• Horticultural Science & Technology
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• v.33 no.2
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• pp.242-250
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• 2015

Insertional mutagenesis induced by T-DNA or transposon tagging offers possibilities for analysis of gene function. However, its potential remains limited unless good methods for detecting the target locus are developed. We describe a PCR technique for efficient identification of DNA sequences adjacent to the inserted T-DNA in a higher plant, Chinese cabbage (Brassica rapa ssp. pekinensis). This strategy, which we named variable argument thermal asymmetric interlaced PCR (VA-TAIL PCR), was designed by modifying a single-step annealing-extension PCR by including a touch-up PCR protocol and using long gene-specific primers. Amplification efficiency of this PCR program was significantly increased by employing an autosegment extension method and linked sequence strategy in nested long gene-specific primers. For this technique, arbitrary degenerate (AD) primers specific to B. rapa were designed by analyzing the Integr8 proteome database. These primers showed higher accuracy and utility in the identification of flanking DNA sequences from individual transgenic Chinese cabbages in a large T-DNA inserted population. The VA-TAIL PCR method described in this study allows the identification of DNA regions flanking known DNA fragments. This method has potential biotechnological applications, being highly suitable for identification of target genomic loci in insertional mutagenesis screens.

• Journal of Korea Multimedia Society
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• v.7 no.8
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• pp.1131-1144
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• 2004

In this paper, we investigate a problem with an e-learning system for e-business environments and introduce the solving method of the problem. To be more accurate, existing Web-hosted and ASP (Application Service Provider)-oriented service model is difficult to cooperate and integrate among the different kinds of systems. So we have produced sharable and reusable learning object, they have extracted a principle from pedagogical designs for units of reuse. We call LIO (Learning Item Object). This modeling makes use of a constructing for XML Web Services. So to speak, units of reuse from pedagogical designs are test tutorial, resource, case example, simulation, problem, test, discovery and discussion and then map introduction, fact, try, quiz, test, link-more, tell-more LIO learning object. These typed LIOs are stored in metadata along with the information for a content location. Each one of LIOs is designed with components and exposed in an interface for XML Web services. These services are module applications, which are used a standard SOAP (Simple Object Access Protocol) and locate any computer over Internet and publish, find and bind to services. This guarantees the interoperation and integration of the different kinds of systems. As a result, the problem of e-learning systems for e-business environments was resolved and then the power of understanding about learning objects based on pedagogical design was increased for learner and instruction designers. And organizations of education hope for particular decreased costs in constructing e-learning systems.

• KDI Journal of Economic Policy
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• v.33 no.4
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• pp.85-118
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• 2011

This paper uses the horizontal regulation system as the base analysis framework. The study clearly defines the regulatory goals of the followings: the horizontal cross-ownership regulations on program provider (PP) and platform provider, the vertical regulation on cross-ownership between PP and platform operator, the regulation on cross-ownership of program provider by terrestrial broadcasting company, and the regulation on cross-ownership between terrestrial broadcasting company and platform provider. Then, by analyzing the conformity between goals and criteria of regulations and the adequacy of the regulation level according to regulatory purposes, this paper examines the justifiability of each regulation and extracts improvement measures that suite regulatory purposes. This analysis finds following appropriate measures: replacing the horizontal cross-ownership regulation on PP with conduct regulations, such as designating major broadcasting programs or replacing the current criterion of cross-ownership regulation from sales to the audience market share; reshaping the horizontal cross-ownership regulation on platform provider so that system operator (SO), satellite broadcaster and Internet protocol television (IPTV) operator would be applied by the same regulation based on the number of subscribers of pay television services; and discontinuing other cross-ownership regulation. In this way, the study shows that with appropriate regulations on cross-ownership of PP, there would be no need for additional regulation on vertical integration between PP and platform operator. On the other hand, given that the regulation on terrestrial broadcasting cross-ownership of PP could be justified only by regulatory purpose of the protection of the diversity of public opinions, it would be desirable to replace the current criteria of the number of PPs with the criteria of the audience market share. Lastly, the study shows that when platform operator is targeted by the cross-ownership regulation based on the number of subscribers of pay television services, the regulation on cross-ownership between terrestrial broadcasting company and platform provider should be replaced with conduct regulations, such as designating must-offer channels and major broadcasting programs.

• Spatial Information Research
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• v.12 no.2
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• pp.151-163
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• 2004

This study aims to develop a standard protocol by which users can exchange data information efficiently between KOSDIC and GIS. The ultimate goal from this study is to propose a data exchange format using KOSIC. The background of this study is that GIS industry sector is vigorously developing linked to spatial information construction such as UIS, LIS, EIS, AM/FM through supporting by regional government institution. According to do, the format of data transformation becomes major issue. GIS data can easily transfer to CAD version but when CAD data convert to GIS format (DWG->DXF->SHP) cannot get high success rate due to errors and problems. In addition, CAD data feature is made from oriental structure which is made of point and line faeture so that GIS system is impossible to data transformation. In the reason, the biggest problem is discordance of symbolization in integration and standard of data format. By investigation the usability of this study, conclusion are drawn efficient alternative plan in data transformation. Though this wort we will be able to provide a better transformation of data conversion between CAD format and GIS format. Accordingly, it is of important to enrich the integrated construction information infrastructure, which accommodates ever-changing constructions environment.

• Journal of KIISE:Computing Practices and Letters
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• v.8 no.5
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• pp.607-617
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• 2002

IETF has proposed CNRP that is a protocol exchanging CNRP objects between server and client for resolving a common name to URIs of the desired Internet resouces. CNRP enables users to access various services via the integrated interface, to easily get the desired resources, and to reuse the results not as the data but as the information. Whereby these advantages, CNRP will be generally used for the integration of the various Internet services or the applications where the resolution of common name is needed. But, CNRP specification doesn't describes the practical implementation method for server and client. Though a few prototype systems are developed in some researches using CNRP, they are not enough to be generally used for the various Internet applications because they doesn't include all objects specified in CNRP specification or they construct systems with one server. So, this Paper proposes the practical implementation method for CNRP server/client through analyzing and solving the problems occurred when implementing them, and implements all objects specified in CNRP specification. This paper also verifies the feasibility of the proposed method by developing the prototype system of the company name resolution service using the CNRP server/client implemented in this study. The CNRP server/client implemented in this paper are used to develop various CNRP application systems.

• 한국정보통신설비학회:학술대회논문집
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• 2008.08a
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• pp.211-217
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• 2008

Recently, there are many efforts to support seamless mobility in 802.11 WLANs using IP Layer mobility protocols. The IP layer mobility protocols are the most efficient mechanism to guarantee the service session continuity when IP subnet is changed during handover. Even if the IP layer mobility protocols are quite efficient, the feature of the protocols that had been designed to consider only L3 layer makes it difficult to improve the performance of hand over more and more. Nowadays, to overcome this limitation of IP mobility protocols, many researchers have worked on the mobility protocols integration of different layers (e.g., L2 layer). In this paper, we propose the enhanced Proxy MIPv4 to minimize the latency of handover using MIH protocol in 802.11 WLANs. The proposed mechanism minimizes the latency of authentication by exchanging security keys between Access Routers during handover. Moreover, it also minimizes packet losses by Inter-AP Tunneling and data forwarding.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.11
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• pp.2083-2090
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• 2006

In an proposed Context-Aware SOA(Service Oriented Architecture) based OSGi(Open Service Gateway initiative) platform, Service provider manages relative kinds of services in an integrative basis from various sensors, puts each service in a SOAP (Simple Object access Protocol) message, and register thorn to the UDDI(Universal Description Discovery and Integration) server of service registry, service requester retrivel the specified kinds of services and call them to service provider. Recently most context-aware technologies for ubiquitous home network are mainly putting emphasis on RFID/USN and location-based technology. Because of this, service-oriented architecture researches have not been made enough. Under the environment of an OSGi service platform, various context-aware services are dynamically mapping from various sensors, new services are being offered for the asking of users, and existing services are changing. Accordingly, the data sharing between services provided, management of service life cycle, and the facilitation of service distribution are needed. Taking into considering all these factors, this study has suggested an Context-Aware SOA based eclipse SOA Tools Platform using OSGi platform that can transaction throughtput of more than 546 TPS of distributional Little's Law from ATAM(Architecture Tradeoff Analysis Method) while remaining stable other condition.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.1
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• pp.185-191
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• 2015

This paper suggest the safety class communication board in order to design the safety network of the nuclear safety class controller. The reactor protection system use the digitized networks because from analog system to digital system. The communication board shall be provided to pass the required performance and test of the safety class in the digital network used in the nuclear safety class. Communication protocol is composed of physical layer(PHY), data link layer(MAC: Medium Access Control), the application layer in the OSI 7 layer only. The data link layer data package for the cyber security has changed. CRC32 were used for data quality and the using one way communication, not requests and not responses for receiving data, does not affect the nuclear safety system. It has been designed in accordance with requirements, design, verification and procedure for the approving the nuclear safety class. For hardware verification such as electromagnetic test, aging test, inspection, burn-in test, seismic test and environmental test in was performed. FPGA firmware to verify compliance with the life-cycle of IEEE 1074 was performed by the component testing and integration testing.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.53-65
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• 2020

Here, we describe an efficient and rapid protocol for the micropropagation of Thymus pallidus Cosson ex Batt., a very rare medicinal and aromatic plant in Morocco. After seed germination, we tested the effect of different macronutrients, cytokinins alone or in combination with gibberellic acid (GA₃) or auxins, on T. pallidus plantlet growth. We found that Margara macronutrients (N₃₀K) had the best effect on the in vitro development of the plantlets. The addition of 0.93 μM/L 1,3-diphenylurea (DPU), 0.46 μM/L adenine (Ad), and 0.46 and 0.93 μM/L kinetin (Kin) resulted in the best shoot multiplication and elongation. In addition, the combination of 0.46 μM/L Kin, DPU, or Ad with gibberellic acid, in particular, 0.46 μM/L Ad + 0.58 μM/L GA₃ and 0.46 μM/L Kin + 1.15 μM/L GA₃, led to better bud and shoot multiplication. Moreover, the integration of the combinations of 0.46 μM/L Kin and auxins, namely 0.46 μM/L Kin + 2.85 μM/L indole-3-acetic acid (IAA), 0.46 μM/L Kin + 2.85 or 5.71 μM/L indole-3-butyric acid (IBA), and 0.46 μM/L Kin + 0.3 or 0.57 μM/L 1-naphthaleneacetic acid (NAA), in the culture medium led to better root development and optimized aerial growth. Finally, the in vitro plants from the medium containing N₃₀K + 0.46 μM/L Kin + 2.85 μM/L IAA were successfully acclimatized; these plants served as a source for repeating in vitro culture.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.5
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• pp.115-123
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• 2009

Integrated information to water quantity and quality is essential for planning water resources management as well as operating water-related infrastructures. Because data collection process including monitoring and maintenance is separated in different governmental agencies in Korea, integrating quantity and quality may provide effective and better management implementation. In this study, a number of suggestions regarding integration of water monitoring were concluded in terms of technological, legal and institutional implications. First, it is necessary to discuss national water monitoring plan, national water information management plan, agreement of standard terms of monitoring between ministries, and to revise the law(river law and water quality management law). Present stations for water monitoring should be used for both of quantity and quality monitoring. If station is newly installed or relocated, it is better that one single agency maintain monitoring frequency and data management as well. In addition, a monitoring protocol need to be agreed by each of parties. In order to develop integrated monitoring system, quality assurance of the collected data should be properly maintained. Since many purposes haven been concerned using of data analysis and assessment so far, it may not be easy to integrate water quantity and quality monitoring in a short period. However, the alternatives including enhancing institutional regulations and programs, advanced technology may promote an efficient integrated water monitoring.