• Journal of Animal Science and Technology
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• 제57권5호
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• pp.17.1-17.6
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• 2015

The present study investigated the nutritional and economic suitability of cashew reject meal (full fat and defatted) as replacement for groundnut cake (GNC) in the diets of laying chickens. A total of eighty four brown shavers at 25 weeks of age were randomly allotted into seven dietary treatments each containing 6 replicates of 2 birds each. The seven diets prepared included diet 1, a control with GNC at $220gkg^{-1}$ as main protein source in the diet. Diets 2, 3 and 4 consist of gradual replacement of GNC with defatted cashew reject meal (DCRM) at 50%, 75% and 100% on weight for weight basis respectively while diets 5, 6 and 7 consist of gradual inclusion of full fat cashew reject meal (FCRM) to replace 25%, 35% and 50% of GNC protein respectively. Each group was allotted a diet in a completely randomized design in a study that lasted eight weeks during which records of the chemical constituent of the test ingredients, performance characteristics, egg quality traits and economic indicators were measured. Results showed that the crude protein were 22.10 and 35.4% for FCRM and DCRM respectively. Gross energy of DCRM was 5035 kcal/kg compared to GNC, 4752 kcal/kg. Result of aflatoxin $B_1$ revealed moderate level between 10 and $17{\mu}g/Kg$ in DCRM and GNC samples respectively. Birds on control gained 10 g, while those on DCRM and FCRM gained about 35 g and 120 g respectively. Feed intake declined (P < 0.05) with increased level of FCRM. Hen day production was highest in birds fed DCRM, followed by control and lowest value (P < 0.05) was recorded for FCRM. No significant change (P > 0.05) was observed for egg weight and shell thickness. Fat deposition and cholesterol content increased (P > 0.05) with increasing level of FCRM. The cost of feed per kilogram decreased gradually with increased inclusion level of CRM. The prediction equation showed the relative worth of DCRM compared to GNC was 92.3% whereas the actual market price of GNC triples that of DCRM. It was recommended that GNC could be completely replaced by DCRM in layer's diets in regions where this by product is abundant. However, FCRM should be cautiously used in diets of laying chickens.

• 농업생명과학연구
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• 제46권1호
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• pp.113-121
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• 2012

본 연구는 2005년에서 2009년까지 수집된 150,624개의 국내 젖소 산유능력 검정자료를 사용하여 두 가지 동기우 그룹 정의에 따른 산차, 체세포 점수가 305일로 보정된 유량, 유지방량, 유단백량, 무지고형분량에 미치는 효과와, 유량 및 유성분 간 상관관계에 변화가 나타나는가를 보고자 실시하였다. 첫 번째 동기우 그룹은 동일농장과 출생년도, 계절로 정의하였고, 두 번째는 농장과 분만년도, 계절을 동기우 그룹으로 정의하였다. 동기우 그룹을 출생년도로 구성하였을 때 동기우 그룹과 산차 및 체세포 점수가 유량과 고도의 유의성을 보였고, 유단백질량, 무지고형분량, 유지방량에 대해서도 유의적인 차이를 보였다. 동기우 그룹을 분만년도로 하였을 때에도 유량에 대한 체세포점수 및 산차 효과가 고도의 유의성을 보였고, 유단백질량, 유지방과 무지고형분량에 대해서도 고도의 유의성을 보였다. 출생년도를 사용한 모델에서 생산형질간 표현형상관을 본 결과 유량과 유단백량, 유지방, 무지고형분량은 고도의 정의 상관이 나타났으나, 유량과 체세포 점수 간에는 -0.09의 상관을 나타냈다. 분만년도를 동기우 그룹으로 사용한 모델에서의 표현형상관을 보면 유량과 유단백질 및 유지방간에 출생년도를 사용한 모델과 거의 비슷한 정의 상관을 보였고, 유량과 체세포간에는 -0.10, 유단백질, 유지방, 무지고형분량과 체세포간에는 각각 -0.08, -0.08, -0.11의 상관을 보였다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권12호
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• pp.1836-1843
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• 2019

Objective: Social genetic effects (SGE) are an important genetic component for growth, group productivity, and welfare in pigs. The present study was conducted to evaluate i) the feasibility of the single-step genomic best linear unbiased prediction (ssGBLUP) approach with the inclusion of SGE in the model in pigs, and ii) the changes in the contribution of heritable SGE to the phenotypic variance with different scaling ${\omega}$ constants for genomic relationships. Methods: The dataset included performance tested growth rate records (average daily gain) from 13,166 and 21,762 pigs Landrace (LR) and Yorkshire (YS), respectively. A total of 1,041 (LR) and 964 (YS) pigs were genotyped using the Illumina PorcineSNP60 v2 BeadChip panel. With the BLUPF90 software package, genetic parameters were estimated using a modified animal model for competitive traits. Giving a fixed weight to pedigree relationships (${\tau}:1$), several weights (${\omega}_{xx}$, 0.1 to 1.0; with a 0.1 interval) were scaled with the genomic relationship for best model fit with Akaike information criterion (AIC). Results: The genetic variances and total heritability estimates ($T^2$) were mostly higher with ssGBLUP than in the pedigree-based analysis. The model AIC value increased with any level of ${\omega}$ other than 0.6 and 0.5 in LR and YS, respectively, indicating the worse fit of those models. The theoretical accuracies of direct and social breeding value were increased by decreasing ${\omega}$ in both breeds, indicating the better accuracy of ${\omega}_{0.1}$ models. Therefore, the optimal values of ${\omega}$ to minimize AIC and to increase theoretical accuracy were 0.6 in LR and 0.5 in YS. Conclusion: In conclusion, single-step ssGBLUP model fitting SGE showed significant improvement in accuracy compared with the pedigree-based analysis method; therefore, it could be implemented in a pig population for genomic selection based on SGE, especially in South Korean populations, with appropriate further adjustment of tuning parameters for relationship matrices.

• 한국작물학회지
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• 제66권3호
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• pp.265-269
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• 2021

본 연구는 새로운 종자형 잔디 개발을 위한 유전자원의 분석을 통해 기초자료를 제공하고자 수행되었다. 우리나라에 자생하고 있는 조이시아그래스종 잔디는 주로 포복경이나 지하경을 통해 번식하며, 자연적으로 발아가 잘 되지 않아 종자형 육종을 잘 이루어지지 않고 있었다. 하지만 종피 처리 기술이 개발된 이후, 종자형 잔디 개발이 이루어지고 있다. 종자형 잔디를 개발하기 위해 확보하고 있는 잔디 유전자원에서 꽃대 형성률을 확인할 필요성이 생겼으며, 이 실험에서 549개 라인을 확인해 본 결과, 43개 라인, 즉 7.83%의 라인에서만 꽃대가 출현한 것으로 확인할 수 있었다. 상관분석을 실시 해 본 결과, 이식 후 생존률과 포복경 생성률은 유의하게 양의 상관관계(Rho = 0.44)를 보였고, 이러한 경향은 20% 이상 꽃대를 형성한 것으로 나타난 라인에서 상관관계가 더 높은 경향(Rho = 0.55)을 보였다. 하지만 이식 후 생존률과 포복경 생성률은 꽃대 출현과 상관관계가 매우 낮은 것으로 확인할 수 있었고(Rho = -0.11 and Rho = -0.06), 20% 이상의 꽃대 출현을 보인 43개 라인에서는 유의한 결과를 얻지 못하였다. 따라서 종자형 잔디 개발을 위해서는 이식 후 꽃대 출현률이 저조한 것을 감안, 더욱 많은 유전자원을 확보하는 것이 필요하며, 꽃대 출현이 늦게 나타나는 것을 고려하여 이것과 상관관계가 초기 확인 가능한 특질을 찾아, 꽃대 출현을 조기에 예측할 수 있는 기법이 요구된다.

• Animal Bioscience
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• 제34권12호
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• pp.1886-1894
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• 2021

Objective: Growth hormone (GH) and insulin-like growth factor I (IGF-I) play a critical role in animal growth rates. We aimed to investigate the effect of GH and IGF-I genotypes on body weight (BW), dominance, and gene expression in slow-growing chickens at different ages. Methods: A total of 613 Korat chickens (KRs) were bred and divided into three groups by genotype - A1A1, A1A3, and A3A3 for GH and AA, AC, and CC for IGF-I. Chickens were weighed every two weeks, and liver and breast muscle tissues were collected at 10 weeks of age. Genetic parameters of KRs were estimated using ASReml software. The GH and IGF-I mRNA levels were measured by quantitative polymerase chain reaction. Significant differences between traits were analyzed using the generalized linear model. Results: A significant effect of GH genotypes on BW was found at most ages, and the A1A1 genotype had the highest value of BW. Compared with the A3A3 genotype, the A1A1 and A1A3 genotypes showed a higher dominance effect at 0 and 2 weeks, and genotype A1A1 had the highest value of dominance at 8 weeks of age. A difference in GH mRNA levels between genotypes was detected in breast muscle at 6 weeks and in the liver tissue at 2 weeks. In the case of IGF-I gene, the AA genotype had the highest BW at the beginning of life. Significant differences in BW dominance were found at 2 weeks. However, IGF-I mRNA levels were not different among genotypes in both breast muscles and liver tissues. Conclusion: Our results revealed that GH and IGF-I influence growth, but may not be involved in heterosis. GH can be used as a marker gene in selection programs for growth because the homozygous genotype (A1A1) had the highest BW at all ages. The IGF-I is not a useful marker gene for selection programs.

• Animal Bioscience
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• 제34권11호
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• pp.1749-1756
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• 2021

Objective: Microminipig (MMP) is a miniature pig with an extra small body size for experimental use. In the present study, the occurrence of stillbirths and their genetic association with swine leukocyte antigen (SLA) class II haplotypes were evaluated in a population of MMPs. Methods: The occurrences of stillbirth and genetic association with SLA class II haplotypes using 483 stillborn and 2,246 live piglets, and their parents were compared among the three groups of newborn piglet litters; an all stillborn (AS) group consisting of only stillborn piglet litters, a partial stillborn (PS) group consisting of stillborn and live piglet litters, and an all alive (AA) group consisting of only live piglet litters. Results: The incidence of stillborn piglets was 483/2,729 (17.7%). Distributions of litter sizes, numbers of stillborn piglets in a litter, parities, and gestation periods were distinct among the three groups. The frequencies of low resolution haplotype (Lr)-0.7 or Lr-0.23 were higher in the AS group than in the PS or AA groups. In sires, the frequency of Lr-0.7 associated with the AS group was significantly higher in the AS group than with the AA group. In dams, the frequency of Lr-0.23 was significantly higher in the AS group than in the PS or AA groups, whereas the frequency of Lr-0.7 was not significantly different. Conclusion: The incidence of stillborn piglets in MMPs appears to be higher than those in other pig breeds. Several traits related with stillbirths such as the number of stillborn piglets and parities of the AS group were different from those of the PS and AA groups. Specific SLA class II haplotypes were associated significantly with a high incidence of stillbirths and could be used as genetic markers to adopt breeding strategies to lower the rate of stillbirth in MMPs.

• 한국육종학회지
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• 제43권4호
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• pp.311-317
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• 2011

이 실험은 다양한 크기의 종자로부터 콩나물을 키우고 콩나물의 생장 및 물리적 특성으로 조사함으로써 나물콩 품종육성과 나물콩 품질평가를 위한 기초자료를 얻고자 수행되었다. 대립 및 중소립 재배종 각각 3품종, 야생종과 재배종 교잡종 소립 3품종, 극소립 야생콩 3계통을 공시하여 4일간 콩나물을 키운 뒤 생육특성과 콩나물의 물성을 조사하였다. 콩나물 재배 96시간 후 배축 길이는 품종간 차이가 컸는데 황금콩, 풍산나물콩 및 소영이 가장 길었으며 극소립인 야생콩 계통들이 짧았다. 특히 대립종인 황금콩은 72시간까지 소립 나물콩 품종들과 비슷하게 생장하였으며 96시간 후에는 15.9 cm로 가장 길었다. 극소립 야생종계통은 재배 48시간 후부터 72시간 사이에 급격하게 증가하였으며 원료콩 1 g당 생장량도 월등히 많았으나 다른 품종들은 비슷한 증가양상을 보였다. 재배 96시간 후 단위 종자당 콩나물 생체중 증가비율은 소립일수록 컸으며 콩나물 수율도 소립종일수록 높은 경향을 보였다. 생체 배축의 hardness는 장엽콩이 3,505 g으로 가장 컸고 야생종 YWS516이 1,750 g으로 가장 낮았다. Mastication는 태광콩이 1,650 g으로 가장 높았으며 YWS516이 1,079g으로 가장 낮았으며 배축의 cutting force는 은하콩이 133 g으로 가장 크고 YWS516이 51 g으로 가장 낮았다. 익힌 후의 cutting force는 모든 품종에서 증가하였는데 전체적으로 종자크기가 클수록 물성특성이 증가하는 것으로 나타났다. 배축의 breaking force는 장엽콩이 83.5 g로 가장 컸고 야생콩이 가장 낮아 종자크기가 클 수록 증가하였다.

• Journal of Animal Science and Technology
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• 제60권12호
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• pp.32.1-32.10
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• 2018

Background: Necdin (NDN), a member of the melanoma antigen family showing imprinted pattern of expression, has been implicated as causing Prader-Willi symptoms, and known to participate in cellular growth, cellular migration and differentiation. The region where NDN is located has been associated to QTLs affecting reproduction and early growth in cattle, but location and functional analysis of the molecular mechanisms have not been established. Methods: Here we report the sequence variation of the entire coding sequence from 72 samples of cattle, yak, buffalo, goat and sheep, and discuss its variation in Bovidae. Median-joining network analysis was used to analyze the variation found in the species. Synonymous and non-synonymous substitution rates were determined for the analysis of all the polymorphic sites. Phylogenetic analysis were carried out among the species of Bovidae to reconstruct their relationships. Results: From the phylogenetic analysis with the consensus sequences of the studied Bovidae species, we found that only 11 of the 26 nucleotide changes that differentiate them produced amino acid changes. All the SNPs found in the cattle breeds were novel and showed similar percentages of nucleotides with non-synonymous substitutions at the N-terminal, MHD and C-terminal (12.3, 12.8 and 12.5%, respectively), and were much higher than the percentage of synonymous substitutions (2.5, 2.6 and 4.9%, respectively). Three mutations in cattle and one in sheep, detected in heterozygous individuals were predicted to be deleterious. Additionally, the analysis of the biochemical characteristics in the most common form of the proteins in each species show very little difference in molecular weight, pI, net charge, instability index, aliphatic index and GRAVY (Table 4) in the Bovidae species, except for sheep, which had a higher molecular weight, instability index and GRAVY. Conclusions: There is sufficient variation in this gene within and among the studied species, and because NDN carry key functions in the organism, it can have effects in economically important traits in the production of these species. NDN sequence is phylogenetically informative in this group, thus we propose this gene as a phylogenetic marker to study the evolution and conservation in Bovidae.

• 한국작물학회지
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• 제64권2호
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• pp.102-108
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• 2019

본 연구는 식용옥수수 풋이삭의 식감의 결정에 영향을 미치는 과피의 두께를 측정하는 방법을 규명하기 위하여 과피 시료의 준비 방법에 따른 과피 두께의 차이와 등숙 시기별 과피 두께의 변화를 조사하기 위하여 수행하였다. 1. 찰옥수수와 초당옥수수 각 3품종의 시료를 수확 직후 생옥수수(생시료) 알곡을 바로 측정하거나 상온의 음지에서 완전(건조시료) 건조시키거나 $-4^{\circ}C$로 냉동시킨 후 과피를 측정하였다. 2. 시료 준비 방법과 등숙 시기에 따른 삼원 교호작용의 유의성이 인정되어 어느 한 요인에 대한 일반적인 결론을 도출하기 보다는 각 시료 준비 방법과 특정 등숙 시기에 따른 과피 두께의 비교를 수행하였다. 3. 초당옥수수는 건조시료에서 등숙이 진행됨에 따라 비교적 안정된 과피 두께 측정값을 얻을 수 있었으며 찰옥수수는 비해 모든 시료 준비 방법에서 초당옥수수보다 안정된 값을 보였다. 특히 풋찰옥수수 수확 적기인 수정 후 24일경에는 시료 준비 방법에 따른 차이는 크게 나타나지 않을 것으로 기대된다.

• Journal of Animal Science and Technology
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• 제61권4호
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• pp.192-203
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• 2019

A study was conducted to determine the influence of feed form and microbial enzyme supplementation on energy utilization, bone quality, and amino acid and mineral digestibility of broiler chickens. Four hundred and eighty Ross 308, day-old broiler chickens were randomly assigned to eight diets formulated from commonly used ingredients in Tanzania. A 2 (pellet or mash) ${\times}$ 4 (control, Axtra XB, Quantum Blue (QB) and Axtra XB + QB enzyme) factorial array in a completely randomized design having six replicates per treatment (10 birds per replicate) was used. Birds were raised in climate-controlled rooms in a 3-phase; starter (0-10 days), grower (11-24 days) and finisher (25-35 days). Apparent metabolizable energy (AME), metabolizable energy intake, net energy of production, energy retained as protein (REp), and efficiency of metabolizable energy use for energy and protein retention were higher (p < 0.05) in birds fed pelleted diets. The AME and REp was higher (p < 0.05) with enzyme supplementation. Ash content, weight, length, width and breaking strength of tibia bones were highest (p < 0.05) in birds on pelleted diets. Tibia bone traits were improved (p < 0.05) when enzymes were included, particularly in a combination of QB and Axtra XB. However, potassium, magnesium, and zinc contents were highest (p < 0.05) when QB was supplemented. Digestibility of all amino acids was higher (p < 0.05) in birds supplied with pellets and with enzyme supplementation for most amino acids, except for serine. There was a positive interaction (p < 0.05) between feed form and enzymes on lysine and phenylalanine digestibility. Digestibility of Ca, P, K, S, Zn, and Fe was higher (p < 0.05) in birds fed pelleted diets, while those on mashed diets had higher (p < 0.05) digestibility of Cu and B. The digestibility of P, K, and Zn was highest (p < 0.001) when QB was added, while Ca, P, S, and B digestibility was highest when a combination of Axtra XB + QB was applied. Pelleted diets with or without enzymes improved energy utilization, digestibility of amino acids, and minerals, and increased bone strength in broiler chickens.