• Advances in concrete construction
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• 제16권5호
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• pp.243-254
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• 2023

Microtubules in the cell are influenced by internal and external stimulation and play an important part in conveying protein substances and in carrying out medications to the intended targets. Waves are produced during these functions and in order to control the biological cell functions, it is important to know the wave velocities of microtubules. Owing to cylindrical shell shaped and mechanically elastic and orthotropic, cylindrical shell model based on gradient elasticity theory has been used. Wave velocities of the protein microtubule are carried out by considering Love's thin shell theory and Navier solution. Also the effect of size parameter and other variables on the results are investigated.

• KSBB Journal
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• 제30권6호
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• pp.291-295
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• 2015

The limited productivity of natural shell matrix proteins has hampered the investigation of their biochemical properties and practical applications, although biominerals in nature obtained by organic-inorganic assemblies have attractive mechanical and biological properties. Here, we prepared a vector for the expression of a fusion protein of a shell matrix protein from Pinctada fucata (named as GRP_BA) with the GRGDSP residue. The fusion protein of BA-RGD was simply produced in E. coli and purified through sequential steps including the treatment with $CaCl_2$ and EDTA solution for cell membrane washing, mechanical cell disruption and the application of non-ionic surfactant of Triton X-100 for BA-RGD inclusion body washing. The production yield was approximately 60 mg/L, any other protein band was not observed in SDS-PAGE and it was estimated that above 97% endotoxin was removed compared to the endotoxin level of whole cell. This study showed this simple and easy purification approach could be applied to the purification of BA-RGD fusion protein. It is expected that the protein could be utilized for the preparation of biominerals in practical aspects.

• 한국염색가공학회지
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• 제11권2호
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• pp.55-63
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• 1999

The purpose of this study was to investigate the purification of chitin and chitosan for textile finishing agent from crab shell. Weight loss rate(removing Ca and protein), degree of deacetylation, solubility and MIC(Minimum growth inhibitory concentration) value of chitosan and molecular weight of the treated crab shell were measured. The results of this study were as follows : 1) Weight loss rate(removing Ca) of crab shell treated with HCI increased with the concentration of HCI and treatment time, but it became constant over 60 min. of treatment time. 2) Weight loss rate(removing protein) of crab shell treated with NaOH(0.5N∼2N) increased with the concentration of NaOH and treatment temperature and time, but it became constant above loot of temperature and over 200 min. of treatment time. 3) Degree of deacetylation of chitin treated with NaOH increased with the concentration of NaOH(40∼60％), but molecular weight decreased and thus MIC value increased. 4) Concentration of acetic acid should be above 0.3％ to dissolve chitosan easily. Solubility for chitosan was the highest with formic acid, and the next was acetic acid, hydrochloric acid, lactic acid and sulfuric acid in order.

• 한국식품영양과학회지
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• 제22권6호
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• pp.763-770
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• 1993

To investigate the quality changes during frozen storage, top shell, Omphalius pfeifferi capenteri, was stored at -18$^{\circ}C$, -$25^{\circ}C$ and -3$0^{\circ}C$ immediately after shelling and water holding capacity, protein composition and histological features were examined with the lapsed period of the storage. During the storage period, amount of free drip was increased with higher frozen temperature and longer frozen period, but with the longer storage period, the lower water holding capacity was observed. The extractability and composition of muscle protein, sarcoplasmic protein and stroma protein were rather stable regardless of frozen temperature and frozen storage period. However, the extractability of myofibrillar protein was decreased with higher frozen temperature and longer frozen storage period. On the changes of muscle tissue structure, following points were observed. 1) In the muscle tissue structure of fresh sample, fine muscle fiber was closely distributed all over the tissue regardless of cross and longitudinal section. 2) In tissue structure under frozen state, it was observed that ice crystals apparently grew with the higher storage temperature. Empty spaces between muscle bundles which wee formed by aggregations of muscle fiber were observed after 3 months storage at -18$^{\circ}C$ . 3) Tissue structure in thawed state was restored satisfactorily after 1 month storage regardless of storage temperature. After 3 months storage at -3$0^{\circ}C$, muscle tissue was well restored, but at -18$^{\circ}C$, empty spaces were apparent due to incomplete restoration.

• Fisheries and Aquatic Sciences
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• 제4권1호
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• pp.18-24
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• 2001

Seasonal content changes of the three key nutrients for organisms, protein, lipid and glycogen, were analyzed for a whole year to delineate the seasonal energy strategy in pen shells, Atrina pectinata. Two metabolically important organs, the visceral mass and the posterior adductor muscle, were examined. Protein in the visceral mass rose in April and stayed at the level until June followed by the constant minimum value between August and November. The protein contents in the posterior adductor muscle increased sharply in April and again in July, followed by a gradual decline thereafter. Total lipid contents in the visceral mass gradually increased between January and May, and then slowly decreased until September since which a new weak increase was noticed. Lipid levels in the adductor muscle rapidly dropped in June and July. Glycogen contents in the visceral mass rapidly increased between February and June, followed by a drastic drop in July. This reduced visceral glycogen level was maintained up to September, and a gradual reduction ensued. Glycogen contents in the adductor muscle steadily but markedly increased from April reaching the maximum in August, and then slowly declined thereafter. These results suggest that an accelerated protein and lipid synthesis occurs in the gonad when the pen shell undergoes the ripe stage of gametogenesis, but the levels of these two nutrients decrease on spawning. With this gonadal process, regular protein synthesis and lipid storage in the posterior adductor muscle are temporarily arrested. The most important nutrient reserves that support gonad developmental cycles in a long term seem to be glycogen of the posterior adductor muscle.

• Journal of Nutrition and Health
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• 제45권5호
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• pp.429-436
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• 2012

본 연구는 폐기되는 홍게껍질의 단백질을 활용하기 위하여 홍게껍질 분말에 상업적으로 사용하는 6가지 protease를 단일 또는 혼합처리하여 획득한 가용성 가수분해물의 분자량 및 아미노산 조성 등을 비교 분석하여 기능성 소재 및 보충제로서의 활용 가능성을 확인하고자 하였다. 단일효소 6종 중 단백질 분해능은 Biuret assay과 280 nm assay 및 $^{\circ}Brix$로 분석한 결과 Protease A (PA)가 현저하게 높았다. 단일효소 처리로 생성된 가수분해물의 단백질 분자량은 150 kDa 이하의 밴드를 형성하였다. 단일효소 중 단백질 분해능이 가장 강력한 PA의 최적 가수분해 조건을 선정하여 PA에 Protamex (P), Flavourzyme (F), Alcalase (A), Protease M (PM)과 Protease A (PA)를 1대 1의 비로 혼합 (PA + P, PA + PM, PA + F, PA + A)하여 GC로 아미노산 조성을 확인하였다. 총 아미노산 함량은 PA + P 247.411 mg/g > PA + F (206.442 mg/g) > PA + A (133.385 mg/g) > PA + PM (59.131 mg/g) > PA (54.875 mg/g)의 순으로 혼합효소처리가 단일효소처리에 비해 높은 것을 확인할 수 있었다. 혼합효소처리 한 홍게껍질의 아미노산 조성은 사용 효소에 따라 다소 차이가 있었다. 주로 phenylalanine, glycine, alanine, leucine의 함량이 높았으며 tyrosine, cystine은 검출되지 않았다. 쌀을 주식으로 하는 우리나라 사람들에게 부족되기 쉬운 필수 아미노산인 lysine, phenylalnine, leucine, isoleucine의 함량이 높아 아미노산 보충효과를 기대할 수 있을 것으로 보인다.

• 한국환경농학회지
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• 제24권2호
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• pp.185-190
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• 2005

일부 농가에서 이용하고 있는 기능성 쌀 생산 농자재인 게 껍질, 견운모 및 숯 분말의 시용이 벼의 생육, 수량 및 쌀의 품질에 미치는 영향을 구명하고자 본 실험을 실시하였다. 게 껍질 분말 시용구는 다른 처리에 비하여 분얼수, 이삭수, 엽면적지수, 식물체의 질소함량을 증가시켰으나 등숙율은 낮았고, 백미수량은 차이가 없었다. 미질은 게 껍질 분말 시용구에서 완전미 비율과 Toyo 식미치는 낮았고, 분상질미 비율, 단백질 함량은 높았다. 견운모와 숯 분말 처리는 벼의 생육, 수량 및 수량구성요소, 쌀의 품질은 무처리와 차이가 없었고, 단백질 함량는 무처리보다 높았으나 Toyo 식미치는 낮았다.

• 한국축산식품학회지
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• 제22권3호
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• pp.267-273
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• 2002

본 연구는 난각막(egg shell membrane)에 keratinase를 생성하는 Bacillus licheniformis를 배양하여 제조한 효소분해 난각막분해물(enzyme-degradation egg shell membrane hy-drolysate, EESMH)과 IN NaOH-Ethanol로 분해한 알칼리분해 난각막분해물(alkalic-degradation egg shell membrane hy-drolysate, AESMH)을 제조하여 식품소재로 활용하고자 성분분석 및 분해물의 유화성, 거품형성력, 항산화력 등의 식품학적 기능을 확인하였다. 즉, EESMH의 생산수율은 약 15% 였고 AESMH의 경우 약 70%의 수율로 가수분해물이 생성됐으며 아미노산 분석 결과 EESMH와 AESMH의 아미노산 구성은 주성분이 aspartic acid, glutamic acid, cystine으로 큰 차이가 없었으나 EESMH의 경우 histidine이 18.69%로 AESMH의 2.56%에 비해 6배 정도 함량이 높게 나타났다. 단백질의 기능적 특성 측면에서 용해성은 EESMH와 AESMH 모두95%이상의 높은 용해성을 나타내었다. 유화 활성의 경우 EDESDBB가 더 효과가 있었으며, 유화 안정성도 EESMH가 AESMH보다 약 8% 안정하였다. 거품 형성력은 AESMH가 EESMH보다 모든 pH범위에서 약 2배 정도 높았으며 안정성도 우수하였다. 항산화력의 경우AESMH가 항산화력이 높은 것으로 확인하였고 EESMH의 경우 전체적으로 매우 약하게 나타났다.

• Journal of Nutrition and Health
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• 제21권2호
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• pp.122-128
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• 1988

This nutritional survey was conducted from September 17 to October 6, 1984, in order to investigate the state of the nutritional intakes of perple living Gapoe-Dong, coastal area of Korea. This survey covered a total of forty houses. The method of this research was based on the interiview-survey with questinaires. All of the food taken daily by the people are all weighed before cooking ; the rest of food after mealing are also weighed. Thus the amount of food taken by people can be come out. The results were summarized as follows; 1) The average intake of all nutrients were below the RDA, especially calorie, vitamin B2, calcium and iron were insufficient. The protein intake was only 55.2g but the contribution of animal protein to total protein was adequate(38%). It was provided mostly by fish and shell-fish. 2) Chief food sources were rice, vegetables, fish and shell-fish, and milk, eggs, oil, potato, and fruits were insufficient. 3) The level of education and the number of family were significantly correlated to the nutrientintake, especially correlation between family-size and colorie, calcium intake were significant.

• 한국양식학회지
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• 제11권1호
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• pp.19-29
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• 1998

참전복 사료에 적합한 단백질원을 조사하기 위하여 카제인, 어분, 육분, 우모분, 혈분, 대두박, 콘글루텐 밀, 면실박, 미역분을 첨가한 배합사료와 천연 먹이인 생미역과 건조 다시마를 선정하여 그 이용성을 비교하였다. 평균체중 0.11 g인 치패를 실험수조마다 70마리씩 수용하여 18주간 사육한 결과, 생존률은 카제인, 북양어분, 육분, 우모분, 미역분과 소맥분을 첨가한 실험구들이 서로 유의차없이(P>0.05) 타 실험구보다 높은 경향을 보였다. 실험구들의 최종평균체중은 0.27~0.74 g으로 북양어분을 첨가한 실험구가 0.74 g으로 가장 높았으며, 대두박 및 미역분말을 첨가한 실험구들이 북양어분 첨가구와 유의적인 차이없이(P>0.05) 양호한 결과를 보였다. 카제인 첨가구는 0.64 g으로 북양어분 첨가구와는 유의차를 보였으나(P<0.05). 다음으로 면실박과 소백분 첨가구가 0.55~0.57 g으로 양호한 성적을 보였으며, 육분 첨가구는 0.44 g, 천연 먹이인 생미역과 건조 다시마 공급구는 0.29~0.34 g, 우모분과 콘글루텐 밀 첨가구는 0.26~0.27 g으로 나타났으며, 혈분 첨가구가 0.19 g으로 가장 낮은 값을 보였다(P<0.05). 증체율과 각장 및 각폭도 평균체중 변화와 유사한 경향이었다. 실험 종료시 가식부의 수분과 단백질 함량은 각각 78.5~79.6% 및 13.2~14.2%로 사료의 단백질원에 거의 영향을 받지 않았다(P>0.05). 이상의 결과로부터 어분, 대두박, 면실박, 미역분말 또는 소백분은 참전복 사료의 훌륭한 단백질원이 될 것으로 기대되어 참전복 사료의 단가를 절감시키고, 사료의 질을 향상시키는데 큰 도움이 될 것으로 전망된다.