• Food Science of Animal Resources
• /
• v.29 no.1
• /
• pp.108-113
• /
• 2009

Apolipoprotein E (APOE) is a plasma lipoprotein in mammals and plays an important role in the transport and metabolism of lipids such as phospholipids and triglycerides. Therefore, the APOE gene could be a candidate gene controlling lipid metabolism in beef cattle. This study was performed to identify single nucleotide polymorphisms (SNP) in the APOE gene and to investigate the effects of SNP genotype on the carcass traits such as meat quantity and quality in Korean cattle. For PCR amplification, pooled DNA made from unrelated 60 individuals was prepared and primer pairs were designed based on the cDNA sequence of exon 4 region of the bovine APOE gene. A SNP was identified at position 2034 (T/C substitution) of the exon 4 region in the APOE gene. PCR-RFLP procedure with restriction enzyme ACC I was developed for determining the SNP genotype for each of a total of 309 animals with pedigree information and performance records through the national progeny testing program. The frequencies of the genotypes TT, TC and CC were 10.9, 46.9 and 42.2%. Gene frequencies were 0.344 for T allele and 0.656 for C allele. The g.2034T>C SNP genotype showed a significant effect (p<0.05) on dressing percentage and meat color, respectively. Animals with the TT genotype showed higher dressing percentage than those with the CC genotype, and TT genotype had desirable meat color compared with CC genotype. These results suggest that the g.2034T>C SNP genotype of the APOE gene may be useful as a DNA marker for meat quantity index and dressing percentage in Korean cattle.

• Journal of Plant Biotechnology
• /
• v.6 no.1
• /
• pp.1-7
• /
• 2004

Forestry sector has witnessed some unprecedented events in the recent past both in terms of galloping biotechnological developments and heated environmental debates over risks associated with release of transgenic trees. Improvements in the in vitro propagation techniques has made it possible to develop tissue culture based plant regeneration protocols just for about any tree species. And with the inclusion of every new species within the realms of tissue culture technology, it becomes a candidate for genetic improvement through recombinant DNA technology, the so called genetic engineering. Poplars and their hybrids serve as the model tree species on which most of the genetic transformation work as been carried out. A lot of work has also gone in genetic transformation of fruit trees and trees of horticultural interests. Trees have been successfully transformed for traits ranging from reduction of length of juvenile phase to alteration of tree architecture to altering wood quality by lignin and cellulose modification. More-over trees have been genetically engineered successfully to combat various types of insect pests and pathogens causing diseases. But all these developments have ignited controversies over the possible benefits and risks associated with transgenic plantations by various environmental agencies and activists. Solutions to most of these concerns can be found out with more intensive prioritized research.

• Asian-Australasian Journal of Animal Sciences
• /
• v.5 no.3
• /
• pp.439-447
• /
• 1992

Eight healthy Holstein bulls, 4-6 years old were used to study the effect of season of the year on the biophysical characteristics of semen. Semen was collected twice a week by AV (artificial vagina) over one-year period. The analyses revealed that all the basic seminal traits studied were differed significantly due to season, except the ejaculate volume and consistency and the percentage of swollen spermatozoa in a hypo-osmotic fructose-citrate solution. Ejaculates collected during hot summer season had significantly lower sperm motility, concentration and total counts, and higher percentage of dead spermatozoa than those collected during winter time. Warm spring had moderate semen quality. The temperature-humidity index was calculated and it was associated (p < 0.01) negatively with the ejaculate pH, sperm concentration and total counts, and positively with the % of dead sperms. Ejaculate volume, percentage of swollen spermatozoa, individual motilities did not correlate significantly with the change in temperature-humidity index values. The total live, motile spermatozoa per ejaculate during both the winter and spring seasons showed significant increase of about 37% and 32% respectively over the summer season. Also, rectal temperatures of the bulls were elevated during the hot summer season, while the values of blood hemoglobin and packed-cell volume were decreased.

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.6
• /
• pp.914-918
• /
• 2018

Objective: The relationship between water-holding capacity (WHC) and intermuscular fat (IMF) was studied in Japanese commercial pork. Methods: Longissimus muscles of pigs (n = 62), obtained from two meat packing plants, were analyzed for IMF content, moisture content, drip loss, cooking loss, and pH. Pairwise relationships among these traits were determined using correlation analyses. Results: IMF content was significantly correlated with moisture content (r = -0.88; p<0.01) and pH (r = 0.32; p<0.05), but not with drip loss (r = -0.23; p = 0.07) or cooking loss (r = -0.10; p = 0.42). In contrast, drip loss was significantly (and negatively) correlated with pH (r = -0.57; p<0.01). Conclusion: IMF content was not significantly correlated with WHC in pork, and so ultimately, we consider pH to be one of the most important factors influencing WHC in pork meat.

• Journal of Korea Multimedia Society
• /
• v.20 no.8
• /
• pp.1175-1186
• /
• 2017

Human emotion recognition is one of the promising applications in the era of artificial super intelligence. Thus far, facial expression traits are considered to be the most widely used information cues for realizing automated emotion recognition. This paper proposes a novel facial expression recognition (FER) method that works well for recognizing emotion from image sequences. To this end, we develop the so-called weighted soft voting classification (WSVC) algorithm. In the proposed WSVC, a number of classifiers are first constructed using different and multiple feature representations. In next, multiple classifiers are used for generating the recognition result (namely, soft voting) of each face image within a face sequence, yielding multiple soft voting outputs. Finally, these soft voting outputs are combined through using a weighted combination to decide the emotion class (e.g., anger) of a given face sequence. The weights for combination are effectively determined by measuring the quality of each face image, namely "peak expression intensity" and "frontal-pose degree". To test the proposed WSVC, CK+ FER database was used to perform extensive and comparative experimentations. The feasibility of our WSVC algorithm has been successfully demonstrated by comparing recently developed FER algorithms.

• The Plant Pathology Journal
• /
• v.34 no.1
• /
• pp.35-43
• /
• 2018

Root-knot nematodes (Meloidogyne spp.) are parasites that attack many field crops and orchard trees, and affect both the quantity and quality of the products. A root-colonizing bacterium, Pseudomonas chlororaphis O6, possesses beneficial traits including strong nematicidal activity. To determine the molecular mechanisms involved in the nematicidal activity of P. chlororaphis O6, we constructed two mutants; one lacking hydrogen cyanide production, and a second lacking an insecticidal toxin, FitD. Root drenching with wild-type P. chlororaphis O6 cells caused juvenile mortality in vitro and in planta. Efficacy was not altered in the fitD mutant compared to the wild-type but was reduced in both bioassays for the mutant lacking hydrogen cyanide production. The reduced number of galls on tomato plants caused by the wild-type strain was comparable to that of a standard chemical nematicide. These findings suggest that hydrogen cyanide-producing root colonizers, such as P. chlororaphis O6, could be formulated as "green" nematicides that are compatible with many crops and offer agricultural sustainability.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.6
• /
• pp.811-818
• /
• 2017

Objective: This experiment was conducted to investigate the effects of fungal treated oil palm fronds (FTOPF) on performance, carcass traits, meat quality, and muscle chemical composition. Methods: Eighteen growing crossbred male goats (Thai Native${\times}$Anglo Nubian) with $18.7{\pm}2.0kg$ of initial body weight (BW) were stratified and blocked by BW in a randomized complete block design. Three diets containing 30% of oil palm fronds (OPF) either untreated (UOPF) or treated with Lentinussajor-caju (FTOPF) with or without urea (FTOPFU) were used as roughage sources in total mixed rations (TMRs). The diets were offered ad libitum and weight gain was determined. At the end of the experimental period, the harvest data and carcass characteristics of the goats were recorded, and muscular longissimus dorsi composition was determined. Results: No significant effect of fungal treated (FT) inclusion was observed in any of the feed intake, growth performance, and carcass characteristics. Likewise, no apparent effects on carcass composition and muscle chemical composition were detected in this study, except for hind leg and chump were affected (p<0.05) by FT inclusion. Conclusion: In conclusion, feeding of fungal (Lentinussajor-caju) treated oil palm frond in TMR diet did not affect performance and carcass characteristics in finishing goats.

• Journal of Animal Science and Technology
• /
• v.47 no.3
• /
• pp.391-396
• /
• 2005

이 연구는 거세가 제주 수말 비육시 성장 및 육질에 미치는 영향을 구영하기 위해 수행되었다. 24필(16-20개월령 )의 제주마를 12두씩 대조구(비거세)와 거세구에 3반복 (반복 당 4두)으로 배치하여 380일 동안 비육 시험을 실시하였다. 건초와 물은 자유급식 시켰고, 농후사료는 초기 260일 동안 체중의 1.25%, 후기 120 일 동안은 체중의 1.5% 급여하였다. 일당증체량과 사료섭취량은 처리 간 차이가 없었다. 등심단면적은 거세시 다소 증가되었고(P < 0.05), 근내지방도도 유의적 차이는 없었지만(P=0.08) 거세에 의해서 개선되는 경향을 보 였다{2.10 vs 1.59). 거세에 의해서 말고기 등심의 조지방 힘량은 증가된(P < 0.01) 반면, 수분과 조단백질 함량 은 감소되는 경향을 보였다 등심의 육색, pH, 가열감량 및 보수력은 거세 유무에 따른 차이를 보이지 않았 으나， 전단력은 거세구가 다소 감소하는 경향을 보였다(5.2vs 6.4 kg / 1.0-cm diameter core). 말고기 관능평가 에서는 거세구가 비거세에 비해 다즙성 연도 및 향미에서 개선된 경향을 보였다. 이 연구 결과, 제주마를 거 세 비육함으로써 근내지방도를 증가시켜 말고기의 육질을 개선시킬 수 있을 것으로 사료된다.

• The Journal of Korean Medicine
• /
• v.31 no.4
• /
• pp.171-177
• /
• 2010

Objective: To obtain an overview of the status of health-associated factors of Korean high school adolescents. Methods: 2,455 high school students (1,808 boys and 647 girls) filled out questionnairesfor their health-related factors such as physical problems, sleep and exercise. Results: Students sleep about 5.5 hours per day while they exercise only 2.6 hours each week. 14.2% of students skip breakfast every day. 31.0% of students were under severe stress, and 46.4 % has at least one symptom of distresses such as indigestion, headache, frequently catching cold, or muscle pain. 40.7% of the students took a functional supplement, especially vitamins as the most common, and ginseng and herbal drugs as the second and third. Conclusion: These results present general health-associated status of high school students, thus they could be helpful to design medicinal strategies for high school students using traditional Korean medicine.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.14 no.1
• /
• pp.23-32
• /
• 2007

The nature of genetic variation and diversity among the 65 multivoltine silkworm genotypes was evaluated for 16 post cocoon characters. The components of genetic variation revealed higher PCV (60.487%) and GCV (44.56%) for evenness (variation 1) followed by cohesion (PCV=55.38%, GCV=40.36%) and non-broken filament length (PCV=32.05%, GCV=31.28%). The higher heritability ($h^2$ in broad sense) was observed for boil-off loss (95.6%) followed by non-broken filament length (95.22%). The both genotypic and phenotypic correlation indicated significant positive correlation of filament length with non-broken filament length, silk recovery, raw silk, neatness, and low neatness; and negative correlation with denier, renditta and silk waste. The principal component analysis (PCA) revealed 75.381 % of total variance from the five principal components extracted. On the basis of Mahalonobis' $D^2$ values (Ward's minimum variance), the sixty-five multivoltine silkworm genotypes were classified in to 9 clusters with substantial inter and intra cluster distances. Number of genotypes included in different clusters varied from 3 to 17. The results indicated that the optimum distance obtained in cluster VII (15.059) along with higher cluster mean values especially for filament length, non broken filament length, renditta, silk recovery, silk waste, and raw silk emphasized the utilization of these genotypes in the conventional silkworm breeding programme for improvement of multivoltine silk fibre quality. The possibility of exploiting genetic variation in post cocoon traits for efficient breeding programme is discussed.