• Reproductive and Developmental Biology
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• 제31권2호
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• pp.103-108
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• 2007

This study was carried out to investigate the effects of cryoprotectants, warming solution and removal of lipid on open pulled straw vitrification (OPS) method of porcine embryos produced by nuclear transfer (NT) of fetal fibroblasts. All solutions used during vitrification were prepared with holding medium consisting of 25 mM Hepes buffered TCM199 medium containing 20% fetal bovine serum (FBS) at $38.5^{\circ}C$. The blastocysts derived from NT with or without lipid were vitrified in each medium of different concentrations of dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Also, blastocysts after cryopreservation were warmed into different concentrations of sucrose in warming solution. The optimal concentrations of cryoprotectants in vitrification solution were 10% DMSO + 10% EG in vitrification solution 1 (VS1) and 20% DMSO + 20% EG in vitrification solution 2 (VS2). The optimal concentrations of sucrose were 0.3 M sucrose in warming solution 1 (WS1) and 0.15 M sucrose in warming solution 2 (WS2). lipid removal from oocytes before NT enhanced the viability of NT embryos after vitrification. Our results show that use of the OPS method in conjunction with lipid removal provides effective cryopreservation of porcine nuclear transfer embryos.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.84-84
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• 2002

The advantages of the OPS techniques(Vajta G et al, Mol Reprod Dev 51: 53-58,1998) give 1) high survival rates of various types of eggs, 2) quick and simple process, 3) inexpensive equipment and reduced chilling injury. The efficiency of IVM/IVF technique in the porcine species is relatively lower than that obtained in other species such as ruminants. Two experiments were designed to investigate the effects of in-vitro fertilization of porcine oocytes matures using different OPS protocol for chilling and warming of vitrification. Porcine oocytes from ovaries collected at abattoir were matured for 44 hours in TCM199 Earle's salt supplemental with pyruvate, pff, L-cysteine, hormones and gentamycin. Oocytes were denuded and fertilized with frozen boar semen by common method. Porcine embryos produced routinely by in-vitro culture system of NCSU23 medium. The vitrification and the warming were conducted by OPS method with the glass micropipette instead of straw vessels and modified the protocol of G.Vajta(1999). In Exp 1, Chilling/Warming:Holding Medium(HM)+EG+DMSO/HM +sucrose Medium(SM) at 39$^{\circ}C$ warm stage. In Exp 2, : PBS+CS+EG+Ficoll+ Trehalose/PBS+Trehalose at 25$^{\circ}C$ stage. Filling, freezing, packing, thawing out and further culturing were performed to follow the basic protocol of G Vajta. During IVM-lVC and post-warming, fertilization parameter and developmental potential were compared to and statistically analysed. It was not significantly different from Exp 1 and Exp 2 but 25$^{\circ}C$ of stage was slightly higher on the morula/blastocyst forming rate and better atmosphere for worker than that at 39$^{\circ}C$ stage.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 추계학술대회 논문집
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• pp.362-365
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• 2005

Currently Chemical Mechanical Planarization (CMP) has become an essential step in the overall semiconductor wafer fabrication technology. Especially the CMP pad conditioner, one of the diamond tools, is required to have strong diamond retention. Strong cohesion between diamond grits and metal matrix prevents macro scratch on the wafer. If diamond retention is weak, the diamond will be pulled out of metal matrix. The pulled diamond grits are causative of macro scratch on wafer during CMP process. Firstly, some results will be reported of cohesion between diamond grits and metal matrix on the diamond tools prepared by three different manufacturing methods. A measuring instrument with sharp cemented carbide connected with a push-pull gauge was manufactured to measure the cohesion between diamond grits and metal matrix. The retention force of brazed diamond tool was stronger than the others. The retention force was also increased in proportion to the contact area of diamond grits and metal matrix. The brazed diamond tool has a strong chemical combination of the interlayer composed of chrome in metal matrix and carbon which enhance the interfacial cohesion strength between diamond grits and metal matrix. Secondly, we measured real-time data of the coefficient of friction and the pad wear rate by using CMP tester (CETR, CP-4). CMP pad conditioner samples were manufactured by brazed, electro-plated and sintered methods. The coefficient of friction and the pad wear rate were shown differently according to the arranged diamond patterns. Consequently, the coefficient of friction is increased according as the space between diamonds is increased or the concentration of diamonds is decreased. The pad wear rate is increased according as the degree of diamond protrusion is increased.

• 한국정보시스템학회지:정보시스템연구
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• 제30권3호
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• pp.113-135
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• 2021

Purpose This study aims to measure the effects of media literacy education through the social science method and find out the impact of media literacy education on digital citizenship in Korea and its implications for the development of democracy. Design/methodology/approach This study used an independent t-test to analyze relativeness between media literacy education and media literacy factors. We also adopted an independent t-test to investigate media literacy influence on digital citizenship. Furthermore, we found out age differences using 'the one-way analysis of variance (the one-way ANOVA)'. Findings We have found four media literacy factors, MC, CT, C&P, R&R, have been significantly affected by media literacy education. We have also pulled out three digital citizenship factors, IPA, TS, and CP, involved in media literacy education. Moreover, we have analyzed our survey by different age groups. The lower the age, the higher the TS. CP was highest among those from 30 to 59, followed by those under the 30s.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1995년도 춘계학술대회
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• pp.19-22
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• 1995

A biomechanical study was made to demonstrate the superior mechanical performance of the newly designed Miniplate staple to the conventional Coventry staple in high tibial osteotomy(HTO). Using twenty fresh porcine tibiae, the fixational strengh of the two different types of staple in HTO was compared. To minimize the error due to the specimen-to-specimen individuality, the bone mineral density of the tibiae was measured with a bone densitometry (Dual photon absorptionometer, Luner, USA) and those with $0.8\;{\sim}\;1.2\;gm/cm^2$ at the proximal tibia was used in the biomechanical test. Testing was performed on a material testing system (Autogram ET-5, Shimatzu, Japan) with aid of a commercial data processor (IBM 80386/ ASYST). Using two differant loading modes, 'pull-out' and 'push-out', the maximum resistant force required to release the staple from the substrate bone was recorded. In the pull-out test, ten non-osteotomized specimens were used and the staple was pullout by subjecting an axial tension on the head of the staple inserted. While in the pull-out test where ten tibiae osteotomized in the usual way of HTO were used, the staple was not directly loaded. In this testing, as a mimic condition of the natural knee, the distal part of the specimen tibia was pushed horizontally in order for the staple to be pulled out while the proximal tibia was fixed. The pull-out strength of Coventry staple and miniplate staple were found to be $27.88\;{\pm}\;5.12\;kgf$ and $182.47\;{\pm}\;32.75\;kgf$, respectively. The push-out strength of Coventry staple and miniplate staple were $18.40\;{\pm}\;4.47\;kgf$ and $119.95\;{\pm}\;19.06\;kgf$, respectively. The result revealed that miniplate staple had the pull-out/ push-out strength at least fivetimes higher than Coventry staple. Based on the measured data, it was believed that the newly designed miniplate staple could provide much better postoperative fixation in HTO. The postoerative application of long leg casting may not be needed after HTO surgery.

• Journal of Chest Surgery
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• 제20권3호
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• pp.570-573
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• 1987

A surgical operation on a patient with infected left atrial myxoma is presented. The patient was a 50-year-old woman who was admitted to the Kyung Hee Medical Center Hospital on March 7 1987 with complaints of high fever, cough and dyspnea that occurred a few days prior to admission. She, who no definite history of the previous cardiac-related problems was obtained from, had visited a dentist to have her eight teeth pulled out a few months prior to this admission. A preoperative 2-D echocardiogram revealed a left atrial tumor, strongly suggesting myxoma and two blood cultures drawn prior to surgery yielded streptococcus viridans. Under the preoperative impression of an infected left atrial myxoma, the tumor was removed through a biatrial approach after establishing CPB and cold cardioplegic. Pathologic examination of the tumor demonstrated a typical myxoma with bacterial colonies within. Postoperative course was uneventful and she was completely well at discharge from the hospital. To our best knowledge, this report is the first surgical experience of infected left atrial myxoma in the Korean literature.

• 한국분말재료학회지
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• 제9권3호
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• pp.148-152
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• 2002

The microstructures and indentation fracture of pressureless-sintered $DyNbO_4$ crystalline were investigated as a basic study for the application of weak phase of fibrous monolithic composites. They were comprised with many lamella twins as well as micro-cracks at the grain boundaries. The hardness at room temperature was remarkably low value(575 Hv) due to the low relative density and existence of microcracks at grain boundaries. The main fracture mode was a typical intergranular fracture, and showed remarkable micro-cracking effect. The heavy plastic deformation was observed around the site of indentation. In addition, the $DyNbO_4$ was expected to apply as a weak phase in the fibrous monolithic composites because of the low hardness and easily plastic deformation that could be led the preferable pulled-out and microcracking toughening under the failure.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 봄 학술발표회 논문집
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• pp.1091-1096
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• 2003

Pullout tests of single headed bars using plain concrete blocks indicate that the embedment depth of $10d_b$ is in general required for the headed bars to develop pullout strength equivalent to 125% of bar yield strength. In this experimental study, test results of multiple headed bars installed in reinforced concrete column sections are presented. Test variables included embedment depth, column main reinforcement ratio, and spacing of column ties. 2D29 bars were pulled out at one time from normal strength concrete. Test results indicated that the embedment depths, column tie spacings, and column main reinforcement ratios all influenced the pullout strengths of the headed bars. When the embedment depth was not sufficient, narrow tie spacings especially resulted in increased pullout strengths of the headed bars. Test results also indicated that the embedment depth of 15㏈ was sufficient for the closely spaced two headed bars (head-to-head spacing =$6d_b$) to develop pullout strength equivalent to 125% of the bar yield strength.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 봄 학술발표회 논문집
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• pp.567-572
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• 2003

An experimental work is presented to evaluate the retrofit method for improving the flexural capacity of shear walls. Fives shear wall specimens are designed and retrofitted by using carbon fiber materials such as rod, sheet and plate. Cyclic horizontal loads are applied to the specimens under constant axial load, $0.1f_{ck}A_g$. Test result shows that specimens with additional flexural reinforcement have the increased initial stiffness and deformation capacity. However, the strength is not improved as much as expected. This is because that the flexural reinforcement is pulled out from the foundation at the latter half of cycles. In order to maximize the flexural retrofit, therefore, it is required to study the anchorage behavior of the flexural reinforcement for retrofit.

• 대한전자공학회논문지
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• 제27권1호
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• pp.10-19
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• 1990

A fault tolerant control system, in which a digital back-up controller system is added on the existing analog control system, is developed for enhancing reliability of boiler control system in power plant. The digital back-up controller system(DBCS) has a multi-processor structure with capabilities of fault diagnosis, back-up control, self test, and graphic monitoring. Specifically, switching mechanism composed of expandable modules is designed so that back-up controller takes over any faulty control loops and the number of back-up control loops is determined as that of simultaneous faults. A process simulator that simulates the boiler analog control system is developed for safety test and performance evaluation prior to real plant application. DBCS is installed at the Ulsan thermal power plant, and fault tolerant control performance is assured under the faults that some controller modules are pulled out.