• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.52-52
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• 2018

Development of herbicide resistant transgenic legume plants through Agrobacterium-mediated transformation has been worked in many previous studied. Plant regeneration after infection is the important step to obtain successful transgenic plants. Many attempts try to find the optimum media condition for plant regeneration after infection. However, the transformation efficiency of legume plants is still low. In this study, regeneration of some Korean legume species including two soybean cultivars (Dawon and Pungsan) and pea have been done with organogenesis which is used various kind of explants such as cotyledonary-nodes in soybean and bud-containing tissue in pea. We developed the optimum media condition for plant regeneration regulators under Agrobacterium-mediated transformation using different kind and various concentration of plant growth. As the results, B5 medium containing 2 mg/L of 6-benzylaminopurine was selected in this study for the optimum plant regeneration media. The segments were inoculated with Agrobacterium suspension harbored an IG2 vector containing bar gene which confers resistance to phosphinotricin (PPT) in 3, 5 and 7 days. The transformation efficiency was achieved in Dawon 3.03 % and pea 1.46 % with co-cultivation period of 7 days which is showed a high number of GUS positive expression period.

• Applied Biological Chemistry
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• 제27권
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• pp.3-17
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• 1984

Anaerobic digestion has recently attracted all over the world and Korea also shows no exception. The major benefits of anaerobic digestion are energy production, water pollution control, pathogen reduction and effective manure production. In Korea it was recognized in late sixties that there was a positive need to find alternative energy for farmers household. The main traditional energy sources in rural area were crop residues and forestry products. Therefore Office of Rural Development through its Rural Guidance Bureau disseminated about 29,000 household biogas units from 1969 to 1975 to provide cooking fuel for farmers household and to improve the mode of farmers living standards. The units were welcomed by farmers at that time. Now, however, most of them are not using due to a number of reasons associated with cold winter and some techno-economical problems (in those day, fossil fuel was quite expensive to compare with other prices and since then farmers income was quickly increased). The author studied on bag type household biogas plant to solve some technical problems of existing household biogas plants, but this also has little appeal for the farmers. From 1977 author studied on village scale biogas plant with two pilot plants. From the viewpoint of energy production, COD removal, kill rate of pathogen and fertilizer value, the results obtained from the experiments were quite promising, but the construction cost of the village scale biogas plant was too high for the farmers in Korea. To find most suitable biogas plant for farmers in Korea through the simplifying the biogas digester, the author developed batch-load biogas plant. By feeding coarse crop residues and manures, total solids concentrations of the batch-load biogas plant are about 28 percent which is much higher than continous digester of 5-8 percent. The batch-load biogas plant was welcomed by many farmers in Korea when it was reported on TV and newspapers. The plant was disseminated 154 units in 1982, 766 units in 1983 and 812 units in 1984 as a promissing project. Besides these biogas plant experiments, studies were also conducted 1) to determine gas production rate with agricultural wastes, 2) to evaluate the effect of loading rate, dilution, retention time on biogas production, 3) to project the amount of potencial energy from agricultural wastes.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.88-88
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• 2022

In this study, we investigated the effect of Solanum nigrum aerial parts (SNAP) on macrophage activation and macrophage autophagy in RAW264.7 cells. SNAP increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. TLR4 inhibition blocked SNAP-mediated production of immunostimulatory factors. In addition, the JNK inhibition reduced the SNAP-mediated production of immunostimulatory factors, and the SNAP-mediated JNK activation was blocked by the TLR4 inhibition. SNAP activated macrophage autophagy. TLR4 inhibition blocked SNAP-mediated macrophage autophagy and inhibition of p38 and JNK attenuated SNAP-mediated macrophage autophagy. These findings indicate that SNAP may induce TLR4/JNK-mediated macrophage activation and TLR4/p38 and JNK-mediated macrophage autophagy.

• 원예과학기술지
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• 제35권4호
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• pp.439-445
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• 2017

Transplant production in a plant factory with artificial lighting provides several benefits; (1) rapid and uniform transplant production, (2) high production rate per unit area, and (3) production of disease free transplants production. To improve the growth of runner plants when strawberry transplants are produced in a plant factory, we conducted two experiments to investigate (1) the effect of different light intensity for stock and runner plants on the growth of runner plants, and (2) the effect of different container volume for runner plants on their growth. When the stock and runner plants were grown under nine different light conditions composed of three different light intensities (100, 200, and $400{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPF) for each stock and runner plants, increasing the light intensity for stock plants promoted the growth of runner plants, however, the growth of runner plants was not enhanced by increasing the light intensity for runner plants under same light intensity condition for stock plants. We also cultivated runner plants using plug trays with four different container volumes (21, 34, 73, and 150 mL) for 20 days after placing the stock plants, and found that using plug trays with lager container volume did not enhance the growth of runner plants. These results indicate that providing optimal condition for stock plants, rather than the runner plants, is more important for increasing the growth of the runner plants and that the efficiency of strawberry transplant production in a plant factory can be improved by decreasing light intensity or container volume for runner plants.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.36-36
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• 2018

Soybean is the important crop in Asian countries as protein source, oil production and animal feed. Improving soybean using genetic transformation is the principal tool in nowadays. Developing herbicide resistant transgenic soybean plants through Agrobacterium-mediated transformation has been worked in many previous studied. However, the transformation efficiency is still low. Many attempts try to find the optimum media condition for plant regeneration after infection. After transformation, the plant regeneration is very important condition to promote growth of transgenic plant. In this study, we optimized a regeneration condition for two Korean soybean cultivar, Dawonkong and Pungsannamulkong using cotyledon, cotyledonary nodes and hypocotyl as explant. The results showed that shoot regeneration of cotyledonary nodes on B5 medium containing 2 mg/L 6-benzylaminopurine showed the highest percentage of regeneration in Dawonkong (75.8%) while Pungsannamulkong presented high number of shoots 2.12 shoots per explant. For transformation condition, co-cultivation in 7 days showed a high number of GUS positive expression. Most of explants can survived under media including 5 mg/L of glufocinate which refers phosphinotricin for 2-week selection. Washing with 400 mg/L of cefotaxime in several times and selection in plant regeneration media with 400 mg/L of cefotaxime can prevent bacteria growth, effectively.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.162-162
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• 2003

• The International Journal of Costume Culture
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• 제13권1호
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• pp.23-25
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• 2010

Archaeological evidence shows that cotton fibres of the genus Gossypium were first used for textile production at Mehrgarh, Pakistan in the Neolithic period ($6^{th}$ millenium BC). Eventually the cotton plant and textiles found their way to Egypt and through there, to Greece. However, the chronological origins of the cultivation and/or use of cotton fibres in textile production in ancient Greece are ambiguous. The main sources of information are ancient written texts and excavated textile finds. Both indicate that the introduction of cotton in Greece can be placed in the mid-first millenium BC. However, it is not clear whether the cultivation of the plant and consequently the production of cotton textiles was established in that period or whether another cotton species was produced and used locally in Greece or whether isolated examples of cotton textiles had been imported in Greece before the cotton plant. A more comprehensive analytical study of surviving textile finds coupled with archaeological research for plant production and cultivation would provide the answer to these questions.

• Agricultural and Biosystems Engineering
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• 제5권2호
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• pp.55-59
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• 2004

This study aimed to develop a seeder for sowing large seeds such as those of fruit vegetables used as rootstocks in grafting. It investigated how the nozzle diameter and the nozzle's vacuum pressure affected the seeding performance. This study found that a nozzle diameter of 1.5 mm was capable of sowing most of the seed sizes used in this study. The highest seeding rates for 'Chambak', 'Tuktozwa', and 'Hukjong' were $97.6\%$, $98.8\%$, and $97.6\%$, respectively. Lineup status after sowing was good. The working performance was 75.6 sheets/hr. An average seeding rate of 1 grain was $97.8\%$.

• 한국자원식물학회지
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• 제22권6호
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• pp.518-521
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• 2009

High productivity of ginger (Zingiber officinale Roscoe) was obtained from the rhizome produced by shoot-tip culture with Korean native variety, Seosanjong. Seed rhizomes induced by shoot-tip culture were successfully established in the field. The rhizomes induced by both plant or rhizome were higher in emergence rate and faster in days to emergence than those of home seed production. The seed rhizome production induced by shoot-tip culture was two times heavier than that of home seed production. These results suggest that shoot-tip culture might be one of mass propagation methods in seed rhizome of ginger plant.

• Journal of Plant Biology
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• 제34권4호
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• pp.325-330
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• 1991

The ability of several auxin analogs to induce ethylene production was tested in the corn coleoptile. The synthetic auxins 1-naphthaleneacetic acid (1-NAA) and 2, 4-dichlorophenoxyacetic acid (2, 4-D) had strong stimulatory effects on ethylene induction surpassing that of IAA. Both 2-naphthalaneacetic acid (2-NAA) and 2, 6-dichlorophenoxy acetic acid (2, 6-D), structural analogs of these auxins, respectively, were found to be inactive. Treatment with NPA, a strong inhibitor of polar auxin transport, led to drastic increase in IAA-induced ethylene production while it has bo effect on ethylene production induced by 1-NAA. A positive correlative existed between intracellular auxin level and ethylene production.