• BMB Reports
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• v.55 no.3
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• pp.113-124
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• 2022

Single-cell RNA sequencing (scRNA-seq) has greatly advanced our understanding of cellular heterogeneity by profiling individual cell transcriptomes. However, cell dissociation from the tissue structure causes a loss of spatial information, which hinders the identification of intercellular communication networks and global transcriptional patterns present in the tissue architecture. To overcome this limitation, novel transcriptomic platforms that preserve spatial information have been actively developed. Significant achievements in imaging technologies have enabled in situ targeted transcriptomic profiling in single cells at single-molecule resolution. In addition, technologies based on mRNA capture followed by sequencing have made possible profiling of the genome-wide transcriptome at the 55-100 ㎛ resolution. Unfortunately, neither imaging-based technology nor capture-based method elucidates a complete picture of the spatial transcriptome in a tissue. Therefore, addressing specific biological questions requires balancing experimental throughput and spatial resolution, mandating the efforts to develop computational algorithms that are pivotal to circumvent technology-specific limitations. In this review, we focus on the current state-of-the-art spatially resolved transcriptomic technologies, describe their applications in a variety of biological domains, and explore recent discoveries demonstrating their enormous potential in biomedical research. We further highlight novel integrative computational methodologies with other data modalities that provide a framework to derive biological insight into heterogeneous and complex tissue organization.

• 한국지구물리탐사학회:학술대회논문집
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• 1999.08a
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• pp.176-201
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• 1999

Principles, data acquisition, data processing of four frequently used borehole seismic methods, i.e., downhole seismic, vertical seismic profiling(VSP), crosshole seismic, and seismic tomography, are reviewed briefly. Field data examples are presented and their application to civil engineering area was also discussed.

• Mass Spectrometry Letters
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• v.5 no.4
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• pp.110-114
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• 2014

The method of direct mass spectrometry profiling is reliable and reproducible for the rapid identification of clinical isolates of bacteria and fungi. This is the first study evaluating the approach of MALDI-TOF mass spectrometry profiling for rapid identification of carbapenemase-resistant enterobacteriaceae (CRE). Proof of concept was achieved by the discrimination of CRE using MALDI Biotyper MS based on the protein. This profiling appears promising by the visual observation of consistent unique peaks, albeit low intensity, that could be picked up from the mean spectra (MSP) method. The Biotyper MSP creation and identification methods needed to be optimized to provide significantly improved differences in scores to allow for subspecies identification with and without carbapenemases. These spectra were subjected to visual peak picking and in all cases; there were pertinent differences in the presence or absence of potential biomarker peaks to differentiate isolates. We also evaluated this method for potential discrimination between different carbapenemases bacteria, utilizing the same strategy. Based on our data and pending further investigation in other CREs, MALDI-TOF MS has potential as a diagnostic tool for the rapid identification of even closely related carbapenemases but would require a paradigm shift in which Biotyper suppliers enable more flexible software control of mass spectral profiling methods.

• Health Policy and Management
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• v.20 no.4
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• pp.45-57
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• 2010

Background : If different cost efficiency indexes were informed to the same clinic depending on the inclusion or exclusion of pharmacy cost, it may impair the reliability of provider-profiling system. This study aimed to investigate whether the omission of pharmacy cost affects cost-efficiency rankings in medical clinics. Methods : Data for ambulatory care cost at 23,112 medical clinics were collected from the claims database, which was constructed after review by the Health Insurance Review and Assessment Service (HIRA) of Korea in April 2007. We calculated two types of cost efficiency indexes by inclusion or exclusion of pharmacy cost for a medical clinic. The agreement between the decile rankings of the two indexes was also assessed using the weighted kappa statistic of Landis and Koch. Results : When the cost efficiency index for total cost including pharmacy cost was compared with the index for total cost excluding it, the agreement between the two indexes was only 55%. The agreements between the two indexes were relatively low within specialties which have larger pharmacy volume of total cost and lower correlation between total cost with or without pharmacy cost included than the average level of all the specialties. Conclusion : These results suggest that the omission of pharmacy cost may result in contradictory outcomes that may be confusing to a medical institution and may impair the reliability of provider-profiling systems. It is very important to standardize profiling criteria for the reliability of provider profiling system.

• International Journal of Precision Engineering and Manufacturing
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• v.9 no.4
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• pp.79-82
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• 2008

A method is described for ideally reconstructing the profile from a surface profiling measurement containing a reasonable amount of null measurement data. The proposed method can conjecture lost information and rectify irregular data that result due to bad measuring environments, signal transmission noise, or instrument-induced errors, The method adopts the concept of computer graphics and consists of several processing steps. First, a search for valid data in the neighborhood of the null data is performed. The valid data are then grouped and their contours are extracted. By analyzing these contours, a bounding box can be obtained and the general distribution of the entire area encompassing the valid and null data is determined Finally, an ideal surface model is overlaid onto the measurement results based on the bounding box, generating a complete reconstruction of the calculations, A surface-profiling task on a liquid crystal display photo spacer is used to verify the proposed method. The results are compared to those obtained through the use of a scanning electron microscope to demonstrate the accuracy of the proposed method.

• Journal of Ginseng Research
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• v.48 no.2
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• pp.149-162
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• 2024

Ginseng, the roots of Panax species, is an important medicinal herb used as a tonic. As ginsenosides are key bioactive components of ginseng, holistic chemical profiling of them has provided many insights into understanding ginseng. Mass spectrometry has been a major methodology for profiling, which has been applied to realize numerous goals in ginseng research, such as the discrimination of different species, geographical origins, and ages, and the monitoring of processing and biotransformation. This review summarizes the various applications of ginsenoside profiling in ginseng research over the last three decades that have contributed to expanding our understanding of ginseng. However, we also note that most of the studies overlooked a crucial factor that influences the levels of ginsenosides: genetic variation. To highlight the effects of genetic variation on the chemical contents, we present our results of untargeted and targeted ginsenoside profiling of different genotypes cultivated under identical conditions, in addition to data regarding genome-level genetic diversity. Additionally, we analyze the other limitations of previous studies, such as imperfect variable control, deficient metadata, and lack of additional effort to validate causation. We conclude that the values of ginsenoside profiling studies can be enhanced by overcoming such limitations, as well as by integrating with other -omics techniques.

• Geophysics and Geophysical Exploration
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• v.7 no.1
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• pp.56-61
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• 2004

In order to image the distribution of permeability in granitic rocks, we carried out two-dimensional (2D) resistivity profiling, together with in-situ permeability tests, electrical logging of boreholes, and resistivity measurements of rock core samples in a laboratory. Based on the electrical logging and in-situ permeability data from boreholes, we obtained empirical equations which relate resistivity and permeability of the granitic rocks in the area studied. We then applied the empirical equation to a 2D resistivity section, to produce a 2D permeability section of the granitic rocks. In this paper, we present details of the field data and of the procedure for conversion from the resistivity section to a permeability section. The observed relationship between resistivity and permeability of the rocks is also discussed.

• ETRI Journal
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• v.42 no.2
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• pp.292-304
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• 2020

As side-channel analysis and machine learning algorithms share the same objective of classifying data, numerous studies have been proposed for adapting machine learning to side-channel analysis. However, a drawback of machine learning algorithms is that their performance depends on human engineering. Therefore, recent studies in the field focus on exploiting deep learning algorithms, which can extract features automatically from data. In this study, we survey recent advances in deep learning-based side-channel analysis. In particular, we outline how deep learning is applied to side-channel analysis, based on deep learning architectures and application methods. Furthermore, we describe its properties when using different architectures and application methods. Finally, we discuss our perspective on future research directions in this field.

• IEMEK Journal of Embedded Systems and Applications
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• v.17 no.1
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• pp.59-65
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• 2022

Virtual memory is used as the method to ensure the safety of the system through memory protection in the real-time system. TLB miss caused by using virtual memory makes the real-time system WCET more pessimistically. TLB lockdown can be applied as a method to improve this problem. However, processors with limited TLB lockdown entries, a selection criterion is needed to efficiently utilize the TLB lockdown entry. In this paper, the most frequently accessed virtual pages in the process are applied to the TLB lockdown by analyzing memory profiling. The results showed that micro data TLB miss stall cycle and main data TLB miss stall cycle of the processor decreased by at least 4.7% and up to 29.7%.

• The Journal of Korea Robotics Society
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• v.16 no.2
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• pp.94-102
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• 2021

This study addresses a method for 3D reconstruction using acoustic data with heterogeneous sonar devices: Forward-Looking Multibeam Sonar (FLMS) and Profiling Sonar (PS). The challenges in sonar image processing are perceptual ambiguity, the loss of elevation information, and low signal to noise ratio, which are caused by the ranging and intensity-based image generation mechanism of sonars. The conventional approaches utilize additional constraints such as Lambertian reflection and redundant data at various positions, but they are vulnerable to environmental conditions. Our approach is to use two sonars that have a complementary data type. Typically, the sonars provide reliable information in the horizontal but, the loss of elevation information degrades the quality of data in the vertical. To overcome the characteristic of sonar devices, we adopt the crossed installation in such a way that the PS is laid down on its side and mounted on the top of FLMS. From the installation, FLMS scans horizontal information and PS obtains a vertical profile of the front area of AUV. For the fusion of the two sonar data, we propose the probabilistic approach. A likelihood map using geometric constraints between two sonar devices is built and a monte-carlo experiment using a derived model is conducted to extract 3D points. To verify the proposed method, we conducted a simulation and field test. As a result, a consistent seabed map was obtained. This method can be utilized for 3D seabed mapping with an AUV.