• 보존과학회지
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• 제34권4호
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• pp.259-271
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• 2018

Outdoor copper alloy is exposed to the atmospheric environment, accelerating corrosion progress compared with indoor copper alloy. In order to prevent corrosion, the outdoor copper alloy is coated with wax to block external corrosion factors. However, corrosion of the inside of the coating film is highly likely to continue without the internal corrosion prevention treatment. B.T.A, which is used as a copper alloy water-soluble corrosion inhibitor, has a high possibility of being harmful to the human body and is mainly used to treat excavated artifacts. This study had selected the water-soluble corrosion inhibitor, which was easier to use than the existing wax and B.T.A being used in corrosion inhibition treatment for outdoor copper alloy. A comparative study was conducted on B.T.A, which is a water-soluble corrosion inhibitor used on excavated artifacts, and $VCI^{(R)}$, $Rus^{(R)}$, and L-cys, an amino acid corrosion inhibitor, used for tin bronze test pieces. The experimental method was conducted for a certain period of time with the salt, acid, and air pollution affecting the corrosion of outdoor copper alloy. Based on experiment results, it was concluded that the best water - soluble copper alloy corrosion inhibitor in the atmospheric environment is $VCI^{(R)}$. and it could be considered to be applied in replacement of B.T.A due to its low harmfulness. In addition, $VCI^{(R)}$ is judged to serve as a corrosion inhibitor for outdoor copper alloy because it showed the best result even in the outdoor exposure test which is a real atmospheric environment.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7603-7609
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• 2014

Glutathione is a thiol compound that plays an important role in the antioxidant defense system of the cell and its deficiency leads to an increased susceptibility to oxidative stress and, thus, progression of many disease states including head and neck cancer. In the present study, alterations of glutathione levels were investigated in study cohort of 500 samples (cohort 1 containing 200 head and neck cancer blood samples along with 200 healthy controls and cohort II with 50 head and neck squamous cell carcinoma tissue samples along with 50 control tissues) by high performance liquid chromatography. The results indicated that mean blood glutathione levels were significantly reduced in head and neck cancer patients (p<0.001) compared to respective controls. In contrast, the levels of glutathione total (p<0.05) and glutathione reduced (p<0.05) were significantly elevated in head and neck squamous cell carcinoma tissues compared to the adjacent cancer-free control tissues. In addition to this, pearson correlation performed to correlate different tissue glutathione levels (GSH) with clinical/pathological parameters demonstrated a significant negative correlation between pT-stage and GSH level ($r=-0.263^{**}$; p<0.01), C-stage and GSH level ($r=-0.335^{**}$; p<0.01), grade and GSH ($r=-0.329^{**}$; p<0.01) and grade versus redox index ($r=-0.213^{**}$; p<0.01) in HNSCC tissues. Our study suggests that dysregulation of glutathione levels in head and neck cancer has the potential to predict metastasis, and may serve as a prognostic marker.

• 대한수의학회지
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• 제46권1호
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• pp.35-41
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• 2006

Vitellogenin (Vtg), a phospholipoglycoprotein precursor of egg yolk is synthesized and secreted from the liver in response to estrogens in female fish. Vtg is normally undetectable in the blood of male fish, but can be induced by exposure to chemicals possessing estrogenic activity. Thus, the presence of Vtg in blood of male fish can serve as a useful biomarker for assessing previous exposure to estrogenic compounds. In the present study, Vtg was abnormally expressed in Rhynchocypris oxycephalus using estradiol benzoate ($E_2$). As the result, it was found that the level of Vtg in blood from R. oxycephalus was increased by treated quantity of $E_2$ with dose-effect manner. Monoclonal antibodies were generated against Vtg of R. oxycephalus. The hybridoma were screened with an enzyme immunoassay for the production of specific anti-Vtg antibodies. Five positive cell lines with a high specificity were selected. Monoclonal antibodies against vtg of R. oxycephalus that was developed in this study, may be a useful bio-indicator for the detection of estrogenic contamination in the aquatic ecosystem.

• STI Policy Review
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• 제7권2호
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• pp.106-131
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• 2016

The general purpose of this paper is to identify opportunities for and to measure existing collaboration on research and development between institutions from the countries of Asia and Latin America in FEALAC's framework, in the fields of biotechnology and nanotechnology and their convergence. The methodological approach includes scientific and technological surveillance and research seeking to identify both the R&D and innovation capacities of the countries as well as the degree of international cooperation between countries of the two regions; case studies and a study of the governance framework of international collaboration in R&D about issues considered global challenges. The study has three main findings. First, nanotechnology, biotechnology and their convergence contribute to solving the problem of contamination by heavy metals affecting most of the countries that are part of FEALAC and to address problems arising from the accelerated rate of energy consumption, which also contributes to environmental damage. In this scenario, important business opportunities arise from the adaptation and development of bio-refinery technologies. Second, the scientific relationship between FEALAC countries, mainly between Asian and Latin American countries, is weak as can be seen in research for articles and patents. But there is plenty of room and potential for improvement. Third, current and upcoming joint R&D programs and projects should be linked both to existing governance structures and to new ones that serve as experiments of STI public policy regarding innovative management of intellectual property and capacity building. Practical implications are included in lessons learned and a set of recommendations involving a couple of proposals. One proposal calls for research and innovation in promising fields for international cooperation. Another proposal creates mechanisms in the governance framework for sharing knowledge, capacity building, and funding.

• 한국물환경학회지
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• 제38권2호
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• pp.63-71
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• 2022

This study aimed to maximize the potential of fluorescence 3D excitation-emission matrix (EEM) for predicting the trihalomethane formation potential (THMFP) of DOM with various sources. Fluorescence spectroscopy is a useful tool for characterizing dissolved organic matter (DOM). In this study, differential spectroscopy was applied to EEM for the prediction of THMFP, in which the difference between the EEM before and after chlorination was taken into account to obtain the differential EEM (DEEM). For characterization of the original EEM or the DEEM, the maximum intensities of several different fluorescence regions in EEM, fluorescence EEM regional integration (FRI), and humification index (HIX) were calculated and used for the surrogates for THMFP prediction. After chlorination, the fluorescence intensity decreased by 77% to 93%. In leaf-derived and effluent DOM, there was a significant decrease in the protein-like peak, while a more pronounced decrease was observed in the humic-like peak of river DOM. In general, leaf-derived and effluent DOM exhibited a relatively lower THMFP than the river DOM. Our results were consistent with the high correlations between humic-like fluorescence and THMFP previously reported. In this study, HIX (r= 0.815, p<0.001), FRI region V (r=0.727, p<0.001), humic-like peak (r= 0.827, p<0.001) from DEEM presented very high correlations with THMF P. When the humic-like peak intensity was converted to a logarithmic scale, a higher correlation was obtained (r= 0.928, p<0.001). This finding suggests that the humic-like peak in DEEM can serve as a universal predictor for THM formation of DOM with various origins.

• 한국환경성돌연변이발암원학회지
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• 제23권2호
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• pp.56-62
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• 2003

The liver progenitor cells could form a potential target cell population fore both tumor-initiating and -promoting chemicals. Induction of drug-metabolizing and antioxidant enzymes, including AhR-dependent CYP1A1, NQO-1 and AKR1C9, was detected in the rat liver epithelial WB-F344 "stem-like" cells. Additionally, WB-F344 cells express a functional, wild-type form of p53 protein, a biomarker of genotoxic events, and connexin 43, a basic structural unit of gap junctions forming an important type of intercellular communication. In this cellular model, two complementary assays have been established for detection of the modes of action associated with tumor promotion: inhibition of gap junctional intercellular communication (GJIC) and proliferative activity in confluent cells. We found that the PAHs and PCBs, which are AhR agonists, released WB-F344 cells from contact inhibition, increasing both DNA synthesis and cell numbers. Genotoxic effects of some PAHs that lead to apoptosis and cell cycle delay might interfere with the proliferative activity of PAHs. Contrary to that, the nongenotoxic low-molecular-weight PAHs and non-dioxin-like PCB congeners, abundant in the environment, did not significantly affect cell cycle and cell proliferation; however both groups of compounds inhibited GJIC in WB-F344 cells. The release from contact inhibiton by a mechanism that possibly involves the AhR activation, inhibition of GJIC and genotoxic events induced by environmental contaminants are three important modes of action that could play an important role in carcinogenic effects of toxic compounds. The relative potencies to inhibit GJIC, to induce AhR-mediated activity, and to release cells from contact inhibition were determined for a large series of PAHs and PCBs and their metabolites. In vitro bioassays based on detection of events on cellular level (deregulation of GJIC and/or proliferation) or determination of receptor-mediated activities in both ?$stem-like^{\circ}{\times}$ and hepatocyte-like liver cellular models are valuable tools for detection of modes of action of polyaromatic hydrocarbons. They may serve, together with concentration data, as a first step in their risk assessment.

• Clinical Nutrition Research
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• 제11권1호
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• pp.20-31
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• 2022

Through a survey on dietary intake of children and adolescents with brain lesions, the present study aimed to analyze the current status of nutrient intake and examine the effect of high-protein nutrient drink on their nutritional and muscle statuses. The study participants were 90 juvenile participants aged 8-19 years, with brain lesions. The participants were provided with a protein nutrient drink for 12 weeks and a questionnaire survey on dietary intake was performed to analyze the level of nutrient intake before and after ingestion. The physical measurements were taken to determine the improvements in nutrient and muscle statuses. The results showed that, before the intake of protein nutrient drink as a supplement, the participants exhibited lower height, weight, and body mass index than those of the standard levels of healthy individuals, and the level of nutrient intake through diet was lower than those of the required and recommended levels of nutrient intake for Koreans. Conversely, after the intake of protein nutrient drink for 12 weeks, the level of nutrient intake and physical statuses such as weight showed significant improvements. In addition, the muscle status had undergone approximately 10% of change during the intervention with no significant difference. Thus, to ensure an adequate level of nutrient supply to children and adolescents with brain lesions, there is an urgent need to develop a guideline of nutrient intake. The findings in this study are expected to serve as the basic data for such guidelines.

• 한국해양환경ㆍ에너지학회지
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• 제19권2호
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• pp.165-171
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• 2016

본 연구는 해양수산 연구개발사업의 과제별 특성, 연구성과 및 사업화 실적 현황을 파악하여 해양수산 분야 기술사업화 관련 정책 결정에 시사점을 제공하고자 하는 목적을 가지고 있다. 이를 위해 2010년부터 2014년까지의 해양R&D 144개 과제와 수산R&D 68개 과제, 총 212개 과제를 대상으로 정부지원 연구비규모별 현황, 연구수행주체별 구분, 과제 진행 기간에 따른 현황을 파악하였다. 사업화 현황과 성과는 논문, 특허, 기술료 및 기술이전, 공공서비스 개선으로 나눠 분석하였다. 분석을 통해 본 연구에서는 그간 정부가 추진해 온 연구주체 다변화 도모, 연구인프라 공동활용 정책의 지속적 추진이 필요하며, 연구수행 주체의 특성에 맞는 맞춤형 연구관리 시스템의 도입, 시장 수요를 반영한 자유공모 과제 비중 확대 그리고 실용화 목적의 성과창출 위한 투자 추진의 필요성을 정책적 시사점으로 제안하였다.

• Molecules and Cells
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• 제41권9호
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• pp.830-841
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• 2018

Recent studies have indicated that microRNAs (miRNAs) play an important role in hepatocellular carcinoma (HCC) progression. In this study, we showed that miR-766-3p was decreased in approximately 72% of HCC tissues and cell lines, and its low expression level was significantly correlated with tumour size, TNM stage, metastasis, and poor prognosis in HCC. Ectopic miR-766-3p expression inhibited HCC cell proliferation, colony formation, migration and invasion. In addition, we showed that miR-766-3p repressed Wnt3a expression. A luciferase reporter assay revealed that Wnt3a was a direct target of miR-766-3p, and an inverse correlation between miR-766-3p and Wnt3a expression was observed. Moreover, Wnt3a up-regulation reversed the effects of miR766-3p on HCC progression. In addition, our study showed that miR-766-3p up-regulation decreased the nuclear ${\beta}-catenin$ level and expression of Wnt targets (TCF1 and Survivin) and reduced the level of MAP protein regulator of cytokinesis 1 (PRC1). However, these effects of miR-766-3p were reversed by Wnt3a up-regulation. In addition, PRC1 upregulation increased the nuclear ${\beta}-catenin$ level and protein expression of TCF1 and Survivin. iCRT3, which disrupts the ${\beta}-catenin-TCF4$ interaction, repressed the TCF1, Survivin and PRC1 protein levels. Taken together, our results suggest that miR-766-3p down-regulation promotes HCC cell progression, probably by targeting the Wnt3a/PRC1 pathway, and miR-766-3p may serve as a potential therapeutic target in HCC.

• 한국작물학회지
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• 제51권1호
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• pp.81-88
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• 2006

The 117 soybean cultivars were collected from nine provinces in Korea, and various seed quality traits along with isoflavone contents were evaluated to elucidate their relationship. The 100-seed weight of the black soybean (31.2 g) was significantly higher (p<0.05) than yellow soybeans (28.6 g). The composition of genistein, daidzein, and glycitein accounted for 75.8, 22.8, and 1.4 % of total isoflavone in yellow soybean cultivars, while their compositions in black soybeans were 58.5, 39.7, and 1.8%, respectively. The mean contents of total isoflavone in yellow and black soybean were $l,561.6{\mu}g\;g^{-1}\;and\;l,018.3{\mu}g\;g^{-1}$. The isofalvone content showed significant variation among cultivars when classified by the seed size. In the yellow soybeans, total isoflavone content was higher in small size soybean cultivars $(1,776.0{\mu}g\;g^{-1})$ and medium size soybean cultivars $(1,714.3{\mu}g\;g^{-1})$ compared to large size ones $(1,518.5{\mu}g\;g^{-1})$. Genistein content was proved as the major factor determining the relationship between isoflavone content and 100-seed weights (r =-0.206*). Daidzein and glycitein, however, showed no significant relationship with the 100-seed weights. Isoflavone content was not significantly correlated with color parameters L (lightness) and a (redness) values, but color parameter b (yellowness) was positively correlated with glycitein (r=0.264*) in the yellow soybeans, while its negative correlation between daidzein (r=-0.245*) and total isoflavone (r=-0.256*) were observed in black soybeans. However, these findings suggested that the seed color value may not serve as an effective parameter for estimating the isoflavone intensity of the soybeans. Variation of protein and lipid contents between yellow soybeans (n=58) and black soybeans (n=59) was relatively stable, however, protein and lipid contents have no significant relationship with isoflavone content.