• Journal of the Society of Naval Architects of Korea
• /
• v.36 no.1
• /
• pp.82-89
• /
• 1999

A designer must make a lot of decisions in a design process. The decisions may be classified into selection decisions and compromise decisions. As the results of two decisions depends on the designer's intention it is necessary that the designer's intention should be reflected in the design systematically and precisely. As the AHP(Analytic Hierarchy Process) technique analyzes and evaluates a obscure selection problem hierarchically, designer's intention can be reflected in the design systematically. Also as qualitative attributes can be rated at quantitative criterion the designer's intention can be reflected consistently. Usually an engineering problem is a coupled problem in which a designer must select one alternative from a set of alternatives and find optimal characteristics of the alternative concurrently. As considered attributes are functions of the compromise system variables and the attributes's units and orders are different each other, attribute ratings must be normalized. This paper introduces a neural network at this normalization. So the attribute ratings can reflect designer's intention and the knowledge from his(her) experience automatically.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.7
• /
• pp.3061-3065
• /
• 2015

Background: Rac3, a member of the Rac family of small guanosine triphosphatases (GTPases), regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. Overexpression of Rac3 has been reported in several human cancers. However, the role of Rac3 in lung cancer (LC) has not been determined in detail. The purpose of this study was to investigate the effect of silencing of Rac3 expression in human LC cells and the consequences for cell survival. Materials and Methods: Lentivirus small hairpin RNA (shRNA) interference techniques were utilized to knock down the Rac3 gene. Gene and protein expression was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. LC cell apoptosis was examined by annexin V-APC /propidium iodide staining. Results: Efficient silencing of Rac3 strongly inhibited A549 cell proliferation and colony formation ability, and significantly decreased tumor growth. Moreover, flow cytometry analysis showed that knockdown of Rac3 led to G2/M phase cell cycle arrest as well as an excess accumulation of cells in the G1 and S phase. Conclusions: Thus, functional analysis using shRNAs revealed a critical role for Rac3 in the tumor growth of LC cells. shRNA silencing of Rac3 could provide an effective strategy to treat LC.

• Animal cells and systems
• /
• v.13 no.1
• /
• pp.71-78
• /
• 2009

To clarify the reproductive function of vibration signals in Hynobius, which has externally fertilized eggs, we quantitatively analyzed the body and tail undulations of male Korean salamanders (Hynobius leechii) in sixteen mating events. One large and one small male, and one female were used in each mating event. We analyzed behaviors recorded over a total of 3 hrs for each mating event; 2 hrs before and 1 hr after female's oviposition. Males touched females using their snouts or body trunk throughout the entire mating periods, but females touched males increasingly more after approximately 1 hr before oviposition. Males conducted body undulations more than 50 times per 10 minutes at a mean frequency of 0.64 Hz. Large males conducted more body undulations than small males, particularly on the tree twig where females attached their egg sacs. Males responded to other males' body undulation throughout the mating period by orienting their head towards, approaching, and touching the undulating male. Females only responded for about 10-20 min before ovipositing, and most responses were directed to the large male's body undulation. Males conducted tail undulations 3.0 times per 10 min at a mean frequency of 1.7 Hz and most tail undulations occurred after one male bit the other male. These results suggest that body undulations function in both male-female and male-male interactions, while tail undulations mainly functions in male-male competition. Also, male H. leechii appear to actively attract females, while females respond to the males only at times close to oviposition.

• The Korean Journal of Ecology
• /
• v.18 no.3
• /
• pp.307-321
• /
• 1995

Division of ecoregions having respective functions was attempted through quantitative and qualitative analysis on vegetation diversity, and heterogeneity and on soil environment of the study sites. Field research was carried out in a square of 81 ㎢ around Andongpo (126°38'E, 37°30'N), Kimpo-gun, Kyonggi provice. Conventional methods applied are as follows: classical syntaxonomy by the Zurich-Montpellier School, interpolation method to determine the degree of diversity, heterogeneity and distribution pattern of vegetation, and correlation analysis between soil properties and plant communities. 41 plant communities were identified and composed of 6 forests, 4 mantle and 31 herb communities including 6 saltmarsh plant communities. In a mesh, number of plant communities was highly correlated to the number of species. The highest number of plant community and species was 25 communities·km-2·mesh-1 and 381 species· km-2·mesh-1 ,and the highest value of vegetation heterogeneity was 28.1 species· community-1·mesh-1. Their lowest numbers were 4 communities·km-2·mesh-1. and 28 species·km-2·mesh-1. and 7 species·community-1·mesh-1, respectively. Contour map on vegetation diversity and heterogeneity enabled us to establish two regions; coastal and inland vegetation. Isoline 〔150〕,〔10〕and〔10〕and〔15〕on the species diversity, the community diversity and the vegetation heterogeneity, respectively, were regarded as ecolines in the study area. Cl- content was recognized as the most important factor from correlation analysis between soil properties. Ordination of sites indicated that the study area be divided into two edaphic types: inland and coastal habitats. It was considered that the extent of desalinization in soil played a major role in determining the species composition in the reclamed area. By matching edaphic division of habitats with division of vegetation structures, designation of ecoregion was endorsed. The approach of current study was suggested as an effective tool to implement an assessment of the vegetation dynamics by the disparity of natural environment and anthropogenic interferences.

• Journal of Environmental Science International
• /
• v.20 no.9
• /
• pp.1205-1212
• /
• 2011

As serving people's activities, pedestrian road systems are basic and necessary facilities in urban structure. To provide and utilize these pedestrian road systems in residential area would enhance urban environment as well as quality of life. For this reason, pedestrian road should be planned by consideration of people's activity in residential area. Evaluation of existing pedestrian road should be also oriented how people use it and what people do in it. This study amis to investigate functions of pedestrian road system throughout evaluation of user's satisfaction in order to improve better pedestrian road system in residential area. The purposes of this study are to analyze components of factors affecting on user's satisfaction, and to find the relationship among affecting factors. For this study, the on-site questionnaire method was applied to 267 individuals who were collected as the study areas where locate Toyogaoka and Kaidori, Japan. The collected data were clarified exploratory factors, and analyzed relationship between the factors and satisfaction by applying quantitative statistical techniques for the mapping investigation, Mann-Whitney u-test, and correlation. The results of this study are follows. The pedestrian road system is more preferred than surroundings of vehicle roads in residential district area where maintains pedestrian road as open space. In addition, satisfactions of the pedestrian road for each purpose were highly evaluated, because of conformability and convenience for usage. Consequently, the pedestrian road which is secure and greening as an open space is well carried out for the living circulation of residents. It would suggest that pedestrian road have to be managed and planned not a function of circulation but an open space system.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.8 no.4
• /
• pp.233-239
• /
• 2003

For the quantitative analysis of an unknown sample a calibration curve should be obtained, as analytical instruments give relative, rather than absolute measurements. Therefore, researchers should make standard samples with various known concentrations, measure each standard and the unknown sample, and then determine the concentration of the unknown by comparing the measured value to those of the standards. These procedures are tedious and time-consuming. Therefore, we developed a polymer based microfluidic device from polydimethylsiloxane, which integrates serial dilution and capillary electrophoresis functions in a single device. The integrated microchip can provide a one-step analytical tool, and thus replace the complex experimental procedures. Two plastic syringes, one containing a buffer solution and the other a standard solution, were connected to two inlet holes on a microchip, and pushed by a hydrodynamic force. The standard sample is serially diluted to various concentrations through the microfluidic networks. The diluted samples are sequentially introduced through microchannels by electro-osmotic force, and their laser-induced fluorescence signals measured by capillary electrophoresis. We demonstrate the integrated microchip performance by measuring the fluorescence signals of fluorescein at various concentrations. The calibration curve obtained from the electropherograms showed the expected linearity.

• BMB Reports
• /
• v.47 no.5
• /
• pp.262-267
• /
• 2014

Bcl-2 interacting cell death suppressor (Bis) has been shown to have anti-apoptotic and anti-stress functions. Recently, increased Bis expression was reported to correlate with glioma aggressiveness. Here, we investigated the effect of Bis knockdown on the acquisition of the invasive phenotype of A172 glioma cells, induced by 12-O-Tetradecanoylphorbol-3-acetate (TPA), using a Transwell assay. Bis knockdown resulted in a significant decrease in the migration and invasion of A172 cells. Furthermore, Bis knockdown notably decreased TPA-induced matrix metalloproteinase-9 (MMP-9) activity and mRNA expression, as measured by zymography and quantitative real time PCR, respectively. A luciferase reporter assay indicated that Bis suppression significantly down-regulated NF-${\kappa}B$-driven transcription. Finally, we demonstrated that the rapid phosphorylation and subsequent degradation of $I{\kappa}B-{\alpha}$ induced by TPA was remarkably delayed by Bis knockdown. These results suggest that Bis regulates the invasive ability of glioma cells elicited by TPA, by modulating NF-${\kappa}B$ activation, and subsequent induction of MMP-9 mRNA.

• Journal of the Korean Society of Costume
• /
• v.67 no.4
• /
• pp.38-57
• /
• 2017

Traditional costume in Indonesia represents identity of a person and it displays the origin and the status of the person. Where culture and religion are fused, the traditional costume serves one of the most functions in rituals in Bali. This research aims to analyze the characteristics of Balinese costumes by focusing on the Legong dance costume. Quantitative research was performed using 332 images of Indonesian costumes and 210 images of Balinese ceremonial costumes. Qualitative research was performed by doing field research in Puri Saba, Gianyar and SMKN 3 SUKAWATI(Traditional Art Middle School). The paper illustrates the influence and structure of Indonesian traditional costume. As the result, focusing on the upper wear costume showed that the ancient era costumes were influenced by animism. They consist of tube(kemben), shawl(syal), corset, dress(terusan), body painting and tattoo, jewelry(perhiasan), and cross. The Modern era, which was shaped by religion, consists of baju kurung(tunic) and kebaya(kaftan). The Balinese costume consists of the costume of participants and the costume of performers. Bali dancing is grouped into Wali dance(sacred), Bebali dance(theatrical), and Balih-balihan dance(entertainment). The Legong dance is included in the Balih-balihan dance, and its costume developed from 1920 until present. The characteristics of Legong dance costumes are 'Theatrical,' 'Angelic,' 'Charming,' and 'Decorative.' In conclusion, the balance of religion, culture, and art gives a unique trait in Bali. The Balinese social system, which is based on Hinduism, has influenced art and its function. This relationship creates a strong structure to the Balinese ceremonial costume, especially the Legong dance costume.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.9
• /
• pp.4071-4075
• /
• 2015

Background: Retinoblastoma protein-interacting zinc finger gene 1(RIZ1) functions as a tumor suppressor. Hypermethylation-mediated RIZ1 silencing has been reported in several cancers, but not in renal cell carcinoma (RCC) yet. Materials and Methods: We examined the RIZ1 expression and methylation in a panel of RCC cell lines and 50 primary tumors using semiquantitative/quantitative polymerase chain reaction (PCR), methylation specific PCR, and bisulfite sequencing genomic. We also explored the relationship between methylation status of RIZ1 and clinicopathological features in RCC patients. Results: RIZ1 expression was down-regulated or lost in OS-RC-2, 769-P, Caki-1, 786-O and A498 RCC cell lines. Restored expression of RIZ1 was detected after addition of 5-aza-2'-deoxycytidine with/without trichostatin A, suggesting that DNA methylation directly mediates its silencing. The RIZ1 expression was significantly reduced in RCCs compared to adjacent non-malignant renal samples (P<0.001). Aberrant methylation was detected in 15 of 50 (30%) RCCs and in 2 of 28 (7%) adjacent non-malignant renal samples (P=0.02). No statistically significant correlation between methylated and unmethylated cases with regard to age, gender, pathological stage and grade was observed. Conclusions: RIZ1 expression is down-regulated in human RCC, and this down-regulation is associated with methylation. RIZ1 methylation may play a role in renal carcinogenesis.

• Journal of mucopolysaccharidosis and rare diseases
• /
• v.1 no.2
• /
• pp.54-59
• /
• 2015

Purpose: Growth hormone (GH) therapy substantially improves several cognitive functions in PWS. However, the molecular mechanisms underlying the beneficial effects of GH on cognition remain unclear in PWS. In this study, we investigated the effects of recombinant human GH on the gene expression of GABAB receptor subunits and GH/insulin-like growth factor (IGF) axis genes in the brain regions of PWS-mimicking mice (Snord116del). Methods: Snord116del mice were injected subcutaneously with 1.0 mg/kg GH or saline, once daily for 7 days. The collected brain tissues were analyzed for mRNA content using quantitative PCR (qPCR) in the cerebellum, hippocampus, and cerebral cortex. Results: GH increased the mRNA expression level of the $GABA_{B1}$ receptor subunit ($GABA_{BR1}$) and IGF-1R in the cerebellum. Furthermore, a significant positive correlation was found between the level of $GABA_{BR1}$ mRNA and the expression of the IGF-1R transcript. GH also induced an increase in the mRNA expression of IGF-2 and IGF-2R in the cerebellum. Conclusion: These data indicate that GH may provide beneficial effects on cognitive function through its influences on the expression of $GABA_{BR1}$ and GH/IGF-1 axis genes in PWS patients.