• Title/Summary/Keyword: Productivity analysis

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Culture Conditions of E. coli Harboring Human O-Linked N-Acetyl-${\beta}$-Glucosaminidase Gene and Enzymatic Properties (사람의 O-linked-N-acetyl-${\beta}$-D-glucosaminidase 유전자를 함유한 대장균의 배양조건과 효소학적 특성)

  • 강대욱;조용권;서현효
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.147-153
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    • 2004
  • Protein modification by N-acetyl-${\beta}$-D-glucosamine (O-G1cNAc) on the hydroxyl groups of Ser or Thr ubiq-uitously occurs in eukaryotic cells and is involved in many cellular phenomena. The level of O-G1cNAc-mod-ified protein is regulated by OGT and O-GlcNAcase enzymes. We have tried to produce recombinant O-GlcNAcase in E. coli as an effort to establish in vitro screening system for modulators of O-GlcNAcase. The culture conditions for improvement of O-GlcNAcase productivity, were as follows: induction temperature, $30^{\circ}C$; the concentration of L-arabinose, 0.02% and induction time, 5 hr. Under these culture conditions, E. coli cells containing O-GlcNAcase gene had no enzyme activity until up to 3 hr culture. However, O-GlcNAcase activity dramatically increased from 3 to 5 hr culture. It almost maintained the same level after 5 hr culture. Western blot analysis verified the amount of expressed O-GlcNAcase increased with culture time, being con-sistent with activity data. The optimal reaction condition determined in this study was as follows: protein quan-tity, $5{\mu}g$; reaction time, 30 min; reaction temperature, $45^{\circ}C$; substrate concentration, 2 mM; reaction pH, 6.5. Methanol had little effect on O-GlcNAcase activity and 90% of activity were retained at 10%. Only 15% resid-ual activity were detected at 5% of chloroform.

Establishment of a quality index of surfactin production during cheonggukjang fermentation (청국장 발효 중 surfactin 생산량 품질지표 설정)

  • Chang-Geun Ji;Jeong-Hwa Hong;Sang-Hyun Lee
    • Food Science and Preservation
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    • v.30 no.2
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    • pp.311-320
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    • 2023
  • Surfactin, one of the most powerful biosurfactants, can be widely applied in agriculture, food, and pharmaceutics. The purpose of the present study was to establish suitable indicators for a rapid detection method that can confirm the surfactin productivity of cheonggukjang. In the present study, changes in the total number of bacteria were quantified according to the fermentation time of chenggukjang. Furthermore, physicochemical factors, such as pH, color value, surface tension, refractive index, absorbance, and protein contents, were assessed in chenggukjang viscous substances, and a correlation analysis between the physicochemical factors and surfactin contents was performed. We found that the protein contents gradually increased up to 48 h (4.21±0.11 mg/mL) without a change in the surface tension. Furthermore, the refractive index, absorbance at 280 nm, and color value were significantly increased as the fermentation time increased; however, there were no statistically significant differences after 36 h of fermentation. Interestingly, this result exhibited a tendency similar to the surfactin content according to fermentation time. These findings suggest that surfactin can be used as a suitable quality indicator and may provide an experimental basis for the fermented food industry.

A Study about Impact of Mindfulness on Perceived Factors of Information Technology Acceptance (마음챙김이 정보기술 수용의 인지적 요인에 미치는 영향 연구)

  • Hyun Mo Kim;Ying Ying Pang;Joo Seok Park
    • Information Systems Review
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    • v.21 no.1
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    • pp.1-22
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    • 2019
  • Mindfulness is the process of actively noticing new things. Today, companies have introduced and run mindfulness programs because the mindfulness has possible applications of productivity and innovation in corporation. However, role of mindfulness has not been clearly investigated in behavior research of Information System. The purpose of this study is to confirm the effects of mindfulness on technology acceptance process. Based on UTAUT Model, we examined how mindfulness in technology acceptance process moderate antecedent factors of acceptance intentions and use behavior. For empirical research, we conducted a survey on acceptance of smart watch of internet of things for employees of companies applying the mindfulness programs. then, we analyzed survey sample in empirical methodologies. Based on the empirical analysis, cognizance of alternative technologies in mindfulness factors increased the impact of performance expectancy on acceptance intention. Novelty seeking in mindfulness factors increased the impact of effort expectancy on acceptance intention. Awareness of local context in mindfulness factors decreased the impact of social influence on acceptance intention. engagement with technology in mindfulness factors increased the impact of facilitating conditions on use behavior. This study suggests academic implications and practical implications based on the results of the research. The implications will help to support and extend the theory of technology acceptance model while providing practical insights for IT acceptance by suggesting ways to utilize mindfulness in corporation.

Current Status and Perspective of Smart Vegetable Seedling Production Technology in the Republic of Korea (국내 스마트 채소 육묘 기술 개발 현황 및 전망)

  • Dong Hyeon Kang;So Young Lee;Hey Kyung Kim;Sewoong An
    • Journal of Practical Agriculture & Fisheries Research
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    • v.26 no.1
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    • pp.22-29
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    • 2024
  • In this study, we summarized the definition of smart vegetable seedling production technology, analysis of smart seedling production system, a hardware and software configuration model for smart seedling production system, research and development trends in smart seedling production system, and proposed future research and development plans for smart seedling production technology. Smart vegetable seedling production is a data-based seedling production, management, and distribution system that utilizes 4th Industrial Revolution technology to improve seedling productivity and quality. The production of vegetable seedlings using smart seedling production technology can be efficiently managed by collecting, analyzing, and managing information on seedlings, environment, and tasks at each stage of production by linking with the smart seedling integrated management system. However, there is still a lack of standardization of seedling standards and quality for each vegetable crop to establish smart seeding production technology, as well as development of smart seedling production element technology, which requires national wide R&D support.

Improvement of the Thinning System by Exploring the Stand Density Management Criteria for Chamaecyparis obtusa in South Korea (편백림의 임분밀도 관리 기준 탐색을 통한 시업체계 개선)

  • Su Young Jung;Kwang Soo Lee;Hyun Soo Kim;Joon Hyung Park
    • Journal of Korean Society of Forest Science
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    • v.113 no.1
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    • pp.131-142
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    • 2024
  • The purpose of this study was to estimate the optimal stand density criteria for each growth stage of Chamaecyparis obtusa (Siebold & Zucc.) Endl. to achieve the timber production goal for cypress forests and develop an optimal silvicultural system for forest thinning. A relative yield index (Ry) value of 0.75, presented as a stand density management criterion index, was estimated by analyzing the relationship characteristics between the composition ratio and stand density of slender trees from 216 sample plots of the recruited cypress forests. The analysis of the feasibility of achieving each production target in the existing silvicultural system for C. obtusa revealed that the growth rate according to the parameters of forest land productivity, such as site index and thinning intensity according to the increase in age, was not properly reflected. In the thinning system for each timber production target analyzed in this study, 353 high-quality large hardwoods from 498.1 m3/ha, 703 high-quality medium hardwoods from 376.2 m3/ha, and 1,758 small-diameter hardwoods from 249.5 m3/ha could be harvested. Although the silvicultural system prepared on the basis of the results of this study cannot be uniformly applied according to various management goals, this study is meaningful in that it presents empirical reference standards based on the stand density management diagram that reflects the growth characteristics of cypress forests in South Korea.

Study on Customer Satisfaction Performance Evaluation through e-SCM-based OMS Implementation (e-SCM 기반 OMS 구현을 통한 고객 만족 성과평가에 관한 연구)

  • Hyungdo Zun;ChiGon Kim;KyungBae Yoon
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.3
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    • pp.891-899
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    • 2024
  • The Fourth Industrial Revolution is centered on a personalized demand fulfillment economy and is all about transformation and flexible processing that can deliver what customers want in real time across space and time. This paper implements the construction and operation of a packaging platform that can instantly procure the required packaging products based on real-time orders and evaluates its performance. The components of customer satisfaction are flexible and dependent on the situation which requires efficient management of enterprise operational processes based on an e-SCM platform. An OMS optimized for these conditions plays an important role in maximizing and differentiating the efficiency of a company's operations and improving its cost advantage. OMS is a system of mass customization that provides efficient MOT(Moment of Truth) logistics services to meet the eco-friendly issues of many individual customers and achieve optimized logistics operation goals to enhance repurchase intentions and sustainable business. OMS precisely analyzes the collected data to support information and decision-making related to efficiency, productivity, cost and provide accurate reports. It uses data visualization tools to express data visually and suggests directions for improvement of the operational process through statistics and prediction analysis.

The Effect of Rice Straw and Tall Fescue Hay for Holstein Heifers on the Productivity and Economics of Lactating Cow (홀스타인 육성기에 볏짚 및 톨페스큐 건초 급여가 착유우의 생산성 및 경제성에 미치는 영향)

  • Jin Su Ryu;Shin Ja Lee;Hyun Jin Kim;Sung Sill Lee
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.44 no.2
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    • pp.83-91
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    • 2024
  • We compared the reproductive performance, non-productive performance, and milk composition performance of two groups of lactating cows that were either rice straw or tall fescue. We also compared and analyzed these parameters after the first parturition during the breeding period. The calving interval, artificial insemination, and duration from calving to conception were longer in the rice straw group than in the tall fescue group. The 305-day and peak milk yields were significantly higher in the tall fescue group than in the rice straw group (p<0.01). Milk fat content was significantly higher in the tall fescue group than in the rice straw group (p<0.001), but milk protein content was the same between the feeding groups. The difference in raw milk sales income between rice straw and tall fescue groups tended to increase as the number of lactations increased, but there was no effect of reduced feed costs. Analysis of the milk production and raw milk sales income of the surveyed farms revealed that the average milk production per head was significantly (p<0.05) higher in the tall fescue group than in the rice straw group, and raw milk sales income showed a similar tendency. Overall, these results provide important details to be considered when selecting feed as a way to reduce milk production and heifer raising costs. However, there remains a need for future follow-up studies exploring the relationship between feed choice and the management of heifers.

Overexpression of rice NAC transcription factor OsNAC58 on increased resistance to bacterial leaf blight (전사인자 OsNAC58 과발현을 통한 벼 흰잎마름병 저항성 증진 벼)

  • Park, Sang Ryeol;Kim, Hye Seon;Lee, Kyong Sil;Hwang, Duk-Ju;Bae, Shin-Chul;Ahn, Il-Pyung;Lee, Seo Hyun;Kim, Sun Tae
    • Journal of Plant Biotechnology
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    • v.44 no.2
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    • pp.149-155
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    • 2017
  • Bacterial blight in rice caused by Xanthomonas oryzae pv. oryzae (Xoo) greatly reduces the growth and productivity of this important food crop. Therefore, we sought to increase the resistance of rice to bacterial blight by using a NAC (NAM, ATAF, and CUC) transcription factor, one of the plant-specific transcription factors that is known to be involved in biotic/abiotic stress resistance. By isolating the OsNAC58 gene from rice and analyzing its biological functions related to Xoo resistance, phylogenetic analysis showed that OsNAC58 belongs to group III. To investigate the biological relationship between bacterial leaf blight (BLB) and OsNAC58 in rice, we constructed a vector for overexpression in rice and generated transgenic rice. The expression analysis resulting from use of RT-PCR showed that OsNAC58-overexpressed transgenic rice exhibited higher levels of OsNAC58 expression than wild types. Further, subcellular localization analysis using rice protoplasts showed that the 35S/OsNAC58-SmGFP fusion protein was localized in the nuclei. Thirteen OsNAC58-overexpressed transgenic rice lines, with high expression levels of OsNAC58, showed more resistant to Xoo than did the wild types. Together, these results suggest that the OsNAC58 gene of rice regulates the rice disease resistance mechanism in the nucleus upon invasion of the rice bacterial blight pathogen Xoo.

A Study on the Effects of Meterological Factors on the Distribution of Agricultural Products: Focused on the Distribution of Chinese Cabbages (기상요인이 농산물 유통에 미치는 영향에 관한 연구: 배추 유통 사례를 중심으로)

  • Lee, Hyunjoung;Hong, Jinhwan
    • Journal of Distribution Research
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    • v.17 no.5
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    • pp.59-83
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    • 2012
  • Agriculture is a primary industry that influenced by the weather or meterological factors more than other industry. Global warming and worldwide climate changes, and unusual weather phenomena are fatal in agricultural industry and human life. Therefore, many previous studies have been made to find the relationship between weather and the productivity of agriculture. Meterological factors also influence on the distribution of agricultural product. For example, price of agricultural product is determined in the market, and also influenced by the weather of the market. However, there is only a few study was made to find this link. The objective of this study is to investigate the effects of meterological factors on the distribution of agricultural products, focusing on the distribution of chinese cabbages. Chinese cabbage is a main ingredient of Kimchi, and basic essential vegetable in Korean dinner table. However, the production of chinese cabbages is influenced by weather and very fluctuating so that the variation of its price is so unstable. Therefore, both consumers and farmers do not feel comfortable at the unstable price of chinese cabbages. In this study, we analyze the real transaction data of chinese cabbage in wholesale markets and meterological factors depending on the variety and geography. We collect and analyze data of meterological factors such as temperatures, humidity, cloudiness, rainfall, snowfall, wind speed, insolation, sunshine duration in producing and consuming region of chinese cabbages. The result of this study shows that the meterological factors such as temperature and humidity significantly influence on the volume and price of chinese cabbage transaction in wholesale market. Especially, the weather of consuming region has greater correlation effects on transaction than that of producing region in all types of chinese cabbages. Among the whole agricultural lifecycle of chinese cabbages, 'seeding - harvest - shipment - wholesale', meterological factors such as temperature and rainfall in shipment and wholesale period are significantly correlated with transaction volume and price of crops. Based on the result of correlation analysis, we make a regression analysis to verify the meterological factors' effects on the volume and price of chines cabbage transaction in wholesale market. The results of stepwise regression analysis are shown in

    and
    . The type of chinese cabbages are categorized by 5 types, i.e. alpine, gimjang for winter, spring, summer, and winter crop, and all of the regression models are shown significant relationship. In addition, meterological factors in shipment and wholesale period are entered more in regression model than those in seeding and harvest period. This result implies that weather in consuming region is also important in the distribution of chinese cabbages. Based on the result of this study, we find several implications and recommendations for policy makers of agricultural product distribution. The goal of agricultural product distribution policy is to insure proper price and production cost for farmers and provide proper price and quality, and stable supply for consumers. Therefore, coping with the uncertainty of weather is very essential to make a fruitful effect of the policy. In reality, very big part of consumer price of chinese cabbage is made up of the margin of intermediaries, because they take the risk. In addition, policy makers make efforts for farmers to utilize AWIS (Agricultural Weather Information System). In order to do that, it should integrate the relevant information including distribution and marketing as well as production. Offering a consulting service to farmers about weather management is also expected to be a good option in agriculture and weather industry. Reflecting on the result of this study, the distribution authorities can offer the guideline for the timing and volume of harvest, and it is expected to contribute to the stable equilibrium of supply and demand of agricultural products.

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  • Occupational Stress of Hospital Workers (병원종사자의 직업성 스트레스에 관한 연구 - 서울시내 500병상 이상 병원종사자를 중심으로 -)

    • Lee, Woo-Cheon
      • Korea Journal of Hospital Management
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      • v.3 no.1
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      • pp.1-33
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      • 1998
    • The purpose of this thesis is to study theoretical access to the methods that have been used for the research of occupational stress, thereby providing management methods of occupational stress of hospital workers. With a stress model of hospital workers set up from the viewpoint of organization management, 929 sets of questionnaires were collected from intern doctors, resident doctors, nurses, nursing aides, pharmacists, medical technicians, workers in patient affairs(reception and medical insurance workers), administrators and clerks from the 8 hospitals in Seoul with more than 500 beds. Upon variance analysis, correlation analysis and regression analysis of the collected questionnaires, this work examined how differences in stress caused by specific occupations and formulated a method of stress management for the hospital workers. The results are as follows. 1) If some duties of the nurses suffering from role-overloaded stress are transferred to the nursing aides dissatisfied with insufficient role, the two grunting groups can be satisfied at the same time. It is also necessary to transfer some jobs of the overloaded workers in patient affairs to the administrators, or the other way around. To reduce stress of conflict and ambiguity of role caused by the obscure division of roles between the workers, the role of each occupation should be delineated and the clear division of roles should be translated into action strictly according to that delineated. 2) Stress of inefficiency of organization from which the student doctors suffer can be relieved by management of participation. If they have access to the process of decision-making in general hospital affairs and consequently their understanding and the autonomy of job performance are promoted, such stress will be reduced. 3) To cope with stress of career development from which nurses, medical technicians, administrators, workers in patient affairs suffer, it is necessary to establish whether they have a chance to revive their careers, whether there are any ways of remotivation for less contributive workers, and whether they encourage each other to develope their careers. If they are given a chance to develope their careers, such stress will be relieved. 4) Pharmacists, suffering from stresses of living and personal relations, have strong cohesive power among themselves and organize a well-integrated team; thereby reducing the stress of personal relations and increasing productivity. 5) For administrators and student doctors confined to lesser social supports and for nurses and workers in patient affairs whose recognition of stress and job satisfaction are affected by social supports, emotional and informational supports for job performance help alleviate an individual's mental, and physical stress. 6) In addition to the above-mentioned stress-management methods, if an organizational coping strategy is provided according to the types of stress from the general viewpoint of the whole group of hospital workers, it would be of great help to managing stress. For example, the redesign of jobs, the management of objective, the improvement of working environment, the formation of an autonomous working group and various working plans can be set up for those who suffer from stress related to inappropriate role, while career counseling and development of career process can be provided for those dissatisfied with career development. Participation in the process of decision-making and the restructuring of the organization are needed for those who suffer from stress of malfunctioning organization, whereas creation of a supportive organizational atmosphere is desired for those who feel stressed due to personal relations. As well, such organizational coping strategies. as the increase of welfare facilities, seminars and educational programs and provision of health-promotion facilities can be provided.

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