• Bulletin of the Korean Chemical Society
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• v.31 no.11
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• pp.3241-3246
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• 2010

NHERF ($Na^+/H^+$ exchanger regulatory factor) and E3KARP (NHE3 kinase A regulatory protein) play important roles in membrane targeting, trafficking and sorting of ion channels, transmembrane receptors and signaling proteins in many tissues. Each of these proteins contains two PDZ (PSD-95/Dlg-1/ZO-1) domains, which mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes. The interaction between NHERF and E3KARP was investigated by surface plasmon resonance spectroscopy (BIAcore), fluorescence measurement, His-tagged pull-down experiment, and size-exclusion column (SEC) chromatography. BIAcore experiments revealed that NHERF bound to E3KARP with an apparent $K_D$ of 7 nM. Fluorescence emission spectra of the NHERF-E3KARP complex suggested that the tight interaction between these proteins was accompanied by significant conformational changes in one or both. The CD spectra of NHERF and E3KARP show that the conformational changes of these proteins were dependent on pH and temperature. These results implicate that the NHERF-E3KARP complex allows intracellular signaling complexes to form through PDZ-PDZ interactions.

• Bulletin of the Korean Chemical Society
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• v.26 no.1
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• pp.77-84
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• 2005

We prepared the new nonlinear optical chromophores that show fairly high microscopic nonlinearity through intramolecular charge transfer. Phenothiazine and carbazole units played an important role to contribute high electron donability and connect the resonance pathway via conjugative effect in the cyclized ring beside the aromatic ring. Theoretical calculation, electrochemical analysis, and absorption spectroscopic study gave us useful information about the energy states and microscopic nonlinearities of two serial chromophores. We compared the microscopic nonlinearities of four chromophores with the conjugation length and electron donability in the push-pull type NLO chromophores. The effect of gradient donability and lengthening the conjugation were investigated on the electronic state and microscopic nonlinearity.

• Journal of KIISE:Databases
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• v.31 no.3
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• pp.297-306
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• 2004

Push-based broadcasting in wireless information services is a very effective technique to disseminate information to a massive number of clients when the number of data items is small. When the database is large, however, it nay be beneficial to integrate a pull-based (client-to-server) backchannel with the push-based broadcast approach, resulting in a hybrid data delivery. In this paper, we analyze the performance behavior of a predeclaration-based transaction processing, which was originally devised for a push-based data broadcast, in the hybrid data delivery through an extensive simulation. Our results show that the use of predeclaration-based transaction processing can provide significant performance improvement not only in a pure push data delivery, but also in a hybrid data delivery.

• Korean Journal of Materials Research
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• v.29 no.6
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• pp.378-384
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• 2019

Ice accumulation on Aluminum Conductor Steel Reinforced(ACSR) cable during winter is an important matter in terms of safety, economy, and efficient power supply. In this work, the ice adhesion strengths of ACSR cable oxidized during different periods(7 years oxidized and 15 years oxidized) are evaluated. At first, a plate type dry oxidation standard specimen, whose surface characteristics are similar to those of ACSR cable, is prepared. Dry oxidation standard specimens are heat-treated at $500^{\circ}C$ for 20, 60, and 120 minutes in order to obtain different degrees of oxidation. After the dry oxidation, surface properties are analyzed using contact angle analyzer, atomic force microscopy, spectrophotometer, and gloss meter. The ice adhesion strengths are measured using an ice pull-off tester. Correlations between the surface properties and the ice adhesion strength are obtained through a regression analysis indicating a Boltzmann equation. It is revealed that the ice adhesion strength of 15-year oxidized ACSR cable is approximately 8 times higher than that of ACSR-bare.

• Journal of the Korean Society of Industry Convergence
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• v.25 no.5
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• pp.861-868
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• 2022

The purpose of this study is to design an effective atmospheric environment system through the design of the dust collection in the air shot room being operated in a domestic shipyard. The ventilation system in the current air shot room mostly uses a dust collecting filter to filter internal particles and releases them in the atmosphere. A conventional design was made too much. In order to prevent an error and draw an optimal design, Computational fluid dynamics (CFD) tried to be applied only to air shot room. In the advanced design technique, computer simulation was conducted to secure basic design data. In order to find the basic design of the ventilation system and the flow field in the air shot room at propeller mold workplace of a shipyard, the CFD was conducted. In the case of Model-1 as a conventional workplace, where air flows in the inlet due to the subatmospheric pressure generated by inhalation of an air blower and flows out to the outlet, a discharge flow rate was somewhat low, and there was the holdup zone in the room. In the case of Model-2 as an improved model, the ventilation system was improved in the Push-Pull type, and the holdup of the internal flow field was improved.

• Advances in concrete construction
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• v.14 no.2
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• pp.139-151
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• 2022

Filling materials poured into precast member joint are subjected to restraint stress by the precast member and joint reinforcement. The induced stress will likely cause cracks at early ages and performance degradation of the entire structure. To prevent these issues and design reasonable joints, it is very important to analyze and evaluate the restrained shrinkage cracks of filling materials at various restraint conditions. In this study, a new time zero-that defines the shrinkage development time of a filling material-is proposed to calculate the accurate amount of shrinkage. The tensile stresses and strengths at different ages were compared through the ring test (AASHTO PP34) to evaluate the crack potential of the restrained filling materials at various restraint conditions. The mixture which contained an expansive additive and a shrinkage reducing agent exhibited high resistance to shrinkage cracking owing to the high-drying shrinkage compensation effect. The high-performance, fiber-reinforced cement composite, and ultra-high-performance, fiber-reinforced cement composite yielded very high resistance to shrinkage and cracking owing to the pull-out property of steel fibers. To this end, multiple nonlinear regression analyses were conducted based on the test results. Accordingly, a modified tensile stress equation that considered both the geometric shape of the specimen and the intrinsic properties of the material is proposed.

• Journal of the Korean Society for Precision Engineering
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• v.13 no.7
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• pp.45-58
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• 1996

We provide a set of information on technologies in the area of mechanical engineering to draw meaningful action plans for the internationalization of National R&D activities. In this study, we employed a modified Delphi method to evaluate levels of our technoligical capabilities and developed countries' as well. We investigate technology acquistion methodologies, technology charcteristics and various aspects of interna- tional cooperation in terms of technology. Then, we analyzed final responses of participants(i.e., the third round results of Delphi method) to see the correlation among various factors in developing mechanical engineeing technologies through international cooperation. The technology classification used in this research was devel- oped by STEPI(Science and Technology Policy Institute). In conclusion, our mechanical engineering technology is investigated to be below the middle level stage of technology(i.e., the stage of digesting acquired technologies) except the shipbuilding equipments technology which is evaluated to be on the top level stage of technology.

• Korean Journal of Agricultural Science
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• v.49 no.1
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• pp.151-162
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• 2022

This study was conducted to develop a rail-type boom sprayer, performing safe spraying with improved ergonomic postures during pesticide spraying of agro-photovoltaic power system. The sprayer was designed to reduce labor cost and to be safe from pesticide exposure, and was analyzed through a comparison with a conventional spray method. The rail-type boom sprayer, consisted of a self-propelled spray and hose winder, hose, and boom sprayer parts, was designed to automatically pull and spray in the vertical and horizontal directions. The performance of the sprayer for an agro-photovoltaic power system was appropriate. From the analysis of postures with the Ovako Working posture Analysis System (OWAS), Rapid Entire Body Assessment (REBA), and Rapid Upper Limb Assessment (RULA) methods, the musculoskeletal risk factors to the body using the rail-type boom sprayer were less than those with the conventional power sprayer. In addition, the possibility of pesticide poisoning was reduced compared to the conventional power sprayer. The working capacity with the rail-type boom sprayer was more than five times greater, compared with the conventional power sprayer. After performing pesticide spraying with the rail-type boom sprayer, the labor cost was reduced to 42,750 won·yr^-1, which was 90% (402,750 won·yr^-1) less than the cost with the use of a conventional power sprayer (445,500 won·yr^-1).

• Journal of the Korean Society for Precision Engineering
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• v.14 no.6
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• pp.74-82
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• 1997

We provide a set of information on technologies to draw meaningful action plans for the internationalization on National R&D activities. In this study, we employed a modiffed Delphi method to evaluate levels of our technological capabilities and developed countries as well. We investigated technology acquis- tion methodologies, technology characteristics and various aspects of international cooperation in terms of technology. Then, we analyzed final responses of participants (i.e., the third round results of Delphe method) to see the correlation among various factors in developing technologies through international R&D cooperation. The technology classification used in this research was developed by STEPI (Science and Technology Policy Institute). In conclusion, levels of our technologies were investigated to be between the stage of imi- tating/absorbing advanced thechnologies and the stage of digesting acquired technologies. Especially, com- paring with other technologies, mechanical engineering was evaluated to be almost near the stage of digesting acquired technology(the middle level stage of technology). Our overal technological capabilities were evaluated to be 40 .approx. 60% of top level countries.

• Korean Journal of Microbiology
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• v.50 no.2
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• pp.95-100
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• 2014

Hrq1 is a novel member of RecQ helicase family, found in fungal genomes by bioinformatics analyses. It is most homologous to human RECQL4 and recent genetic and biochemical studies suggested that it may play roles in the maintenance of genome stability. In this study, we investigated yeast two-hybrid interactions between Hrq1 and the yeast genes homologous to the human genes that are known to interact with RECQL4. Among the 11 genes tested, Rad14, a nucleotide excision repair (NER) factor, was found to interact with Hrq1. In addition, pull-down assay with the purified proteins revealed direct protein-protein interaction between Hrq1 and Rad14. The yeast two-hybrid interaction was enhanced by the DNA damage induced by 4-nitroquinoline-1-oxide, which was dependent on the presence of Rad4, a key NER factor. These results suggest that Hrq1 may function in NER through interaction with Rad14.