_2O_7$(Ln=La, Pr, Gd, Eu, Er, Nd, Sm) (R-P Phase $Ln_{1-x}Sr_{2-x}Mn_2O_7$(Ln=La, Pr, Gd, Eu, Er, Nd, Sm)의 합성 및 특성연구)

  • 송민석;서상일;이재열
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1999.05a
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    • pp.337-340
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    • 1999
  • Since the first reports of CMR(colossal magnetoresistance) effects in some single crystal R-P Phase Ln$_{1-x}$ Sr$_{2-x}$Mn$_{2}$O$_{7}$ 1996. many researches have been carried out to find optimum compositions and processing conditions in this system. In this study, layered perovskite R-P Phase Ln$_{1-x}$ Sr$_{2-x}$Mn$_{2}$O$_{7}$ (x=0.4, Ln=-La, Eu, Gd, Nd, Pr, Sm) phases were synthesized by solid state reaction and their structures were refined by Rietveld method. Electrical and magnetic properties were measured drown to 20K and compare with those of R-P Phase Ln$_{1-x}$ Sr$_{2-x}$Mn$_{2}$O$_{7}$ Phases.es.es.es.es.es.

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