• 농촌계획
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• 제21권4호
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• pp.45-53
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• 2015

Agriculture, rural landscapes are accompanied by a variety of environmental issues. Therefore, it is necessary to study on biodiversity and ecosystem services. Horticulture complex is low groundwater recharge function, it can be evaluated as a facility that biodiversity is impaired. The ecosystem services in agricultural landscapes were obtained 19 kinds of functions. Experts survey Groundwater recharge function (4.13) teeth chapter higher, Water storage (4.05), Amphibian & Reptile habitat (3.96), Aquatic insect habitat (3.92), Flood control (3.87), Water purification (3.86), Avian habitat (3.76 ), Creating landscape (3.74), Vegetation diversity (3.71), Experience, Education (3.69), Biological control (3.48), Fishery habitat (3.42), Climate regulation (3.30), Mammal habitat (3.30), Air quality regulation (3.25 ), Mainenance of genetic diversity (3.25), were analyzed in order Rest area (3.14). Improving capabilities in the Detention Pond, Wetland, Green space, Corridor, Non-Chemical, Program development, Green spaces, Rainwater storage facilities, Water cycle system, Surface water storage facilities, Infiltration trench, Water purification facilities, Permeable pavement. Environmentally friendly, and to contribute to sustainable agricultural development through ecological planning.

• 환경영향평가
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• 제20권6호
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• pp.815-821
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• 2011

This study aims to estimate the biological decomposition capacity of MPC(Microorganism Porous-Concrete). MPC has specific surface area formed by inside pores, and bio compound was added to those pores to reduce pollutants loading. To evaluate the water purification capacity of MPC, we carried out the comparative studies using different media types [GPC(General Porous-concrete), CPC(Compound porous-concrete), LPC(Lightweight aggregate porous-concrete)] under the condition of CFSTR, and different retention times (30, 60 and 120 min). We also estimated the purification capacity of MPC under different concentrations of pollutant loadings. The MPC showed higher efficiency in water purification function than other conventional porous concretes with efficient decrease rates of SS, BOD, COD, and nutrient concentrations. In the comparison experiment for different retention times, MPC showed the highest removal efficiency for all tested pollutants in the longest retention time(120 min). In the long period test, the removal efficiencies of MPC concrete were high until 100 days after the set up of the operation, but began to decrease. Outflow flux was invariable compared with inflow flux so that extra detention time for media fouling such as back washing is not needed. But the results suggested that appropriate management is necessary for long-term operation of MPC. As the final outcome, MPC using bio organisms is considered to be efficient for stream water purification when they used as substrates for artificial river structure.

• BMB Reports
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• 제53권3호
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• pp.154-159
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• 2020

We investigated the effects of physalin A, B, D, and F on osteoclastogenesis induced by receptor activator of nuclear factor κB ligand (RANKL). The biological functions of different physalins were first predicted using an in silico bioinformatic tool (BATMAN-TCM). Afterwards, we tested cell viability and cell apoptosis rate to analyze the cytotoxicity of different physalins. We analyzed the inhibitory effects of physalins on RANKL-induced osteoclastogenesis from mouse bone-marrow macrophages (BMMs) using a tartrate-resistant acid phosphatase (TRAP) stain. We found that physalin D has the best selectivity index (SI) among all analyzed physalins. We then confirmed the inhibitory effects of physalin D on osteoclast maturation and function by immunostaining of F-actin and a pit-formation assay. On the molecular level, physalin D attenuated RANKL-evoked intracellular calcium ([Ca(2+)](i)) oscillation by inhibiting phosphorylation of phospholipase Cγ2 (PLCγ2) and thus blocked the downstream activation of Ca2+/calmodulin-dependent protein kinases (CaMK)IV and cAMP-responsive element-binding protein (CREB). An animal study showed that physalin D treatment rescues bone microarchitecture, prevents bone loss, and restores bone strength in a model of rapid bone loss induced by soluble RANKL. Taken together, these results suggest that physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via suppressing the PLCγ2-CaMK-CREB pathway.

• 한국자기공명학회논문지
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• 제15권1호
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• pp.25-39
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• 2011

Syndecan-4 is a transmembrane heparan sulfate proteoglycan, which is a coreceptor with integrins in cell adhesion. To get better understand the mechanism and function of Syndecan-4, it is critical to elucidate the three-dimensional structure of a single transmembrane spanning region of them. Unfortunately, it is hard to prepare the peptide because syndecan-4 is membrane-bound protein that transverse the lipid bilayer of the cell membrane. Generally, the preparation of transmembrane peptide sample is seriously difficult and time-consuming. In fact, high yield production of transmembrane peptides has been limited by experimental adversities of insufficient yields and low solubility of peptide. Here, we demonstrate experimental processes and results to optimize expression, purification, and NMR measurement condition of Syndecan-4 transmembrane peptide.

• 한국환경복원기술학회지
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• 제9권3호
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• pp.1-16
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• 2006

The purpose of this study is to develop design model of ecological park as stormwater storage facilities. The results are as follows : First, the design model of ecological park as stormwater storage facilities consider ecological and landscape characteristics such as high efficiency of land use, function as disaster prevention, ecological water purification, formation of habitat for flora and fauna. Second, this study demonstrates two types of plane structure and eight types of designed section. They can be combined and designed depending on conditions of each site. The facilities of stormwater storage conduct disaster prevention system and ecological park. Retention pond in stormwater storage facilities for ecological park also should be made for ecological restoration in the site. Third, the ecological park provide the basis for ecological network from in-site to out-site. Therefore its conservation and restoration plan consider the ecosystems of the site. Fourth, the most important factor for maintenance and management for retention pond is keeping water quality. Sustainable Structured wetland Biotop system is suggested for ecological water purification system in the retention pond which is one of the constructed wetland system using multi-celled aquatic plant and pond. This system can also provide habitat for animals and plants, water friendly park for men, and beautiful landscape.

• 한국해양공학회지
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• 제21권3호
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• pp.8-15
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• 2007

This study is intended to examine the relationship between the magnitude of sand spit in the estuary of the stream and improvement of the quality of water that flaws into the sea, by means of hydraulic experimentation. In order to determine the effect of improvements of water quality when river flow is stagnant, the estuary flows into the sea through the small sand spit, and a two-dimensional physical model experiment was carried out. Distribution of concentration was decreased in response to an increase in length of sand spit and time. The experimental results are compared with theoretical results, based on the solution of the equation. Also, there are functions of influx prevention of salt wedge and purification of pollution water due to sand spit in small estuary.

• 식물조직배양학회지
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• 제27권4호
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• pp.283-297
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• 2000

Seeds are an ideal repository for recombinant proteins in molecular farming applications. However, in order to use plant seeds efficiently for the production of such proteins, it is necessary to understand a number of fundamental biological properties of seeds. This includes a full understanding of promoters which function in a seed-specific manner, the subcellular targeting of the desired polypeptide and the final form in which a protein is stored. Once a biologically active protein has been deposited in a seed, it is also critical that the protein can be extracted and purified efficiently. In this review, these issues are examined critically to provide a number of approaches which may be adopted for production of recombinant proteins in plants. Particular attention is paid to the relationship between subcellular localization and protein extraction and purification. The robustness and flexibility of seed-based production is illustrated by examples close to or already in commercial production.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 추계학술대회 논문집
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• pp.383-386
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• 2008

The four-bed PSA process using a layered bed of activated carbon and zeolite 5A was studied to produce a high purity hydrogen product from SMR off-gas. At a desired product purity (99.999%+), the recovery increased with decreasing the linear velocity. However, the difference of the increasing of the recovery became smaller with the decreasing of the linear velocity and then was similar from below the linear velocity 3.9 cm/s. When the adsorbents, the feed gas composition, and the operating conditions are given, the residence time is mainly a function for design of the PSA bed size. The minimum residence time exists to obtain the maximum recovery at desired product purity.

• Archives of Pharmacal Research
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• 제20권6호
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• pp.590-596
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• 1997

The NADH reductase component of the steroid 9.alpha.-hydroxylase from Mycobacterium fortuitum was purified to homogeneity. Recovery of the enzyme from the 50-60% ammonium sulfate saturated fraction was 49%, with a purification factor of 100-fold. The NADH reductase has a relative molecular of 60 KDa as determined by SDS-PAGE. The absorption maxima at 410 and 450 nm indicate the presence of iron-sulfur group and flavin. These prosthetic groups seemed to function as redox groups that transfer electrons from NADH to the following protein. The $K_M$ value for NADH as substrate was $68{\mu}M$. The $NH_2$-terminal amino acid sequence of the reductase was determined as Met-Asp-Ala-Ile-Thr-Asn-Val-Pro-Leu-Pro-Ala-Asn-Glu-Pro-Val-His-Asp-Tyr-Ala-Thr. This sequence does not show a homology with the $NH_2$ -terminal sequences reported for the reductase component of other monooxygenases, suggesting that the NADH reductase component of the steroid 9.alpha.-hydroxylase system is novel.

• BMB Reports
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• 제42권11호
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• pp.697-704
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• 2009

Membrane proteins play important roles in the biology of the cell, including intercellular communication and molecular transport. Their well-established importance notwithstanding, the high-resolution structures of membrane proteins remain elusive due to difficulties in protein expression, purification and crystallization. Thus, accurate prediction of membrane protein topology can increase the understanding of membrane protein function. Here, we provide a brief review of the diverse computational methods for predicting membrane protein structure and function, including recent progress and essential bioinformatics tools. Our hope is that this review will be instructive to users studying membrane protein biology in their choice of appropriate bioinformatics methods.