Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Virus-Resistance Analysis in Transgenic Tobacco Expressing Coat Protein Gene of Cucumber Mosaic Virus

오이모자이크바이러스 외피단백질유전자 발현 담배의 바이러스 저항성 분석

  • 손성한 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 김경환 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 박종석 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 황덕주 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 한장호 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 이광웅 (서울대학교 자연과학대학 생물학과) ;
  • 황영수 (농촌진흥청 농업과학기술원 생물자원부 생화학과)
  • Published : 1997.05.01
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Abstract

Cucumber mosaic virus (CMV) leads to a cause of poor crop productivity and quality. To solve this problem, we attempted to develop a virus-resistance tobacco plants by using viral coat protein (CP) gene. Transgenic tobacco plants expressing CMV CP gene were analysed by the resistance upon CMV infection. The virus-resistance was measured in $\textrm{T}_{1}$, generation by the inhibition of plant growth and the expression of the mosaic symptoms infected with CMV. The transgenic lines were divided into four groups: highly resistant, resistant, moderate and susceptible based on their growth and symptom severity. Out of 39 transgenic lines, 16 lines showed significant virus-resistance. And of resistant lines, 2 lines were designated highly resistant based on the facts that they achieved similar plant height to that of non-infected tobacco plants and showed lower disease symptom than that of other lines. The steady state level of CP RNA and coat protein level were measured by northern blot and immunoblot analysis. The CP RNA was highly accumulated in most resistant and moderate lines but barely detected in susceptible lines. The coat protein was detected in most lines regardless of their resistance to CMV. from this result, virus-resistance appeared to correlate more with CP RNA level than the level of coat protein. However, in two highly resistant lines, CP RNA level was unexpectedly low. This unexpected phenomenon need to be further investigated.

오이 모자이크바이러스(CMV, cucumber mosaic virus)는 작물의 생산량과 품질에 심각한 피해를 주기 때문에 외피단백질 유전자(CP, coat protein gene)를 도입하여 저항성 작물를 개발하고자 하였다. CMV CP유전자가 도입된 형질전환 담배 39 계통을 대상으로 오이모자이크바이러스 저항성을 검정하였다. 바이러스 저항성은 바이러스 감염으로 인한 생장 억제정도, 병징발현에 따른 잎모양의 변화로서 고도저항성, 저항성, 중간성, 감수성 등으로 판정하였고 39개 계통중 16 계통이 뚜렷한 바이러스 저항성을 보였다. 특히, 저항성 계통중 2 계통은 생장량과 잎모양에서 다른 저항성 계통보다 우수하여 고도저항성으로 세분하였다. 각 형질전환계통에서 CP단백질과 CP RNA 생성량을 조사하였는바, CP단백질 생합성은 대부분의 저항성과 감수성계통에서 검출되어 저항성과 특별한 관련을 인정할 수 없었으나 CP RNA는 대부분의 저항성 및 중간성 계통에서 다량 축적되는 경향을 보여 CP RNA가 저항성에 좀더 밀접함을 알수 있었다. 그러나 고도저항성 계통에서는 CP RNA가 검출되지 않아 저항성의 근원을 파악하기 위해서는 계속적인 연구가 요구된다.

Keywords

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