• Title/Summary/Keyword: R&D Department

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MiR-30a-5p and miR-153-3p regulate LPS-induced neuroinflammatory response and neuronal apoptosis by targeting NeuroD1

  • Choi, Hye-Rim;Ha, Ji Sun;Kim, Eun-A;Cho, Sung-Woo;Yang, Seung-Ju
    • BMB Reports
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    • v.55 no.9
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    • pp.447-452
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    • 2022

  • Neurogenic differentiation 1 (NeuroD1) is an essential transcription factor for neuronal differentiation, maturation, and survival, and is associated with inflammation in lipopolysaccharide (LPS)-induced glial cells; however, the concrete mechanisms are still ambiguous. Therefore, we investigated whether NeuroD1-targeting miRNAs affect inflammation and neuronal apoptosis, as well as the underlying mechanism. First, we confirmed that miR-30a-5p and miR-153-3p, which target NeuroD1, reduced NeuroD1 expression in microglia and astrocytes. In LPS-induced microglia, miR-30a-5p and miR-153-3p suppressed pro-inflammatory cytokines, reactive oxygen species, the phosphorylation of c-Jun N-terminal kinase, extracellular-signal-regulated kinase (ERK), and p38, and the expression of cyclooxygenase and inducible nitric oxide synthase (iNOS) via the NF-κB pathway. Moreover, miR-30a-5p and miR-153-3p inhibited the expression of NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasomes, NLRP3, cleaved caspase-1, and IL-1β, which are involved in the innate immune response. In LPS-induced astrocytes, miR-30a-5p and miR-153-3p reduced ERK phosphorylation and iNOS expression via the STAT-3 pathway. Notably, miR-30a-5p exerted greater anti-inflammatory effects than miR-153-3p. Together, these results indicate that miR-30a-5p and miR-153-3p inhibit MAPK/NF-κB pathway in microglia as well as ERK/STAT-3 pathway in astrocytes to reduce LPS-induced neuronal apoptosis. This study highlights the importance of NeuroD1 in microglia and astrocytes neuroinflammation and suggests that it can be regulated by miR-30a-5p and miR-153-3p.

  • https://doi.org/10.5483/BMBRep.2022.55.9.061 인용 PDF KSCI

An Analysis on the Economic Validity of the Establishment of R&D Supreme Academy (R&D 인력 재교육 전문기관의 경제적 타당성 분석)

  • Lim, Chang-Joo;Kim, Sang-Yung;Baek, Nak-Ki;Oh, Sea-Hong;Seo, Jong-Hyen
    • Journal of the Korea Safety Management & Science
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    • v.11 no.3
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    • pp.181-188
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    • 2009

  • This paper presents the economic validity with the cost/benefit analysis on establishing R&D Supreme Academy. To evaluate the profitability of R&D Supreme Academy, willingness to pay (WTP) is measured by contingent valuation method (CVM) and then the net present value (NPV), internal rate of return (IRR) and benefit/cost ratio(BCR) are calculated. The results of economic evaluation verified that the establishment of R&D Supreme Academy is timely and effective in an economic perspective.

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Preparation and Characterization of Cobalt(III) Complexes with N,N,N',N'-Tetrakis(2-aminoethyl)-1,2-ethanediamines Ligand (,N,N',N'-Tetrakis(2-aminoethyl)-1,2-ethanediamine형 리간드를 포함하는 코발트 (III) 착물의 합성과 특성)

  • Myung-ki Doh;Byeoung-Su Choi;Chang-Rok An;Junnosuke Fujita
    • Journal of the Korean Chemical Society
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    • v.26 no.5
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    • pp.310-319
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    • 1982

  • Cobalt(III) complexes with sexidentate ligands, N,N,N',N'-tetrakis(2-amino-ethyl)-1,2-ethanediamine (ten), -1,3-propanediamine (ttn), -1,4-butanediamine (ttmd), -(R,R)-and -(R,S)-2,4-pentanediamine (tptn) were prepared, and the characterization of d-d absorption band on the variation of chelate ring size and conformation of these complexes were studied by means of electronic spectra. The first d-d absorption bands of $[Co(L)]^{3+}$ complexes are shifted to smaller wave numbers in the order. ttn > (R,R)-tptn > ten > ttmd${\simeq}$(R,S)-tptn for (L). The UV, $^{13}C$ NMR, and Circular Dichroism studies indicate that the R,S-tptn ligand of $[Co(R,S-tptn)]^{3+}$ complex coodinates to cobalt(Ⅲ) ion as a sexidentate with one methyl group in axial position.

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