• Research in Community and Public Health Nursing
• /
• v.10 no.2
• /
• pp.455-466
• /
• 1999

Western missionary nurses practiced in Korea from 1891. and the first trial to begin missionary public health nursing service in 1909 could not put into practice for short of nursing staff and budget. The main focus of missionary medical practice was not in public health program but in the management of missionary hospitals. A few of missionary western R.N. tried district nursing in 1910s. but their activities were personal and focused on the rescue of poor and sick patients. In 1917 the North American Methodist Church dispatched R.N. Elizabeth S. Roberts to begin district nursing in Korea. Roberts began maternal and child district nursing service. Her service was focused on teaching the method of bringing up children. bathing service, and home visiting for delivery. She could not but stop district-nursing service in 1918 to serve for a hospital in Siberia. The North American Methodist Church dispatched a few of R.N. to Korea in early 1920s and the missionary public health nursing of Korea could be activated. R.N. E. T. Rosenberger began public health nursing program in Seoul with Korean graduate nurse, Shin-gwang Han, and missionary M.D. Hall. Their public health nursing program was focused on maternal and childcare. They did home visiting in the morning, and served at a well baby clinic in the afternoon. The first baby competition began in 1925. and contributed to the teaching the method of bringing up children. They expanded public health nursing activity to school health nursing and milk station. Their public health nursing program was such a success that In 1929 Severance hospital. Eastgate Hospital. Taehwa Social Evangelistic center organized Seoul Child Health Union. Maren P. Bording, another missionary R.N. and midwife dispatched by the North American Methodist Church began public health nursing program at Kongjoo in 1924. Her program was focused on the maternal and childcare and close to that of Seoul. She started the first milk station in Korea in 1926. As she was a midwife and could get M. D. license in Korea, her program was more focused on maternal care than that of Seoul. The first day nursery school in Korea and the first graduate course for public health nursing in Korea began at Kongjoo in 1930. As the city of Choongcheongnam Province moved from Kongjoo to Daejeon in 1932, missionary public health nursing service in Kongjoo extended to Daejeon. There were lots of public health nursing program in Korea in 1920s and 1930s by missionary western nurses and Korean nurses. There were 13 missionary public health-nursing center in Korea in 1932. But in the late 1930s. Japan extended colonial war and drove out western missionaries. The missionary service in Korea was daunted. and the missionary public health nursing service could not but shrink.

• Korean Journal of Organic Agriculture
• /
• v.23 no.4
• /
• pp.847-858
• /
• 2015

Bacillus amyloliquefaciens GR4-5 was isolated from the rhizosphere soil of Korean ginseng and displayed broad-spectrum suppression of ginseng root rot pathogens. The survivability of B. amyloliquefaciens GR4-5 in soil was investigated under three different conditions; indoor, outdoor - of which soil was put in 14 mL tube after treatment - and field environments. Soil samples were collected over a four-week period from three experimental designs, and assessed for 16S rRNA gene copy number by quantitative polymerase chain reaction (qPCR). In outdoor condition, the 16S rRNA gene copy number of Bacillus spp. was 8.35 log copies g $soil^{-1}$ immediately after the GR4-5 treatment. Two weeks later, the 16S rRNA gene copy number of Bacillus spp. (6.70 log copies g $soil^{-1}$) was similar to that of the control (6.38 log copies g $soil^{-1}$). In indoor condition, the 16S rRNA gene copy number of Bacillus spp. maintained in a certain level for a longer period than those in outdoor and field. The 16S rRNA gene copy number of Bacillus spp. in field experiment was reduced faster than that of outdoor condition. Our results show that B. amyloliquefaciens GR4-5 can survive in bulk soil for 1 week, indicating its potential use as a biocontrol agent following 7 day application intervals. This study presents that outdoor microcosm system design could be a useful method to assess easily the survivability of beneficial microorganisms.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.33 no.6
• /
• pp.1034-1042
• /
• 2004

This study was carried out to investigate preparation of reconstructed red peppers, effects of pungency and redness of red peppers on the Kimchi quality using central composite design and response surfaces methodology. Capsaicinoids and ASTA (American Spice Trading Association) value put in X$_1$, X$_2$ of independent variable. The result of response surface regression analysis of reconstructed red peppers, correlation coefficient ($R^2$) of overall pungency intensity, persistence and degree of redness was 0.935, 0.935 and 0.821, respectively. After it was made Kimchi samples with reconstructed red peppers, it was examined pH, titratable acidity and lactic acid bacteria of its during fermentation. In the initial fermentation period of Kimchi, it showed pH of 5.46∼5.78, titratable acidity of 0.27∼0.31%, salt content of 2.26∼2.48% and lactic acid bacteria of 4.05${\times}$10$^{5}$ ∼6.23${\times}$10$^{5}$ , respectively. And it showed traditional fermentation pattern in the pH, titratable acidity and microbes of the middle (appropriate fermentation) and last (excessive) fermentation period. While capsaicinoids content in the Kimchi decreased a little according to extend fermentation period, ASTA value showed low correlation reconstructed red pepper and fermentation period. Also, it was analyzed correlation coefficient ($R^2$) of independent variables (capsaicinoids, X$_1$; ASTA value, X$_2$) between sensory attribute in the Kimchi during fermentation. The result of regression analysis, $R^2$ in the overall pungency intensity, persistence and degree of redness showed 0.515, 0.675, 0.784, respectively.

• Tunnel and Underground Space
• /
• v.33 no.5
• /
• pp.388-398
• /
• 2023

The changes in groundwater flow due to mining development act as a contributing factor to major issues such as ground subsidence, strength reduction and collapse. For the sustainable mining development, measures for dealing with fluctuations in seasonal underground water inflow, power losses, pump damage, and unexpected increases in inflow must be put in place. In this study, the aim is to identify the causes of underground seepage through the examination of hydrological connectivity between the study area and nearby limestone mine. A tracer tes for assessing subsurface connectivity has been planned. A variety of tracers, such as dyes and ions, were applied in lab test to select the optimal tracer material, and a hydrological model of the study area was implemented through field test. Finally, the hydrological connectivity between the external stream and underground water in the mine was analyzed.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.3
• /
• pp.552-559
• /
• 2008

Ethyl (S)-4-chloro-3-hydroxybutyrate is an intermediate for the synthesis of Atorvastatin, a chiral drug used for hypercholesterolemia. A Rhodococcus erythropolisstrain (No.7) able to convert 4-chloro-3-hydroxybutyronitrile into 4-chloro-3-hydroxybutyric acid has recently been isolated from soil. This activity has been regarded as having been caused by the successive actions of the nitrile hydratase and amidase. In this instance, the corresponding amidase gene was cloned from the R. erythropolis strain and expressed in Escherichia coli cells. A soluble active form of amidase enzyme was obtained at $18^{\circ}C$. The Ni column-purified recombinant amidase was found to have a specific activity of 3.89 U/mg toward the substrate isobutyramide. The amidase was found to exhibit a higher degree of activity when used with mid-chain substrates than with short-chain ones. Put differently, amongst the various amides tested, isobutyramide and butyramide were found to be hydrolyzed the most rapidly. In addition to amidase activity, the enzyme was found to exhibit acyltransferase activity when hydroxyl amine was present. This dual activity has also been observed in other enzymes belonging to the same amidase group (E.C. 3.5.1.4). Moreover, the purified enzyme was proven to be able to enantioselectively hydrolyze 4-chloro-3-hydroxybutyramide into the corresponding acid. The e.e. value was measured to be 52% when the conversion yield was 57%. Although this e.e. value is low for direct commercial use, molecular evolution could eventually result in this amidase being used as a biocatalyst for the production of ethyl (S)-4-chloro-3-hydroxybutyrate.

• The Bulletin of The Korean Astronomical Society
• /
• v.40 no.1
• /
• pp.61.1-61.1
• /
• 2015

We performed a monitoring campaign of a Jupiter-Family comet 17P/Holmes, which underwent the dramatic outburst on 23.3 October 2007 at $r_h=2.44AU$, to investigate the secular change in activity and subsequent physical properties of the inner dust coma before and after the 2014 perihelion passage. The monitoring observation was carried out over two years: from May to July 2013, from July to November 2014, and January 2015 with ~weekly cadence. We conducted photometry monitoring in Rc band using four ground-based telescopes, which are the Ishigakijima Astronomical Observatory 105cm telescope, the Okayama Astrophysical Observatory 50cm telescope, the Nishi-Harima Astronomical Observatory 2m telescope, and the T30 51cm i-telescope, respectively. In order to examine the dust production rate, we put a constraint upon the physical distance from the center of the nucleus as rho=2500km and conducted aperture photometry. We found that the average absolute Rc magnitude over the period between July to November 2014 was mR(1,1,0)~12.29, which was approximately 1.5 magnitudes fainter than those of 2013 data. Accordingly, comet 17P/Holmes seemed to become dormant, although a minor eruption was detected on January 26, 2015. In this presentation, we will introduce our ongoing project for 17P/Holmes and discuss why the nucleus becomes dormant within one orbital period.

• Journal of Microbiology and Biotechnology
• /
• v.11 no.1
• /
• pp.21-28
• /
• 2001

Enzyme linked-immunosorbent assay (ELISA) for a rapid detection of fungi, Aspergillus and Penicillium genera in food, were developed and their efficiencies were approved by detecting artificially contaminated agricultural commodities. Mice were immunized with partially purified Aspergillus flavus extracellualr polysaccharide (EPS) and lymph node cells of the mice were fused with the myeloma cells for production of monoclonal antibodies. Mab 1G11, one of the antibodies, was selected and purified. A sandwich ELISA was established and its detection limit toward A. flavus EPS was 1mg/ml. Among the 59 strains tested (including 18 species of Aspergillus, 16 of Penicillium, 11 of Fusarium, 1 of Absidia, 2 of Alternaria, 2 of Candida, 2 of Cladosporium, 2 of Geotrichum, 2 of Mucor, 2 of Rhizopus, 1 of Trichoderma), species of Aspergillus and penicillium had a high reactivity with Mab 1G11 even up to 10,000 times dilution of culture broths. The other genera except Cladosporium resinae showed no reactivity, thus Mab 1G11 was specific to the genera of Aspergillus and Penicillium. The epitope of A. flavus EPS against monoclonal Mab 1G11 was on the carbohydrate moiety when 1 to 100$\mu g/g$ A. flavus EPS were put into rice, potato, and mandarin orange, the average recoveries detected by sandwich ELIA were 123, 59, and 76%, respectively. Correlation was found to be linear between the EPS, and mycelium of A. flavus and Penicillium citrinum grown in a liquid medium (r=0.87 and 0.96), and also between the EPS and colony forming unit in solid media of rice of potato (r=0.91-0.99).

• Journal of Biosystems Engineering
• /
• v.22 no.3
• /
• pp.295-302
• /
• 1997

An ultimate purpose of this study was to develop an automatic system for brown rice quality inspection using image processing technique. In this study emphasis was put on developing an algorithm for discriminating the brown rice kernels depending on their external quality with a color image processing system equipped with an adaptor magnifying the input image and optical fiber for oblique lightening. Primarily, geometical and optical features of images were analyzed with paddy and the various brown rice kernel samples such as a sound, cracked, peen-transparent, green-opaque, colored, white-opaque and brokens. Secondary, geometrical and optical parameters significant for identifying each rice kernels were screened by a statistical analysis(STEPWISE and DISCRIM procedure, SAS wer. 6) and an algorithm fur on- line discrimination of the rice kernels in static state were developed, and finally its performance was evaluated. The results are summarized as follows. 1) It was ascertained that the cracked kernels can be detected when e incident angle of the oblique light is less than 2$0^{\circ}C$ but detectivity was significantly affected by the angle between the direction of the oblique light and the longitudinal axis of the rice kernel and also by the location of the embryo with respect to the oblique light. 2) The most significant Parameters which can discriminate brown rice kernels are area, length and R, B and r values among the several geometrical and optical parameters. 3) Discrimination accuracies of the algorithm were ranged from 90% to 96% for a sound, cracked, colored, broken and unhulled, about 81 % for green-transparent and white-opaque and 75 % for green-opaque, respectively.

• Journal of the Korean association of regional geographers
• /
• v.12 no.6
• /
• pp.704-719
• /
• 2006

The study deals with the France's recent cluster policy represented by the Competitiveness Pole. As a national level cluster supporting policy driven by the central government, the Competitiveness Pole, called 'French cluster model', :is competitiveness-oriented, and principally based on three main factors: partnerships, R&D projects and international visibility. The Competitiveness Pole is also the fruit or a long time effort to establish a decentralized governance system, and it has a lot for Korea to benchmark its relevant policies. After reviewing the Local Production System that was put in place before the Competitiveness Pole, the study treats main aspects or Competitiveness Pole policy. It analyze, then, the significance or the policy in the trend or the overall territorial policy or the country. Lastly, the study provides some implications for Korean policies such as industrial clusters, the Enterprise City and Innovation City.

• KDI Journal of Economic Policy
• /
• v.35 no.4
• /
• pp.1-29
• /
• 2013

This paper examines the effect of trade liberalization or globalization, more broadly, on plants' growth as well as on "bi-polarization". To do so, we reviewed the possible theoretical mechanisms put forward by recent heterogeneous firm trade theories, and provided available micro-evidence from existing empirical studies on Korean manufacturing sector. Above all, the empirical evidence provided in this paper strongly suggests that globalization promoted growth of Korean manufacturing plants. Specifically, evidence suggests that exporting not only increases within-plant productivity but also promotes introduction of new products and dropping of old products. However, the empirical evidence also suggest that globalization has some downsides: widening productivity differences across plants and rising wage inequality between skilled and unskilled workers. Specifically, trade liberalization widens the initial productivity differences among plants through learning from export market participation as well as through interactions between exporting and R&D, both of which increase plants' productivity. We also show that there is only a small group of large and productive "superstar" plants engaged in both R&D and exporting activity, which can fully utilize the potential benefits from globalization. Finally, we also show evidence that trade liberalization interacts with innovation to increase the skilled-unskilled wage inequality.