The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Hydrogen Cyanide Produced by Pseudomonas chlororaphis O6 Exhibits Nematicidal Activity against Meloidogyne hapla

  • Kang, Beom Ryong (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Anderson, Anne J. (Department of Biology, Utah State University) ;
  • Kim, Young Cheol (Institute of Environmentally-Friendly Agriculture, Chonnam National University)
  • Received : 2017.06.05
  • Accepted : 2017.11.23
  • Published : 2018.02.01
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Abstract

Root-knot nematodes (Meloidogyne spp.) are parasites that attack many field crops and orchard trees, and affect both the quantity and quality of the products. A root-colonizing bacterium, Pseudomonas chlororaphis O6, possesses beneficial traits including strong nematicidal activity. To determine the molecular mechanisms involved in the nematicidal activity of P. chlororaphis O6, we constructed two mutants; one lacking hydrogen cyanide production, and a second lacking an insecticidal toxin, FitD. Root drenching with wild-type P. chlororaphis O6 cells caused juvenile mortality in vitro and in planta. Efficacy was not altered in the fitD mutant compared to the wild-type but was reduced in both bioassays for the mutant lacking hydrogen cyanide production. The reduced number of galls on tomato plants caused by the wild-type strain was comparable to that of a standard chemical nematicide. These findings suggest that hydrogen cyanide-producing root colonizers, such as P. chlororaphis O6, could be formulated as "green" nematicides that are compatible with many crops and offer agricultural sustainability.

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References

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