• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 제2회 추계심포지움
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• pp.143-145
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• 1994

Chimeric fusion proteins involving IgG have proven valuable in studying protein-protein interactions and may possess therapeutic applications as well. For example, three receptor subtypes for the natriuretic peptides, when fused to the Fc portion of human IgG ${\gamma}$ chain, were quantitatively and qualitatively indistinguishable from the native receptor, thus allowing detailed structure-function studies of the receptor. In an attempt to block human immunodeficiency virus infectivity with soluble derivatives of CD4, a CD4/IgG Fc chimeric molecule was shown to increase the plasma half life of soluble CD4 and possessed the added advantage of IgG Fc-mediated placental transfer. In the case of the KGFR, this approach provided a framework for dissection of its ligand binding domains and made it possible to demonstrate that high affinity binding sites for two ligands, aFGF and KGF, reside within different receptor Ig-like domains. Chimeric molecules fused to immunoglobulins would have the advantages of secretion from transfected cells as well as detection and purification from medium utilizing Staphylococcus aureus Protein A. In addition, where highly related receptors make their discrimination very hard due to the difficulties in generating specific immunochemical probes, IgG fusion protein with tailor-made specificities confers particular advantages to elucidate patterns of receptor distribution and expression. The approach described here may have general applications in defining ligand-receptor interactions as well as searching for specific agonists and antagonists of receptor function.

• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.468-473
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• 1996

The genome of tomato spotted wilt virus (TSWV) is composed of three RNA segments, S, M, and L RNA and the 5.0 kb M RNA encodes two glycoproteins Gl, G2 and NSm protein of unknown function. In an effort to investigate the function of the NSm protein, antibody was raised against NSm fusion protein overexpressed in Escherichia coli. This antibody was used to detect the NSm protein by using western blot analysis and electron microscopic observation after immunogold labelling. For the cloning of the NSm gene, total RNA extracted from a TSWV infected plant was used for cDNA synthesis and polymerase chain reaction (PCR) instead of going through time-consuming virus purification. A protein band specifically reacting to the NSm antibody was detected from TSWV inoculated plants. The NSm protein was detected in the cell wall fraction and in pellet from low speed centrifugation when the infected plant tissue was fractionated into 4 fractions. In the immuno-electron microscopic observation, gold particles were found around the plasmodesmata of infected plant tissue. These results suggest that the NSm protein of TSWV plays some role in cell-to-cell movement of this virus.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.631-638
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• 2008

Tandem affinity purification (TAP) method combined with LC-MS/MS is the most accurate and reliable way to study the interaction of proteins or proteomics in a genome-wide scale. For the first time, we used a TAP-tag as a mutagenic tool to disrupt protein interactions at the specific site. Although lots of commonly used mutational tools exist to study functions of a gene, such as deletional mutations and site-directed mutagenesis, each method has its own demerit. To test the usefulness of a TAP-tag as a mutagenic tool, we applied a TAP-tag to RNA polymerase II, which is the key enzyme of gene expression and is controlled by hundreds of transcription factors even to transcribe a gene. Our experiment is based on the hypothesis that there will be interrupted interactions between Pol II and transcription factors owing to the TAP-tag attached at the C-terminus of each subunit of Pol II, and the abnormality caused by interrupted protein interactions can be observed by measuring a cell-cycle of each yeast strain. From ten different TAP-tagged strains, Rpb7- and Rpb12-TAP-tagged strains show severe defects in growth rate and morphology. Without a heterodimer of Rpb4/Rpb7, only the ten subunits Pol II can conduct transcription normally, and there is no previously known function of Rpb7. The observed defect of the Rpb7-TAP-tagged strain shows that Rpb7 forms a complex with other proteins or compounds and the interruption of the interaction can interfere with the normal cell cycle and morphology of the cell and nucleus. This is a novel attempt to use a TAP-tag as a proteomic tool to study protein interactions.

• 한국농공학회지
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• 제44권5호
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• pp.96-105
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• 2002

As an useful water purification system for non-point source pollution in rural watersheds, interests in constructed wetlands are growing at home and abroad. It is well known that constructed wetlands are easily installed, no special managemental needs, and more flexible at fluctuating influent loads. They have a capacity for purification against nutrient materials such as phosphorus and nitrogen causing eutrophication of lentic water bodies. The Constructed Wetland Design Model (CWDM), developed through this study is consisted mainly of Database System, Runoff-discharge Prediction Submodel, Water Quality Prediction Submodel, and Area Assessment Submodel. The Database System includes data of watershed, discharge, water quality, pollution source, and design factors for the constructed wetland. It supplies data when predicting water quality and calculating the required areas of constructed wetlands. For the assessment of design flow, the GWLF (Generalized Watershed Loading Function) is used, and for water quality prediction in streams estimating influent pollutant load, Water Quality Prediction Submodel, that is a submodel of DSS-WQMRA model developed by previous works is amended. The calculation of the required areas of constructed wetlands is achieved using effluent target concentrations and area calculation equations that developed from the monitoring results in the United States. The CWDM is applied to Bokha watershed to appraise its application by assessing design flow and predicting water quality. Its application is performed through two calculations: one is to achieve each target effluent concentrations of BOD, SS, T-N and T-P, the other is to achieve overall target effluent concentrations. To prove the validity of the model, a comparison of unit removal rates between the calculated one from this study and the monitoring result from existing wetlands in Korea, Japan and United States was made. As a result, the CWDM could be very useful design tool for the constructed wetland in rural watersheds and for the non-point source pollution management.

• 식물조직배양학회지
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• 제28권6호
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• pp.347-351
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• 2001

인삼 (Panax ginseng C.A. Meyer)은 우리나라의 전통약재로 수 세기 동안 사용되어 왔으며, 약효 및 성분에 대한 연구가 매우 활발하게 이루어져 왔으나, 인삼의 분자생물학적, 단백질 화학적 측면에서 생리연구는 매우 미미하였다. 본 연구에서는 프로테옴믹스를 이용한 인삼 단백질 군을 연구하기 위한 첫 단계로 인삼 (천풍)및 모상근의 단백질 추출 방법을 확립하고, 이차원 전기영동을 통하여 추출방법의 유용성을 확인하였다. Homogenizer와 황산암모늄 침전을 통한 단백질 추출과 액체질소 및 TCA를 이용한 단백질 추출 방법을 수행하여 비교해 본 결과 추출 단백질 양에는 큰 차이가 없으나 이차원 전기영동 수행 시 액체질소 및 TCA를 통한 방법이 프로테옴 연구에 적합하여 훨씬 해상도가 높은 gel 이미지를 얻었을 뿐 아니라, gel당 660개 이상의 단백질 sopt을 확인하였다. 또한 인삼과 모상근의 이차원 전기영동을 비교한 결과 상당히 다른 발현 패턴을 보여, 생장조건 등 외부 환경과 생리상태의 차이에 따라, 같은 유전자를 가지고 있는 조직체라 할지라도, 매우 다른 프로테옴 (proteome)을 보일 수 있음을 보여주었다.

• 한국조사연구학회:학술대회논문집
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• 한국조사연구학회 2002년도 춘계학술대회 발표논문집
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• pp.8-34
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• 2002

이 연구의 주된 목적은 외국의 교육 및 심리검사를 번안하여 사용하는 경우, 번안된 검사와 原 검사간의 평형성 (equivalence)을 경험적으로 측정하기 위하여 차별문항기능기법을 응용하는 것이다. 즉, 평형성이라는 것은 타당한 그룹비교를 위한 하나의 전제조건으로서 이런 평형성이 확립되지 않은 채 번안된 검사들의 무분별한 사용은 바람직하지 않다. 서로 다른 접근법을 가지고 있는 두 개의 차별문항기능기법, 로지스틱 판별분석과 SIBTEST가 상호보완적인 목적으로 이용되었고, 최선의 대응변수를 모색하려는 시도로서 다변량 대응기법과 순환적인 정화기법이 사용되었다. 5개의 하위척도를 가진 외향성 척도 중 3개의 척도에서는 적은 수의 차별기능문항을 발견하였으나, 나머지 2개의 척도에서는 반 이상의 문항에서 차별기능을 추출하였다 마지막으로, 대응변수의 선택에 대한 문제들이 논의되고 있다.

• 한국해양바이오학회지
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• 제12권2호
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• pp.81-90
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• 2020

Sialic acid, which is contained in about 60-160 mg/kg in the edible bird's nest (EBN), is known to facilitate in the proper formation of synapses and improve memory function. The objective of this study is to extract effectively the sialic acid from edible bird's nest using affinity bead technology (ABT). After preparing the non-polar polymeric bead "KJM-278-28A" having a porous network structure, and then desorbed sialic acid was concentrated and dried. The analysis of the physicochemical properties of bead "KJM-278-28A" showed that the particle size was 400-700 ㎛, the moisture holding capacity was 67-70%, the surface area (BET) was 705-900 ㎡/g, and the average pore diameter 70-87 Å. The adsorption capacity of the bead "KJM-278-28A" for sialic acid was shown a strong physical force to bind sialic acid to the bead surface of 400 mg/L. In addition, as a result of analyzing the adsorption and desorption effects of sialic acid on water, ethanol, and 10% ethanol on the bead, it was confirmed that desorption effectively occurs from the beads when only ethanol is used. As a result of HPLC measurement of the separated sialic acid solution, a total of four sialic acid peaks of N-acetyl-neuraminic acid (Neu5Ac), α,β-anomer of Neu5Ac and N-glycoly-neuraminic acid were identified. Through these results, it was confirmed that it is possible to separate sialic acid from EBN extract with efficient and high yield when using ABT.

• Journal of Microbiology and Biotechnology
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• 제6권3호
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• pp.162-166
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• 1996

The protein coding sequence of the 4-aminobutyrate aminotransferase was amplified by polymerase chain reaction (PCR) from a previously cloned cDNA of pig brain using a pair of primers based on the published sequence. The amplified DNA was introduced into a T7 expression vector. Recombinant 4-aminobutyrate aminotransferases were overexpressed in Escherichia coli. The inclusion bodies were formed when enzyme was overexpressed. The unfolded, overproduced proteins were purified by chromatography with hydroxyapatite and refolded by a sequential dialysis method. The renatured 4-aminobutyrate aminotransferase regained the catalytic activity. However, the purified mutant protein did not show the catalytic function of 4-aminobutyrate aminotransferase.

• 상하수도학회지
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• 제23권6호
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• pp.727-733
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• 2009

This study was performed to investigate the physical characteristics like compressive strength, permeability, porosity and the water quality removal characteristics of permeable concrete pavement filled with microbial-soil sheet to remove SS, organic matter and nutrients in artificial rainfall. As a result, it can show the removal efficiency is SS 90~95%, COD 85~93%, BOD 80~83%, T-N 61~75%, T-P 71~78% on WAPS I(W1) and WAPS II(W2). Therefore, permeable concrete pavement filled with microbial-soil sheet shows higher removal efficiency(SS 10%, organic matter and nutrients 30%) than a conventional porous concrete(W3). By filling microbial-soil sheet to permeable concrete pavement, we confirm that the function and efficiency are improved significantly and that a naturally-friendly facility can be developed and applied to treat non-point sources.

• Journal of Advanced Marine Engineering and Technology
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• 제25권3호
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• pp.623-630
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• 2001

The conventional control techniques based a mathematical model are not well suited for dealing with ill-defined and uncertain system like a linear quadratic control. Recently, fuzzy control has been successfully applied to a wide variety of practical problems such as robot, water purification, automatic train operation system etc. In this paper, a design technique of robust Fuzzy-LQ controller for each subsystem is designed. Secondly , all the subsystem controllers are combined by fuzzy weighted averaging method. Finally the effectiveness of the proposed controller is verified through a series of computer simulations for an inverted pole system.