• Title/Summary/Keyword: R&D Program

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NADPH Oxidases Are Required for Appressorium-Mediated Penetration in Colletotrichum scovillei-Pepper Fruit Pathosystem

  • Fu, Teng;Lee, Noh-Hyun;Shin, Jong-Hwan;Kim, Kyoung Su
    • The Plant Pathology Journal
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    • v.38 no.4
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    • pp.345-354
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    • 2022
  • NADPH oxidase (Nox) complexes are known to play essential roles in differentiation and proliferation of many filamentous fungi. However, the functions of Noxs have not been elucidated in Colletotrichum species. Therefore, we set out to characterize the roles of Nox enzymes and their regulators in Colletotrichum scovillei, which causes serious anthracnose disease on pepper fruits in temperate and subtropical and temperate region. In this study, we generated targeted deletion mutants for CsNox1, CsNox2, CsNoxR, and CsNoxD via homologous recombination. All deletion mutants were normal in mycelial growth, conidiation, conidial germination, and appressorium formation, suggesting that CsNox1, CsNox2, CsNoxR, and CsNoxD are not involved in those developmental processes. Notably, conidia of 𝜟Csnox2 and 𝜟Csnoxr, other than 𝜟Csnox1 and 𝜟Csnoxd, failed to cause anthracnose on intact pepper fruits. However, they still caused normal disease on wounded pepper fruits, suggesting that Csnox2 and CsnoxR are essential for penetration-related morphogenesis in C. scovillei. Further observation proved that 𝜟Csnox2 and 𝜟Csnoxr were unable to form penetration peg, while they fully developed appressoria, revealing that defect of anthracnose development by 𝜟Csnox2 and 𝜟Csnoxr resulted from failure in penetration peg formation. Our results suggest that CsNox2 and CsNoxR are critical for appressorium-mediated penetration in C. scovillei-pepper fruit pathosystem, which provides insight into understanding roles of Nox genes in anthracnose disease development.

Missionary Public Health Nursing of Korea during Japanese Colonial Period (일제시대 선교회의 보건간호사업에 대한 역사적 연구)

  • Yi, Ggod-Me;Kim, Hwa-Joong
    • Research in Community and Public Health Nursing
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    • v.10 no.2
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    • pp.455-466
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    • 1999
  • Western missionary nurses practiced in Korea from 1891. and the first trial to begin missionary public health nursing service in 1909 could not put into practice for short of nursing staff and budget. The main focus of missionary medical practice was not in public health program but in the management of missionary hospitals. A few of missionary western R.N. tried district nursing in 1910s. but their activities were personal and focused on the rescue of poor and sick patients. In 1917 the North American Methodist Church dispatched R.N. Elizabeth S. Roberts to begin district nursing in Korea. Roberts began maternal and child district nursing service. Her service was focused on teaching the method of bringing up children. bathing service, and home visiting for delivery. She could not but stop district-nursing service in 1918 to serve for a hospital in Siberia. The North American Methodist Church dispatched a few of R.N. to Korea in early 1920s and the missionary public health nursing of Korea could be activated. R.N. E. T. Rosenberger began public health nursing program in Seoul with Korean graduate nurse, Shin-gwang Han, and missionary M.D. Hall. Their public health nursing program was focused on maternal and childcare. They did home visiting in the morning, and served at a well baby clinic in the afternoon. The first baby competition began in 1925. and contributed to the teaching the method of bringing up children. They expanded public health nursing activity to school health nursing and milk station. Their public health nursing program was such a success that In 1929 Severance hospital. Eastgate Hospital. Taehwa Social Evangelistic center organized Seoul Child Health Union. Maren P. Bording, another missionary R.N. and midwife dispatched by the North American Methodist Church began public health nursing program at Kongjoo in 1924. Her program was focused on the maternal and childcare and close to that of Seoul. She started the first milk station in Korea in 1926. As she was a midwife and could get M. D. license in Korea, her program was more focused on maternal care than that of Seoul. The first day nursery school in Korea and the first graduate course for public health nursing in Korea began at Kongjoo in 1930. As the city of Choongcheongnam Province moved from Kongjoo to Daejeon in 1932, missionary public health nursing service in Kongjoo extended to Daejeon. There were lots of public health nursing program in Korea in 1920s and 1930s by missionary western nurses and Korean nurses. There were 13 missionary public health-nursing center in Korea in 1932. But in the late 1930s. Japan extended colonial war and drove out western missionaries. The missionary service in Korea was daunted. and the missionary public health nursing service could not but shrink.

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Development of Vibration Prediction Program of Gas Pipeline by Construction Vibration (건설진동에 의한 가스배관의 진동예측 프로그램 개발)

  • Jeong S. Y.;Hong S. K.;Kim J. H.;Koh J. P.
    • Journal of the Korean Institute of Gas
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    • v.5 no.2 s.14
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    • pp.30-35
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    • 2001
  • Presently, working gas pipelines are being subjected to the influence of construction vibration. Especially on subway and road construction, gas pipelines are being influenced to construction vibration caused by use of construction equipment, passage of a large-sized vehicle and blasting. Buried gas pipelines are subjected to the influence of vibration caused by blast in the vicinity of pipeline, exposed gas pipelines are subjected to the influence of vehicle vibration. Therefore, in the study, it is developed to vibration prediction program of gas pipeline by analyzing measured construction vibration. This program is able to predict vibration of gas pipeline according to field conditions by using the results of structural finite element analysis and empirical equation by reliability analysis. And, this program contains the database of construction vibration. Additionally, this program is able to compute estimated blast vibration equation using measured blast vibration data in the field and to form graph of allowable charging gunpowder per delayed-action with the change of blast velocity. Therefore, field workers are able to predict construction vibration around gas pipeline and estimate safety of gas pipeline.

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Phylogenetic Analysis of Dendropanax morbifera Using Nuclear Ribosomal DNA Internal Transcribed Spacer (ITS) Region Sequences (Internal transcribed spacer (ITS) region의 염기서열 분석에 의한 보길도산 황칠나무의 분자 계통학적 연구)

  • Shin, Yong Kook
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1341-1344
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    • 2016
  • Dendropanax morbifera is an endemic tree species of Korea, it is restricted to the southern parts of Korea. The internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) for Dendropanax morbifera grown at Bogil-do, Korea was determined. We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. The determined sequences consisted of 689 residues. ITS1 was 222 bp long while ITS2 was 233 bp long. The 5.8S rDNA was 160 bp long. The ITS region sequences of the Dendropanax species included in this study were obtained from GenBank. Oreopanax polycephalus was used as the outgroup. A pairwise alignment was calculated using the Clustal X program. A phylogenetic tree was constructed by the neighbor-joining method using the Tree view program. Sequence similarities among species including D. morbifera Bogil-do isolate showed the range 92.6 to 99.7% in sequence-based phylogenetic analysis using total 615 base pairs of ITS1, 5.8S rDNA and ITS2. D. morbifera Bogil-do isolate exhibited the highest degree of relatedness to D. chevalieri, sharing 99.7% ITS region similarity. D. morbifera Bogil-do isolate also showed to D. trifidus, sharing 99.4% ITS region similarity.

Consolidation Analysis of Dredged Fill Ground Installed with Horizontal Drains (I) - Program Development and Verification - (수평배수재가 포설된 준설매립지반의 압밀해석(I) - 프로그램 개발 및 검증 -)

  • Park Chung-Yong;Jang Yeon-Soo;Park Chung-Soon
    • Journal of the Korean Geotechnical Society
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    • v.21 no.10
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    • pp.27-39
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    • 2005
  • A finite difference program with 3-D governing equation expanded from 1-D self-weight consolidation is developed to analyze the consolidation behavior of surface dredged soil with horizontal drains. Various boundary conditions with horizontal drains and seepage pressure of pore water infiltrated to the drains are considered in the program. A laboratory soil chamber experiment for the consolidation of dredged soil is performed to validate the program and the measured settlement-time result is compared with the one predicted by the program. The influence of design conditions of horizontal drains such as horizontal installation spacing, installation depth and number of drain layers, on the consolidation is analyzed.

The Study on the Exterior Design Decision Process of the Maneuver Weapon System Development (기동무기체계 개발에서 외형 디자인 결정 프로세스에 관한 연구)

  • Youn, Byungjo;Lee, Ho
    • Journal of the Korean Society of Systems Engineering
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    • v.13 no.2
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    • pp.1-8
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    • 2017
  • The Core in Weapon system R&D is to realize the user required capabilities in limited schedule and cost. Therefore, the design of weapon system focuses to functional design for realization of requirement capabilities. However, the weapon system should not only basically bring the confidence to friendly forces bring fear to enemy forces to maximize the operational effectiveness of weapon systems and we consider the exterior design as the important factor to compete with foreign systems in international defense market besides capabilities and cost. For that reason, we made study case about the visual design decision process applied to the LVT(light tactical vehicle) project which was recent successful deployment among the many R&D projects of auto industry. Based on this case, this study suggests the necessity of the exterior design decision process the maneuver weapon system is developed.

A Planning Study on the Fusion Technology Project in National R&D Program(II (국가연구개발사업의 융합기술과제 기획연구(Ⅱ))

  • Kim, Hyun-Cheol;Son, Chung-Geun;Na, Kyoung-Hoan
    • Proceedings of the KSME Conference
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    • 2007.05a
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    • pp.490-495
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    • 2007
  • 지식기반경제시대는 과학기술 혁신과 창조적 능력이 국가성장을 좌우함. 세계적으로 이종기술간의 융합을 통한 신시장 창출이라는 뉴 패러다임이 등장하고 있으며, 우리나라도 현재 처한 넛크랙트 위기를 돌파할 수 있는 창조형 연구 전략의 수립이 절실함. 원천융합기술은 기술의 성격상 와해적이며 블루오션 신시장 창출가능이 높으며, 따라서, 향후 미래 신산업 및 신시장 창출을 위해서는 기초원천 및 미래태동 기술에 부합하는 사업발굴이 필요한 상황임. 이에 본 기획연구에서는 미래유망 파이오니어사업의 개념, 주요특징, 파이오니어사업에서 추구하는 원천융합기술의 제반 환경, 사업성 분석 및 과학기술적 기대성과와 경제사회적 파급효과 등을 조사/분석하고자함

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A Study on Selecting the Key Research Areas in Nano-technology Field in Korea: An Application of Technology Cluster Analysis in National R&D Program (한국의 나노기술 분야에서 핵심 연구영역 도출에 관한 연구 -국가 연구개발사업 수준에서 기술군집분석의 적용-)

  • 이용길;이세준;이재영
    • Journal of Korea Technology Innovation Society
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    • v.6 no.2
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    • pp.175-190
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    • 2003
  • This paper deals with the methods for selecting the key research areas, which fit for the large, multi-disciplinary, and long-term programs by making use of Technology Cluster Analysis. This method is applied to mano-technology field at the level of national R&D program. 56 nano-technologies are analyzed and grouped into three main clusters based on the survey data from 180 experts. Three main clusters are \circled1 naro-materials related cluster, \circled2 naro-device related cluster, and \circled3 naro-bio related cluster. These three clusters are coincided with the focused areas of nano-technology in Korea. Each cluster is analyzed in view of its competence position.

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Technology Development of Smart UAV and Application of CFD (스마트무인기 기술개발동향 및 전산유체공학의 적용)

  • Lim Cheol-Ho;Hwang Soo-Jung;Choi Seong-Wook
    • 한국전산유체공학회:학술대회논문집
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    • 2002.10a
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    • pp.8-16
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    • 2002
  • Now, the era of information, the rapid acquisition of vast and various information acts as a key role. for the national competitiveness. The smart UAV is brought to the fore as a new future technology which will be able to satisfy the information needs in broad and stereoscopic manner. The smart UAV, with VTOL capability, higher performance, and reliability over the existing technology thresholds, will lead the aerospace technology in the future. In this paper, the technology concept of smart UAV, the necessity of development, and the trend of worldwide R&D are introduced and analyzed. In addition, for the Smart UAV Development Program which is launched by the 21c Frontier R&D program this year, the role and application area of the CFD will be discussed.

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Development of the CFD Program for the Cold Gas Flow Analysis in a High Voltage Circuit Breaker Using the CFD-CAD Integration (CFD-CAD 통합해석을 위한 초고압 차단기 내부의 냉가스 유동해석 프로그램 개발)

  • Lee, J.C.;Oh, I.S.
    • Proceedings of the KIEE Conference
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    • 2001.10a
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    • pp.30-32
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    • 2001
  • There are many difficult problems in analyzing the flow characteristics in a high voltage circuit breaker such as shock wave and complex geometries, which may be either static or in relative motion. Although a variety of mesh generation techniques are now available, the generation of meshes around complicated, multi-component geometries like a gas circuit breaker is still a tedious and difficult task for the computational fluid dynamics. This paper presents the CFD program for analyzing the compressible flow fields in a high voltage gas circuit breaker using the Cartesian cut-cell method based on the CFD-CAD integration, which can achieve the accurate representation of the geometry designed by a CAD tools. This technique is frequently satisfied, and it will be almost universally so in the future, as the CFD-CAD traffic increase.

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