• Title/Summary/Keyword: Pyruvic acid

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Dietary Nigella sativa and Peganum harmala Oils Reverses Hyperglycaemia, Hepatotoxicity, and Metabolism in Rats

  • Hamden, Khaled;Carreau, Serge;Jamoussi, Kamel;Ayadi, Fatma;Garmazi, Fadhel;Elfeki, Abdelfattah
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.739-744
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    • 2009
  • This study aims to evaluate the therapeutic action of administration of Nigella sativa (NS) and Peganum harmala (PH) oils in diabetes and hepatic toxicity. Results show that treatment of diabetic rats with NS oil or PH oil ameliorate hyperglycaemia induced stress oxidative and hepatic dysfunction in diabetic rats. Administration of NS or PH oil to diabetic rats caused an anti-diabetic and antioxidant activities by the decrease in plasmatic glucose level and increase in hepatic superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities, reduced glutathione (GSH) and glycogen contents compared to untreated diabetic rats. Besides, NS and PH oils protect the hepatic function observed by decrease of triglyceride (TG), total cholesterol (TCh), and increase of high density lipoprotein-cholesterol (HDL-Ch) levels in serum and hepatic tissues. Moreover, a diminution in the bilirubin, transaminase glutanic pyruvic (TGP), and transaminase pyruvic oxaloacetic (TPO) contents in serum and the thiobarbituric acid-reactive substances levels (TBARs) in hepatic tissues are also detected.

Effects of Applying Slow-Release Fertilizer on Southern Type Garlic (Allium sativum L.) Cultivation (난지형 마늘 재배 시 완효성 비료 시용효과)

  • Song, Yeon Sang;Choi, In Hu;Chung, Byeong Choon;Choi, Won yul
    • Horticultural Science & Technology
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    • v.19 no.4
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    • pp.471-475
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    • 2001
  • This experiment was conducted to evaluate the effect of slow-release fertilizer on warm type garlic cultivation in the Southwestern region of Korea. The slow-release fertilizers used were UF(Urea-Formaldehydes)70, 100, 130, CDU(Crotonylidene Diurea)100, and IBDU(Isobuty- lidene Diurea)100. The slow-release fertilizer treatment slightly increased plant height, leaf sheath diameter, and leaf number compare with conventional fertilizer treatment. Also, large bulb rate, nitrogen and pyruvic acid contents of garlic in slow-release fertilizer plot were higher than other treatments. Application of slow-release fertilizer increased the commercial yields.

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Quality Factors of Freshness and Palatability of Onion by Cultivation Region and Variety (재배지역과 품종에 따른 양파의 맛 관련 품질인자 설정)

  • Jeon, Hyeonjin;Kim, Byungsam;Lee, Sanghoon;Kim, Yoonsook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.6
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    • pp.859-867
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    • 2014
  • This study was conducted to investigate the relationship between quality factors and palatability of onion by cultivation region and variety. Six onion varieties harvested in four regions were subjected to physicochemical, microbiological, and sensorial evaluation. There were differences in soluble solid content, color, vitamin C, quercetin, total polyphenol, and pyruvic acid contents among the varieties and cultivation regions. Dry weight and pH of onions did not significantly differ. Overall acceptability and palatability did not show any significant differences by variety. From the correlation and regression analysis, palatability was very closely related to $Z_{11}$ (acridity) of -0.914 and the regression equation for sensorial palatability (Y) was $Y=12.3806-0.8284Z_{10}(sourness)-0.7957Z_{11}(acridity)$ with a coefficient of 0.9140.

Metabolic Pathways of Hydrogen Production in Fermentative Acidogenic Microflora

  • Zhang, Liguo;Li, Jianzheng;Ban, Qiaoying;He, Junguo;Jha, Ajay Kumar
    • Journal of Microbiology and Biotechnology
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    • v.22 no.5
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    • pp.668-673
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    • 2012
  • Biohydrogen production from organic wastewater by anaerobically activated sludge fermentation has already been extensively investigated, and it is known that hydrogen can be produced by glucose fermentation through three metabolic pathways, including the oxidative decarboxylation of pyruvic acid to acetyl-CoA, oxidation of NADH to $NAD^+$, and acetogenesis by hydrogen-producing acetogens. However, the exact or dominant pathways of hydrogen production in the anaerobically activated sludge fermentation process have not yet been identified. Thus, a continuous stirred-tank reactor (CSTR) was introduced and a specifically acclimated acidogenic fermentative microflora obtained under certain operation conditions. The hydrogen production activity and potential hydrogen-producing pathways in the acidogenic fermentative microflora were then investigated using batch cultures in Erlenmeyer flasks with a working volume of 500 ml. Based on an initial glucose concentration of 10 g/l, pH 6.0, and a biomass of 1.01 g/l of a mixed liquid volatile suspended solid (MLVSS), 247.7 ml of hydrogen was obtained after a 68 h cultivation period at $35{\pm}1^{\circ}C$. Further tests indicated that 69% of the hydrogen was produced from the oxidative decarboxylation of pyruvic acid, whereas the remaining 31% was from the oxidation of NADH to $NAD^+$. There were no hydrogen-producing acetogens or they were unable to work effectively in the anaerobically activated sludge with a hydraulic retention time (HRT) of less than 8 h.

Sterilization of Garlic Powder by Irradiation (방사선(放射線)에 의한 마늘분말(粉末)의 살균(殺菌))

  • Kwon, Joong-Ho;Byun, Myung-Woo;Cho, Han-Ok
    • Korean Journal of Food Science and Technology
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    • v.16 no.2
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    • pp.139-142
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    • 1984
  • Effects of irradiation on the microbial growth and physicochemical properties of garlic powder were investigated during 3 months storage. Total bacteria and coliform group of garlic powder were $4.74{\times}10^{4}$ and $5.0{\times}10^{3}$ per g, respectively and irradiation of 5 kGy and 7 kGy could sterilize coliform group and total bacteria, respectively. $D_{10}$ value of total bacteria was 3.34 and no microorganisms were grown in 5 to f kGy irradiated groups after 3 months storage at $30{\pm}1^{\circ}C$. Moisture, sugars and pH of garlic powder were not remarkably changed during storage but pyruvic acid content was slightly decreased with storage period. Color difference of garlic powder after 3 months storage could not be distinguished by naked eye, but a slight change was recongized by the mechanical measurement.

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Effect of Agitation on Production of Methylan and Rheological Characteristics of Methylan Fermentation Broth (다당류, 메틸란, 발효밴잉액의 점성특성과 메틸란 생산에 미치는 교반속도의 영향)

  • Oh, Deok-Kun;Lim, Hyun-Soo;Kim, Jung-Hoe
    • Applied Biological Chemistry
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    • v.38 no.3
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    • pp.191-195
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    • 1995
  • Production of a high viscosity exoploysaccharide, methylan, by Methylobacterium organophilum from methanol was carried out in fed-batch cultures and the rheological properties of methylan fermentation broth were studied. Bacterial biomass showed little influence on viscosity, but the accumulation of methylan caused the increase of viscosity. With proceeding fermention, the viscosity at the same concentration of methylan was significantly increased and methylan solution showed slightly higher pseudoplasticity. The composition changes of methylan were investigated at various fermentation times. Contents of total sugar, reducing sugar and methylan were decreased but contents of acids(pyruvic acid, uronic acid and acetic acid) were increased with the culture time. It was considered that the increased content of acids resulted in the increase of the hyrodynamic domain in the solution due to charge repulsion. Consequently, the solution viscosity increased in propotion to the acids contents of methylan. Cell growth and methylan production were severely decreased by the limitation of dissolved oxygen. However, the cellular activity for methylan production was almost constant regardless of the level of dissolved oxygen. As a result, the high speed of agitation increased the methylan production, the specific production rate of methylan, and the methylan yield of the cell.

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Chemical Component of Red Onion (Allium cepa L.) according to Cultivars and Growing Areas (품종, 지역에 따른 적색양파 화학성분)

  • Moon, Jin-Seong;Kim, Hee-Dae;Ha, In-Jong;Lee, Sun-Young;Lee, Jong-Tae;Lee, Sang-Dae
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.921-927
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    • 2010
  • The objective of this study was to improve the breeding efficiency and consumption of red onion by analysis of chemical components. The red cultivar S&P5294 ($52.9mg{\cdot}g^{-1}$ F.W.) resulted in the highest sugar content, and sweetness whereas 'Redprime' had the lowest. For the pyruvic acid content the cultivar 'Redsun' showed the highest value, followed by 'Sunpower' (yellow onion) and S&P7270. Breeding lines had higher sugar content and sweetness than 'Redprime' and 'Sunpower', while they had less pyruvic acid value than 'Redsun' and 'Sunpower'. The the same trend was verified for the total phenol and total flavonoid, of which breeding lines were found to have more content than 'Redprime' or 'Sunpower'. Three major flavonoids, rutin (quercetin glycoside), myricetin, and quercetin, were identified and quantified whereas kaempferol was not detected in all cultivars. Rutin was the major flavonoid present in all cultivars (136.9 to $169.9{\mu}g{\cdot}g^{-1}$ F.W.), followed by quercetin (62.5 to $135.7{\mu}g{\cdot}g^{-1}$ F.W.). Relatively smaller amount of myricetin (11.2 to $16.1{\mu}g{\cdot}g^{-1}$ F.W.) was also detected. Among nine cultivars, 'Redsun' exhibited significantly ($p$ < 0.05) higher content of quercetin and rutin (135.7 and $169.9{\mu}g{\cdot}g^{-1}$ F.W., respectively), and S&P5296 had the highest content of myricetin($16.1{\mu}g{\cdot}g^{-1}$ F.W.). Breeding lines had more quercetin, rutin and myricetin contents than the cultivars 'Redprime' and 'Sunpower'. Hampyeong had the highest content for total phenol and total flavonoid, followed by Andong and Changyeong whereas Changyeong had the highest value for rutin, myricetin and quercetin followed by Hampyeong and Andong. Among red onion cultivars, 'Redsun' showed the highest value for anthocyanin, followed by S&P5295 and S&P5296, which the cultivar 'Redprime' had significantly (p<0.05) lower content(35.5 mg%).

Chemical Characterization of Water-Soluble Organic Acids in Size-Segregated Particles at a Suburban Site in Saitama, Japan

  • Bao, Linfa;Sakamoto, Kazuhiko
    • Asian Journal of Atmospheric Environment
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    • v.3 no.1
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    • pp.42-51
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    • 2009
  • Saturated n-dicarboxylic acids ($C_2-C_7$, $C_9$), unsaturated dicarboxylic acids (maleic, fumaric, phthalic acid), ketocarboxylic acids (pyruvic, glyoxylic acid), and dicarbonyls (glyoxal, methylglyoxal) were determined in size-segregated samples with a high-volume Andersen air sampler at a suburban site in Saitama, Japan, May 12-17 and July 24-27, 2007 and January 22-31, 2008. The seasonal average concentrations of these detected organic acids were 670 $ng/m^3$, accounting for about 4.4-5.7% (C/C) of water-soluble organic carbon (WSOC) and 2.3-3.6% (C/C) of organic carbon (OC). The most abundant species of dicarboxylic acids was oxalic acid, followed by malonic, phthalic, or succinic acids. Glyoxylic acid and methyglyoxal were most abundant ketocarboxylic acid and dicarbonyl, respectively. Seasonal differences, size-segregated concentrations, and the correlations of these acids with ambient temperatures, oxidants, elemental carbon (EC), OC, WSOC, and ionic components were also discussed in terms of their corresponding sources and possible secondary formation pathways. The results suggested that photochemical reactions contributed more to the formation of particulate organic acids in Saitama suburban areas than did direct emissions from anthropogenic and natural sources. However, direct emissions of vehicles were also important sources of several organic acids in particles, such as phthalic and adipic acids, especially in winter.

Physicochemical Characteristics of Black Garlic after Different Thermal Processing Steps

  • Kang, Ok-Ju
    • Preventive Nutrition and Food Science
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    • v.21 no.4
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    • pp.348-354
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    • 2016
  • This study investigated the physicochemical characteristics of black garlic (BG) after different thermal processing steps. Compared with fresh garlic (FG), the moisture content and pH in BG decreased significantly, while the ash content and browning intensity increased during thermal processing. The total mineral and the free sugar contents were significantly higher than that of the BG2 and BG4 samples, respectively. The free sugar content increased by 16-fold in the BG cloves compared with that of FG, while the amino acid content increased during the first stage of thermal processing, and subsequently decreased. The thiosulfinate content in all samples decreased to during thermal processing. The pyruvic acid content initially increased and then decreased during thermal processing. These results contribute to our understanding of the role of thermal processing in the quality formation of BG.

Hepatoprotective Effect of Extracellular Polymer Produced by Submerged Culture of Ganoderma lucidum WK-003

  • Song, Chi-Hyun;Yang, Byung-Keun;Ra, Kyung-Soo;Shon, Dong-Hwan;Park, Eun-Jeon;Go, Geon-Il;Kim, Young-Hwoan
    • Journal of Microbiology and Biotechnology
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    • v.8 no.3
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    • pp.277-279
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    • 1998
  • An extracellular polymer (exo-polymer) with hepatoprotective properties was produced after a 6-day submerged mycelial culture of Ganoderma lucidum WK-003. The glutamic pyruvic transaminase (GPT) activities in the serum of intoxicated Sprague-Dawley rats were decreased from 871 to 263 by the oral administration of the exo-polymer (20 mg/kg/day) for 4 consecutive days. Rhamnose, arabinose, xylose, mannose, galactose, and glucose were found in the exo-polymer along with aspartic acid, glutamic acid, histidine, serine, glycine, arginine, alanine, tryptophan, valine, phenylalanine, isoleucine, and leucine.

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