• Title/Summary/Keyword: Pseudogobio esocinus

Search Result 39, Processing Time 0.019 seconds

Purification, Kinetics and Immunochemistry of Two Homotetrameric Lactate Dehydrogenase Isozymes in Pseudogobio esocinlus (Cypriniformes) (Pseudogobio esocinus (Cypriniformes) 젖산수소이탈효소 동질사량체들의 정제, 역학 및 면역화학)

  • 김명옥;염정주
    • The Korean Journal of Zoology
    • /
    • v.32 no.4
    • /
    • pp.420-428
    • /
    • 1989
  • Pseudogobio esocinus의 심장, 신장 및 간 조직은 하부단위체 C를 함유하는 젖산수소이탈효소를 갖고 있음이 확인되었다. 하부단위체 A 및 B에 대한 유전자들의 조직 발현은 다른 포유동물의 것과 유사하였으며 분자량은 140,000 정도로 추정되었다. Oxamate gel을 사용한 chromatography결과 A4 동위효소는 NAD+보다는 column buffer에 의해 용출되었다. B4 동위효소는 CM-Sepharose column을 사용하여 부붙 정제되었다. B4 동위효소는 물론 A4 동위효소도 고농도의 Pyruvate에 의해 저해되었다. A4 동위효소의 affinity chromatography 상 행동과 Pyruvate 저해 정도로 보아 A4 등위효소는 B4 동위효소 두 역학적으로 유사하다고 사료된다. P. esainus A4 동위효소에 대한 항체는 mouse A4 등위효소와 반응하지만 동종의 B4 동위 효소와는 반응하지 않는 특성으로 보아 하부단위체 B는 진화과정에서 보존성이 낮은 것으로 사료된다. Three tissues of heart, kidney and liver of a primitive cvprinid Pseudogobio esocinus were found to have lactate dehydrogenase isozyme(5) containing subunit C. Tissue expressions of genes for subunits A and B were similar to those of mammalian species. Molecular weight of the isozymes were estimated to be 140,000 approximately. Affinity chromatography of the isozymes on the immobilized oxamate gel revealed that A4 isozyme was not elected in NAD+ but in column buffer. B4 isozune was isozpnatically purified by subjecting kidney extract to a CM-Sepharose column. Ae isozvme as well as B4 isozvme was inhibited by high concentrations of pyruvate. The affinity chromatographic behavior and susceptibility to pyruvate inhibition of the A4 isorpne suggest that A4 isozwne is similar to B4 isozyme kinetically. Antibodies against p. esocinus A4 isogyme reacted with mouse At isozyme but not with p. esocinus B4 isogyme, reflecting that subunit B is less conservative in its evolution.

  • PDF

Ostelogical Development of Larvae and Juveniles of Pike Gudgeon Pseudogobio esocinus (Cypriniformes: Gobionidae) from Boseonggang River, Jeollanam-do, Korea (전라남도 보성강에 서식하는 모래무지(Pseudogobio esocinus) (잉어목: 모래무지과) 자치어의 골격 발달)

  • Ae-Ri Jung;ChongMin Han;Jin Lee;Kyeong-Ho Han;Sung-Hoon Lee
    • Korean Journal of Ichthyology
    • /
    • v.36 no.2
    • /
    • pp.129-138
    • /
    • 2024
  • This study examines the osteological development of the Pike gudgeon, Pseudogobio esocinus in the cranial, vertebral, caudal, pelvic and pectoral girdle for the purpose of taxonomic and aquacultural studies. Adult Pike gudgeon collected from the Boseonggang River were reared at 18.5~19.5℃ (average 19.0℃). At 5 days after hatching (total length (TL) 4.25 mm), the parasphenoid, dentary, centrum of vertebrae and clavicle began to ossify. At 22 days after hatching (TL 6.33 mm), 36 to 38 vertebrae had ossified. At 42 days after hatching (TL 16.71 mm), the majority of the skeleton had ossified. Pike gudgeon exhibited comparable characteristics to other fishes of Gobionidae throughout its development, although it displayed differences in some areas, such as the caudal skeleton and fin supports.

Changes of Activities and Isozymes of Lactate Dehydrogenase in Coreoperca herzi and Pseudogobio esocinus Acclimated to Rapid Increase of Dissolved Oxygen (급격한 용존산소량 증가에 순응한 꺽지(Coreoperca herzi)와 모래무지(Pseudogobioesocinus) 젖산탈수소효소 활성과 동위효소의 변화)

  • Cho Sung Kyu;Yum Jung Joo
    • Journal of Life Science
    • /
    • v.15 no.1 s.68
    • /
    • pp.71-79
    • /
    • 2005
  • The metabolism of lactate dehydrogenase (EC 1.1.1.27, LDH) and $C_4$ isozyme were studied in tissues of Coreoperca herzi and Pseudogobio esocinus acclimated to rapid increase of dissolved oxygen (DO). In C. herzi the LDH activity was changed $35-39\%$ in brain and liver tissues, and within $20\%$ in other tissues. The $B_4$ isozyme was increased and isozyme containing subunit C was decreased in muscle tissue. The $B_4$ isozyme was increased in heart and kidney. In P. esocinus, the LDH activity in liver tissues was largely increased to $150\%$ for 30 minute and $70\%$ in other tissues. The $A_4$ isozyme was increased in muscle and $B_4$ isozyme was increased in other tissues. Especially, the metabolism of liver tissue in P. esocinus was regulated by increasing liver-specific $C_4$ and decreasing $A_4$ isozyme. But the metabolism of eye tissue in C. herzi was regulated by decreasing LDH activity and eye-specific $C_4$ isozyme. The LDH activity and LDH isozyme in P. esocinus were largely increased than C. herzi acclimated to rapid increase of DO. And eye-specific $C_4$ and liver-specific $C_4$ isozymes played role as lactate oxidase. Therefore, the response of species acclimated to rapid increase of DO seems to be variable, perhaps due to prior exposure to environmental conditions.

Embryonic and Larval Development of Goby Minnow, Pseudogobio esocinus (모래무지(Pseudogobio esocinus)의 난발생 및 자치어 형태발달)

  • Lee, Sung-Hun;Oh, Gwang-Nam;Kim, Kwan-Seok;Oh, Yong-Seok;Kang, Kyeong-Wan;Hwang, Jae-Ho;Lee, Bae-ik;Lee, Won-Kyo;Han, Kyeong-Ho
    • Development and Reproduction
    • /
    • v.12 no.3
    • /
    • pp.283-288
    • /
    • 2008
  • The Pseudogobio esocinus were caught at Wyuleo-ri, Gyeombaek-myeon, Boseong-gun, Jeollanamdo from April to May 2003. The fishes were incubated in transparent aquarium located at the laboratory of Chonnam National University, and their embryonic and larval development were observed. The fertilized eggs were spherical, semitransparent, and adhesive, and were $1.98{\pm}0.19mm$ (n=50) in diameter. The embryo, including 31$\sim$32 myotomes, hatched through egg membrane at 164 hrs after fertilization. The newly-hatched larvae were $4.61{\pm}0.83mm$ (n=10) in total length (TL). At that moment, yolk was not absorbed, and mouth and anus were not open. Star and spot shaped melanophores were distributed on the lens, and dorsal, ventral, and caudal parts. At 42 days after hatching, larva was $16.22{\pm}0.65mm$ (n=10) in TL. Melanophores were scaterred at head, back, and side parts. Morphological features of the embryo were transferred to juvenile stage showing similar features with those of the adult fish.

  • PDF

Studies on the Dynamics of Fish Community in Wonju Stream (원주천의 어류군집 동태)

  • Choi, Jun-Kil;Byeon, Hwa-Kun;Seok, Hyung-Kun
    • Korean Journal of Ecology and Environment
    • /
    • v.33 no.3 s.91
    • /
    • pp.274-281
    • /
    • 2000
  • The dynamic of fish community was investigated in the Wonju stream from March 1998 to February 1999. The collected species during the surveyed period were 24 species belonging to eight families. Eight species (33%) of them were endemic species of Korea. According to relative abundance in the studied areas, Moroco oxycephalus (30.7%), Zacco platypus (29.6%), Carassius auratus (10%), Pseudogobio esocinus (9.4%) and Rhinogobius brunneus (3.6%) dominated. Hemibarbus longirostris, Pungtungia herzi, Squalidus japonicus coreanus, Zacco temmincki, Cobitis rotundicaudata, Silurus asotus, Silurus microdorsalis and Micropterus salmoides were rare to the areas, which occupied less than 0.1% in relative abundance. Dominant species was M. oxycephalus (Kumda-ri, Kwanseol-dong, Hanggu-dong, heongyang-ri), Z. platypus (Gaeun-dong) and C. auratus (Jusan-ri). It was revealed by the analysis of community that St. 3 was the most diverse among all study stations. Coreoleuciscus splendidus and Coreopera herzi have been assumed vanished in the Wonju stream. The species occurring in suddenly decreasing trend were Microphysogobio yaluensis, Liobagrus andersoni, Cottus poecilopus and R. brunneus. The species occurring in representative increasing trend were Cyprinus carpio, C. auratus, P. esocinus, M. oxycephalus, and Z. platypus.

  • PDF

Ultrastructure of Pseudogobio esocinus (Teleostei, Cyprinidae) Spermiogenesis and Spermatozoa (모래무지(Teleostei, Cyprinidae) 정자변형과정과 정자의 미세구조)

  • Kim, Kgu-Hwan;Kwon, Deok-Mun;Lee, Jun-Ill;Jung, Sung-Bae
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.40 no.3
    • /
    • pp.147-152
    • /
    • 2007
  • Spermiogenesis and mature spermatozoa of the Korean false minnow Pseudogobio esocinus (Cyprinidae) are described by means of scanning and transmission electron microscopy. Spermiogenesis is characterized by lateral development of the flagellum, absence of nuclear rotation, and eccentric nuclear fossa formation. The spermatozoa have a spherical head containing a nucleus with highly condensed chromatin and no acrosome. The nuclear fossa contains the proximal centriole and anterior part of the distal centriole. The midpiece is type A II and contains semi-fused mitochondria around the axoneme. However, the symmetrical distribution of 4 or more the mitochondria does not follow a general pattern of the cyprinid spermatozoa. Cytoplasmic vesicles in the midpiece are common in Cypriniformes and some of Siluriformes and Characiformes spermatozoa.

The Ichthyofauna and Dynamics of the Fish Community in Miho Stream, Korea (미호천의 어류상과 어류군집 동태)

  • Son, Yeong-Mok;Byeon, Hwa-Kun
    • Korean Journal of Ichthyology
    • /
    • v.17 no.4
    • /
    • pp.271-278
    • /
    • 2005
  • The ichthyofauna and fish community were studied in Miho Stream from May 2004 to August 2005. During the surveyed period 41 species belonging to 10 families were collected. Twelve of the species (29.3%) are endemic to Korea. Exotic fishes were Carassius cuvieri, Lepomis macrochirus, and Micropterus salmoides. Dominant species was Zacco platypus (54.7%). Comparing the present result with that of Son (1982), the indices of diversity,evenness and richness were lower and the dominance index was higher; Lampetra reissneri, Anguilla japonica, Acheilognathus macropterus, Coreoleuciscus splendidus, Pseudopungtungia nigra, Gobiobotia naktongensis, Lefua costata, Pseudobagrus koreanus, Monopterus albus and Iksookimia choii did not appear; Carassius auratus, Rhodeus uyekii, Rhodeus notatus, Acheilognathus lanceolatus, Pseudogobio esocinus, Hemibarbus longirostris, Microphysogobio yaluensis, Cobitis lutheri, Iksookimia koreensis, Pseudobagrus fulvidraco, and Rhinogobius brunneus, remarkably decreased, and Z. platypus, Pseudorasbora parva, and Squalidus gracilis majimae increased.

Fish Fauna and Community Structure in the Mid-Upper Region of the Seomjin River (섬진강 중.상류 수계의 어류상과 군집구조)

  • Jang, Sung-Hyun;Ryu, Hui-Seong;Lee, Jung-Ho
    • Korean Journal of Ecology and Environment
    • /
    • v.42 no.3
    • /
    • pp.394-403
    • /
    • 2009
  • The fish fauna and community structures were investigated at 15 stations in the midupper region of the Seomjin River watershed from August in 2008 to April in 2009. During the studying period, 42 species belonging to 11 families were collected. Dominant family in the all sites was Cyprinidae (28 species, relative abundance: 66.7%) during the study. Total of 42 species (primary freshwater: 40 species, peripheral freshwater: 2 species) were found with the primary freshwater fishes being the highest. Dominant species was Zacco platypus (22.3%), and subdominant species was Coreoferea herzi (10.8%). Also, Pseudogobio esocinus (9.0%) and Carassius auratus (5.8%) were numerous. There were 17 Korean endemic species (40.5%), including Rhodeus ocellatus, Acheilgnathus koreensis, A. somjinensis, Coreoleuciscus splendidus and Sarcocheillichthys variegatus wakiyae. Among them, Acheilgnathus somjinensis was identified for designation required to protect under a wildlife category. In conclusion, we could define that the Seomjin River watershed has a very good water conditions as habitat of fish.

On the Pharyngela Bones and Their Teeth in Korean CYPRINIDS Fishes (한국산 잉어과 어류의 인두골과 인두치(제 1 보))

  • 양홍준
    • The Korean Journal of Zoology
    • /
    • v.6 no.1
    • /
    • pp.15-20
    • /
    • 1963
  • The characteristics of the pharyngeal bones and their teeth in Korean Cyprinids fishes, as a rule, are summarized as follow : 1. The materials 13 genera and 17 species from Nak Dong River. 2. Breadth of bone about 2.8(Cyprinus carpio) to 4.9(Culter brevicauda) in bone length. Length of bone about 8.5(Carassius carassius ) to 20.2 (Pseudogobio esocinus ) in body length. Breadth of pitted surface about 66%(Carassius carassius) to 85%(Acanthorhodeus asmussi ) of breadth of bone. 3. The position and distinct ratio of anterior angle correlated. 4. Posterior limb always longer than anteriro, but posterior edentulous process not shorter than anterior's or equal. 5. Zacco platypus with pitted surface on anterior limb , but Z.temmincki without it. 6. Teeth one to three rowed in accordance with the differences of each genus : a. one rowed genera ..... Carassius , Pseudorasbora, Sarcocheilichthys (Sakiyae), Pungtungia, Acheilognathus, Acanthorhodeus. b. two rowed genera ........ Pseudogobio, Gnathopogon, Tribolodon. c. Three rowed genera .... Cyprinus, Hemibarbus, Zacco , Culter 7. Teeth of main (primary ) row with four or five teeth is symmetrized exceptive three genera (Tribolodon, Zacco, Culter). 8. Grinding surface and bill type correlated. 9. In Acheilognathus, A.Yamatsutae have one or two indistinct serration but each individual is unlike.

  • PDF

A Study on the Residual Microplastics in Freshwater and Fishes in the Geum River Watershed (금강수계 담수와 어류체내 잔류미세플라스틱 연구)

  • Kim, Nam-Shin;Yoon, Ju-Duk;Lee, Seung-Eun;Park, Young-Joon;Woo, Seung-Hyun
    • Korean Journal of Ecology and Environment
    • /
    • v.52 no.1
    • /
    • pp.28-39
    • /
    • 2019
  • This study was conducted to elucidate microplastics detection of freshwater ecosystems in Geum river. Samples are collected at 6 points in freshwater, 5 points in fishes. Freshwater was sampled 100 L per each points and fish species were Opsariichthys uncirostris amurensis, Hemibarbus labeo, Pseudogobio esocinus, Zacco platypus, Micropterus salmoides and Cyprinus carpio. FTIR analyis was adopted to identify microplastic types. Extracted microplastics were PES (polyester), PE (polyethylene), PP (polypropylene), PET (polyethylene terephthalate), PVC(Polyvinyl chloride) in freshwater, and PES, PE, PP, PET, PVC in fishes. Our results were expected to be used basic research information for further study in microplastics of freshwater ecosystems.