• Journal of Life Science
• /
• v.18 no.1
• /
• pp.52-57
• /
• 2008

Xylan is a major hemicellulose component of the cell walls of monocots and hardwood, representing up to 30% of the dry weight of these plants. To efficiently hydrolyze xylan, the endoxylanase gene from Bacillus sp. was expressed in B. subtilis DB431 by introducing the plasmid pJHKJ4. The total activity of the recombinant endoxylanase reached about 857 unit/ml by batch fermentation of B. subtilis DB431/pJHKJ4 in LB maltose medium. The majority (>92%) of endoxylanase was efficiently secreted into the culture medium. The recombinant endoxylanase hydrolyzed more the birchwood xylan efficiently than the other xylans. When 4 % concentration of xylan was used, the highest production of xylooligosaccharide was observed, and xylobiose and xylotriose were the major products. Optimal amount of enzyme and reaction time for producing xylooligosaccharide were found to be 10 unit and 1 hr, respectively. In addition, the temperature of $40^{\circ}C{\sim}50^{\circ}C$ gave the highest production of xylooligosaccharide. Consequently, the optimized conditions for the production of xylooligosaccharide through the hydrolysis of xylan were determined as follows: 10 unit endoxylanase, $50^{\circ}C$, 4% birchwood xylan, 1 hr reaction.

• Applied Biological Chemistry
• /
• v.43 no.1
• /
• pp.12-17
• /
• 2000

For the enzymatic production of $2-O-{\alpha}-D-glucopyranosyl-L-ascorbic$ acid (AA-2G) from ascorbic acid, rice seed was used as the source of ${\alpha}-glucosidase$ having transglucosylation activity. Among six rice varieties, cultivated in Korea, ${\alpha}-glucosidase$ activity of Oryza savita L. cv. Ilpumbyeo was the highest with 125.03 unit/ml and it had maximum specific activity with 8.52 unit/mg protein when rice seeds were grown for 3 days after germination. For the production of AA-2G using crude extract of O. savita L. cv. Ilpumbyeo, maltose was most effective glucose donor. The optimum concentration of maltose and ascorbic acid were 125 mM and 175 mM, respectively. The optimum concentration of ${\alpha}-glucosidase$ was 100 unit. The most effective buffer was 100 mM sodium citrate. The optimum pH and temperature were 5.0 and $60^{\circ}C$, respectively. Under the optimum condition, $108.43\;{\mu}M/unit$ of AA-2G was produced from ascorbic acid after 35 minutes of reaction, which corresponds to 6.2% of conversion ratio based on the amount of ascorbic acid used.

• Genomics & Informatics
• /
• v.21 no.4
• /
• pp.47.1-47.12
• /
• 2023

Silver barb (Barbonymus gonionotus) is among the most economically important freshwater fish species in Thailand. It ranks fourth in economic value and third in production weight for fisheries and culture in Thailand. An XX/XY sex-determination system based on gynogenesis was previously reported for this fish. In this study, the molecular basis underlying the sex-determination system was further investigated. Genome-wide single-nucleotide polymorphism data were generated for 32 captive-bred silver barb individuals, previously scored by phenotypic sex, to identify sex-linked regions associated with sex determination. Sixty-three male-linked loci, indicating putative XY chromosomes, were identified. Male-specific loci were not observed, which indicates that the putative Y chromosome is young and the sex determination region is cryptic. A homology search revealed that most male-linked loci were homologous to the Mariner/Tc1 and Gypsy transposable elements and are probably the remnants of an initial accumulation of repeats on the Y chromosome from the early stages of sex chromosome differentiation. This research provides convincing insights into the mechanism of sex determination and reveals the potential sex determination regions in silver barb. The study provides the basic data necessary for increasing the commercial value of silver barbs through genetic improvements.

• Journal of the Korean Society of Costume
• /
• v.55 no.2 s.92
• /
• pp.116-128
• /
• 2005

The aim of this study is to understand problems with both fit and sizing system of gloves by analyzing the glove production industries with an emphasis on the sizing system, production measurement system, and general marking situation. Also, to suggest the basic raw materials for improving sizing system for gloves, actual glove control sizes were compared to the anthropometric data of the previous study Fifteen domestic production companies were participated in this study-Domestic glove production companies established their own sizing system by copying inter-national brand's glove sizing system or by their experience. The Korean Standard of gloves and the 1997 Korean Standard Anthropometrics Measurement for producing glove patterns are not considered because of its discordance with the reality of the required measurements. Domestic glove production companies used different size designation and labeling system. Size measurement unit also showed difference among the glove production companies. Some companies used 'cm', some used 'mm', some used 'inch' for the measurement unit. In general, companies produced 5 to 4 sizes in one design of glove and the production was the highest in M and L size. In 9 out of 15 companies preferred control size as hand length and hand circumference. For reference size, most of the companies preferred finger circumference, finger length, palm length, hand breadth, crotch height, and hand thickness. Actual glove sizes were compared to the anthropometric data of the previous study. The results indicated that most of the measurements of actual glove sizes were significantly larger than the anthropometric data.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.15 no.3
• /
• pp.124-132
• /
• 2010

In spite of the global importance of primary production of phytoplankton, some primary production data in Korean coastal waters still need to be better processed. The daily rates of water column primary production is generally estimated by integrating the primary production per unit volume over time and depth, but efforts for time integration algorithm have been conducted insufficiently. In this study a mathematical equation evaluating daily primary production integrated over time of a day is proposed and the effectiveness of the model is tested on Saemangeum Lake. The daily primary productions computed with the proposed equation were nearly the same with the results numerically integrated by substituting solar irradiance data. It was suggested that better estimation of primary production would be obtained by using monthly or weekly means of solar irradiance rather than more variable daily data. Because of the vertically heterogenous distribution of phytoplankton, it's hard to integrate the equation over depth to give the daily rates of primary production per unit area of water surface. However, the problem would be solved if, after the vertical distribution of phytoplankton was classified into several patterns and reduced to mathematical formula, every composite function of time integrated equation and chlorophyll distribution equation was integrated successfully.

• The Journal of Fisheries Business Administration
• /
• v.46 no.2
• /
• pp.59-74
• /
• 2015

This study aims to estimate optimal harvesting production, fishing efforts, and stock levels of yellow croaker caught by the offshore Stow Net and the offshore Gill Net fisheries using the current value Hamiltonian method and the surplus production model. As analyzing processes, firstly, this study uses the Gavaris general linear model to estimate standardized fishing efforts of yellow croaker caught by the above multiple fisheries. Secondly, this study applies the Clarke Yoshimoto Pooley(CY&P) model among the various exponential growth models to estimate intrinsic growth rate(r), environmental carrying capacity(K), and catchability coefficient(q) of yellow croaker which inhabits in offshore area of Korea. Thirdly, the study determines optimal harvesting production, fishing efforts, and stock levels of yellow croaker using the current value Hamiltonian method which is including average landing price of yellow croaker, average unit cost of fishing efforts, and social discount rate based on standard of the Korean Development Institute. Finally, this study tries sensitivity analysis to understand changes in optimal harvesting production, fishing efforts, and stock levels of yellow croaker caused by changes in economic and biological parameters. As results drawn by the current value Hamiltonian model, the optimal harvesting production, fishing efforts, and stock levels of yellow croaker caught by the multiple fisheries were estimated as 19,173 ton, 101,644 horse power, and 146,144 ton respectively. In addition, as results of sensitivity analysis, firstly, if the social discount rate and the average landing price of yellow croaker continuously increase, the optimal harvesting production of yellow croaker increases at decreasing rate and then finally slightly decreases due to decreases in stock levels of yellow croaker. Secondly, if the average unit cost of fishing efforts continuously increases, the optimal fishing efforts of the multiple fisheries decreases, but the optimal stock level of yellow croaker increases. The optimal harvest starts climbing and then continuously decreases due to increases in the average unit cost. Thirdly, when the intrinsic growth rate of yellow croaker increases, the optimal harvest, fishing efforts, and stock level all continuously increase. In conclusion, this study suggests that the optimal harvesting production and fishing efforts were much less than actual harvesting production(35,279 ton) and estimated standardized fishing efforts(175,512 horse power) in 2013. This result implies that yellow croaker has been overfished due to excessive fishing efforts. Efficient management and conservative policy on stock of yellow croaker need to be urgently implemented.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.12
• /
• pp.1661-1666
• /
• 2009

The objective of this study was to determine the effect of Se-enriched bean sprout, Se-enriched yeast and sodium selenite on productivity, egg quality and egg Se concentrations in laying hens. Using a Completely Randomized Design, 144 Rohman laying hens at 71 weeks of age were divided into four groups. Each group consisted of four replicates and each replicate contained nine hens. The dietary treatments were T1: control diet, T2: control diet plus 0.3 mg Se/kg from sodium selenite, T3: control diet plus 0.3 mg Se/kg from Se-enriched yeast, T4: control diet plus 0.3 mg Se/kg from Se-enriched bean sprout. The results showed that there was no significant difference (p>0.05) in feed intake, egg production and egg quality among treatments. Selenium supplementation from Seenriched yeast and Se-enriched bean sprout markedly increased (p<0.05) egg Se concentration as compared to the control and sodium selenite groups. The results indicated that Se-enriched bean sprout could be used as an alternative Se source in diets of laying hens.

• KIEAE Journal
• /
• v.10 no.4
• /
• pp.101-109
• /
• 2010

As global warming progresses, nations around the world are trying to reduce emission of $CO_2$ that accounts for the greatest portion of greenhouse gases. To reduce $CO_2$ emission, it is first necessary to estimate $CO_2$ emission of each industry. Government authorities estimate basic unit of $CO_2$ emission from re-bar that is one of the key materials of construction industry with LCA technique (Life Cycle Assessment). However, basic unit of $CO_2$ emission varies from organization to organization. The Ministry of Land, Transport and Maritime Affairs (2004) publishes it 3.48($TCO_2/ton$) and 0.30($TCO_2/ton$) with input-output analysis while the Korea Environmental Industry & Technology Institute (2008) defines it as 0.34($TCO_2/ton$) with process analysis, which indicates ambiguity in application of basic unit of $CO_2$emission. Based on the analysis of conventional methods used for estimating the $CO_2$ emission, therefore, this research suggests existing problems on the methods and focuses on proposing an strategy to effectively estimate the basic unit of $CO_2$ emission according to the energy consumption limited to the re-bar production in steel mill in order to overcome the problems. The result of this research is expected to be helpful in calculating and reducing $CO_2$ emission.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2017.05a
• /
• pp.194-197
• /
• 2017

Aircraft engine and auxiliary power units are certified in processes of design, production according to airworthiness requirements. The aircraft engines shall demonstrate compliance with the design and production through the Type Certificate and the auxiliary power unit shall demonstrate compliance with the design and production through the Technical Standards Order Approval. MOLIT(Ministry of Land, Infrastructure and Transport) established the law and the airworthiness requirements for aircraft engines such as KAS Part 33 but didn't issue the Technical Standards Order for auxiliary power unit. In this paper, we proposed and explained the plan for improvement of our aircraft engines industry through building and expanding the certification infra-structure for aircraft engines and auxiliary power unit.

• Mycobiology
• /
• v.39 no.2
• /
• pp.103-108
• /
• 2011

Amylases and cellulases are important enzymes that can be utilized for various biological activities. Ten different wild Nigerian mushrooms (Agaricus blazei, Agaricus sp., Corilopsis occidentalis, Coriolus versicolor, Termitomyces clypeatus, Termitomyces globulus, Pleurotus tuber-regium, Podoscypha bolleana, Pogonomyces hydnoides, and Nothopanus hygrophanus) were assayed for production of these secondary metabolites. The results revealed that most of the tested wild fungi demonstrated very good amylase and cellulase activities. With the incorporation of carboxymethyl-cellulose (a carbon source) into the culture medium, Agaricus blazei had the highest amylolytic activity of 0.60 unit/mL (at $25^{\circ}C$, pH 6.8). This was followed in order by P. tuber-regium and Agaricus sp. with 0.42 and 0.39 unit/mL, respectively ($p {\leq} 0.05$). Maltose and sucrose supplementation into the submerged liquid medium made N. hygrophanus and P. hydnoides to exhibit very low amylase activities of 0.09 and 0.11 unit/mL, respectively. Introducing peptone (an organic nitrogen source) into the basal medium enhanced the ability of C. versicolor to produce a cellulase value of 0.74 unit/mL. Other organic nitrogen sources that supported good cellulase activities were yeast extract and urea. Sodium nitrate (inorganic nitrogen source) generally inhibited cellulase production in all mushrooms. The best carbon source was carboxymethyl-cellulose, which promoted very high cellulase activity of 0.67 unit/mL in C. versicolor, which was followed in order by P. tuber-regium, T. chypeatus, and C. occidentalis ($p {\leq} 0.05$). Sucrose was the poorest carbon compound, supporting the lowest values of 0.01, 0.01, and 0.14 unit/mL in P. hydnoides, A. blazei, and Agaricus sp., respectively.