• 한국지역지리학회지
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• 제10권4호
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• pp.757-774
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• 2004

이 논문은 전통적인 하천의 기능을 보다 체계적으로 재분류하여, 사회적 측면(용수공급, 관리, 교통 및 에너지원), 공간적 측면(지형형성, 수변경관, 지역구성, 경계-분리) 그리고 생태적 측면(폐수배출, 정화작용, 서식지, 기후조절) 등으로 구분하고자 한다. 이러한 재분류에 기초하여 보면, 근대화 과정에서 도시하천의 기능들 가운데 특히 용수공급 등 사회경제적 기능이 강조된 반면, 공간적 측면과 생태적 측면은 무시되었다는 점이 지적될 수 있다. 대구 및 그 주변 지역을 관류하는 금호강 역시 과거 지역사회의 내적 발전과정에 지대한 기여를 했지만, 하천의 특정 기능이 선별적으로 개발된 결과 금호강은 유지수의 부족과 더불어 오염과 퇴락으로 그 본래 기능을 상실하게 되었다. 뿐만 아니라 이로 인해 금호강에 의존하여 발달해 오던 대구 및 그 주변 도시들도 더 이상 성장하기 어렵게 되었다. 이러한 사회 환경적 위기 상황을 극복하기 위하여, 본 논문은 도시 하천이 가지는 고유한 기능들에 근거하여 지속가능성 원칙과 주요 기능별 평가 지표를 개발하고, 도시와 하천이 공생적으로 발전할 수 있는 방안들을 제시하고자 한다.

• 한국작물학회지
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• 제41권spc1호
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• pp.110-122
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• 1996

The n-3 family fatty acids containing ${\alpha}$-linolenic acid(18:3, ALA) have been known as physiological activation materials such as inhibitory effects on the incidence of hyper-tension, coronary heart disease and cancers as well as the control of senilc dementia. Although a lot of ALA(about $63\%$) are contained in perilla oil, it has not been commercialized yet because the purification technique of the ALA has not been well established. The procedure of purification of ALA from perilla oil was saponified with 1 N-KOH /ethanol and then saturated and low level unsaturated fatty acids were removed by low-temperature crystallization method. The concentrated unsaturated fatty acids (containing about $75\%$ ALA) went down through the silver nitrate-impregnated silica column chromatography for separation of high purity of ALA. The results obtained we Fraction B, C and D contained ALA more than $85.5\%$(recovery, >$88.9\%,\;95.4\%$(recovery, >$54.4\%$) and $99.9\%$(recovery, >$31.5\%$) in purity, respectively. Seed oil content of the tested varieties were ranged from 34.8 to $54.1\%$ with $45.3\%$ of varietal means. The major omega fatty acids contained in the oil were oleic acid(n-9) $15.2\%$, linoleic acid(n-6) $13.9\%$ and linolenic acid(n-3) $63.1\%$ in the mean value. Varietal variation of n-9, 6 and 3 fatty acids ranged of $9.5\~21.4\%,\;9.1\~20.4\%$ and $50.6\~70.5\%$ respectively. Unsaturated fatty acid were averaged $92.2\%$ of seed oil in fatty acid composition. The ratios of n-6 to n-3 ranged of $0.13\~0.34\%$($0.22\%$ in mean value). The highest n-3 fatty acid variety was Yecheonjong being $70.5\%$. The lowest variety in ratios of n-6 to n-3 was Goseongjong being $0.13\%$. Oil content showed positive correlation with stearic acid and linolenic acid, while the negative correlation with oil content and linoleic acid. On the other hand, A significant negative correlation were showed between linolnic acid and the ratios n-6/n-3 fatty acid, saturated fatty acid. Saturated fatty acid was highly correlated with unsaturated fatty acid negatively being $r= -0.723^{**}$.

• 한국해양학회지:바다
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• 제10권1호
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• pp.56-68
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• 2005

강화도 남단 갯벌의 상부조간대 세 정점에서 퇴적물 시료를 채취하여, 공극수에서 질산염 등 영양염을 분석하였다. 질산염의 공극수내 분포를 단순한 1차원 모델로 분석한 결과, 탈질산화율은 장화리에서 $7.8{\sim}9.4{\times}10^{-7}{\mu}mol{\cdot}cm^{-2}{\cdot}sec^{-1}$, 동막에서 $1.4{\sim}3.6{\times}10^{-7}{\mu}mol{\cdot}cm^{-2}{\cdot}sec^{-1}$로 추정되었다. 이는 타 지역에서 보고된 반응속도와 별 차이가 나지 않는 규모였다. 탈질산화율은 여름철에 낮았으며, 퇴적물 입도가 상대적으로 조립한 장소에서 1.5배 이상 빠르게 나타나서, 입도가 탈질산화 반응물질의 공급속도를 조절하는 중요한 인자 중의 하나로 판단되었다. 탈질산화는 무기질소 성분을 $N_2$로 영구적으로 갯벌의 계 외로 제거함으로써 지화학적 정화능으로 제시될 수 있지만, 열역학적 판단기준으로 보면 계에 대해 정화라는 개념을 부여하는 데에는 문제가 있다고 판단된다. 또한 현재 갯벌이란 용어가 생태환경적 기능이 상이한 염습지, 모래갯벌과 펄갯벌을 통칭하고 있어서 과학적인 분류가 시급한 것으로 나타났다.

• 한국수정란이식학회지
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• 제24권4호
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• pp.259-263
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• 2009

Salivary lipocalin (SAL1) is a member of the lipocalin protein family that has a property to associate with many lipophilic molecules and was identified as pheromone-binding protein in pigs. Our previous study has shown that SAL1 is expressed in the uterine endometrium in a cell type- and implantation stage-specific manner and secreted into the uterine lumen in pigs. However, function of SAL1 in the uterus during pregnancy in pigs is still not known. To understand physiological function of SAL1 in the uterine endometrium during pregnancy in pigs, it needs to elucidate the ligand(s) for SAL1. Thus, to identify the ligand for SAL1 in the porcine uterus, we collected uterine luminal fluid from pigs on day 12 of pregnancy by flushing with PBS. Proteins from the uterine luminal fluid were separated by ion exchange chromatography and gel filtration. Fractions containing SAL1 protein were pooled and concentrated. Immunoblot analysis confirmed successful purification of SAL1. Then, we extracted lipids from the purified SAL1 protein and analyzed the lipids by liquid chromatography-mass spectrometry, and predicted to be steroid hormones and prostaglandins as SAL1 ligands. Results in this study showed that SAL1 protein in the uterine secretions has a small lipophilic molecule as a natural ligand. Further characterization of ligand extracted from purified SAL1 will be useful for understanding physiological function of SAL1 during pregnancy and its application to increase the pregnancy rate in pigs.

• 동의생리병리학회지
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• 제21권4호
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• pp.822-834
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• 2007

This study searched the philosophical and medical thought of mutual understanding and human body. Mutual understanding is a fundamental problem in all branches of oriental studies. In other words, mutual understanding becomes the existential foundation of heaven-earth-human(三才). So human beings, heaven and the earth can't exist if there is no mutual understanding. It comes out the problem of self-consciousness in philosophy, and qi movement pattern like upward, downward, inward and outward movement in traditional korean medicine. Human beings have mutual understandings with heaven and the earth from a macroscopic standpoint, on the other side the human body from a microscopic standpoint. Qi movement is the mutual understanding and response of qi in human body, so with which the physiological functions and pathologic changes of viscera and bowels comes out. Therefore we want to present how to complete qi movement between viscera-bowels and organs to examine mutual understandings in human body closely. The results was summarized as follows; First, upbearing the clear yang and down bearing the turbid yin of spleen-stomach is main pivot of upward and downward of qi movement, and it is true form of mutual understandings between viscera and bowels, so upward, downward, inward and outward movement of whole viscera and bowels can be controlled by spleen-stomach. Second, by restraining relationships between downward heart fire and upward kidney water, heart fire and kidney water have close communications physiologically and pathologically as upper-lower, yin-yang and water-fire. Third, by restraining relationships between upbearing and effusion of liver and purification and down-sending of lung, liver and lung are outer circles of upward and downward movement, so they have antagonistic functions. Firth, by the relationships between upbearing the clear and down bearing the turbid of spleen-stomach and free coursing of liver, free coursing of liver-gallbladder is the essential requirement that it certify transportation and transformation of spleen-earth, so the disease of liver-gallbladder induce spleen-stomach disease pattern to affect its function of transportation and transformation. Fifth, by spleen and kidney, spleen yang is based on kidney yang, so the weakness of kidney yang can affect the function of transportation and transformation of spleen-stomach as it can't warm spleen earth. Sixth, by homogeny of liver-kidney, essence and blood of liver-kidney and ministerial fire of liver-kidney have mutal generation and limitation physiologically and they mutually are affected in pathologically.

• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1583-1590
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• 2021

Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti-PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.

• 한국지리정보학회지
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• 제14권4호
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• pp.102-115
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• 2011

본 연구에서는 지속가능한 산림녹지 계획을 위해 체계적인 지표선정 과정을 통한 평가모형을 구축하고, GIS 기법을 이용하여 대구광역시의 산림녹지에 대한 환경조절적 기능을 평가하였다. 평가지표는 문헌조사를 통한 35개의 기초지표를 선정하였으며, 브레인스토밍 과정을 통해 29개 지표로 축약하였다. 또한, 1차 설문으로 8개의 환경조절적 지표를 선정하였고, 2차 설문에 의한 MCB 분석 과정을 통한 탄소저감, 기온저감, 바람생성, 물순환성, 대기정화의 최종지표를 선정하였다. 평가모형은 AHP 분석에 의해 지표별 가중치를 평가하여 이를 기반으로 구축하였다. 산림 녹지에 대한 환경조절적 기능을 평가한 결과, 앞산, 비슬산, 팔공산 등 산림녹지의 정상부 및 주변지역에서 66점 이상으로 높은 점수가 나타났다. 반면, 와룡산 및 북구 칠곡지구 주변의 산림녹지는 40점 이하의 낮은 점수로 평가되었다. 환경조절적 기능이 낮은 지역의 경우 산림녹지에 대한 지속적인 복원 및 관리를 통해 기능성을 향상시켜야 할 것이다. 또한, 이상의 연구결과들은 지역계획, 도시계획, 환경계획 그리고 산림환경계획 등에서 보전지역 설정 및 개발지역 규제를 위한 기초자료로 활용될 수 있을 것으로 판단된다.

• Bulletin of the Korean Chemical Society
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• 제33권10호
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• pp.3248-3252
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• 2012

Vibrio extracellular metalloprotease (vEP), secreted from Vibrio vulnificus, shows various proteolytic function such as prothrombin activation and fibrinolytic activities. Premature form of vEP has an N-terminal (nPP) and a C-terminal (C-ter100) region. The nPP and C-ter100 regions are autocleaved for the matured metalloprotease activity. It has been proposed that two regions play a key role in regulating enzymatic activity of vEP. Especially, C-ter100 has a regulatory function on proteolytic activity of vEP. C-ter100 domain has been cloned into the E. coli expression vectors, pET32a and pGEX 4T-1 with TEV protease cleavage site and purified using gel-filtration chromatography followed by affinity chromatography. To understand how C-ter100 modulates proteolytic activity of vEP, structural studies were performed by heteronuclar multi-dimensional NMR spectroscopy. Backbone $^1H$, $^{15}N$ and $^{13}C$ resonances were assigned by data from standard triple resonance and HCCH-TOCSY experiments. The secondary structures of vEP C-ter100 were determined by TALOS+ and CSI software based on hydrogen/deuterium exchange. NMR data show that C-ter100 of vEP forms a ${\beta}$-barrel structure consisting of eight ${\beta}$-strands.

• 한국습지학회지
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• 제22권1호
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• pp.39-48
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• 2020

인공습지의 주요한 기능이자 조성된 인공습지의 목적 중 가장 많은 비율을 차지하는 기능은 수질정화 기능이다. 4대강사업 이후 국민들의 수질에 관한 관심과 요구가 증가하였고, 삶의 질 향상으로 인한 수변공간에 대한 활용도가 높아지면서 수질 개선에 대한 필요성은 더욱 증가할 것으로 보인다. 대부분의 인공습지 조성 사업 시 한 가지 목적에만 초점을 맞추는 게 대부분이며, 효과에 관한 연구도 한 가지 기능에 대한 효과를 분석하고 있어 실제 인공습지의 조성의 가치가 저평가 되고 있다. 따라서 인공습지를 조성에 따른 종합적 편익을 산정하기 위해 본 연구에서는 하천변 인공습지 조성에 따른 여러 효과 중 홍수저감 및 수질개선 기능에 대한 효과를 분석하고 이에 따른 편익을 산정하였다. 즉, 하천변에 가상의 인공습지를 설계 및 조성하여, 인공습지 조성 전·후의 비교를 통하여 홍수저감과 수질개선 효과를 산출하였고 이에 따른 편익을 각각 산정하였다.

• Journal of Ecology and Environment
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• 제33권1호
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• pp.15-18
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• 2010

Mangroves are special plant communities that live along intertidal zones in tropical and subtropical areas. They are regarded as one of the most important types of natural ecosystem in the world because of the many ecosystem functions that they perform, of which water purification is the most complex. Mangrove ecosystems are conducive to the deposition and retention of heavy metals. So it is important to understand the impact of heavy metals on mangrove ecosystems, and especially on soil subsystems. We examined the levels of heavy metals in the soil of mangroves in the Dongzhaigang Mangrove National Nature Reserve. Dongzhaigang, the first mangrove nature reserve established in China, is located south of Haikou in Hainan Island and encompasses $33.37\;km^2$, of which mangroves comprise $20.56\;km^2$. To assess the impact of human activities, we collected a large number of soil samples in four sampling areas (the protection station, the harbor, a tour area, and Yeboluo island) in the study area. We measured the concentrations of Cu, Pb, Zn and Cd in the soil samples using the spectra of polyatomic molecules. The average concentrations of Cu, Pb, Zn and Cd were $5.04\;{\mu}g/g$, $10.36\;{\mu}g/g$, $20.06\;{\mu}g/g$ and $0.06\;{\mu}g/g$, respectively, and the heavy metal concentrations were lowest in the protected area, highest in the harbor, and intermediate in Yeboluo Island and the tour area. The heavy metal concentrations in the soil collected from different sample plots are related not only to the physical and chemical properties of the soil, but also to the heavy metal emitted by nearby pollution sources. Our analysis indicates that tourist boats are the main pollution sources in the study area.