• 생명과학회지
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• 제13권4호
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• pp.375-382
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• 2003

본 연구는 콩 cyst 선충 race 14에 대한 저항성 QTLs 구명을 목적으로 한 바 결과를 요약하면 다음과 같다. 1. 회귀분석 결과 30개의 marker들(29 RAPD, 1 RFLP)에서 cyst 선충 race 14의 저항성에 대한 유의성이 인정되었다. 2. MAPMAKER/QTL 분석 결과 2개의 QTL들이 구명되었는데, 이 QTL들은 2개의 linkage groups (LGC-7와 LGC-9)에 위치하였으며, 모두 우성유전 양상을 나타내었다. 3. 다중회귀분석 결과 2개의 marker들($B15^2$와 $H06^1$)로 구성된 조합에서 가장 높은 표현적 변이의 값(22.9%)을 나타내었다. 콩 cyst 선충 rare 14에 대한 표현적 변이를 충분히 설명하기 위해서는 지속적인 QTL 구명 연구가 요구된다.

• 한국축산식품학회지
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• 제31권3호
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• pp.325-338
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• 2011

Investigations into fatty acid composition in meats are becoming more important due to consumer demand for high quality healthy food. Marker-assisted selection has been applied to livestock to improve meat quality by directly selecting animals for favorable alleles that affect economic traits. Quantitative trait loci affecting fatty acid composition in cattle and pigs were investigated, and five candidate genes (ACACA, FASN, SCD, FABPs, and SREBP-1) were significantly associated with fatty acid composition. The information presented here should provide valuable guidelines to detect causative mutations affecting fatty acid composition in cattle and pigs.

• Asian-Australasian Journal of Animal Sciences
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• 제14권4호
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• pp.587-596
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• 2001

In the last decade, rapid developments in molecular biotechnology and of genomic tools have enabled the creation of dense linkage maps across whole genomes of human, plant and animals. Successful development and implementation of interval mapping methodologies have allowed detection of the quantitative trait loci (QTL) responsible for economically important traits in experimental and commercial livestock populations. The candidate gene approach can be used in any general population with the availability of a large resource of candidate genes from the human or rodent genomes using comparative maps, and the validated candidate genes can be directly applied to commercial breeds. For the QTL detected from primary genome scans, two incipient fine mapping approaches are applied by generating new recombinants over several generations or utilizing historical recombinants with identity-by-descent (IBD) and linkage disequilibrium (LD) mapping. The high resolution definition of QTL position from fine mapping will allow the more efficient implementation of breeding programs such as marker-assisted selection (MAS) or marker-assisted introgression (MAI), and will provide a route toward cloning the QTL.

• Genomics & Informatics
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• 제14권4호
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• pp.211-215
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• 2016

The alteration of alternative splicing patterns has an effect on the quantification of functional proteins, leading to phenotype variation. The splicing quantitative trait locus (sQTL) is one of the main genetic elements affecting splicing patterns. Here, we report the results of genome-wide sQTLs across 141 strains of Arabidopsis thaliana with publicly available next generation sequencing datasets. As a result, we found 1,694 candidate sQTLs in Arabidopsis thaliana at a false discovery rate of 0.01. Furthermore, among the candidate sQTLs, we found 25 sQTLs that overlapped with the list of previously examined trait-associated single nucleotide polymorphisms (SNPs). In summary, this sQTL analysis provides new insight into genetic elements affecting alternative splicing patterns in Arabidopsis thaliana and the mechanism of previously reported trait-associated SNPs.

• Journal of Plant Biotechnology
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• 제44권4호
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• pp.349-355
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• 2017

The amino acid composition of rice is a major concern of rice breeders because amino acids are among the most important nutrient components in rice. In this study, a genetic map was constructed with a population of 134 recombinant inbred lines (RILs) from a cross between Dasanbyeo (Tongil-type indica) and TR22183 (temperate japonica), as a means to detect the main and epistatic effect quantitative trait loci (QTLs) for the amino acid content (AAC). Using a linkage map which covered a total of 1458 cM based on 239 molecular marker loci, a total of six main-effect QTLs (M-QTLs) was identified for the content of six amino acids that were mapped onto chromosome 3. For all the M-QTLs, the TR22183 allele increased the trait values. The QTL cluster (flanked by id3015453 and id3016090) on chromosome 3 was associated with the content of five amino acids. The phenotypic variation, explained by the individual QTLs located in this cluster, ranged from 10.2 to 12.4%. In addition, 26 epistatic QTLs (Ep-QTLs) were detected and the 25 loci involved in this interaction were distributed on all nine chromosomes. Both the M-QTLs and Ep-QTLs detected in this study will be useful in breeding programs which target the development of rice with improved amino acid composition.

• 영어어문교육
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• 제15권3호
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• pp.249-282
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• 2009

This study reports on the use of the trait and state cognitive/metacognitive strategy use and the difference of the trait/state strategy use according to students' proficiency level. First of all, for checking up the trait strategy use, 119 first-grade male students in a high school completed the questionnaire on strategies which they thought they used during a reading test. Secondly, to find out their state strategy use, students took a fifteen-item reading comprehension test, followed by filling out questionnaires on cognitive and metacognitive strategies used in the test. This study employed quantitative data analysis. The results suggested that (1) the cognitive and metacognitive strategy use in the trait and state conditions are used the most by the high proficiency group and they are correlated respectively; (2) these strategies are used with statistically significant difference according to students' proficiency level, especially to the lower level students. The highly successful group uses the cognitive and metacognitive strategy in the actual test situation more than the lower proficient group; there is no difference in trait and state strategy use.

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1505-1510
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• 2001

A method for mapping quantitative trait loci (QTL) was introduced incorporating the information of mixed progeny from complex pedigrees. The method consisted of two steps based on single marker analysis. The first step was to examine the marker-trait association with a mixed model considering common environmental effect and reversed QTL-marker linkage phase. The second step was to estimate QTL effects by a weighted least square analysis. A simulation study indicated that the method incorporating mixed progeny from multiple generations improved the accuracy of QTL detection. The influence of within-genotype variance and recombination rate on QTL analysis was further examined. Detecting a QTL with a large within-genotype variance was more difficult than with a small within-genotype variance. Most of the significant marker-QTL association was detectable when the recombination rate was less than 15%.

• Asian-Australasian Journal of Animal Sciences
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• 제24권11호
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• pp.1525-1528
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• 2011

The purpose of this study was to detect SNPs that were responsible for a carcass trait in Hanwoo populations. A non-parametric model applying a restricted partition method (RPM) was used, which exploited a partitioning algorithm considering statistical criteria for multiple comparison testing. Phenotypic and genotypic data were obtained from the Hanwoo Improvement Center, National Agricultural Cooperation Federation, Korea, in which the pedigree structure comprised 229 steers from 16 paternal half-sib proven sires that were born in Namwon or Daegwanryong livestock testing station between spring of 2002 and fall of 2003. A carcass trait, longissimus dorsi muscle area for each steer was measured after slaughter at approximately 722 days. Three SNPs (19_1, 18_4 and 28_2) near the microsatellite marker ILSTS035 on BTA6, around which the quantitative trait loci (QTL) for meat quality were previously detected, were used in this study. The RPM analyses resulted in two significant interaction effects between SNPs (19_1 and 18_4) and (19_1 and 28_2) at ${\alpha}$ = 0.05 level. However, under a general linear (parametric) model no interaction effect between any pair of the three SNPs was detected, while only one main effect for SNP19_1 was found for the trait. Also, under another non-parametric model using a multifactor dimensionality reduction (MDR) method, only one interaction effect of the two SNPs (19_1 and 28_2) explained the trait significantly better than the parametric model with the main effect of SNP19_1. Our results suggest that RPM is a good alternative to model choices that can find associations of the interaction effects of multiple SNPs for quantitative traits in livestock species.

• 한국식품영양학회지
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• 제18권3호
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• pp.229-240
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• 2005

This quantitative study was investigated to examine the relationship between severity of state-trait anxiety and disordered eating patterns in some university students. This study used a cross-sectional study design. Total 347 students participated in this study (88 male and 259 female) among three universities. The assessment of eating disorder was conducted by Eating Attitudes Test (EAT-26), a score of >or=20 identified individuals likely to have an eating disorder, including anorexia nervosa and bulimia nervosa. Scores of healthy dietary behaviors were obtained by self-assessment instrument on healthy diet scale(20-item questionnaire), and severity of state-trait anxiety was calculated by state-trait anxiety inventory(Total 40- item questionnaire). In groups for each state anxiety and trait anxiety, there were divided between 50 percentile point of cumulatived scores of state anxiety and trait anxiety in all subjects. Linear regression analysis showed overall significant difference between dietary patterns(anorexia nervosa and healthy dietary behaviors) and severity of state-trait anxiety in all sex. Our results indicated that severity of state-trait anxiety may marked eating disorder symptomatology on dimensions of eating disorder prevention.

• Asian-Australasian Journal of Animal Sciences
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• 제24권10호
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• pp.1329-1334
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• 2011

The Hinai-dori is a breed of chicken native to Akita Prefecture, Japan. An $F_2$ resource population produced by crossing low- and high-growth lines of the Hinai-dori breed was analyzed to detect quantitative trait loci (QTL) for growth traits. Highly significant QTLs for body weight at 10 and 14 weeks of age and average daily gain between 4 and 10 weeks and between 10 and 14 weeks of age were accordingly mapped in a common region between ADL0198 and ABR0287 on chromosome 1 and between MCW0240 and ABR0622 on chromosome 4, respectively. A significant QTL for body weight at 4 weeks of age and a significant QTL for average daily gain between 0 and 4 weeks of age were mapped for the first time to the same region flanking ABR0204 and ABR0284 on chromosome 1. These QTLs are good candidates for application in the development of marker-assisted selection strategies for increasing growth efficiencies in the Hinai-dori breed and native breeds of chickens in Asia.