• Title/Summary/Keyword: Quality culture

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Virus-Resistance Analysis in Transgenic Tobacco Expressing Coat Protein Gene of Cucumber Mosaic Virus (오이모자이크바이러스 외피단백질유전자 발현 담배의 바이러스 저항성 분석)

  • 손성한;김경환;박종석;황덕주;한장호;이광웅;황영수
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.3
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    • pp.153-160
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    • 1997
  • Cucumber mosaic virus (CMV) leads to a cause of poor crop productivity and quality. To solve this problem, we attempted to develop a virus-resistance tobacco plants by using viral coat protein (CP) gene. Transgenic tobacco plants expressing CMV CP gene were analysed by the resistance upon CMV infection. The virus-resistance was measured in $\textrm{T}_{1}$, generation by the inhibition of plant growth and the expression of the mosaic symptoms infected with CMV. The transgenic lines were divided into four groups: highly resistant, resistant, moderate and susceptible based on their growth and symptom severity. Out of 39 transgenic lines, 16 lines showed significant virus-resistance. And of resistant lines, 2 lines were designated highly resistant based on the facts that they achieved similar plant height to that of non-infected tobacco plants and showed lower disease symptom than that of other lines. The steady state level of CP RNA and coat protein level were measured by northern blot and immunoblot analysis. The CP RNA was highly accumulated in most resistant and moderate lines but barely detected in susceptible lines. The coat protein was detected in most lines regardless of their resistance to CMV. from this result, virus-resistance appeared to correlate more with CP RNA level than the level of coat protein. However, in two highly resistant lines, CP RNA level was unexpectedly low. This unexpected phenomenon need to be further investigated.

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Effect of Watering on Growth and Essential Oil Content of Sweet Basil (Ocimum basilicum) (관수량이 sweet basil(Ocimum basilicum)의 생장과 정유함량에 미치는 영향)

  • Baeck, Hae-Won;Park, Kuen-Woo
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.81-86
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    • 2001
  • This experiment was carried out to investigate watering with relation to growth, quality and yield of essential oil in sweet basil. The degree of water stress was taken as amount of watering. D1 was watered with 25mL for 2 weeks and 30mL from 4 to 5 weeks after planting in Wagner pot (1/20000a, ${\phi}24cm$). As this, 75mL and 90mL, 225mL and 270mL, and 675mL and 810mL were watered in D2, D3, and D4 treatment respectively. DFT was set up as water stress was not inflicted. The growth of basil in D3 and D4 was better than that of others, in which root activity was as much twice as that of D1. Essential oil of D1 was recorded the lowest content as 0.33%. The result of proline content, peroxidase activity, photosynthesis, stomatal conductance and stomatal resistance were proved D1 to be stressed. This treatment consequently increased the content of essential oil. In consideration of growth and essential oil content, D3 treatment was highest as 47.37mg in oil production per plant. Finally, D3 watered with 225mL for 2 weeks and 275mL from 4 to 5 weeks after planting could be selected on the purpose of both plant growth and essential oil production. Essential oil content of sweet basil was increased in response to water stress. For increase of essential oil yield, oil synthesis could be raised by giving water stress just before harvesting.

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Acute Toxicity of Ozone on Survival and Physiological Conditions of Olive Flounder, Paralichthys Olivaceus (넙치(Paralichthys olivaceus)의 생존과 생리상태에 미치는 오존의 급성 독성)

  • Kim, Heung-Yun;Oh, Myung-Joo;Jung, Sung-Ju
    • Journal of fish pathology
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    • v.12 no.1
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    • pp.32-41
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    • 1999
  • Ozonization for rearing seawater in land-based culture system has recently been utilized for disinfection of pathogenic microorganisms and improvement of water quality. This study was conducted to examine the effects of total residual oxidants (TRO) in ozone-treated seawater on survival, blood parameters, osmolality and oxygen consumption, and gill tissue of the flounder. Paralichthys olivaceus. Experiments were carried out with the starved flounder of 12~19 cm in total length at $20^{\circ}C$. The 48-hr and 96-hr $LC_{50}$ for the flounder amounts to 26.4 ppb and 22.3 ppb, respectively. With increase of TRO concentration from 24 to 39 ppb, the values of hematocrit, hemoglobin concentration, red blood cell count and osmolality of the flounder with respect to exposure time were significantly elevated, however, the oxygen consumption rates decreased. In the case of the fish exposed to 13 ppb for 96 hrs, blood glucose increased with an elapse of exposure time, while survival rate was 100%. Death apparently resulted by massive destruction of gill lamellar epithelium, severe osmotic imbalance and the lack of oxygen uptake. The results of this experiment indicated that to protect aquaculture organisms, the ozone-treated seawater should not contain any residual oxidants, and that relatively long-term exposure to TRO of low concentration can impact on survival and physiological conditions of the flounder.

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Effect of the hatchery larval sieving on the larval growth, scuticociliate occurrence, and ensuing spat growth of Patinopecten yessoensis (참가리비, Patinopecten yessoensis 인공종묘 생산시 환수가 유생에 미치는 영향-유생성장, 스쿠티카충 발생, 치패성장의 관점)

  • Jo, Q-Tae;Kim, Su-Kyoung;Lee, Chae-Sung;Lee, Jin-Ho;Park, Mi-Seon;Moon, Tae-Suk
    • Journal of fish pathology
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    • v.23 no.3
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    • pp.303-311
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    • 2010
  • Our previous finding summarizing that larval sieving process is inevitable but triggers the outbreak of scutica-like ciliates (SLCs) in the seed production of Patinopecten yessoensis urged further study to determine best suggestable sieving interval in an agreeable range of water quality. In the mass seed production of the scallop, SLC outbreak was closely related to the larval sieving in which larvae were drained on the basis of every 3-day (5T), 5-day (3T), 7-day (2T), or 9-day (1T) from culture tanks onto a mesh screen and placed back into new water in cleaned tanks. The larval performance of growth and survival was clearly dependent on the sieving intervals. It was in order of 3T, 5T, 2T, and 1T for both of growth and survival and in reverse order for SLC infection frequency, confirming that larval sieving is necessary but damageable if it overwhelms the larval resistance. Interestingly, the larval damages by the sieving persisted to their ensuing spat life in terms of nursery growth, survival, and abnormality.

A Study on Difference between Korea and China Consumers in importance of Attributes, Shopping Value and Loyalty in Social Commerce (한국과 중국소비자의 소셜커머스 속성의 중요도, 쇼핑가치 및 충성도 차이에 관한 연구)

  • Kim, Moon-Jung
    • Journal of Distribution Science
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    • v.13 no.1
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    • pp.47-55
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    • 2015
  • Purpose - The objective of this study is to verify whether there are differences between Korean and Chinese consumers in the importance of the attributes such as shopping value and loyalty in social commerce. With the purpose of finding out these differences, the following questions are set up as research questions. First, will there be a difference between Korea and China in the importance of attributes of social commerce? Second, will there be a difference between Korean and Chinese consumers in shopping value? Third, will there be a difference between Korean and Chinese consumers in their loyalty? Research design, data, and methodology - The researchers collected data through self-administered questionnaires from Korean and Chinese consumers who had used social commerce within the past six months before answering the questionnaire. First, for social commerce users in Korea, comprising male and female university students in Chungcheong, a questionnaire was circulated. For social commerce users in China, university students within Beijing were surveyed. Responses to 336 questionnaires were used, excluding those with no answers or unreliable answers. Data coding and data cleaning were used. SPSS 18.0 was used. First, exploratory factor analysis is done to verify the validity of testing tools, and Cronbach's α coefficient is used to verify credibility. For factor analysis, the Varimax method is used. To verify the internal consistency reliability of each factor, the Cronbach's α coefficient is used to verify the credibility. Second, a T-test is done to verify differences between Korean and Chinese consumers for the importance of attributes. Third, ANOVA (Analysis of variance) is done to verify differences between Korean and Chinese consumers in shopping value and loyalty. Results - When Korean and Chinese consumers use social commerce, first, the importance of the attributes of social commerce is affected by four factors; playfulness and economic factors are more important to Korean consumers than Chinese consumers. As for informativeness factors, including product information and quality, and buyer comments, there was no confirmed difference between Korean and Chinese consumers. The convenience factor is more important to Chinese consumers than Korean consumers. Second, the factors affecting shopping value for Korean and Chinese consumers were hedonic shopping value and rational shopping value. To see the difference between Korean and Chinese consumers in shopping value factors, a t-test is conducted. As a result, in the rational shopping value factors of social commerce, Korean consumers scored higher than did Chinese consumers. These results were verified to be meaningful through statistics. In the hedonic shopping value factor of social commerce, Korean consumers scored higher than Chinese consumers. These results showed a significant difference. Third, loyalty in social commerce is higher for Chinese consumers than for Korean consumers. However, there is no difference in loyalty depending on sex. Conclusion - These results will hopefully be valuable and used in the future by Korean companies that wish to enter the Chinese social commerce market.

Growth and Flowering of Cut Spray Chrysanthemum 'Gama' by Daminozide (스프레이 절화국화 '가마'의 daminozide에 의한 생육 및 개화반응)

  • Lee, Chang Hee;Park, In Moon
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.2
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    • pp.121-127
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    • 2009
  • This study was conducted to improve commercial quality of a cut spray chrysanthemum 'Gama' bred in Korea by foliar application of daminozide (DMZ), suppressing excessive elongation of peduncle caused by high temperature in summer season production. Applications were made at three floral bud developmental stages (I, II, and III), and concentrations used were 0, 500, 1000, and $2000mg{\cdot}L^{-1}$. Both cut flower length and peduncle length showed the greatest suppression of elongation by DMZ treatments at stage II. DMZ also gradually reduced values of those in a concentration-dependant manner. Stem thickness and flower diameter reduced compared to control as DMZ was sprayed at later stage and those two characteristics decreased in a concentration-dependant manner. The number of paralleled flowers neighboring apical part of whole plant showed the best results at stage I and II. As for DMZ concentration, those increased effectively at $500-1,000mg{\cdot}L^{-1}$. Among all combinations, $1,000mg{\cdot}L^{-1}$ DMZ at stage II recorded the most paralleled flowers and showed 24.2% increment as compared to control. The number of fully expanded flowers and total floral buds also showed the highest values using $1,000mg{\cdot}L^{-1}$ DMZ at stage II than the other treatments. In cut spray chrysanthemum cultivar 'Gama' bred in Korea, foliar-spraying with $1,000mg{\cdot}L^{-1}$ DMZ at stage II most effectively suppressed excessive peduncle elongation, parallelled flower cluster arrangement, and increased the number of flowers in summer. However, this combination affected negatively the other commercial qualities, reducing cut flower length, stem thickness, and flower diameter. Therefore, we recommended that foliar-spraying stage and DMZ concentration was stage I and $1,000mg{\cdot}L^{-1}$, respectively, in a practical culture for 'Gama'.

Immature Ovule Culture of Lilium Oriental Interspecific crossings (오리엔탈 종간잡종 나리 미숙배주 배양)

  • Choi, Eun Gyung;Park, Byung Mo
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.221-225
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    • 2009
  • Interspecific crossing was conducted to obtain hybrid lilly with better quality of adapting to the unfavorable environment using oriental Lilium spp. as a female parent. Average interspecific crossing rate between oriental and asiatic lines by cut-style pollination was as low as 29%. Although the average germination rate of zygotic hybrid embryo between oriental 'Rodolfa' ${\times}$ asiatic 'Toronto' was increased from 76.6% to 78.3%. on 114 or 1/6 strength MS medium compared to the control, the rate of zygotic embryos formation between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' was significantly enhanced up about 86~90% on 1/2 strength MS medium. Meanwhile, germination rate of interspecific hybrid embryo between oriental 'Snow Cristal' ${\times}$ asiatic 'Royal Trinity' showed up 100%. Germination rate of interspecific hybrids slightly increased by addition of $0.5-1.0mg{\cdot}L^{-1}$ GA. Germination rate of zygotic embryos between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' and oriental 'Belcanto' ${\times}$ L. callosum was highest on the MS medium containing $0.01mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ kinetin, as 66.6% and 45.2% respectively.

An Analysis on Policy of Independent College using the Four-Dimensional Framework (중국의 독립학원 정책 분석 : 다차원 교육정책분석 모형을 중심으로)

  • Wu, Shan;Chung, Jae Young;Jang, Su Yeon
    • Korean Journal of Comparative Education
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    • v.27 no.1
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    • pp.171-197
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    • 2017
  • China's independent college approved private education investment, and facilitates the use of funds to support individual investors, corporations, and society. In contrast to China's public universities, the college guarantee private school method of operation. Its bachelor's degree, admission to students, the establishment of a separate corporation, and the recognition of scholastic achievements, was established with the aim of ensuring the diversity of higher education institutions in China. However, since the early 1990s, the independent college, which has emerged as a new way of higher education in China, has achieved quantitative growth over the past 30 years, but the quality of education has not yet grown. The reason why the independent college in China is interested is that it receives support from the facilities and professors of the original public college, and the major in which it is established and shares the reputation of the university. This study tried to analyze the policy of independent college which is a unique higher education institution in China. For this purpose, we use Four-Dimensional Framework to analyze the problem of China's independent colleges. It examines the profitability and non-profitability of independent college as a normative dimension and analyzes the Chinese society that have the old "guanxi" culture core in China. On the structural dimension, we analyzed the structure of the relationship in educational administrative institution. On the constituentive dimension, we observed that the various stakeholders who are interested in the independent college policy. Finally, we searched for future directions of the independent college centered on the process of legalization of independent colleges in technical dimension. The results of this analysis suggest the implications of the direction of China's independent college policy.

Demand for Classical Music Concerts from Transaction Cost Perspectives (거래비용 관점으로 본 클래식 음악공연 관람수요)

  • Lee, Chang Jin;Kim, Jaibeom
    • Review of Culture and Economy
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    • v.17 no.2
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    • pp.3-28
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    • 2014
  • The characteristics of performing arts differ from those of utilitarian goods in terms of economics. Factors other than price need to be considered to understand the demand for performing arts. Audience surveys as well as econometric demand studies have confirmed that socio-economic factors such as age, income, employment, and education are major determinants of the demand for performing arts. This study focused on the attributes of concerts rather than consumer characteristics to determine the concerts audiences select in terms of transaction cost. Genre, price, internet search trends, and the purpose of performance as well as price are tested as determinants of demand by using the data set for a major concert hall in Seoul. Genre and the specific purpose of concerts influence the demand for concerts. Internet search trends of the performer are used as indicators of popularity and information exposure, which are positively correlated with demand. This result supports the hypothesis that larger audiences would attend concerts that require lower information search costs. To note, price has a positive effect on demand in the higher price range, which means that concerts at higher prices attract larger audiences, whereas normal goods have a negative slope in the demand curve. This result can be explained by the hypothesis that consumers use price as an indicator of the quality expected of a concert. Transaction cost for selecting classical concerts thus forms an inverse-U shape curve against ticket price. These results provide some explanation of why audiences of classical music choose to attend concerts at high ticket prices while offering evidence in favor of the hypothesis that performing arts are selected in a social context.

Correlation between Characteristics of SOD in Coastal Sewage and Predictive Factor (연안 저질 SOD의 특성과 유발 영향인자에 대한 상관관계)

  • Kim, Beom-Geun;Khirul, Md Akhte;Kwon, Sung-Hyun;Cho, Dae-Chul
    • Korean Journal of Environment and Ecology
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    • v.33 no.5
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    • pp.596-604
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    • 2019
  • This study conducted a sediment culture experiment to investigate the effects of sediment oxygen demand (SOD) and environmental factors on sediment and water quality. We installed a leaching tank in the laboratory, cultured it for 20 days, and analyzed the relationship between P and Fe in the sediment. As a result, the dissolved oxygen of the water layer decreased with time, while the oxidation-reduction potential of the sediment progressed in the negative direction to form an anaerobic reducing environment. The SOD was measured to be 0.05 mg/g at the initial stage of cultivation and increased to 0.09 mg/g on the 20th day, indicating the tendency of increasing consumption of oxygen by the sediment. The change is likely to have caused by oxygen consumption from biological-SOD, which is the decomposition of organic matter accumulated on the sediment surface due to the increase of chl-a, and chemical-SOD consumed when the metal-reducing product produced by the reduction reaction is reoxidized. The correlation between SOD and causality for sediment-extracted sediments was positive for Ex-P and Org-P and negative for Fe-P. The analysis of the microbial community in the sediment on the 20th day showed that anaerobic iron-reducing bacteria (FeRB) were the dominant species. Therefore, when the phosphate bonded to the iron oxide is separated by the reduction reaction, the phosphate is eluted into the water to increase the primary productivity. The reduced substance is reoxidized and contributes to the oxygen consumption of the sediment. The results of this study would be useful as the reference information to improve oxygen resin.