• 상하수도학회지
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• 제33권5호
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• pp.329-339
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• 2019

Recently, the advancement of information and communication technology(ICT) is expanding the connectivity through Internet of Things(IoT), and the media of connection is also expanding from wire/cable transmission to broadband wireless communication, which has significantly improved mobility. This hyperconnectivity has become a key element of the fourth industrial revolution, whereas the supervisory control network of purification plants in korea is operated as a communication network separated from the outside, thereby lagging in terms of connectivity. This is considered the best way to ensure security, and thus there is hardly any consideration of establishing alternatives to operate an efficient and stable communication network. Moreover, security for management of a commercialized communication network and network management solution may be accompanied by immense costs, making it more difficult to make new attempts. Therefore, to improve the conditions for the current supervisory control network of purification plants, this study developed a industrial security L2 switch that supports modbus TCP(Transmission Control Protocol) communication and encryption function of the transmission section. As a result, the communication security performance improved significantly, and the cost for implementing the network management system using Historical Trend and information of HMI(Human Machine Interface) could be reduced by approximately KRW 200 million. The results of this study may be applied to systems for gas, electricity and social safety nets that are infrastructure communication networks that are similar to purification plants.

• KSBB Journal
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• 제30권3호
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• pp.103-108
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• 2015

Production of foreign proteins by transgenic plant cell cultures has several advantages such as post-translational modification, low risk of product contamination and low-cost production and purification. However, target proteins are degraded by extracellular proteases existing in the media. A solution to this problem is the use of perfusion culture and ion exchange chromatography for the application of integrated bioprocess using in situ recovery. With this method, production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in this study. First, optimization of cell concentration during the induction phase for the production of hGM-CSF was examined. As cell concentration increased, the level of hGM-CSF was decreased due to the presence of extracellular proteases. Induction using sugarfree media produced 33% more hGM-CSF. The effects of pH on the binding of hGM-CSF to cationic and anionic exchange resins were also investigated. In terms of stability, optimal pH was found to be 5~7. In the case of using buffer exchange when CM-Sepharose was used as a cationic exchange resin, optimal pH for binding was 4.8 and adsorption yield was 77%. When DEAE-Sepharose was used as an anionic exchange resin, it was 5.5 (74%). Without buffer exchange, optimal pH was 4.6 (84%). From these results, an integrated bioprocess using in situ recovery with simultaneous production and separation of foreign protein in transgenic plant cell suspension cultures was found to be feasible.

• 한국환경복원기술학회지
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• 제13권6호
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• pp.145-159
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• 2010

A Sustainable Structured wetland Biotop (SSB) system was planned, designed, and finally constructed, and maintained in the An-teo Reservoir ecological park, which is the habitat of the endangered Gold-spotted Pond Frog. The system purifies polluted water of An-teo Reservoir which flows from up to bottom within the system. Water was sampled once a month at the inlet and at the outlet from December, 2009 to August, 2010. BOD5, SS, T-N and T-P were analyzed. Average influent and effluent BOD5 concentration was 2.9 and 1.0 mg/L, respectively, and BOD5 removal was 67%. SS concentration of influent and effluent averaged 18.1 mg/L and 2.5 mg/L, respectively, and SS abatement amounted to 86%. Average influent and effluent T-N concentration was 0.426 mg/L and 0.147 mg/L, respectively, and T-N retention was 66%. T-P concentration of influent and effluent averaged 0.071 mg/L and 0.022 mg/L, respectively, and T-P removal amounted to 68%. Plant and frog species of the system were monitored during the period. Amphibia and reptiles provided 7 species and 4 families including the Endangered Gold-Spotted Pond Frog (Rana chosenica ) which also lives in the system. Twenty-six plant species were naturally introduced into the system, however, they didn't make up a significant portion of the plant populations compared with the planted species. The endangered plants, Bladderwort (Utricularia vulgaris var. japonica ) and Euryale ferox were observed in An-teo Reservoir as well as in the system.

• The Plant Pathology Journal
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• 제26권3호
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• pp.280-285
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• 2010

In the course of a searching natural antifungal compounds from plant sources, we found that the methanol extract ($3,000\;{\mu}g/ml$) of Malus domestica fruits had potential of control against rice blast (Magnaporthe grisea) and tomato late blight (Phytophthora infestans). Under bioassay-guided purification, we isolated phloretin, a phenolic compound, with in vivo antifungal activity against M. grisea. By 1-day protective application of phloretin ($500\;{\mu}g/ml$), the compound strongly inhibited the disease development of M. grisea and P. infestans on rice and tomato seedlings, respectively. And red pepper anthracnose caused by Colletotrichum coccodes also was moderately suppressed. However, rice sheath blight (Rhizoctonia solani AG1), and barley powdery mildew (Blumeria graminis f. sp. hordei) were hardly controlled. In addition, the compound showed in vitro antifungal activity against some plant pathogenic fungi including Phytophthora capsici, Alternaria panax, Sclerotinia sclerotiorum, R. solani AG4, and M. grisea. This is the first report on the antifungal activity of phloretin against plant pathogenic fungi.

• 한국환경복원기술학회지
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• 제8권5호
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• pp.1-9
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• 2005

This study was conducted to investigate the effect of aquatic plant as a botanical air purification on the indoor air pollution by formaldehyde. Three aquatic plants such as Eichhornia crassipes, Cyperus alternifolius, Echinodorus cordifolius, were selected for this study and they were placed in the artificially contaminated chamber under laboratory condition. The results showed that all three plants could remove the formaldehyde from the contaminated air system effectively. Reduction in the formaldehyde levels by Eichhornia crassipes, which is the floating plant, might be associated with the factors of plant and water. Reduction in the formaldehyde levels by Cyperus helferi and Echinodorus cordifolius, which were emergent plant, was due to the complex effect of plant, soil medium and water. In aquatic plant system, dissolution, microbial degradation in rhizosphere, uptake through root and shoot, sorption to soil and shoot, hydrolysis are known as the main mechanisms of water soluble pollutants in the given system. The advantages of indoor air quality control system using aquatic plants can be; 1) various purifying mechanisms than foliage plants, 2) effective for decontamination of water soluble pollutants; 3) easy for maintenance; 4) diverse application potential. Therefore it was suggested from the results that indoor air control system of aquatic plants should be more effective for reduction of indoor air pollutants.

• 생약학회지
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• 제28권4호
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• pp.179-184
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• 1997

To search for useful antioxidants from plant materials, we investigated the antioxidative activity in the methanol extracts of 30 Chinese medicinal plants using DPPH method. The highest activity $(RC_{50}:\;12\;{\mu}g)$ was showed in the methanol extract of Rosa rugosa, followed by Potentilla fruticosa $(14\;{\mu}g)$, P. fragarioides $(16\;{\mu}g)$. And Geum aleppicum $(18\;{\mu}g)$. From the leaves of R. rugosa, two antioxidative compounds were isolated by a bioassay guided purification and identified as isoquercitrin and ${\beta}-glucogallin$ on the basis of $^1H-,\;^{13}C-\;NMR$, and FAB-MS data. ${\beta}-Glucogallin$ is the first report in this plant. The DPPH radical scavenging activity of ${\beta}-glucogallin\;(RC_{50}:\;8.5\;{\mu}g)$ was more effective than those of ${\alpha}-tocopherol\;(12\;{\mu}g)$ and BHA $(14\;{\mu}g)$.

• 인간식물환경학회지
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• 제24권2호
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• pp.179-194
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• 2021

Background and objective: Plant-based biofilter system has become a promising candidate for improving indoor air quality due to its eco-friendly and sustainable nature. However, it remains controversial whether indoor air purification using a biofilter is effective. Methods: This study used the one-group pre- and post-test design to analyze the effects of the operation of the plant-based biofilter system on indoor air quality, students' perception of indoor air quality, health effects, and subjective and objective attentiveness. Results: Results of this study show that the application of the plant-based biofilter system changed the air environment in the classroom. The operation of the plant-based biofilter system significantly decreased PM_2.5 and PM₁₀ and slightly increased temperature and humidity. Satisfaction with air quality and subjective attentiveness were significantly improved but objective attentiveness and health effects were not improved. Conclusion: Further studies need to be conducted for improving indoor air quality of schools and evaluating the health effects and attention of occupants to present the objective performance of the plant-based biofilter system.

• Journal of Plant Biotechnology
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• 제4권3호
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• pp.95-101
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• 2002

Molecular faming has the potential to provide large amounts of recombinant protein for use in diagnostics and as therapeutics. Various strategies have been developed to enhance the expression level, stability, and native folding of recombinant proteins produced in plants. Few investigations into the subcellular distribution of recombinant proteins within plant cells have been published despite the potential to increase the expression level and impact the purification process. This review article discusses the current strategies used for targeting recombinant proteins to various subcellular locations and the advantages of targeting to seed oil bodies for molecular farming applications. Specifically, the affinity capture of antibodies using recombinant oilbodies is discussed.

• The Plant Pathology Journal
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• 제25권1호
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• pp.103-107
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• 2009

In the course of a searching natural antifungal compounds from plant seeds, we found that the methanol extract of Psoralea corylifolia seeds showed potent control efficacy against tomato late blight caused by Phytophthora infestans and wheat leaf rust Puccinia recondita. Under bioassay-guided purification, we isolated two furanocoumarins, psoralen and isopsoralen, with anti-oomycete activity against P. infestans. By 1-day protective application, both compounds strongly reduced the disease development of P. infestans on tomato seedlings, but hardly controlled development of leaf rust on wheat seedlings. This is the first report on the anti-oomycete activity of P. corylifolia as well as that of psoralen and isopsoralen.

• Journal of Plant Biology
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• 제32권4호
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• pp.275-283
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• 1989

$\beta$-1, 4-endoglucanase (EC 3, 2, 1, 4) was isolated and purified from Nicotiana tabaccum L. Var. Virginia 115 suspensin cultured cells. The molecular weight as estimated by Sephadex G-100 was about 14, 000. The optimum pH for activity was 5.4. The Km value for carboxymethyl cellulose was 0.18 mg/unit and the enzyme was quite resistant to heating. Polyamine did not affect the activity of $\beta$-1, 4-endoglucanase in vitro but the activity increased drastically when polyamines were added in the suspension culture medium. It is suggested that increase in $\beta$-1, 4-endoglucenase activity due to polyamine might be related to growth of the plant.