• Korean Journal of Microbiology
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• v.54 no.1
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• pp.69-70
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• 2018

Erwinia pyrifoliae is a Gram-negative bacterium causing black shoot blight in apple and Asian pear trees. E. pyrifoliae strain EpK1/15 was isolated in 2014 from an apple twig from the Pocheon, Gyeonggi-do, South Korea. In this study, we report the draft genome sequence of E. pyrifoliae EpK1/15 using PacBio RS II platform. The draft genome is comprised of a circular chromosome with 4,027,225 bp and 53.4% G + C content and a plasmid with 48,456 bp and 50.3% G + C content. The draft genome includes 3,798 protein-coding genes, 22 rRNA genes, 77 tRNA genes, 13 non-coding RNA genes, and 231 pseudo genes.

• The Korean Journal of Ecology
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• v.26 no.4
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• pp.203-208
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• 2003

Losses in the dry weight of leaf litter from six tree species were studied during 16 months on the forest floor in temperate deciduous forest of Mt. Cheonma in the vicinity of Seoul in Korea by using litter bag method. The decomposition rate of each leaf litter varies with each species. After 16 months elapsed, the leaf litter of Acer pseudo-sieboidianum showed the highest decomposition constant (0.82) as Olson´s decomposition constant, while that of Pinus densiflora showed the lowest decomposition constant (0.33). The decomposition constant of Quercus mongolica, Q. serrata, Betula ermani and Carpinus laxiflora showed 0.43, 0.37, 0.66 and 0.75, respectively. The decomposition constant of leaf litter was considered with temperature and precipitation which accumulated daily during each term of litter bag collection. The decomposition constant of leaf litter showed closely positive correlation with daily accumulative temperature and precipitation. The relationships between decomposition constant and the daily accumulative temperature and precipitation at each period of litter bag collection were analyzed through multi-regression analysis. The correlation coefficients as a result of multi-regression analysis in Q. mongolica, Q. serrata, P densiflora, B. ermani, C. laxiflorais and A. pseudo-sieboldianum were 0.83, 0.81, 0.69, 0.77, 0.77 and 0.62, respectively. The precipitation showed higher effect, about 10 times, on the leaf litter decomposition than the daily accumulative temperature.

• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.838-844
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• 2001

The enzyme Fuc aldolase from Methanococcus jannaschii that catalyzes the aldol condensation of DHAP and L-lactaldehyde to give fuculose-1-phosphate was inactivated by DEP. The inactivation was pseudo first-order in the enzyme and DEP, which was biphasic. A pseudo second-order rate constant of 120$M^{-1}min^{-1}$ was obtained at pH 6.0 and $25{\circ}C$. Quantifying the increase in absorbance at 240nm showed that four histidine residues per subunit were modified during the nearly complete inactivation. The statistical analysis and the time course of the modification suggested that two or three histidine residues were essential for activity. The rate of inactivation was dependent on the pH, and the pH inactivation data implied the involvement of the amino acid residue with a $pK_a$ value of 5.7. Fuc aldolase was protected against DEP inactivation by DHAP, indicating that the histidine residues were located at the active site of Fuc aldolase. DL-Glyceraldehyde, as an alternative substrate to L-lactaldehyde, showed no specific protection for the Fuc aldolase.

• Journal of Environmental Science International
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• v.18 no.5
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• pp.527-538
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• 2009

A village grove is the one fostered and protected for a district's traditional life near the vicinity of a ruderal village. This research is done to grasp potential natural vegetation, to use a basis data needed for its management and preservation as well as restoration project by examining the vegetation and flora targeting a village grove of the southern district of Gyeonggi-do which has been seriously damaged subsequent to a rapidly progressing city development. According to the survey, there appeared an outgrowth flora of Pteridophyta consisting of 69 families, 136 genera, 20 varieties, 3 forms, 170 species-totalling to 193 taxa. Urbanization index was revealed to 4.55% and naturalization rate 6.74%. In life-cycle pattern, perennials were found to show the highest distribution rate; in infiltration-multiplication strategies, human wave tactics type was found to be the highest 51%, consisting of 98 species, and naturalization rate also was revealed to be 6.74%. The vegetation unit was divided into a total of 11 patterns, such as a community of Pinus densiflora community, Pinus densiflora-Pinus rigida community, Pinus densiflora-Castanea crenata community, Pinus rigida community, Pinus rigida-Castanea crenata community, Pinus rigida-Quercus acutissima community, Castanea crenata community, Castanea crenata-Quercus acutissima community, Quercus acutissima community, Quercus acutissima-Robinia pseudo-acacia community and Robinia pseudo-acacia community. Quercus acutissima community is of a community type dominated by Quercus acutissima, in place of Pinus densiflora community selected by natural succession.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.366-371
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• 1998

To investigate the structure of Korean native species, Abies koreana forest in subalpine zone of Mt. Halla, 20 plots ($10{\times}10m$) set up with random sampling method were surveyed. The forest on subalpine zone in Mt. Halla was Abies koreana - Taxus cuspidata - Sasa quelpaertensis community. High positive correlations were shown between Quercus ${\times}$ grosseserrata and Carpinus laxiflora; Carpinus laxiflora and Acer pseudo-sieboldianum, Ilex crenata; Taxus cuspidata and Rhododendron mucronulatum var. ciliatum, Prunus padus, and high negative correlations were shown between Abies koreana and Quercus ${\times}$ grosseserrata; Abies koreana and Acer pseudo-sieboldianum; Sasa quelpaertensis and Prunes sargentii. Vigor of Abies koreana was depressed, 8.11% of total number of Abies koreana investigated were dead. DBH of dead individuals ranged mainly 10-30 cm.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.633-640
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• 2005

The purified xylanase from Bacillus pumilus TX703 was modified with various chemical modifiers to determine the active sites of the enzyme. Treatment of the enzyme with group-specific reagents such as carbodiimide or N-bromosuccinimide resulted in complete loss of enzyme activity. These results assumed that these reagents reacted with glutamic acid or aspartic acid and tryptophan residues located at or near the active site. In each case, inactivation was performed by pseudo first-order kinetics. Inhibition of enzyme activity by carbodiimide and W-bromosuccinimide showed non-competitive and competitive inhibition type, respectively. Addition of xylan to the enzyme solution containing N-bromosuccinimide prevented the inactivation, indicating the presence of tryptophan at the substrate binding site. Analysis of kinetics for inactivation showed that the loss of enzyme activity was due to modification of two glutamic acid or aspartic acid residues and single tryptophan residue.

• Korean Journal of Environmental Biology
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• v.27 no.3
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• pp.314-322
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• 2009

Vegetation, environmental characteristics and soil analysis of Megaleranthis saniculifolia Ohwi habitats were investigated to propose the basal data for conservation and restoration. M. saniculifolia was distributed around an altitude of 770~1,440 m with an inclination of $0{\sim}20^{\circ}$, and mostly formed discontinuous populations in northern part of valley. The vascular plants from 78 quadrates of 11 habitats were identified 111 taxa. Importance value of M. saniculifolia was 27.05%, and highly ranked 5 species such as Veratrum oxysepalum (5.67%), Corydalis turtschaninovii (5.32%), Aruncus dioicus var. kamtschaticus (4.35%), Meehania urticifolia (4.06%) and Anemone koraiensis (3.91%) were considered to be an affinity with M. saniculifolia. Dominant species of woody plants in 78 quadrates were represented as Cornus controversa, Juglans mandshurica, Quercus mongolica, Acer pseudo-sieboldianum and A. mono in tree (B1) layer, A. pseudo-sieboldianum and Carpinus cordata in subtree (B2) layer, Deutzia glabrata and Prunus padus in shrub (S) layer. Average species diversity was 1.16, and dominance and evenness were found to be 0.12 and 0.81, respectively. Average field capacity was 26.41%, and the organic matter and soil pH were 7.83% and 5.83. Correlation coefficients based on environmental factors, vegetation and soil analysis were showed that the positive correlations between slope degrees and pH, slope degrees and organic matter, dominance and importance value, species diversity and richness, whereas between species diversity and dominance, and importance value and dominance were showed negative correlations.

• BMB Reports
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• v.50 no.5
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• pp.235-236
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• 2017

The circadian clock is an internal system that is synchronized by external stimuli, such as light and temperature, and influences various physiological and developmental processes in living organisms. In the model plant Arabidopsis, transcriptional, translational and post-translational processes are interlocked by feedback loops among morning- and evening-phased genes. In a post-translational loop, plant-specific single-gene encoded GIGANTEA (GI) stabilize the F-box protein ZEITLUPE (ZTL), driving the targeted-proteasomal degradation of TIMING OF CAB EXPRESSION 1 (TOC1) and PSEUDO-RESPONSE REGULATOR 5 (PRR5). Inherent to this, we demonstrate the novel biochemical function of GI as a chaperone and/or co-chaperone of Heat-Shock Protein 90 (HSP90). GI prevents ZTL degradation as a chaperone and facilitates ZTL maturation together with HSP90/HSP70, enhancing ZTL activity in vitro and in planta. GI is known to be involved in a wide range of physiology and development as well as abiotic stress responses in plants, but it could also interact with diverse client proteins to increase protein maturation. Our results provide evidence that GI helps proteostasis of ZTL by acting as a chaperone and a co-chaperone of HSP90 for proper functioning of the Arabidopsis circadian clock.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.49 no.12
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• pp.679-684
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• 2000

Closing switch, key component of pulsed power system, is constructed simply and used frequently due to the easy control and manufacture of one. The kind of one are spark-gap, triggered vacuum switch, pseudo-spark switch and INPIStron. But the electrode of spark gap switch is damaged with the hot spot by Z-pinch and then the life of one become short. INPIStron with inverse pinch effect has long life but it is difficult trigger system to provide uniform discharge between cathode and anode. In this paper, the design and manufacturing of INPIStron with gas puffing trigger method in order to supply uniform discharge inter-electrode and the performance of the developed INPIStron applied to 500[kA]-2[MJ] pulsed power system is presented.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.4
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• pp.225-230
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• 2002

Experiments on deactivation kinetics of immobilized lipase enzyme from Candida cyl-indracea were performed in stirred bath reactor using rice bran oil as the substrate and temperature as the deactivation parameter. The data were fitted In first order deactivation model. The effect of temperature on deactivation rate was represented by Arrhenius equation. Theoretical equations were developed based on pseudo-steady state approximation and Michaelis -Menten rate expression to predict the time course of conversion due to enzyme deactivation and apparent half-life of the immobilized enzyme activity in PFR and CSTH under constant feed rate polity for no diffusion limitation and diffusion limitation of first order. Stability of enzyme in these continuous reactors was predicted and factors affecting the stability were analyzed.