• Title/Summary/Keyword: Q&A System

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Purification of Neuropeptide with the Contractile Activity on the Smooth Muscle from the Skin of Conger Eel Conger myriaster (붕장어(Conger myriaster)의 피부로부터 평활근 수축작용을 지닌 신경성 펩타이드의 정제)

  • Go, Hye-Jin;Park, Nam-Gyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.4
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    • pp.358-366
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    • 2012
  • A novel neuropeptide was isolated from the skin of the conger eel Conger myriaster using hagfish Eptatretus burgeri intestine as a bioassay system. The sequence of the purified peptide was analyzed using automated amino acid sequencing and MALDI-TOF mass spectrophotometry. The molecular ion peak in the MALDI-TOF mass spectrum of the peptide was at m/z 962.89 $(M+H)^+$. The sequence of the peptide was determined to be L-P-M-L-E-T-Q-M, and was tentatively named comyrin. To investigate the complete primary structure of comyrin, comyrin-OH and comyrin-$NH_2$ were synthesized and the chemical and pharmacological properties of the synthetic peptides were compared with those of the native peptide. However, the elution time of synthetic peptides did not match that of the native peptide on the reverse-phase HPLC chromatogram. In addition, the synthetic peptides did not cause contractile activity in the intestinal smooth muscle of the hagfish. Based on these results, one possible reason for this disagreement may be the presence of a D-amino acid in comyrin.

A Study for Development Plan of SAR Core Technology Through Technology Readiness Level Survey and Analysis (기술 수준조사 및 분석을 통한 SAR(합성개구면 레이다) 핵심기술 개발방안 연구)

  • Kwak, Jun-Young;Jeong, Dae-Gwon
    • Journal of the Korea Institute of Military Science and Technology
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    • v.14 no.4
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    • pp.655-662
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    • 2011
  • SAR(Synthetic Aperture Radar) has the ability to generate high resolution images regardless of a weather condition(e.g. visibility good or poor and day or night, etc.). SAR is considered as one of the most important powers and needs for the future since it has been utilized in a number of important military fields such as early warning, urban defense, missile guidance system, etc. Additionally there are many civilian demands and applications in aviation, traffic control, earth and space explorations, weather forecast etc. This days, the ability to acquire and analyze information is needed to cope with the urgency of global politics and international changes. In this paper, technical survey and development review for SAR systems are investigated to derive the core and immature technologies of domestic defense industry.

Expression of Mosquitocidal Bacillus sphaericus Binary Toxin and B. thuringiensis cry11B Genes in B. thuringiensis 407

  • Park, Hyun-Woo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.185-189
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    • 2001
  • Wild type Bacilus thuringiensis subsp. israelensis and B. sphaericus toxins have been used separately as active in ingredients for bacterial insecticides to control mosquito larvae due to their comparable toxicity to chemical insecticides. Cry11B, recently cloned from B. thuringiensis subsp. jegathesan, shows higher toxicity against three major species of mosquito larvae than Cry11A, one of the major component of B. thuringiensis subsp. israelensis inclusion body. To determine whether the combination of cry11B and B. sphaericus binary toxins is as toxic as B. thuringiensis subsp. israelensis parental strain, cry11B and B. sphaericus binary toxins genes were co-expressed as an operon using cytlA promoters/STAB-SD hybrid expression system in B. thuringiensis subsp. israelensis acrystalliferous strain 4Q7. However, unexpectedly, B. sphaericus binary toxins were barely produced, whereas relatively large amount of Cry11B was produced. When this strain was grown in four different media, NB+G and Peptonized Milk produced more toxin proteins and spores per unit of media than GYS and G-Tris. Toxicity of this strain against fourth instar Culex quinquefasciatus was ranged from of 8.3 to 45.7 ng/ml, with NB+G culture being the highest, and GYS culture was the lowest.

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Study on the spectroscopic reconstruction of explosive-contaminated overlapping fingerprints using the laser-induced plasma emissions

  • Yang, Jun-Ho;Yoh, Jai-Ick
    • Analytical Science and Technology
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    • v.33 no.2
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    • pp.86-97
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    • 2020
  • Reconstruction and separation of explosive-contaminated overlapping fingerprints constitutes an analytical challenge of high significance in forensic sciences. Laser-induced breakdown spectroscopy (LIBS) allows real-time chemical mapping by detecting the light emissions from laser-induced plasma and can offer powerful means of fingerprint classification based on the chemical components of the sample. During recent years LIBS has been studied one of the spectroscopic techniques with larger capability for forensic sciences. However, despite of the great sensitivity, LIBS suffers from a limited detection due to difficulties in reconstruction of overlapping fingerprints. Here, the authors propose a simple, yet effective, method of using chemical mapping to separate and reconstruct the explosive-contaminated, overlapping fingerprints. A Q-switched Nd:YAG laser system (1064 nm), which allows the laser beam diameter and the area of the ablated crater to be controlled, was used to analyze the chemical compositions of eight samples of explosive-contaminated fingerprints (featuring two sample explosive and four individuals) via the LIBS. Then, the chemical validations were further performed by applying the Raman spectroscopy. The results were subjected to principal component and partial least-squares multivariate analyses, and showed the classification of contaminated fingerprints at higher than 91% accuracy. Robustness and sensitivity tests indicate that the novel method used here is effective for separating and reconstructing the overlapping fingerprints with explosive trace.

Forisome based biomimetic smart materials

  • Shen, Amy Q.;Hamlington, B.D.;Knoblauch, Michael;Peters, Winfried S.;Pickard, William F.
    • Smart Structures and Systems
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    • v.2 no.3
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    • pp.225-235
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    • 2006
  • With the discovery in plants of the proteinaceous forisome crystalloid (Knoblauch, et al. 2003), a novel, non-living, ATP-independent biological material became available to the designer of smart materials for advanced actuating and sensing. The in vitro studies of Knoblauch, et al. show that forisomes (2-4 micron wide and 10-40 micron long) can be repeatedly stimulated to contract and expand anisotropically by shifting either the ambient pH or the ambient calcium ion concentration. Because of their unique abilities to develop and reverse strains greater than 20% in time periods less than one second, forisomes have the potential to outperform current smart materials as advanced, biomimetic, multi-functional, smart sensors or actuators. Probing forisome material properties is an immediate need to lay the foundation for synthesizing forisomebased smart materials for health monitoring of structural integrity in civil infrastructure and for aerospace hardware. Microfluidics is a growing, vibrant technology with increasingly diverse applications. Here, we use microfluidics to study the surface interaction between forisome and substrate and the conformational dynamics of forisomes within a confined geometry to lay the foundation for forisome-based smart materials synthesis in controlled and repeatable environment.

8 Antenna Polar Switching Up-Down Relay Networks

  • Li, Jun;Lee, Moon-Ho;Yan, Yier;Peng, Bu Shi;Hwang, Gun-Joon
    • Journal of electromagnetic engineering and science
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    • v.11 no.4
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    • pp.239-249
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    • 2011
  • In this paper, we propose a reliable $8{\times}8$ up-down switching polar relay code based on 3GPP LTE standard, motivated by 3GPP LTE down link, which is 30 bps/Hz for $8{\times}8$ MIMO antennas, and by Arikan's channel polarization for the frequency selective fading (FSF) channels with the generator matrix $Q_8$. In this scheme, a polar encoder and OFDM modulator are implemented sequentially at both the source node and relay nodes, the time reversion and complex conjugation operations are separately implemented at each relay node, and the successive interference cancellation (SIC) decoder, together with the cyclic prefix (CP) removal, is performed at the destination node. Use of the scheme shows that decoding at the relay without any delay is not required, which results in a lower complexity. The numerical result shows that the system coded by polar codes has better performance than currently used designs.

Dynamic analysis of deployable structures using independent displacement modes based on Moore-Penrose generalized inverse matrix

  • Xiang, Ping;Wu, Minger;Zhou, Rui Q.
    • Structural Engineering and Mechanics
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    • v.54 no.6
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    • pp.1153-1174
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    • 2015
  • Deployable structures have gained more and more applications in space and civil structures, while it takes a large amount of computational resources to analyze this kind of multibody systems using common analysis methods. This paper presents a new approach for dynamic analysis of multibody systems consisting of both rigid bars and arbitrarily shaped rigid bodies. The bars and rigid bodies are connected through their nodes by ideal pin joints, which are usually fundamental components of deployable structures. Utilizing the Moore-Penrose generalized inverse matrix, equations of motion and constraint equations of the bars and rigid bodies are formulated with nodal Cartesian coordinates as unknowns. Based on the constraint equations, the nodal displacements are expressed as linear combination of the independent modes of the rigid body displacements, i.e., the null space orthogonal basis of the constraint matrix. The proposed method has less unknowns and a simple formulation compared with common multibody dynamic methods. An analysis program for the proposed method is developed, and its validity and efficiency are investigated by analyses of several representative numerical examples, where good accuracy and efficiency are demonstrated through comparison with commercial software package ADAMS.

Design and Development of m-Learning Service Based on 3G Cellular Phones

  • Chung, Kwang-Sik;Lee, Jeong-Eun
    • Journal of Information Processing Systems
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    • v.8 no.3
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    • pp.521-538
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    • 2012
  • As the knowledge society matures, not only distant, but also off-line universities are trying to provide learners with on-line educational contents. Particularly, high effectiveness of mobile devices for e-Learning has been demonstrated by the university sector, which uses distant learning that is based on blended learning. In this paper, we analyzed previous m-Learning scenarios and future technology prospects. Based on the proposed m-Learning scenario, we designed cellular phone-based educational contents and service structure, implemented m-Learning system, and analyzed m-Learning service satisfaction. The design principles of the m-Learning service are 1) to provide learners with m-Learning environment with both cellular phones and desktop computers; 2) to serve announcements, discussion boards, Q&A boards, course materials, and exercises on cellular phones and desktop computers; and 3) to serve learning activities like the reviewing of full lectures, discussions, and writing term papers using desktop computers and cellular phones. The m-Learning service was developed on a cellular phone that supports H.264 codex in 3G communication technology. Some of the functions of the m-Learning design principles are implemented in a 3G cellular phone. The contents of lectures are provided in the forms of video, text, audio, and video with text. One-way educational contents are complemented by exercises (quizzes).

An Empirical Analysis on Optimal Oder Quantity of Perishable and Seasonal Products : A Practical Application of Newsvendor Model in Retail (신선·시즌 상품의 최적 주문량 산정 문제에 대한 실증적 분석 : 소매유통업에서 뉴스벤더 모델의 적용)

  • Noh, Geon-Ho;Hwang, Seung-June
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.42 no.1
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    • pp.41-54
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    • 2019
  • Although retailers deals with a large number of single-term inventory items, but few cases have been considered in the areas of practical decision making. However, recent moves to strengthen fair trade have created a real need for single-period inventory decision-making problems. This study addresses the problem of ordering quantity decisions that are expected to maximize profits using classical newsvendor models. The research target is data on seasonal and perishable products from retail. We also use data from retailers to actually apply the newsvendor model and calculate the results to compare performance. It also suggests solutions for estimating demand for products sold in order to apply newsvendor models that utilize actual demand ratio versus forecast demand. This study would like to examine the effectiveness of this research through data analysis and make some suggestions for applying it to reality.

CDMA Digital Mobile Communications and Message Security

  • Rhee, Man-Young
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.6 no.4
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    • pp.3-38
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    • 1996
  • The mobile station shall convolutionally encode the data transmitted on the reverse traffic channel and the access channel prior to interleaving. Code symbols output from the convolutional encoder are repeated before being interleaved except the 9600 bps data rate. All the symbols are then interleaved, 64-ary orthogonal modulation, direct-sequence spreading, quadrature spreading, baseband filtering and QPSK transmission. The sync, paging, and forward traffic channel except the pilot channel in the forward CDMA channel are convolutionally encoded, block interleaved, spread with Walsh function at a fixed chip rate of 1.2288 Mcps to provide orthogonal channelization among all code channels. Following the spreading operation, the I and Q impulses are applied to respective baseband filters. After that, these impulses shall be transmitted by QPSK. Authentication in the CDMA system is the process for confirming the identity of the mobile station by exchanging information between a mobile station and the base station. The authentication scheme is to generate a 18-bit hash code from the 152-bit message length appended with 24-bit or 40-bit padding. Several techniques are proposed for the authentication data computation in this paper. To protect sensitive subscriber information, it shall be required enciphering ceratin fields of selected traffic channel signaling messages. The message encryption can be accomplished in two ways, i.e., external encryption and internal encryption.