• Journal of Life Science
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• v.19 no.6
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• pp.799-803
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• 2009

The killing of male germ cells by radiation and other toxicants has recently been attributed to apoptosis, but a critical evaluation of the presence of the different features of apoptosis in each epithelial stage has not been performed. In this study, mouse testes exposed to radiation were examined by light microscopy and terminal transferase-mediated end labeling (TUNEL) with periodic acid-Schiff (PAS) stains to determine whether the cells were apoptotic according to several criteria. Apoptosis was easily recognized by the presence of peroxidase-stained, entirely apoptotic bodies. In the TUNEL-positive cells or bodies, the stained products correlated precisely with the typical morphologic characteristics of apoptosis as seen at the light microscopic level. The changes that occurred from 0 to 24 hours after exposing the mice to 2 Gy of gamma-rays (2 Gy/min) were examined. The numbers of apoptotic cells reached a peak at 12 hours after irradiation and then declined. The mice that received 0-8 Gy of gamma-rays were examined 8 hours after irradiation. Dose-response relationships were generated for each stage of the epithelial cycle by counting TUNEL-positive cells. The dose-response curves were linear- quadratic [y=(-0.014${\pm}$0.009)$D^{2}$+(0.31${\pm}$0.697)D+0.3575. Where y=the number of apoptotic cells per seminiferous tubule, and D=the irradiation dose in Gy, $r^{2}$=0.9] and there was a significant relationship between the frequency of apoptotic cells and the radiation dose. Although the maximum response was produced by 8 Gy, even 0.5 Gy induced marked changes. These changes were most pronounced in B spermatogonia of stage V and the spermatocyte at the mitotic cells of stage XII.

• The Journal of Internal Korean Medicine
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• v.28 no.3
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• pp.464-472
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• 2007

Objectives : Nowadays the combined use of oriental herbal medicines and western biomedical medicines has been prevalent but controversial. Warfarin has been much reported to interact with some herbal medicines so that it influences prothrombin time(PT) & international normalized ratio(INR). This study was aimed to examine how much warfarin interacts with herbal medicines during treatment of stroke patients Methods : This was a retrospective case control study of 53 patients whowere treated with concomitant treatment of herbal medicines & warfarin. They were within normal limit in liver function, renal function, hematocrit, hemoglobin, and platelet count at first admission lab. We classified them into 2 classes: study group (taking herbal medicines including Panax ginseng, Angelica sinensis, Zingiber officinale, Salvia miltiorrhiza that were reported to interact with warfarin to impact PT (INR) and control group (taking other herbal medicines). We followed up PT (INR) at 5-10 days interval with AST, ALT, BUN, creatinine, hematocrit, hemoglobin, and platelet count. Results : AST, BUN, creatinine, hemoglobin, hematocrit, and platelet count were not changed significantly between first and final tests during the admission period. Only ALT decreased significantly in the control group. Neither baseline nor peak PT (INR) was significantly different between the groups. However, only warfarin dose was significantly correlated with PT and INR (r=0.810, r=0.798, p<0.01). Conclusions : It was concluded that PT(INR) was not influenced with herbal medicines and warfarin but by far dependent on warfarin dose in stroke patients restricted with normal liver function, renal function, and hematocrit, hemoglobin, and platelet count. Further prospective study is needed on larger samples to conclude that the combined therapy of herbal medicines and warfarin is safe.

• Analytical Science and Technology
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• v.29 no.6
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• pp.277-282
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• 2016

British Pharmacopoeia (BP 2013), the United States Pharmacopoeia (USP 39) and the Korean Pharmacopoeia (KP XI) contain monographs for the quality control of raw diosmin using high performance liquid chromatography (HPLC). However, official monographs detailing pharmaceutical formulations for diosmin are not available in foreign pharmacopoeias. In the KP XI, ultraviolet-visible (UV-Vis) spectroscopy-which is less specific than HPLC-is reported for the testing of diosmin capsules. In this study, we present an alternative HPLC assay for such testing that is more specific than UV-Vis methods. Method validation was performed to determine linearity, precision, accuracy, system suitability, and robustness. The linearity of calibration curves in the desired concentration range was high ($r^2$>0.999), while the RSDs for intra- and inter-day precision were 0.15-0.29 % and 1.05-1.74%, respectively. Accuracies ranged from 101.2-103.2 %, while the retention time and peak area RSDs were 0.37 % and 0.06 %, respectively. Additionally, the plate number and asymmetry factor values for diosmin were 3591.293 and 1.35, respectively. Since the intermediate-precision and robustness of the assay were satisfactory, this method will be a valuable addition to the Korean Pharmacopoeia (KP XI).

• Journal of Bio-Environment Control
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• v.11 no.4
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• pp.163-167
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• 2002

This study was carried out to investigate the effect of minimum night temperature in the PE house on growth of seedlings of Pinus densiflora and Betula platyphylla in winter season. The experiment was performed with three minimum night temperature regimes,5~8$^{\circ}C$, 10~13$^{\circ}C$ and 15~18$^{\circ}C$. The temperature regimes were maintained for 8 weeks (to April 6) after germination peak, and then were broken by increasing natural temperature. The temperature did not affect the germination rate but delayed the germination by 10 days. Height, root collar diameter growth, and dry weight of two species were reduced by relatively low night temperature. The reduction was more obvious in the dry weight of above the ground measured at 8 weeks after germination. The seedlings grown for 8 weeks at 5~8$^{\circ}C$ showed the lowest T/R ratio. It was also observed that the height growth response in Betula platyphylla seedlings to relatively low temperature was more sensitive than Pinus densiflora.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.49-53
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• 1998

For the effective utilization of by-products from pollack, Theragra chalcogramma, processing, low salt fermented pollack tripe were produced and changes in taste compounds of low salt fermented pollack tripe during the controlled freezing point aging were investigated. No significant changes in contents of moisture, crude protein, crude lipid and ash were found during aging. The volatile base nitrogen of low salt fermented pollack tripe increased steadily as aging progressed. Contents of amino nitrogen and nitrogenous compounds of extract reached a peak in 50 days of aging, as 190.7 mg% and 2085.8 mg%, respectively. Inosine(HxR) of low salt fermented pollack tripe increased as controlled freezing point aging progressed for 60 days. 18 species of amino acid were detected in raw pollack tripe and law salt fermented pollack tripe aged for 50 days. The major amino acids were tyrosine, phenylalanine, histidine, arginine, lycine, leucine, aspartic acid and glutamic acid in raw pollack tripe. After 50 days aging, increase of a series of bitter taste amino acid such as methionine, valine and isoleucine were higher than other amino acids.

• Journal of the Korean Geotechnical Society
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• v.33 no.12
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• pp.35-44
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• 2017

A large amount of recycled aggregates was produced and crushed from old buildings and pavements. In this study, when these aggregates are re-used in subbase or subgrade materials in near construction sites, their engineering characteristics caused by crushing are investigated in terms of permeability and shear strength. Three different sizes of aggregates (31.5-45.0 mm, 19.0-31.5 mm, 9.5-19.0 mm) and their mixtures, a total of 7 types of aggregates were used in compaction tests (modified D and B methods). After compaction tests, aggregates were sieved and analyzed with four different breakage factors ($B_{15}$, $C_c$, $B_{10}$, $B_r$). The D compaction method gave 2.0-8.0 times more crushable than B compaction method. The breakage factors for the largest size aggregate was 1.4-3.0 times higher than those of the smallest size aggregate. For aggregates with 5.6-9.5 mm sizes, the samples were prepared with $B_{15}$ of 1, 3, 10, 20, 30, 50, 60, and 70 for permeability and direct shear tests. As $B_{15}$ increased, the hydraulic conductivity decreased up to 1/22 for $B_{15}=50$. As $B_{15}$ increased from 1 to 50, the peak friction angle increased from $46.1^{\circ}$ to $54.5^{\circ}$. On the other hand, the friction angle decreased after $B_{15}=60$.

• Korean Journal of Agricultural Science
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• v.22 no.2
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• pp.151-155
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• 1995

Two types of modified celluloses which contain trinitrophenyl groups as chromophore were synthesized from carboxymethyl cellulose Whatman CM 70 and CM 32. Diaminoethyl groups were added to the CM 70 and CM 32 to make DAE-CM celluloses and then the DAE-CM groups were substituted by 2,4,6-trinitrophenyl groups to produce TNP-celluloses. Average particle size of the TNP-cellulose from CM 32 was $44.6{\pm}9.6{\mu}m$ in diameter and $127.9{\pm}22.5{\mu}m$ in length, which was much smaller than those from CM 70, however its TNP-moiety per gram determined by using the molar extinction coefficient $1.33{\times}10^4$ of ${\varepsilon}$-TNP-lysine at 345 nm, was 0.68 millimoles, which was 5.6-fold greater than those from CM 70. The absorption spectrum of TNP-oligosaccharides which were the soluble products of TNP-celluloses by a cellulase preparation Onozuka R-10, showed a maximal peak at 344 nm. Increases in the absorbance during hydrolysis were linear with the enzyme concentration, and the differences of slope values between two types of TNP-celluloses that the more semsitive assay could be achieved by using those from CM 32 as substrate at the low range of the enzyme concentration.

• The Korean Journal of Pharmacology
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• v.29 no.1
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• pp.121-130
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• 1993

This study was done to classify the proteins involved in the specific phosphorylation using the rat peripheral blood lymphocytes (rPBL) stimulated with mitogens, phorbol 12-myristate 13-acetate (PMA) and concanavalin A (Con A). The lymphocytes were incubated with $^{32}P-orthophosphate$ before PMA or Con A stimulation. The migration patterns of the phosphorylated proteins of mitogen-treated rPBL in two dimensional electrophoretic fields were analyzed after autoradiography. The stimulation of the lymphocytes with PMA and Con A increased the phosphorylation of thirteen protein fractions. The phosphorylation intensities of the protein spots differ to the treatments of the cells with specific kinase inhibitors, H-7 and W-7. These protein fractions were grouped into 3 classes, namely, PKC-mediated, CaM kinase-mediated, and other kinase mediated proteins. The effect of the duration of the stimulation on the phosphorylated behaviors occurred concurrently, not sequentially, although each individual protein fraction had a different time for the peak phosphorylation during the stimulation period upto 30 minutes. The phosphoproteins found in the cytosolic soluble fraction were phosphorylated prior to those in the pellet, whose phosphorylations were sustained at a high level for over 10 minutes. The above results suggest that the early events in lymphocyte activation involve 3 different sets of proteins which are phosphorylated by CaM kinase, PKC and other kinases, and those kinases do not work sequentially, but rather, independently or cooperatively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.3
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• pp.245-253
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• 2001

To investigate the effects of hydromechanical and textural characteristics of sediment deposits on the cultivation of Manila clam, Ruditapes philippinarum surface and sub-surface core sediments were collected seasonally in Gomso tidal flat. Grain size distribution were analyzed to investigate the annual variation of sediment texture. In winter unimodal distribution of grain size with the peak at $5\phi$ is dominant However, during the summer sediment texture become a little bit coarser and grain size distribution shows the peaks at $4\~5 \phi$. Optimum sediment texture for the cultivation of manila clam, R. philippinarum was found to be sandy silt in which mean Brain size was between 4 and $5 \phi$ with the sand content less than $50\%$ and clay content of $5\~10\%$. Mechanical and hydrological characteristics of sediment deposits were also studied in the laboratory and the results were applied to the numerical simulation for the behavior of surface sediment subjected to the cyclic loading from sea-water level change. Results of numerical simulation illustrate that the permeability of sediment had to be maintained in the range of $10^{-11}\sim10^{-12}m^2$ to ensure the proper sedimentological environment for the cultivation of manila clam, R. philippinarum. The deposits of virtually impermeable mud layer, with the threshold thickness of 4 cm, would be very hazardous to clam habitat.

• Korean journal of applied entomology
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• v.17 no.1 s.34
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• pp.41-47
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• 1978

Pheromones of several insect species were evaluated in a screening program in terms of their usefulness in pest forecasting and control in Korea. Species included striped rite borer (Chilo suppressalis) and tortricid moths, which attack deciduous fruit, and colding moth. The pheromone of striped rice borer was supplied through the courtesy of the Tropical Products Institute, London. Pheromones of other species were obtained from Cornell University and the Zoecon Corporation of Palo Alto, California. 'rho results of this experiment were as follows: 1. Live traps containing virgin striped rice borer female moths were more effective in attracting male moths than were the pheromone traps. 2. Since the effectiveness of the striped rice borer pheromone decreased dramatically with time, it was difficult to estimate the peak tine of the borer emergence. 3. The primary species trapped in deciduous fruit orchards was the oriental fruit moth, Grapholitha molesta. The traps were baited with the phermones, OFM and LAW. 4. Several moth species were trapped with OBLR. RBLR. SPAR and ArcM phermones but few were trapped with the remaining eight tortricid pheromones. 5. The following tortricid pheromones might be useful for forecasting the species given: OFM, LAW : Grapholitha molesta OBLR : Archips breviprecanus OBLH, RBLR, ArcM : Archippus coreensis Archips fuscocupreanus Hoshinoa longicellana SPAR, TBM ; Phyroderces sp.