• Journal of Microbiology and Biotechnology
• /
• v.34 no.3
• /
• pp.725-734
• /
• 2024

CYP102A1 from Bacillus megaterium is an important enzyme in biotechnology, because engineered CYP102A1 enzymes can react with diverse substrates and produce human cytochrome P450-like metabolites. Therefore, CYP102A1 can be applied to drug metabolite production. Terpinen-4-ol is a cyclic monoterpene and the primary component of essential tea tree oil. Terpinen-4-ol was known for therapeutic effects, including antibacterial, antifungal, antiviral, and anti-inflammatory. Because terpenes are natural compounds, examining novel terpenes and investigating the therapeutic effects of terpenes represent responses to social demands for eco-friendly compounds. In this study, we investigated the catalytic activity of engineered CYP102A1 on terpinen-4-ol. Among CYP102A1 mutants tested here, the R47L/F81I/F87V/E143G/L188Q/N213S/E267V mutant showed the highest activity to terpinen-4-ol. Two major metabolites of terpinen-4-ol were generated by engineered CYP102A1. Characterization of major metabolites was confirmed by liquid chromatography-mass spectrometry (LC-MS), gas chromatography-MS, and nuclear magnetic resonance spectroscopy (NMR). Based on the LC-MS results, the difference in mass-to-charge ratio of an ion (m/z) between terpinen-4-ol and its major metabolites was 16. One major metabolite was defined as 1,4-dihydroxyp-menth-2-ene by NMR. Given these results, we speculate that another major metabolite is also a mono-hydroxylated product. Taken together, we suggest that CYP102A1 can be applied to make novel terpene derivatives.

• Journal of Korean Society of Forest Science
• /
• v.105 no.3
• /
• pp.284-293
• /
• 2016

In order to assess th changes in vegetation characteristics over time in the forest-lands which is isolated by urbanization, vegetation surveys based on the Braun-Blanquet phytosociological method was carried out in 1996 and 2015 on Mt. Hwangyeong located in the center of Busan metropolitan city, South Korea. The number of vegetation types based on floristic composition showed no significant changes, the vegetation units under the community levels was more or less represents the difference. the average total vegetation cover and average number of species per unit area ($100m^2$) was increased 16% and 2 species, respectively. The relative importance value (RIV) for each tree species, Quercus spp. like as Q. mongolica and Q. serrata was decreased, while the warmth-tolerant trees, evergreen broad-leaved trees, and mesophyte like Lindera erythrocarpa, Styrax japonica, Osmanthus heterophyllus, and Stephanandra incisa was increased significantly. Changes of the life form spectrums of vascular plants did not substantially, hemicryptophyte(H) and therophyte (Th) was decreased, while nanophanerophyte(N) was increased significantly. Also through the creating of large-scale vegetation map (based on physiognomic vegetation types) was compared the spatial distribution characteristics of each vegetation types.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.8 no.3
• /
• pp.132-140
• /
• 2006

Forest vegetation classification and species composition of Mt. Ilwol, Yeongyang-Gun, Korea, were studied combining the Braun-Blanquet approach with numerical syntaxonomical analyses (TWINSPAN). Vegetation types and various ecological characteristics such as flora, constancy classes, species ratio of life-form, species diversity and importance value were analyzed. Sixty-eight samples were taken from a $100m^2$ square plot each. Forest communities were identified as two great types: arid landform of mountainside (AM) and humid fertility of piedmont and valley (HP). The former was divided into 3 communities (Rhododendron mucronulatum, Quercus variabilis, Hosta capitat community) and 2groups, and the latter into 3 communities (Tilia amurensis, Vitis coignetiae, Philadelphus schrenckii community) and 2 groups. Vegetation was classified into 8 units. Floristically, the most represented family was Compositae with 26 species. Species with percentage constance degree of more than 61% was Quercus mongolica (72.1%, IV); Carex siderosticat (III) and Fraxinus rhynchophylla (III) were 50.0 and 41.1%, respectively. Life-forms species ratios for trees, subtrees, shrub, vines, grominoids, forbs and ferns were 18.5, 5.7, 14.9, 6.6, 8.8, 42.4 and 3.1%, respectively, PH type showed from $1.70{\pm}0.50\;to\;1.97{\pm}0.57$ and AM type was from $1.40{\pm}0.18\;to\;1.62{\pm}0.20$ in species diversity; therefore, the former type showed higher species diversity than the latter, According to importance value analysis, Pinus densiflora, Quercus mongolica and Q. variabilis were higher in the tree layer, Q. mongolica in the subtree layer, Fraxinus sieboldiana, R. schlippenbachii, etc. in the shrub layer and Carex siderosticta, Carex humilis, etc. in the herb layer.

• Journal of the Korean Wood Science and Technology
• /
• v.32 no.4
• /
• pp.8-17
• /
• 2004

Antifungal, antibacterial, and antioxidative activities of ethanol extracts from 65 families 263 species were investigated to select tree species for the utilization of natural fungicide or preservative resources. The antifungal activities of extracts from wood, leaf and bark were measured as hyphal growth inhibition rate using four plant pathogenic and five wood rotting fungi. High inhibitory effect on the fungi growth was found in five species of Pinaceae (Pinus koraiensis, P. rigida, P. densiflora, P. banksiana. Cedrus deodara), three species of Cupressaceae (Juniperus rigida, J. chinensis, Chamaecyparis obtusa) and three species of Leguminosae (Albizzia julibrisssin, Sophora japonica, Maackia amurensis), respectively. Antibacterial activities of ethanol extracts were determined by means of disc-agar plate diffusion method using three gram-positive and five gram-negative bacteria. The ethanol extracts, which showed prominent effect on the suppression of bacteria growth, were six species of Betulaceae (Carpinus tschonoskii, C. coreana, C. laxiflora, Alnus hirsuta, A. firma, Betula schmidtii), five species of Fagaceae (Castanopsis cuspidata var. sieboldii, Quercus serrata, Q. mongolica, Q aliena, C crenata), four species of Euphorbiaceae (Aleurites fordii, Sapium sebiferum, S japonicum Mallotus japonicus) and three species of Elaeagnaceae (Elaeagnus umbellata, Elaeagnus glanbra, Elaeagnus macrophylla). According to these results, the extracts from Zelkova serrata, Pinus densiflora, Maackia amurensis, Chamaecyparis obtusa and Juniperus chinensis could be available for natural fungicide or food preservatives, because ethanol extracts from these species indicated excellent antifungal and antibacterial activities. In order to test antioxidative activities of ethanol extracts, free radical scavenging method was adopted with 1,1-diphenyl-2-picrylhydrohydrazyl (DPPH). Free radical scavenging activity was proved very high in the extracts of eight species of Rosaceae (Eriobotrya japonica, Prunus takesimensis, P yedoensis, P padus, P armeniaca var. ansu, Chaenomeles sinensis, Stephanandra incisa, Rosa multiflora) and five species of Ericaceae (Rhododenron mucronulatum, R. scblippenbacbii, R. yedoense var. poukhanense, Vaccinium bracteatum, V oldbami), resvectively. It turned out from this study that only six species among 48 species of Rosaceae showed less than 80% free radical scavenging activity. As a consequences, it could be deduced that the components effective on antioxidative activity commonly exist in Rosaceae plant family.